Type Mutant Stock; Targeted Mutation; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation ?+F1pN1
Generation DefinitionsDonating Investigator Oksana Lockridge, Eppley Inst. (Univ of Nebraska Med Cntr) Description
Transgenic "G117H BChE" mice are viable and fertile. These mice express a mutant form of human butyrylcholinesterase (BCHE or BChE), harboring a single amino acid change at codon 117 (His for Gly), under the control of the ROSA26 promoter. This mutation has the unusual ability to hydrolyze organophosphorus toxicants (OP), as well as acetylcholine and is resistant to inhibition by OP. All tested tissues show G117H BChE enzymatic activity. In the founder mice and immediate offspring, plasma concentrations of the mutant protein are approximately 25% of endogenous wildtype mouse BChe. No reported abnormalities result from BChE overexpression in homozygous mutant mice. Transgenic mice injected with the OP echothiophate are protected from the severe toxicity and lethality observed in wildtype controls. This is the first transgenic mouse strain that expresses human BChE as well as the first mammal with hereditary OP resistance. These G117H BChE transgenic mice may be useful for biodefense and bioterrorism studies involving organophosphorus toxicants (such as the nerve agents sarin, soman, and VX, the antiglaucoma drug echothiophate, and the active metabolite of the insecticide parathion (paraoxon)) or as a model of the human Cynthiana variant of BChE (conferring succinylcholine resistance).The donating investigator has multiple strains available that may be useful for testing toxic compounds, including the AChE-deficient (Stock No. 005987), G117H BChE transgenic (Stock No. 007577), and BChE-deficient (see Stock No. 008077 and Stock No. 008087) mice.
Development
The transgene was designed with a G117H mutant human butyrylcholinesterase (BCHE, or BChE) cDNA sequence downstream of the Gt(ROSA)26Sor promoter. In detail, the human G117H BChE sequence (encoding the 28 amino acid signal peptide and 574 amino acid full-length protein) was modified with a single His for Gly amino acid mutation at codon 117 (G117H). To enhance the expression of the human G117H BChE, a chimeric intron (consisting of a human beta-globin gene and the branch point and 3' acceptor site from the intron of an immunoglobulin gene heavy chain variable region) was placed between the promoter and human G117H BChE sequence. A FLAG epitope tag (encoding amino acids DYKDDDDK) was placed at the carboxy-terminus of the human G117H BChE. The transgene also contained the human elongation factor 1-alpha poly(A) sequence at the 3' end. This transgene was microinjected into fertilized [C57BL/6 x (C57BL/6xSJL)F1] eggs. Founder mice (line M837T) were bred to B6SJL/F1Tac mice. Mutant mice were next bred together and/or to wildtype mice (either C57BL/6 or 129X1) for an unknown number of generations prior to arrival at The Jackson Laboratory.
| Control | ||
|---|---|---|
| 100012 B6SJLF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
Strains carrying other alleles of Gt(ROSA)26Sor
002292 129-Gt(ROSA)26Sor/J 006053 129-Gt(ROSA)26Sortm1(CAG-EGFP)Luo/J 006067 129-Gt(ROSA)26Sortm2(CAG-Dsred2/EGFP)Luo/J 006041 129-Gt(ROSA)26Sortm3(CAG-EGFP/Dsred2)Luo/J 003310 129S-Gt(ROSA)26Sortm1Sor/J 009043 129S-Gt(ROSA)26Sortm3(CAG-luc)Tyj/J 007844 129S4/SvJae-Gt(ROSA)26Sortm2(FLP*)Sor/J 003946 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J 007689 129S4/SvJaeSor-Gt(ROSA)26Sortm4(attB/attP)Sor/J 017626 B6(Cg)-Gt(ROSA)26Sortm1(CAG-GFP/Eif2c2)Zjh/J 010633 B6(Cg)-Gt(ROSA)26Sortm1(CAG-taulacZ)Bene/J 008242 B6(Cg)-Gt(ROSA)26Sortm1(Ikbkb)Rsky/J 007676 B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J 006071 B6.129-Gt(ROSA)26Sortm1(CAG-EGFP)Luo/J 007708 B6.129-Gt(ROSA)26Sortm1(HD*103Q)Xwy/J 008463 B6.129-Gt(ROSA)26Sortm1(cre/ERT2)Tyj/J 008606 B6.129-Gt(ROSA)26Sortm1Joe/J 006080 B6.129-Gt(ROSA)26Sortm2(CAG-Dsred2/EGFP)Luo/J 006075 B6.129-Gt(ROSA)26Sortm3(CAG-EGFP/Dsred2)Luo/J 011008 B6.129P2(Cg)-Gt(ROSA)26Sortm1(tTA)Roos/J 009669 B6.129P2-Gt(ROSA)26Sortm1(DTA)Lky/J 008513 B6.129P2-Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde/J 013586 B6.129P2-Gt(ROSA)26Sortm1Nik/J 013587 B6.129P2-Gt(ROSA)26Sortm3Nik/J 009086 B6.129S4-Gt(ROSA)26Sortm1(FLP1)Dym/RainJ 003474 B6.129S4-Gt(ROSA)26Sortm1Sor/J 012930 B6.129S4-Gt(ROSA)26Sortm2(FLP*)Sor/J 009044 B6.129S4-Gt(ROSA)26Sortm3(CAG-luc)Tyj/J 007743 B6.129S4-Gt(ROSA)26Sortm3(phiC31*)Sor/J 009673 B6.129S6(C)-Gt(ROSA)26Sortm3(HIF1A*)Kael/J 002192 B6.129S7-Gt(ROSA)26Sor/J 006148 B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J 014588 B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1A1tm6(tetO-MSI2)Jae/J 006965 B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae/J 005670 B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J 007914 B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J 007920 B6.Cg-Gt(ROSA)26Sortm2(CAG-EYFP)Hze/J 012567 B6.Cg-Gt(ROSA)26Sortm27.1(CAG-COP4*H134R/tdTomato)Hze/J 007903 B6.Cg-Gt(ROSA)26Sortm3(CAG-EYFP)Hze/J 014648 B6.Cg-Gt(ROSA)26Sortm37(H1/tetO-RNAi:Taz)Arte/ZkhuJ 007906 B6.Cg-Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze/J 007909 B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J 007897 B6.Cg-Tg(Gt(ROSA)26Sor-EGFP)I1Able/J 017455 B6;129-Gt(ROSA)26Sortm1(CAG-COP4*E123T*H134R,-tdTomato)Gfng/J 010527 B6;129-Gt(ROSA)26Sortm1(DTA)Mrc/J 016262 B6;129-Gt(ROSA)26Sortm1(Foxo1/GFP)Jke/J 008883 B6;129-Gt(ROSA)26Sortm1(SNCA*A53T)Djmo/TmdJ 004847 B6;129-Gt(ROSA)26Sortm1(cre/ERT)Nat/J 006911 B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm2(tetO-Pou5f1)Jae/J 008516 B6;129-Gt(ROSA)26Sortm1Joe/J 003504 B6;129-Gt(ROSA)26Sortm1Sho/J 008889 B6;129-Gt(ROSA)26Sortm2(SNCA*119)Djmo/TmdJ 009253 B6;129-Gt(ROSA)26Sortm2Nat/J 004077 B6;129-Gt(ROSA)26Sortm2Sho/J 008886 B6;129-Gt(ROSA)26Sortm3(SNCA*E46K)Djmo/TmdJ 010557 B6;129-Gt(ROSA)26Sortm3(rtTA,tetO-cre/ERT)Nat/J 010523 B6;129P2-Gt(ROSA)26Sortm1(CAG-ALPP)Fawa/J 002073 B6;129S-Gt(ROSA)26Sor/J 012569 B6;129S-Gt(ROSA)26Sortm32(CAG-COP4*H134R/EYFP)Hze/J 012570 B6;129S-Gt(ROSA)26Sortm34.1(CAG-Syp/tdTomato)Hze/J 012735 B6;129S-Gt(ROSA)26Sortm35.1(CAG-AOP3/GFP)Hze/J 014538 B6;129S-Gt(ROSA)26Sortm38(CAG-GCaMP3)Hze/J 014539 B6;129S-Gt(ROSA)26Sortm39(CAG-HOP/EYFP)Hze/J 016836 B6;129S4-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm7(tetO-HIST1H2BJ/GFP)Jae/J 003309 B6;129S4-Gt(ROSA)26Sortm1Sor/J 004598 B6;129S4-Gt(ROSA)26Sortm2Dym/J 007670 B6;129S4-Gt(ROSA)26Sortm3(phiC31*)Sor/J 016999 B6;129S6-Gt(ROSA)26Sortm1(xstpx-rtTA2S*M2)Whsu/J 007908 B6;129S6-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J 007905 B6;129S6-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J 009670 C.129P2(B6)-Gt(ROSA)26Sortm1(DTA)Lky/J 008603 C.129P2(B6)-Gt(ROSA)26Sortm1(tTA)Roos/J 002955 C.129S7-Gt(ROSA)26Sor/J 007900 C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai/J 008517 C57BL/6-Gt(ROSA)26Sortm3(CAG-MIR17-92,-EGFP)Rsky/J 012637 C57BL/6-Gt(ROSA)26Sortm5(Map3k14)Rsky/J 012638 C57BL/6-Gt(ROSA)26Sortm6(Map3k14*)Rsky/J 012343 C57BL/6-Gt(ROSA)26Sortm7(Pik3ca*,EGFP)Rsky/J 012352 C57BL/6-Gt(ROSA)26Sortm8(Map2k1*,EGFP)Rsky/J 012361 C57BL/6-Gt(ROSA)26Sortm9(Rac1*,EGFP)Rsky/J 005420 C;129S7 Gt(ROSA)26Sor-Bmp5cfe-se7J/J 008040 CBy.B6-Gt(ROSA)26Sortm1(HBEGF)Awai/J 007898 CBy.Cg-Tg(Gt(ROSA)26Sor-EGFP)I1Able/J 009427 FVB.129S4(B6)-Gt(ROSA)26Sortm1Sor/J 005125 FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J 006206 FVB.129S6-Gt(ROSA)26Sortm2(HIF1A/luc)Kael/J 012429 FVB.Cg-Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh/J 010920 FVB;129P2-Gt(ROSA)26Sortm1(birA)Mejr/J 016603 NOD.B6-Gt(ROSA)26Sortm1(HBEGF)Awai/DvsJ 013731 STOCK Gt(ROSA)26Sortm1(CAG-Brainbow2.1)Cle/J 006331 STOCK Gt(ROSA)26Sortm1(DTA)Jpmb/J 008159 STOCK Gt(ROSA)26Sortm1(Notch1)Dam/J 005130 STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J 011004 STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm3(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae/J 011011 STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm4(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae/J 011013 STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm5(tetO-Pou5f1,-Klf4,-Myc)Jae/J 005572 STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J 008600 STOCK Gt(ROSA)26Sortm1(tTA)Roos/J 013124 STOCK Gt(ROSA)26Sortm3(Gli3)Amc/J 007576 STOCK Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J 009674 STOCK Gt(ROSA)26Sortm4(HIF2A*)Kael/J 012266 STOCK Gt(ROSA)26Sortm5(ACTB-tTA)Luo/J 013123 STOCK Gt(ROSA)26Sortm6(Gli1)Amc/J 007896 STOCK Tg(Gt(ROSA)26Sor-EGFP)I1Able/J View Strains carrying other alleles of Gt(ROSA)26Sor (104 strains)
View Mammalian Phenotype Terms
Mammalian Phenotype Terms provided by MGI
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Gt(ROSA)26Sor-BCHE*G117H)837Loc/Tg(Gt(ROSA)26Sor-BCHE*G117H)837Loc
involves: C57BL/6 * SJL
- homeostasis/metabolism phenotype
- abnormal enzyme/ coenzyme level
- F5 generation mice express human butyrylcholinesterase BCHE protein at 25% the level of the endogenous mouse protein (MGI Ref ID J:89332)
View Research Applications
Research Applications
This mouse can be used to support research in many areas including:
Cancer Research
Toxicology
Metabolism Research
Neurobiology Research
Neuromuscular Defects
Neurotransmitter Receptor and Synaptic Vesicle Defects
Receptor Defects
cholinergic receptor
Tremor Defects
Research Tools
Cancer Research
Metabolism Research
Neurobiology Research
Sensorineural Research
Toxicology Research
drug metabolism
drug/compound testing
| Allele Symbol | Tg(Gt(ROSA)26Sor-BCHE*G117H)837Loc | ||
|---|---|---|---|
| Allele Name | transgene insertion 837, Oksana Lockridge | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | G117H BChE; M844T; | ||
| Strain of Origin | C57BL/6 x (C57BL/6 x SJL)F1 | ||
| Expressed Gene | BCHE, butyrylcholinesterase, human | ||
| Promoter | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano, mouse, laboratory | ||
| Molecular Note | The human G117H BChE sequence (encoding the 28 amino acid signal peptide and 574 amino acid full-length protein) was modified with a single His for Gly amino acid mutation at codon 117 (G117H). To enhance expression, a chimeric intron (consisting of a human beta-globin gene and the branch and 3' acceptor site from the intron of an immunoglobulin gene heavy chain variable region) was placed between the promoter and human G117H BChE sequence. A FLAG epitope tag (encoding amino acids DYKDDDDK) was placed atthe carboxy-terminus of the human G117H BChE. The transgene also contained the human elongation factor 1-alpha poly(A) sequence at the 3' end. This transgene was microinjected into fertilized [C57BL/6 x (C57BL/6xSJL)F1] eggs. Founder mice (line M837T) were bred to B6SJL/F1Tac mice. All tested tissues express G117H BChE enzyme activity. In addition, 2 other stable lines M844T and M853T were generated, with detailed analysis of M844T described. [MGI Ref ID J:89332] | ||
| Gene Symbol and Name | Tg(Gt(ROSA)26Sor-BCHE*G117H)837Loc, transgene insertion 837, Oksana Lockridge | ||
| Chromosome | UN | ||
| Gene Common Name(s) | G117H BChE; M844T; | ||
Genotyping Protocols
Tg(Gt(ROSA)26Sor-BCHE*G117H)837Loc, QPCR
Tg(Gt(ROSA)26Sor-BCHE*G117H)837Loc, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
Wang Y; Boeck AT; Duysen EG; Van Keuren M; Saunders TL; Lockridge O. 2004. Resistance to organophosphorus agent toxicity in transgenic mice expressing the G117H mutant of human butyrylcholinesterase. Toxicol Appl Pharmacol 196(3):356-66. [PubMed: 15094306] [MGI Ref ID J:89332]
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred.
| Pricing for USA, Canada and Mexico shipping destinations |
|
![]() |
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
![]() |
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
|
|
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
| Control | ||
|---|---|---|
| 100012 B6SJLF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.