Strain Name:

B6;129-Igkctm1(IGKC)Mnz/J

Stock Number:

007595

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6;129-Igk-Ctm1(IGKC)Mnz/J    (Changed: 30-NOV-10 )
Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
GenerationN?pN1
Generation Definitions
 
Donating Investigator Michel Nussenzweig,   The Rockefeller University

Description
Mice homozygous for this targeted replacement of the mouse gene with the human homolog are viable and fertile and do not display any gross physical or behavioral abnormalities. Close to 50% of all B cells in heterozygous mice express human immunoglobulin kappa light chain constant region (Ck), detectable by immunostaining. This strain has been used to determine the extent of light chain receptor editing in the mouse by monitoring the replacement of kappa light chain that carries human constant region.

Development
A targeting vector containing the human immunoglobulin kappa chain constant region (hCk, IGCK), a floxed neomycin cassette (5'), and a polymerase II promoter driven diptheria toxin gene (DTA)(3') was used to replace the homologous mouse locus. The vector was introduced to a 129Sv embryonic stem (ES) cell line. Mice carrying the mutation were crossed to a C57BL/6 background EIIA-Cre strain to remove the floxed neomycin cassette. This strain has was maintained on a mixed 129 and C57BL/6 background by the donating laboratory.

Related Strains

Strains carrying other alleles of Igkc
002400   C57BL/6-Igkctm1Cgn/J
View Strains carrying other alleles of Igkc     (1 strain)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Immunoglobulin Kappa Light Chain Deficiency; IGKCD   (IGKC)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Igkctm1(IGKC)Mnz/Igkc+

        Background Not Specified
  • no phenotypic analysis
  • *normal* no phenotypic analysis
    • these mice were used to examine B cell receptor editing   (MGI Ref ID J:115079)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Igkctm1(IGKC)Mnz
Allele Name targeted mutation 1, Michel C Nussenzweig
Allele Type Targeted
Common Name(s) Igkappah; hCkappa;
Expressed Gene IGKC, immunoglobulin kappa constant, human
Molecular Note The mouse immunoglobulin kappa chain, constant region was replaced with the human IGKC coding sequence. A floxed neo selection cassette was subsequently removed from properly targeted mice by cre excision. [MGI Ref ID J:115079]
 
Gene Symbol and Name Igkc, immunoglobulin kappa constant
Chromosome 6
Gene Common Name(s) Igk-C; immunoglobulin kappa chain, constant region;

Genotyping

Genotyping Information

Genotyping Protocols

Igk-Ctm1(IGKC)Mnz, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Casellas R; Shih TA; Kleinewietfeld M; Rakonjac J; Nemazee D; Rajewsky K; Nussenzweig MC. 2001. Contribution of receptor editing to the antibody repertoire. Science 291(5508):1541-4. [PubMed: 11222858]  [MGI Ref ID J:115079]

Additional References

Igkctm1(IGKC)Mnz related

Ait-Azzouzene D; Gavin AL; Skog P; Duong B; Nemazee D. 2006. Effect of cell:cell competition and BAFF expression on peripheral B cell tolerance and B-1 cell survival in transgenic mice expressing a low level of Igkappa-reactive macroself antigen. Eur J Immunol 36(4):985-96. [PubMed: 16511898]  [MGI Ref ID J:114825]

Amin RH; Cado D; Nolla H; Huang D; Shinton SA; Zhou Y; Hardy RR; Schlissel MS. 2009. Biallelic, ubiquitous transcription from the distal germline Ig{kappa} locus promoter during B cell development. Proc Natl Acad Sci U S A 106(2):522-7. [PubMed: 19116268]  [MGI Ref ID J:143876]

Andrews SF; Zhang Q; Lim S; Li L; Lee JH; Zheng NY; Huang M; Taylor WM; Farris AD; Ni D; Meng W; Luning Prak ET; Wilson PC. 2013. Global analysis of B cell selection using an immunoglobulin light chain-mediated model of autoreactivity. J Exp Med 210(1):125-42. [PubMed: 23267014]  [MGI Ref ID J:194594]

Doyle-Cooper C; Hudson KE; Cooper AB; Ota T; Skog P; Dawson PE; Zwick MB; Schief WR; Burton DR; Nemazee D. 2013. Immune tolerance negatively regulates B cells in knock-in mice expressing broadly neutralizing HIV antibody 4E10. J Immunol 191(6):3186-91. [PubMed: 23940276]  [MGI Ref ID J:205764]

Duong BH; Ota T; Aoki-Ota M; Cooper AB; Ait-Azzouzene D; Vela JL; Gavin AL; Nemazee D. 2011. Negative selection by IgM superantigen defines a B cell central tolerance compartment and reveals mutations allowing escape. J Immunol 187(11):5596-605. [PubMed: 22043016]  [MGI Ref ID J:193234]

Fournier EM; Velez MG; Leahy K; Swanson CL; Rubtsov AV; Torres RM; Pelanda R. 2012. Dual-reactive B cells are autoreactive and highly enriched in the plasmablast and memory B cell subsets of autoimmune mice. J Exp Med 209(10):1797-812. [PubMed: 22927551]  [MGI Ref ID J:192933]

Inlay MA; Lin T; Gao HH; Xu Y. 2006. Critical roles of the immunoglobulin intronic enhancers in maintaining the sequential rearrangement of IgH and Igk loci. J Exp Med 203(7):1721-32. [PubMed: 16785310]  [MGI Ref ID J:124407]

Makdasi E; Eilat D. 2013. L chain allelic inclusion does not increase autoreactivity in lupus-prone new zealand black/new zealand white mice. J Immunol 190(4):1472-80. [PubMed: 23319731]  [MGI Ref ID J:193319]

Schram BR; Tze LE; Ramsey LB; Liu J; Najera L; Vegoe AL; Hardy RR; Hippen KL; Farrar MA; Behrens TW. 2008. B cell receptor Basal signaling regulates antigen-induced Ig light chain rearrangements. J Immunol 180(7):4728-41. [PubMed: 18354197]  [MGI Ref ID J:133383]

Xiang Y; Park SK; Garrard WT. 2013. Vkappa Gene Repertoire and Locus Contraction Are Specified by Critical DNase I Hypersensitive Sites within the Vkappa-Jkappa Intervening Region. J Immunol 190(4):1819-26. [PubMed: 23296705]  [MGI Ref ID J:193015]

Xiang Y; Zhou X; Hewitt SL; Skok JA; Garrard WT. 2011. A multifunctional element in the mouse Igkappa locus that specifies repertoire and Ig loci subnuclear location. J Immunol 186(9):5356-66. [PubMed: 21441452]  [MGI Ref ID J:172855]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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