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| These transgenic mice, founder line 18, express the light activated ion channel, Channelrhodopsin-2 (from the green alga Chlamydomonas reinhardtii), and Yellow Fluorescent Protein fusion gene (ChR2-YFP) under the control of the mouse thymus cell antigen 1 promoter. This mutant mouse strain may be useful for ex vivo and in vivo studies of neural activity by light stimulation, such as evoked mapping of neural connectivity. | |||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6.Cg-Tg(Thy1-ChR2/EYFP)18Gfng/J (Changed: 28-AUG-07 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Mating System Hemizygote x Noncarrier (Female x Male) Species laboratory mouse Generation N7+ (01-NOV-07) Donating Investigator Guoping Feng, Duke University Medical Center Description
These founder line 18 transgenic mice express the light-activated ion channel, Channelrhodopsin-2 (from the green alga Chlamydomonas reinhardtii), fused to Yellow Fluorescent Protein (ChR2-YFP) under the control of the mouse thymus cell antigen 1 (Thy1) promoter. Hemizygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.Expression of the transgenic ChR2-YFP fusion protein is detected in layer 5 cortical neurons, CA1 and CA3 pyramidal neurons of the hippocampus, cerebellar mossy fibers, neurons in the thalamus, midbrain and brainstem, and the olfactory bulb mitral cells. Neurons expressing the transgene are morphologically and physiologically comparable to non-mutant neurons. This mutant mouse strain may be useful for ex vivo and in vivo neural circuitry mapping studies using light stimulation.
This strain is one of many from Dr. Guoping Feng (Duke University Medical Center) with fluorescent protein expression for neurobiological studies, including SLICK line A (Stock No. 007606), SLICK line V (Stock No. 007610), and Thy1-ChR2-YFP line 9 (Stock No. 007615).
View YFP fluorescence in sagittal brain sections for this strain.
Development
A transgenic construct containing Channelrhodopsin-2 from the green alga Chlamydomonas reinhardtii and Yellow Fluorescent Protein fusion gene under the control of the mouse thymus cell antigen 1 promoter was injected into C57BL/6 x SJL F1 hybrid donor eggs. Founder line 18 was subsequently established. The mice were backcrossed to C57BL/6 for 6 generations before arriving at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (169 strains)
007615 B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J
005483 129-Tg(CAG-EYFP)7AC5Nagy/J 006148 B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J 007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J 007911 B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J 007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J 007615 B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J 005630 B6.Cg-Tg(Thy1-EYFP)15Jrs/J 003709 B6.Cg-Tg(Thy1-YFP)16Jrs/J 005627 B6.Cg-Tg(Thy1-YFP/Syp)10Jrs/J 003782 B6.Cg-Tg(Thy1-YFPH)2Jrs/J 007606 B6.Cg-Tg(Thy1-cre/ESR1,-EYFP)AGfng/J 007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J 005620 B6;D2-Tg(S100B-EYFP)1Wjt/J 007610 B6;SJL-Tg(Thy1-cre/ESR1,-EYFP)VGfng/J 007880 B6SJL-Tg(Thy1-Stx1a/EYFP)1Sud/J 006618 C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J 006362 C57BL/6J-Tg(CMV-Cox8a/EYFP)17J/J 007860 C57BL/6J-Tg(Eno2-YFP/Cox8a)ZRwb/J 005130 STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Thy1-COP4/EYFP)18Gfng/0
involves: C57BL/6 * SJL
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:122790)
- neurons expressing EYFP show no signs of degeneration or altered morphology
This mouse can be used to support research in many areas including:
YFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
Neurobiology Research (cell marker)
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Thy1-COP4/EYFP)18Gfng | ||
|---|---|---|---|
| Allele Name | transgene insertion 18, Guoping Feng | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | ChR2-YFP; Thy1-ChR2-YFP; | ||
| Strain of Origin | C57BL/6 x SJL | ||
| Site of Expression | layer 5 cortical neurons, CA1 and CA3 pyramidal neurons of the hippocampus, cerebellar mossy fibers, neurons in the thalamus, midbrain and brainstem, and mitral cells of the olfactory bulb; channelrhodopsin-2 and YFP | ||
| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish | ||
| Yellow Fluorescent Protein (YFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When YFP is utilized in a transgenic construct, tissue expressing sufficient amounts of YFP will fluoresce yellowish-green when exposed to a 513 nm light source. | |||
| Expressed Gene | COP4, Chlamydomonas reinhardtii, | ||
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory | ||
| General Note | Lines initiated from 7 of 10 transgenic founder mice exhibited expression of the chimeric protein in the central and/or peripheral nervous system, the specific pattern of expression varying among lines. Line 18 was selected for use in experiments. (J:122790) | ||
| Molecular Note |
This transgene gives rise to a fusion protein composed of the first 315 amino acids of the light-activated, non-specific ion channel chlamyopsin 4 (COP4), also known as channelopsin 2 (CHOP2) and as channelrhodopsin 2 (ChR2), from the green alga Chlamydomonas reinhardtii joined to enhanced yellow fluorescent protein. The chimeric cDNA has replaced the Thy1 coding sequence and its associated introns, which contain sequences directing expression in non-neuronal cell types, in a mouse Thy1-derived expression vector that retains the essential promoter, neuron-specific upstream regulatory sequences, and downstream sequences including the polyadenylation signal. Immunohistochemical analysis shows that mice with this transgene express the chimeric protein in neurons of several brain regions, including various nuclei of the brainstem, midbrain and thalamus; mossy fibers in the cerebellum; CA1 and CA2 pyramidal neurons of the hippocampus; and strongly in layer 5 cortical neurons. In the olfactory system, mice of this line express COP4/EYFP on membranes of the majority of mitral cells and in the neuropil of the inner plexiform layer of the olfactory bulb, where colocalization with the 5-H2A receptor, but not with GAD67, calbindin or PAX6, demonstrates that expression is specific to mitral cells. [MGI Ref ID J:122790] [MGI Ref ID J:123609] [MGI Ref ID J:123610] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Thy1-COP4/EYFP)18Gfng, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Arenkiel BR; Peca J; Davison IG; Feliciano C; Deisseroth K; Augustine GJ; Ehlers MD; Feng G. 2007. In vivo light-induced activation of neural circuitry in transgenic mice expressing channelrhodopsin-2. Neuron 54(2):205-18. [PubMed: 17442243] [MGI Ref ID J:122790]
Additional References
Tg(Thy1-COP4/EYFP)18Gfng relatedBoyden ES; Zhang F; Bamberg E; Nagel G; Deisseroth K. 2005. Millisecond-timescale, genetically targeted optical control of neural activity. Nat Neurosci 8(9):1263-8. [PubMed: 16116447] [MGI Ref ID J:123609]
Caroni P. 1997. Overexpression of growth-associated proteins in the neurons of adult transgenic mice. J Neurosci Methods 71(1):3-9. [PubMed: 9125370] [MGI Ref ID J:123610]
Wang H; Peca J; Matsuzaki M; Matsuzaki K; Noguchi J; Qiu L; Wang D; Zhang F; Boyden E; Deisseroth K; Kasai H; Hall WC; Feng G; Augustine GJ. 2007. High-speed mapping of synaptic connectivity using photostimulation in Channelrhodopsin-2 transgenic mice. Proc Natl Acad Sci U S A 104(19):8143-8. [PubMed: 17483470] [MGI Ref ID J:122789]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as hemizygotes. The Donating Investigator has not attempted to make the strain homozygous. Mating System Hemizygote x Noncarrier (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $236.40 Female or Male Hemizygous for Tg(Thy1-COP4/EYFP)18Gfng *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $288.65 Hemizygous for Tg(Thy1-COP4/EYFP)18Gfng x Noncarrier $288.65 Noncarrier x Hemizygous for Tg(Thy1-COP4/EYFP)18Gfng
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $307.40 Female or Male Hemizygous for Tg(Thy1-COP4/EYFP)18Gfng *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $375.30 Hemizygous for Tg(Thy1-COP4/EYFP)18Gfng x Noncarrier $375.30 Noncarrier x Hemizygous for Tg(Thy1-COP4/EYFP)18Gfng
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
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