Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote         (Female x Male)   13-AUG-08 Species laboratory mouse Generation N5+F9 (05-DEC-11) Generation Definitions   Donating Investigator Glenn Radice,   Jefferson Medical College

Description
These mice possess loxP sites on either side of exon 1 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 1 deleted in the cre-expressing tissue(s).

When bred to a strain with inducible Cre recombinase expression in cardiac cells (see Stock No. 005657 for example), this mutant mouse strain may be useful in studies of myocardium physiology.

Development
A loxP site flanked targeting vector containing a FRT site flanked neo cassette was utilized in the construction of this mutant. This selection cassette was inserted into the first intron of the targeted gene, and another loxP site was inserted upstream of exon 1. This construct was electroporated into 129S6/SvEvTac derived TL1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. Resulting chimeric animals were crossed to Flp recombinase expressing mice B6;SJL-Tg(ACTFLPe)9205Dym/J (Stock No. 003800) to remove the selection cassette. The mice were then backcrossed to C57BL/6J for five generations.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Cdh2

003179

STOCK Cdh2tm1Hyn/J

View Strains carrying other alleles of Cdh2     (1 strain)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Cardiovascular Research
      Cre-lox System
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Cdh2tm1Glr
Allele Name		targeted mutation 1, Glenn L Radice
Allele Type		Targeted (Floxed/Frt)
Common Name(s)		N-cadflox;
Mutation Made By		Glenn Radice,   Jefferson Medical College
Strain of Origin		129S6/SvEvTac
ES Cell Line Name		TL1/TL-1
ES Cell Line Strain		129S6/SvEvTac
Gene Symbol and Name		Cdh2, cadherin 2
Chromosome		18
Gene Common Name(s)		CD325; CDHN; CDw325; N-cadherin; NCAD; Ncad; cadherin, N-;
Molecular Note		Exon 1, which contains the translational start site and upstream transcriptional regulatory sequences, was flanked with loxP sites. An FRT-flanked neomycin selection cassette was inserted into intron 1 and subsequently removed by crossing with mice expressing FLP recombinase in the germline. [MGI Ref ID J:98828]

Genotyping

Genotyping Information

Genotyping Protocols

Cdh2^tm1Glr, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Kostetskii I; Li J; Xiong Y; Zhou R; Ferrari VA; Patel VV; Molkentin JD; Radice GL. 2005. Induced deletion of the N-cadherin gene in the heart leads to dissolution of the intercalated disc structure. Circ Res 96(3):346-54. [PubMed: 15662031]  [MGI Ref ID J:98828]

Additional References

Cdh2^tm1Glr related

Bozdagi O; Wang XB; Nikitczuk JS; Anderson TR; Bloss EB; Radice GL; Zhou Q; Benson DL; Huntley GW. 2010. Persistence of coordinated long-term potentiation and dendritic spine enlargement at mature hippocampal CA1 synapses requires N-cadherin. J Neurosci 30(30):9984-9. [PubMed: 20668183]  [MGI Ref ID J:162856]

Cheng L; Yung A; Covarrubias M; Radice GL. 2011. Cortactin Is Required for N-cadherin Regulation of Kv1.5 Channel Function. J Biol Chem 286(23):20478-89. [PubMed: 21507952]  [MGI Ref ID J:173489]

Demireva EY; Shapiro LS; Jessell TM; Zampieri N. 2011. Motor neuron position and topographic order imposed by beta- and gamma-catenin activities. Cell 147(3):641-52. [PubMed: 22036570]  [MGI Ref ID J:178688]

Howard S; Deroo T; Fujita Y; Itasaki N. 2011. A Positive Role of Cadherin in Wnt/beta-Catenin Signalling during Epithelial-Mesenchymal Transition. PLoS One 6(8):e23899. [PubMed: 21909376]  [MGI Ref ID J:176131]

Johansson JK; Voss U; Kesavan G; Kostetskii I; Wierup N; Radice GL; Semb H. 2010. N-cadherin is dispensable for pancreas development but required for beta-cell granule turnover. Genesis 48(6):374-81. [PubMed: 20533404]  [MGI Ref ID J:163480]

Kadowaki M; Nakamura S; Machon O; Krauss S; Radice GL; Takeichi M. 2007. N-cadherin mediates cortical organization in the mouse brain. Dev Biol 304(1):22-33. [PubMed: 17222817]  [MGI Ref ID J:134436]

Lewallen KA; Shen YA; De La Torre AR; Ng BK; Meijer D; Chan JR. 2011. Assessing the role of the cadherin/catenin complex at the schwann cell-axon interface and in the initiation of myelination. J Neurosci 31(8):3032-43. [PubMed: 21414924]  [MGI Ref ID J:169864]

Li J; Levin MD; Xiong Y; Petrenko N; Patel VV; Radice GL. 2008. N-cadherin haploinsufficiency affects cardiac gap junctions and arrhythmic susceptibility. J Mol Cell Cardiol 44(3):597-606. [PubMed: 18201716]  [MGI Ref ID J:133753]

Li J; Patel VV; Kostetskii I; Xiong Y; Chu AF; Jacobson JT; Yu C; Morley GE; Molkentin JD; Radice GL. 2005. Cardiac-specific loss of N-cadherin leads to alteration in connexins with conduction slowing and arrhythmogenesis. Circ Res 97(5):474-81. [PubMed: 16100040]  [MGI Ref ID J:112334]

Luo Y; High FA; Epstein JA; Radice GL. 2006. N-cadherin is required for neural crest remodeling of the cardiac outflow tract. Dev Biol 299(2):517-28. [PubMed: 17014840]  [MGI Ref ID J:115264]

Luo Y; Radice GL. 2005. N-cadherin acts upstream of VE-cadherin in controlling vascular morphogenesis. J Cell Biol 169(1):29-34. [PubMed: 15809310]  [MGI Ref ID J:98422]

Plageman TF Jr; Zacharias AL; Gage PJ; Lang RA. 2011. Shroom3 and a Pitx2-N-cadherin pathway function cooperatively to generate asymmetric cell shape changes during gut morphogenesis. Dev Biol 357(1):227-34. [PubMed: 21726547]  [MGI Ref ID J:175443]

Pontoriero GF; Smith AN; Miller LA; Radice GL; West-Mays JA; Lang RA. 2009. Co-operative roles for E-cadherin and N-cadherin during lens vesicle separation and lens epithelial cell survival. Dev Biol 326(2):403-17. [PubMed: 18996109]  [MGI Ref ID J:145191]

Smith AN; Miller LA; Radice G; Ashery-Padan R; Lang RA. 2009. Stage-dependent modes of Pax6-Sox2 epistasis regulate lens development and eye morphogenesis. Development 136(17):2977-85. [PubMed: 19666824]  [MGI Ref ID J:158133]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as homozygotes.
Mating System	Homozygote x Homozygote         (Female x Male)   13-AUG-08
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls

General Supply Notes

• This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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