Description

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System +/+ sibling x Heterozygote         (Female x Male) Species laboratory mouse   Donating Investigator IMR Colony,   The Jackson Laboratory

Description
Mice homozygous for the Isl2^DTA targeted mutation are viable and fertile. These mice have the diphtheria toxin (DTA) gene inserted into the Isl2 (insulin related protein 2 (islet 2)) locus. Expression of DTA in Isl2-expressing cells is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTA expression and subsequent cell ablation.

When bred to a strain expressing Cre recombinase in motor neurons (see Stock No. 006600 for example), this mutant mouse strain may be useful in neurodevelopmental studies.

These Isl2^DTA mutant mice are available on a STOCK genetic background (Stock No. 007603), as well as a C57BL/6J-backcrossed background (Stock No. 007942).

Development
An internal ribosome entry site (IRES), loxP-flanked STOP sequence (consisting of a PGK-Neo gene in reverse orientation), and diphtheria toxin (DTA) coding sequence were inserted into the 3' UTR of the targeted gene. Mutant mice on an undefined genetic background were sent to The Jackson Laboratory.

Control Information

	Control
	Wild-type from the colony
Considerations for Choosing Controls

Additional Web Information

Cre-lox Systems

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX^® Services jaxservices@jax.org for customized breeding options.

Mnx1^tm4(cre)Tmj/Mnx1⁺ Isl2^tm1Arbr/Isl2⁺

        involves: 129S7/SvEvBrd * C57BL/6J   (conditional)

• nervous system phenotype
• abnormal motor neuron morphology (MGI Ref ID J:76364)
  • mice lack motor neurons when examined from E12 to E16.9

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Cre-lox System (loxP-flanked Sequences)

Research Tools
Cardiovascular Research (Cre-lox System)
Cell Biology Research
Cre-lox System (loxP-flanked Sequences)
Developmental Biology Research (Cre-lox System)
Diabetes and Obesity Research (loxP)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Neurobiology Research

Genes & Alleles

Gene & Allele Information

Allele Symbol		Isl2tm1Arbr
Allele Name		targeted mutation 1, Sylvia Arber
Allele Type		Targeted (knock-in)
Common Name(s)		Isl2DTA;
Mutation Made By		Steven Burden,   New York University Medical Center
Gene Symbol and Name		Isl2, insulin related protein 2 (islet 2)
Chromosome		9
Gene Common Name(s)		3110001N10Rik; FLJ10160; RIKEN cDNA 3110001N10 gene; islet 2; islet-2;
Molecular Note		An IRES-loxP-stop-loxP-DTA (diptheria toxin) cassette was inserted into the sequences encoding the 3'UTR of the gene. [MGI Ref ID J:69623]

Genotyping

Genotyping Information

Genotyping Protocols

Isl2^tm1Arbr, SEP PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Yang X; Arber S; William C; Li L; Tanabe Y; Jessell TM; Birchmeier C; Burden SJ. 2001. Patterning of muscle acetylcholine receptor gene expression in the absence of motor innervation. Neuron 30(2):399-410. [PubMed: 11395002]  [MGI Ref ID J:69623]

Additional References

Isl2^tm1Arbr related

Hippenmeyer S; Huber RM; Ladle DR; Murphy K; Arber S. 2007. ETS transcription factor Erm controls subsynaptic gene expression in skeletal muscles. Neuron 55(5):726-40. [PubMed: 17785180]  [MGI Ref ID J:126807]

Jevsek M; Jaworski A; Polo-Parada L; Kim N; Fan J; Landmesser LT; Burden SJ. 2006. CD24 is expressed by myofiber synaptic nuclei and regulates synaptic transmission. Proc Natl Acad Sci U S A 103(16):6374-9. [PubMed: 16606832]  [MGI Ref ID J:109028]

Kim N; Burden SJ. 2008. MuSK controls where motor axons grow and form synapses. Nat Neurosci 11(1):19-27. [PubMed: 18084289]  [MGI Ref ID J:129239]

Pun S; Sigrist M; Santos AF; Ruegg MA; Sanes JR; Jessell TM; Arber S; Caroni P. 2002. An intrinsic distinction in neuromuscular junction assembly and maintenance in different skeletal muscles. Neuron 34(3):357-70. [PubMed: 11988168]  [MGI Ref ID J:76364]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, homozygous mice may be bred.
Mating System	+/+ sibling x Heterozygote         (Female x Male)
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	Wild-type from the colony
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.