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Genes & Alleles

Cdh2;   Cdh2tm1Glr;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Donating Investigator Glenn Radice,   Jefferson Medical College Generation N5+ (03-MAR-08)

Strain Description
These mice possess loxP sites on either side of exon 1 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 1 deleted in the cre-expressing tissue(s).

When bred to a strain with inducible Cre recombinase expression in cardiac cells (see Stock No. 005657 for example), this mutant mouse strain may be useful in studies of myocardium physiology.

Strain Development
A loxP site flanked targeting vector containing a FRT site flanked neo cassette was utilized in the construction of this mutant. This selection cassette was inserted into the first intron of the targeted gene, and another loxP site was inserted upstream of exon 1. This construct was electroporated into 129S6/SvEvTac derived TL1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. Resulting chimeric animals were crossed to Flp recombinase expressing mice B6;SJL-Tg(ACTFLPe)9205Dym/J (Stock No. 003800) to remove the selection cassette. The mice were then backcrossed to C57BL/6J for 5 generations.

Gene & Allele Details

Allele Symbol		Cdh2tm1Glr
Allele Name		targeted mutation 1, Glenn L Radice
Common Name(s)		N-cadflox;
Mutation Made By		Glenn Radice,   Jefferson Medical College
Strain of Origin		129S6/SvEvTac
ES Cell Line Name		TL1/TL-1
ES Cell Line Strain		129S6/SvEvTac
Gene Symbol and Name		Cdh2, cadherin 2
Chromosome		18
Gene Common Name(s)		CD325; CDHN; CDw325; N-cadherin; NCAD; Ncad; cadherin, N-;
Molecular Note		Exon 1, which contains the translational start site and upstream transcriptional regulatory sequences, was flanked with loxP sites. An FRT-flanked neomycin selection cassette was inserted into intron 1 and subsequently removed by crossing with mice expressing FLP recombinase in the germline. [MGI Ref ID J:98828]

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Genotyping Protocols

Cdh2^tm1Glr

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as homozygotes.
Diet Information	LabDiet® 5K52/5K67

Related Strains

Strains carrying other alleles of Cdh2

003179

STOCK Cdh2tm1Hyn/J

View Strains carrying other alleles of Cdh2     (1 strain)

Additional Web Information

Congenic Nomenclature
Cre-lox or FLP-FRT Systems

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Cardiovascular Research (Cre-lox System)
Cre-lox System (loxP-flanked Sequences)
Developmental Biology Research (Cre-lox System)

References

Selected Reference(s)

Kostetskii I; Li J; Xiong Y; Zhou R; Ferrari VA; Patel VV; Molkentin JD; Radice GL. 2005. Induced deletion of the N-cadherin gene in the heart leads to dissolution of the intercalated disc structure. Circ Res 96(3):346-54. [PubMed: 15662031]  [MGI Ref ID J:98828]

Additional References

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Price and Supply Information

Strain Name:	B6.129S6(SJL)-Cdh2tm1Glr/J
Stock Number:	007611

 

This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.

Estimated Available for Sale Date: 01-SEP-08

Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.

View All Strains Under Development

Supply Details

Standard Supply	Under Development for Distribution Colony
Supply Notes	Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.
Licensing	See General Terms and Conditions below for Licensing and Use Restrictions
Control Information	View Control Information in Strain Details.

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
- Use of MICE by companies or for-profit entities requires a license.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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