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Strain Name:

B6.129S4-StrapGt(ROSA)71Sor/J

Stock Number:

007609

Availability:

Repository- Live

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Genes & Alleles   Strap;   StrapGt(ROSA)71Sor;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Gene Trap
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Targeted Mutation
Mating System+/+ sibling x Heterozygote         (Female x Male)
Specieslaboratory mouse
Donating Investigator Philippe Soriano,   Fred Hutchinson Cancer Research Center
Generation?+F0 (07-JAN-08)

Strain Description
Mice homozygous for this mutant allele have reduced size and weight gains after birth and do not survive past 8 weeks of age. Homozygotes have an embryonic lethal phenotype, dying between E10.5 and E12.5. No gene product is detected in primary fibroblasts isolated from homozygous embryos (E9.5) by RT-PCR. Homozygous embryos have underdeveloped vasculature of the yolk sac, abnormal heart and somite development, and arrested neural tube closure and embryonic turning. Heterozygotes are viable and fertile. These Strap-mutant mice may be useful in studying cellular signaling in development and adult mice; specifically receptor tyrosine kinases (RTK; such as Ras, MAP kinase, PI3K and those in the platelet-derived growth factor (PDGF) family) and immediate early genes (IEG) induced shortly after RTK activation.

Strain Development
The retroviral gene-trap vector ROSAFARY (or reverse orientation splice acceptor for array) was designed with a promoter trap module (SAbgeo*pA; encoding a lacZ-neo fusion gene) and an frt-flanked poly-A trap module (PGKhygSD; PGK promoter-driven hygromycin gene with adenoviral splice donor). This construct was electroporated into 129S4/SvJaeSor-derived AK7.1 embryonic stem (ES) cells. ES cells with the ROSAFARY vector correctly targeted to the Strap gene were identified and used to generate mutant mice. The mice were backcrossed to the C57BL/6J background for 14 generations prior to arrival at The Jackson Laboratory. The mice were then crossed to C57BL/6J (Stock No. 000664) for one generation.

The ROSAFARY vector inserts a promoterless lacZ-neo reporter fusion gene (which functions as an artificial 3' terminal exon to intercept and terminate transcription from the targeted gene promoter) as well as a poly-A trap (which functions as an artificial 5' terminal exon to initiate transcription from the insertion site). After inserting into an intron of an endogenous gene at a permissive site and in the correct orientation, the promoter trap module and the poly-A trap module can be activated to form fusion transcripts with the 5' or 3' exons.

Mammalian Phenotype Terms assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

StrapGt(ROSA)71Sor/StrapGt(ROSA)71Sor

        involves: 129S4/SvJaeSor
  • lethality-prenatal/perinatal
  • embryonic lethality during organogenesis (MGI Ref ID J:95727)
    • homozygotes die between E10.5 and E12.5
  • cardiovascular system phenotype
  • abnormal angiogenesis (MGI Ref ID J:95727)
  • abnormal heart development (MGI Ref ID J:95727)
  • embryogenesis phenotype
  • abnormal embryo turning (MGI Ref ID J:95727)
  • abnormal somite development (MGI Ref ID J:95727)
  • nervous system phenotype
  • abnormal neural tube closure (MGI Ref ID J:95727)
    • defects in neural tube closure are seen

Gene & Allele Details

Allele Symbol StrapGt(ROSA)71Sor
Allele Name gene trapped 71, Philippe M Soriano
Mutation Made By Philippe Soriano,   Fred Hutchinson Cancer Research Center
Strain of Origin129S4/SvJaeSor
ES Cell Line NameAK7
ES Cell Line Strain129S4/SvJaeSor
Gene Symbol and Name Strap, serine/threonine kinase receptor associated protein
Chromosome 6
Gene Common Name(s) AW557906; C78091; C79202; EST C78091; MAWD; PT-WD; UNRIP; expressed sequence AW557906; expressed sequence C79202;
General Note A subline was generated in which the poly-A trap module was deleted by crossing to Gt(ROSA)26Sortm1(FLP1)Dym mice. The phenotype of this subline is indistinguishable from the parent line
Molecular Note The Rosafary gene trap vector containing a SAbetaGeo pA promoter trap module and a PGKhygSD poly-A trap module was used. [MGI Ref ID J:95727]

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Genotyping Protocols

StrapGt(ROSA)71Sor

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as heterozygotes. Homozygotes have an embryonic lethal phenotype, dying between E10.5 and E12.5.
Diet Information LabDiet® 5K52/5K67

Additional Web Information

Congenic Nomenclature

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

Developmental Biology Research
Embryonic Lethality (Homozygous)
Internal/Organ Defects (heart)
Internal/Organ Defects (multiple)
Neural Tube Defects

References

Selected Reference(s)

Chen WV; Delrow J; Corrin PD; Frazier JP; Soriano P. 2004. Identification and validation of PDGF transcriptional targets by microarray-coupled gene-trap mutagenesis. Nat Genet 36(3):304-12. [PubMed: 14981515]  [MGI Ref ID J:95727]

Price and Supply Information

Strain Name: B6.129S4-StrapGt(ROSA)71Sor/J
Stock Number: 007609

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

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- Use of MICE by companies or for-profit entities requires a license.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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