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| These PS1-floxed mice may be useful in generating conditional knockouts of Presenilin 1 for studying Alzheimer’s Disease. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B6.129P-Psen1tm1Vln/J (Changed: 29-AUG-08 ) B6;129P-Psen1tm1Vln/J (Changed: 07-SEP-07 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N8+F3pN1
Generation DefinitionsDonating Investigator Fred Van Leuven, Katholieke Universiteit Leuven Description
These mice possess loxP sites on either side of exon 7 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these "floxed" mice are bred to mice that express Cre recombinase, resulting offspring can have one of three resulting genotypes (only exon 7 deleted, only the neo selection cassette deleted, or both exon 7 and the neo selection cassette deleted) in the cre-expressing tissue(s). These PS1-floxed mice may be useful in generating conditional knockouts of Presenilin 1 for studying Alzheimer's Disease.For example, when crossed to a strain expressing Cre recombinase in postnatal neurons (see Stock No. 006143), this mutant mouse strain may be useful in studies of amyloid plaque formation.
Development
A loxP site flanked targeting vector containing a neomycin resistance gene was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 7 of the targeted gene, and another loxP site was inserted upstream of exon 7. This construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES). Correctly targeted ES cells were injected into recipient blastocysts. The donating investigator reports that these mice were then backcrossed to C57BL/6 for 7 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred with C57BL/6J for one generation to establish the colony (see note below).NOTE: A 27 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory revealed a single loci that typed as homozygous for 129/P strain type (chromosome 12 approximately 9.8 cM) and three additional loci that were segregating for either C57BL/6J or 129/P strain type (chromosome 1 approximately 52.0 cM, chromosome 4 approximately 10.9 cM, and chromosome 8 approximately 4.4 cM). Therefore, these mice are determined to be on a mixed B6;129P genetic background (August 2008).
Control Information
| Control | ||
|---|---|---|
| 100492 B6129PF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Alzheimer's Disease Models
View Alzheimer's Disease Models (108 strains)
007685 B6.129P2-Psen1tm1Vln/J
004193 B6.129-Psen1tm1Mpm/J 003615 B6.129-Psen1tm1Shn/J 004807 B6;129-Psen1tm1Mpm Tg(APPSwe,tauP301L)1Lfa/Mmjax 003822 B6;129S-Psen1tm1Shn/J
Additional Web Information
Introduction to Cre-lox technology
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Phenotype
Phenotype Information
- Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).
Alzheimer Disease 3- Model with phenotypic similarity to human disease where etiologies are distinct. Human genes are associated with this disease. Orthologs of these genes do not appear in the mouse genotype(s).
Alzheimer Disease; AD
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Acne Inversa, Familial, 3; ACNINV3 (PSEN1)
Cardiomyopathy, Dilated, 1u; CMD1U (PSEN1)
Frontotemporal Dementia; FTD (PSEN1)
Pick Disease of Brain (PSEN1)
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Psen1tm1Vln/Psen1tm1Vln
Background Not Specified
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:87229)
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Psen1tm1Vln/Psen1tm1Vln Tg(Thy1-cre)1Vln/0
involves: FVB/N (conditional)
- behavior/neurological phenotype
- *normal* behavior/neurological phenotype
- mice do not display any behavioral or cognitive deficits (MGI Ref ID J:87229)
- nervous system phenotype
- *normal* nervous system phenotype
- brains of 6 month-old mice do not show any morphological abnormalities like cerebral hemorrhages, cavities or tumors; no defects are observed up to 2 years of age (MGI Ref ID J:87229)
- abnormal long term potentiation
- initial phase of the slope of fEPSP is lower (168% vs 221% in controls) 15 minutes after tetanic stimulation; slope of fEPSP progressively increases to approach control levels 2 hours following stimulation (MGI Ref ID J:87229)
- amyloid beta deposits
- levels of amyloid beta-40 and -42 (Abeta40, Abeta42) are reduced relative to controls; C-terminal fragments of APP accumulate in brains (MGI Ref ID J:87229)
- other phenotype
- amyloid beta deposits
- levels of amyloid beta-40 and -42 (Abeta40, Abeta42) are reduced relative to controls; C-terminal fragments of APP accumulate in brains (MGI Ref ID J:87229)
This mouse can be used to support research in many areas including:
Mouse/Human Gene Homologs
Alzheimer's
Neurobiology Research
Alzheimer's Disease
Presenilin mutants
Behavioral and Learning Defects
Cre-lox System
loxP-flanked Sequences
Research Tools
Cre-lox System
loxP-flanked Sequences
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Psen1tm1Vln | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Fred Van Leuven | ||
| Allele Type | Targeted (Floxed/Frt) | ||
| Common Name(s) | PS1-flox; | ||
| Mutation Made By | Fred Van Leuven, Katholieke Universiteit Leuven | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Gene Symbol and Name | Psen1, presenilin 1 | ||
| Chromosome | 12 | ||
| Gene Common Name(s) | AD3; Ad3h; FAD; PS-1; PS1; S182; alzheimer disease 3 homolog; presenilin-1; | ||
| Molecular Note | Exon 7 was flanked by a single loxP site in intron 6 and floxed neo cassette in intron 7. [MGI Ref ID J:87229] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Psen1tm1Vin, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Dewachter I; Reverse D; Caluwaerts N; Ris L; Kuiperi C; Van den Haute C; Spittaels K; Umans L; Serneels L; Thiry E; Moechars D; Mercken M; Godaux E; Van Leuven F. 2002. Neuronal deficiency of presenilin 1 inhibits amyloid plaque formation and corrects hippocampal long-term potentiation but not a cognitive defect of amyloid precursor protein [V717I] transgenic mice. J Neurosci 22(9):3445-53. [PubMed: 11978821] [MGI Ref ID J:87229]
Additional References
Psen1tm1Vln relatedDewachter I; Ris L; Croes S; Borghgraef P; Devijver H; Voets T; Nilius B; Godaux E; Van Leuven F. 2008. Modulation of synaptic plasticity and Tau phosphorylation by wild-type and mutant presenilin1. Neurobiol Aging 29(5):639-52. [PubMed: 17222948] [MGI Ref ID J:137684]
Herms J; Schneider I; Dewachter I; Caluwaerts N; Kretzschmar H; Van Leuven F. 2003. Capacitive calcium entry is directly attenuated by mutant presenilin-1, independent of the expression of the amyloid precursor protein. J Biol Chem 278(4):2484-9. [PubMed: 12431992] [MGI Ref ID J:81726]
Jung CK; Fuhrmann M; Honarnejad K; Van Leuven F; Herms J. 2011. Role of presenilin1 in structural plasticity of cortical dendritic spines in vivo. J Neurochem 119(5):1064-73. [PubMed: 21951279] [MGI Ref ID J:178473]
Ris L; Dewachter I; Reverse D; Godaux E; Van Leuven F. 2003. Capacitative calcium entry induces hippocampal long term potentiation in the absence of presenilin-1. J Biol Chem 278(45):44393-9. [PubMed: 12902342] [MGI Ref ID J:86553]
Tamboli IY; Prager K; Thal DR; Thelen KM; Dewachter I; Pietrzik CU; St George-Hyslop P; Sisodia SS; De Strooper B; Heneka MT; Filippov MA; Muller U; van Leuven F; Lutjohann D; Walter J. 2008. Loss of gamma-secretase function impairs endocytosis of lipoprotein particles and membrane cholesterol homeostasis. J Neurosci 28(46):12097-106. [PubMed: 19005074] [MGI Ref ID J:142399]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred together.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| 100492 B6129PF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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| phone: | 207-288-6470 |
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