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| These transgenic mice ("SLICK" for single-neuron labeling with inducible Cre-mediated knock-out) have a tamoxifen inducible Cre-mediated recombination system and Enhanced Yellow Fluorescent Protein driven by 2 bidirectional copies of the mouse thymus cell antigen 1 promoter. When crossed with a strain containing a loxP site flanked sequence of interest. The Donating Investigator reports that founder line A mice express the transgene in small subsets of motor neurons and dorsal root ganglion neurons. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
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Description
Strain Information
Type Mutant Stock; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 18-JUN-08 Species laboratory mouse Generation N10F1 (08-JAN-09) Donating Investigator Guoping Feng, Duke University Medical Center Description
These transgenic mice ("SLICK" for single-neuron labeling with inducible Cre-mediated knock-out) have a tamoxifen inducible Cre-mediated recombination system and Enhanced Yellow Fluorescent Protein driven by 2 bidirectional copies of the mouse thymus cell antigen 1 promoter. Hemizygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make the strain homozygous. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted neuron-specific deletions only in neurons where the Thy1 promoter is active. These cells will be evident by the expression of Yellow Fluorescent Protein. Transgenic mice express YFP constitutively in neurons. The Donating Investigator reports that founder line A mice express the transgene in small subsets of motor neurons and dorsal root ganglion neurons.This strain is one of many from Dr. Guoping Feng (Duke University Medical Center) with fluorescent protein expression for neurobiological studies, including SLICK line V (Stock No. 007610), Thy1-ChR2-YFP line 18 (Stock No. 007612), and Thy1-ChR2-YFP line 9 (Stock No. 007615).
Development
A transgenic construct containing the cre/ESR1 fusion gene (CreERT2), enhanced Yellow Fluorescent Protein under the control of 2 copies (placed back to back to function bidirectionally) of the mouse thymus cell antigen 1 promoter was microinjected into C57BL/6 X SJL F1 donor eggs. Founder line A was subsequently established. The mice were backcrossed to C57BL/6 for 6 generations.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
006661 129S.B6-Tg(KRT14-RAF1/ESR1)1Pkha/J 007179 129S.Cg-Tg(UBC-cre/ESR1)1Ejb/J 005975 B6.Cg-Tg(Plp1-cre/ESR1)3Pop/J 008085 B6.Cg-Tg(UBC-cre/ESR1)1Ejb/J 009614 B6.Cg-Tg(Wfs1-cre/ERT2)2Aibs/J 008529 B6;129P-Tg(Neurog1-cre/ESR1)1Good/J 007001 B6;129S-Tg(UBC-cre/ESR1)1Ejb/J 009103 B6;C3-Tg(Wfs1-cre/ERT2)3Aibs/J 007610 B6;SJL-Tg(Thy1-cre/ESR1,-EYFP)VGfng/J 004995 C3H-Tg(Camk2a-Creb1/ESR1)3Sva/J 002712 FVB/N-Tg(PF4MER)6Kra/J 007684 STOCK Tg(Atoh1-cre/ESR1)14Fsh/J 008861 STOCK Tg(Ela1-Cre/ESR1)1Stof/J
005483 129-Tg(CAG-EYFP)7AC5Nagy/J 006148 B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J 008299 B6.Cg-Tg(NEFL-EYFP/Nefh)40Gsn/J 007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J 007911 B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J 007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J 007612 B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J 007615 B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J 005630 B6.Cg-Tg(Thy1-EYFP)15Jrs/J 003709 B6.Cg-Tg(Thy1-YFP)16Jrs/J 005627 B6.Cg-Tg(Thy1-YFP/Syp)10Jrs/J 003782 B6.Cg-Tg(Thy1-YFPH)2Jrs/J 008636 B6;C-Tg(Prnp-APP695*/EYFP)49Gsn/J 007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J 005620 B6;D2-Tg(S100B-EYFP)1Wjt/J 007610 B6;SJL-Tg(Thy1-cre/ESR1,-EYFP)VGfng/J 007880 B6SJL-Tg(Thy1-Stx1a/EYFP)1Sud/J 006618 C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J 006362 C57BL/6J-Tg(CMV-Cox8a/EYFP)17J/J 007857 C57BL/6J-Tg(Eno2-YFP/Cox8a)YRwb/J 007860 C57BL/6J-Tg(Eno2-YFP/Cox8a)ZRwb/J 009422 NOD.Cg-Tg(Itgax-EYFP)1Mnz/QtngJ 005130 STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
YFP relatedNeurobiology Research
Cre-lox System
Cre Recombinase expression in neural tissue
Fluorescent protein expression in neural tissue
Research Tools
Cre-lox System
Cre Recombinase Expression: Inducible
Fluorescent Proteins
Neurobiology Research
cell marker
cre relatedResearch Tools
Fluorescent Proteins
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Thy1-cre/ESR1,-EYFP)AGfng | ||
|---|---|---|---|
| Allele Name | transgene insertion A, Guoping Feng | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | SLICK line A; | ||
| Mutation Made By | Guoping Feng, Duke University Medical Center | ||
| Strain of Origin | (C57BL/6 x SJL)F1 | ||
| Site of Expression | tamoxifen-inducible Cre and YFP in subsets of motor neurons and dorsal root ganglion neurons, and in many neurons of the cortex, hippocampus (both CA1 and dentate granule cells) and cerebellar mossy fibers | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish | ||
| Yellow Fluorescent Protein (YFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When YFP is utilized in a transgenic construct, tissue expressing sufficient amounts of YFP will fluoresce yellowish-green when exposed to a 513 nm light source. | |||
| Expressed Gene | ESR1, estrogen receptor 1, human | ||
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory | ||
| Driver Note | Thy1 | ||
| Inducible Note | induced by tamoxifen | ||
| Molecular Note | Transgenic mice have a tamoxifen inducible Cre-mediated recombination system and Enhanced Yellow Fluorescent Protein driven by 2 bidirectional copies of the mouse thymus cell antigen 1 promoter. [MGI Ref ID J:122791] | ||
| Gene Symbol and Name | Tg(Thy1-cre/ESR1,-EYFP)AGfng, transgene insertion A, Guoping Feng | ||
| Chromosome | UN | ||
| Gene Common Name(s) | SLICK line A; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Thy1-cre/ESR1,-EYFP), QPCR
Tg(Thy1-cre/ESR1,-EYFP), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Deisseroth K; Feng G; Majewska AK; Miesenbock G; Ting A; Schnitzer MJ. 2006. Next-generation optical technologies for illuminating genetically targeted brain circuits. J Neurosci 26(41):10380-6. [PubMed: 17035522] [MGI Ref ID J:122791]
Young P; Qiu L; Wang D; Zhao S; Gross J; Feng G. 2008. Single-neuron labeling with inducible Cre-mediated knockout in transgenic mice. Nat Neurosci :. [PubMed: 18454144] [MGI Ref ID J:134019]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice may be bred as homozygotes. Mating System Homozygote x Homozygote (Female x Male) 18-JUN-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $209.90 Female or Male Homozygous for Tg(Thy1-cre/ESR1,-EYFP)AGfng
Pairs /Price (US dollars $) Pair Genotype $419.80 Homozygous for Tg(Thy1-cre/ESR1,-EYFP)AGfng x Homozygous for Tg(Thy1-cre/ESR1,-EYFP)AGfng
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $272.90 Female or Male Homozygous for Tg(Thy1-cre/ESR1,-EYFP)AGfng
Pairs /Price (US dollars $) Pair Genotype $545.80 Homozygous for Tg(Thy1-cre/ESR1,-EYFP)AGfng x Homozygous for Tg(Thy1-cre/ESR1,-EYFP)AGfng
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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