Strain Name: |
B6.129P-Psen1tm1Vln/J |
|---|---|
Stock Number: |
007605 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
B6.129P-Psen1tm1Vln/J (Changed: 29-AUG-08
) |
|
B6;129P-Psen1tm1Vln/J (Changed: 07-SEP-07
) | |
| Genes & Alleles | Psen1; Psen1tm1Vln; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Fred Van Leuven, Katholieke Universiteit Leuven Strain Description
These mice possess loxP sites on either side of exon 7 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these "floxed" mice are bred to mice that express Cre recombinase, resulting offspring can have one of three resulting genotypes (only exon 7 deleted, only the neo selection cassette deleted, or both exon 7 and the neo selection cassette deleted) in the cre-expressing tissue(s). These PS1-floxed mice may be useful in generating conditional knockouts of Presenilin 1 for studying Alzheimer's Disease.Strain Development
A loxP site flanked targeting vector containing a neomycin resistance gene was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 7 of the targeted gene, and another loxP site was inserted upstream of exon 7. This construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES). Correctly targeted ES cells were injected into recipient blastocysts. The donating investigator reports that these mice were then backcrossed to C57BL/6 for 7 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred with C57BL/6J for one generation to establish the colony (see note below).NOTE: A 27 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory revealed a single loci that typed as homozygous for 129/P strain type (chromosome 12 approximately 9.8 cM) and three additional loci that were segregating for either C57BL/6J or 129/P strain type (chromosome 1 approximately 52.0 cM, chromosome 4 approximately 10.9 cM, and chromosome 8 approximately 4.4 cM). Therefore, these mice are determined to be on a mixed B6;129P genetic background (August 2008).
Related Disease (OMIM) Terms |
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Psen1tm1Vln | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Fred Van Leuven | ||
| Common Name(s) | PS1-flox; | ||
| Mutation Made By | Fred Van Leuven, Katholieke Universiteit Leuven | ||
| Gene Symbol and Name | Psen1, presenilin 1 | ||
| Chromosome | 12 | ||
| Gene Common Name(s) | AD3; Ad3h; FAD; PS-1; PS1; S182; alzheimer disease 3 homolog; presenilin-1; | ||
| Molecular Note | Exon 7 was flanked by a single loxP site in intron 6 and floxed neo cassette in intron 7. [MGI Ref ID J:87229] | ||
Control Information
| Control | ||
|---|---|---|
| 100492 B6129PF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
Genotyping Protocols
Psen1tm1Vin
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, homozygous mice may be bred together. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Psen1
004193 B6.129-Psen1tm1Mpm/J 003615 B6.129-Psen1tm1Shn/J 003822 B6;129S-Psen1tm1Shn/J View Strains carrying other alleles of Psen1 (3 strains)
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Mouse/Human Gene Homologs
Alzheimer's
Neurobiology Research
Alzheimer's Disease (Presenilin mutants)
Alzheimer's Disease
Behavioral and Learning Defects
Cre-lox System (loxP-flanked Sequences)
Research Tools
Cre-lox System (loxP-flanked Sequences)
References
Selected Reference(s)
Additional ReferencesDewachter I; Reverse D; Caluwaerts N; Ris L; Kuiperi C; Van den Haute C; Spittaels K; Umans L; Serneels L; Thiry E; Moechars D; Mercken M; Godaux E; Van Leuven F. 2002. Neuronal deficiency of presenilin 1 inhibits amyloid plaque formation and corrects hippocampal long-term potentiation but not a cognitive defect of amyloid precursor protein [V717I] transgenic mice. J Neurosci 22(9):3445-53. [PubMed: 11978821] [MGI Ref ID J:87229]
Price and Supply Information
| Strain Name: | B6.129P-Psen1tm1Vln/J |
| Stock Number: | 007605 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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