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Genes & Alleles

Isl2;   Isl2tm1Arbr;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System +/+ sibling x Heterozygote         (Female x Male) Species laboratory mouse Donating Investigator IMR Colony,   The Jackson Laboratory

Strain Description
Mice homozygous for the Isl2^DTA targeted mutation are viable and fertile. These mice have the diphtheria toxin (DTA) gene inserted into the Isl2 (insulin related protein 2 (islet 2)) locus. Expression of DTA in Isl2-expressing cells is blocked by an upstream loxP-flanked STOP sequence. When bred to Cre recombinase-expressing mice, the STOP sequence is deleted in tissues where Cre is present, permitting DTA expression and subsequent cell ablation.

When bred to a strain expressing Cre recombinase in motor neurons (see Stock No. 006600 for example), this mutant mouse strain may be useful in neurodevelopmental studies.

These Isl2^DTA mutant mice are available on a STOCK genetic background (Stock No. 007603), as well as a C57BL/6J-backcrossed background (Stock No. 007942).

Strain Development
An internal ribosome entry site (IRES), loxP-flanked STOP sequence (consisting of a PGK-Neo gene in reverse orientation), and diphtheria toxin (DTA) coding sequence were inserted into the 3' UTR of the targeted gene. Mutant mice on an undefined genetic background were sent to The Jackson Laboratory.

Mammalian Phenotype Terms assigned by genotype The following phenotype relates to a compound genotype created using this strain. Contact JAX® Services jaxservices@jax.org for customized breeding options. Mnx1tm4(cre)Tmj/Mnx1+ Isl2tm1Arbr/Isl2+         involves: 129S7/SvEvBrd * C57BL/6J   (conditional) nervous system phenotype abnormal motor neuron morphology (MGI Ref ID J:76364) mice lack motor neurons when examined from E12 to E16.9

Gene & Allele Details

Allele Symbol		Isl2tm1Arbr
Allele Name		targeted mutation 1, Sylvia Arber
Common Name(s)		Isl2DTA;
Mutation Made By		Steven Burden,   New York University Medical Center
Gene Symbol and Name		Isl2, insulin related protein 2 (islet 2)
Chromosome		9
Gene Common Name(s)		3110001N10Rik; FLJ10160; RIKEN cDNA 3110001N10 gene; islet 2; islet-2;
Molecular Note		An IRES-loxP-stop-loxP-DTA (diptheria toxin) cassette was inserted into the sequences encoding the 3'UTR of the gene. [MGI Ref ID J:69623]

Control Information

	Control
	Wild-type from the colony
Considerations for Choosing Controls

Genotyping Protocols

Isl2^tm1Arbr

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, homozygous mice may be bred.
Diet Information	LabDiet® 5K52/5K67

Additional Web Information

Cre-lox or FLP-FRT Systems

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Cre-lox System (loxP-flanked Sequences)

Research Tools
Cardiovascular Research (Cre-lox System)
Cell Biology Research
Cre-lox System (loxP-flanked Sequences)
Developmental Biology Research (Cre-lox System)
Diabetes and Obesity Research (loxP)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Neurobiology Research

References

Selected Reference(s)

Yang X; Arber S; William C; Li L; Tanabe Y; Jessell TM; Birchmeier C; Burden SJ. 2001. Patterning of muscle acetylcholine receptor gene expression in the absence of motor innervation. Neuron 30(2):399-410. [PubMed: 11395002]  [MGI Ref ID J:69623]

Additional References

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Price and Supply Information

Strain Name:	STOCK Isl2tm1Arbr/J
Stock Number:	007603

Price Details

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Supply Details

Standard Supply	Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes	Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.
Licensing	See General Terms and Conditions below for Licensing and Use Restrictions
Control Information	View Control Information in Strain Details.

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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