Strain Name: |
B6;129-Igk-Ctm1(IGKC)Mnz/J |
|---|---|
Stock Number: |
007595 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Igk-C; Igk-Ctm1(IGKC)Mnz; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator Michel Nussenzweig, The Rockefeller University Strain Description
Mice homozygous for this targeted replacement of the mouse gene with the human homolog are viable and fertile and do not display any gross physical or behavioral abnormalities. Close to 50% of all B cells in heterozygous mice express human immunoglobulin kappa light chain constant region (Ck), detectable by immunostaining. This strain has been used to determine the extent of light chain receptor editing in the mouse by monitoring the replacement of kappa light chain that carries human constant region.Strain Development
A targeting vector containing the human immunoglobulin kappa chain constant region (hCk, IGCK), a floxed neomycin cassette (5'), and a polymerase II promoter driven diptheria toxin gene (DTA)(3') was used to replace the homologous mouse locus. The vector was introduced to a 129Sv embryonic stem (ES) cell line. Mice carrying the mutation were crossed to a C57BL/6 background EIIA-Cre strain to remove the floxed neomycin cassette. This strain has was maintained on a mixed 129 and C57BL/6 background by the donating laboratory.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Igk-Ctm1(IGKC)Mnz | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Michel C Nussenzweig | ||
| Common Name(s) | Igkappah; hCkappa; | ||
| Gene Symbol and Name | Igk-C, immunoglobulin kappa chain, constant region | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | HCAK1; Igkc; Km; MGC111575; MGC62011; MGC72072; MGC88770; MGC88771; MGC88809; | ||
| Molecular Note | The mouse immunoglobulin kappa chain, constant region was replaced with the human IGKC coding sequence. [MGI Ref ID J:115079] | ||
Genotyping Protocols
Igk-Ctm1(IGKC)Mnz
Related Strains
Strains carrying other alleles of Igk-C
002400 C57BL/6-Igk-Ctm1Cgn/J 005018 NOD.Cg-Tg(Igk-C/Igk-V125)1Jwt/JwtJ View Strains carrying other alleles of Igk-C (2 strains)
Research Applications
This mouse can be used to support research in many areas including:
Igk-C relatedImmunology and Inflammation Research
Immunology and Inflammation Research
Autoimmunity
CD Antigens, Antigen Receptors, and Histocompatibility Markers
References
Selected Reference(s)
Additional ReferencesCasellas R; Shih TA; Kleinewietfeld M; Rakonjac J; Nemazee D; Rajewsky K; Nussenzweig MC. 2001. Contribution of receptor editing to the antibody repertoire. Science 291(5508):1541-4. [PubMed: 11222858] [MGI Ref ID J:115079]
Price and Supply Information
| Strain Name: | B6;129-Igk-Ctm1(IGKC)Mnz/J |
| Stock Number: | 007595 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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