Strain Name: |
B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J |
|---|---|
Stock Number: |
007612 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
B6.Cg-Tg(Thy1-ChR2/EYFP)18Gfng/J (Changed: 28-AUG-07
) |
| Genes & Alleles | COP4; Thy1; YFP; Tg(Thy1-COP4/EYFP)18Gfng; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Hemizygote x Noncarrier (Female x Male) Species laboratory mouse Donating Investigator Guoping Feng, Duke University Medical Center Generation N7+ (01-NOV-07) Strain Description
These founder line 18 transgenic mice express the light-activated ion channel, Channelrhodopsin-2 (from the green alga Chlamydomonas reinhardtii), fused to Yellow Fluorescent Protein (ChR2-YFP) under the control of the mouse thymus cell antigen 1 (Thy1) promoter. Hemizygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.Expression of the transgenic ChR2-YFP fusion protein is detected in layer 5 cortical neurons, CA1 and CA3 pyramidal neurons of the hippocampus, cerebellar mossy fibers, neurons in the thalamus, midbrain and brainstem, and the olfactory bulb mitral cells. Neurons expressing the transgene are morphologically and physiologically comparable to non-mutant neurons. This mutant mouse strain may be useful for ex vivo and in vivo neural circuitry mapping studies using light stimulation.
Strain Development
A transgenic construct containing Channelrhodopsin-2 from the green alga Chlamydomonas reinhardtii and Yellow Fluorescent Protein fusion gene under the control of the mouse thymus cell antigen 1 promoter was injected into C57BL/6 x SJL F1 hybrid donor eggs. Founder line 18 was subsequently established. The mice were backcrossed to C57BL/6 for 6 generations before arriving at The Jackson Laboratory.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Thy1-COP4/EYFP)18Gfng | ||
|---|---|---|---|
| Allele Name | transgene insertion 18, Guoping Feng | ||
| Common Name(s) | ChR2-YFP; Thy1-ChR2-YFP; | ||
| Strain of Origin | C57BL/6 x SJL | ||
| Site of Expression | layer 5 cortical neurons, CA1 and CA3 pyramidal neurons of the hippocampus, cerebellar mossy fibers, neurons in the thalamus, midbrain and brainstem, and mitral cells of the olfactory bulb; channelrhodopsin-2 and YFP | ||
| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish | ||
| Yellow Fluorescent Protein (YFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When YFP is utilized in a transgenic construct, tissue expressing sufficient amounts of YFP will fluoresce yellowish-green when exposed to a 513 nm light source. | |||
| Expressed Gene | COP4, Chlamydomonas reinhardtii, | ||
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory | ||
| General Note | Lines initiated from 7 of 10 transgenic founder mice exhibited expression of the chimeric protein in the central and/or peripheral nervous system, the specific pattern of expression varying among lines. Line 18 was selected for use in experiments. (J:122790) | ||
| Molecular Note |
This transgene gives rise to a fusion protein composed of the first 315 amino acids of the light-activated, non-specific ion channel chlamyopsin 4 (COP4), also known as channelopsin 2 (CHOP2) and as channelrhodopsin 2 (ChR2), from the green alga Chlamydomonas reinhardtii joined to enhanced yellow fluorescent protein. The chimeric cDNA has replaced the Thy1 coding sequence and its associated introns, which contain sequences directing expression in non-neuronal cell types, in a mouse Thy1-derived expression vector that retains the essential promoter, neuron-specific upstream regulatory sequences, and downstream sequences including the polyadenylation signal. Immunohistochemical analysis shows that mice with this transgene express the chimeric protein in neurons of several brain regions, including various nuclei of the brainstem, midbrain and thalamus; mossy fibers in the cerebellum; CA1 and CA2 pyramidal neurons of the hippocampus; and strongly in layer 5 cortical neurons. In the olfactory system, mice of this line express COP4/EYFP on membranes of the majority of mitral cells and in the neuropil of the inner plexiform layer of the olfactory bulb, where colocalization with the 5-H2A receptor, but not with GAD67, calbindin or PAX6, demonstrates that expression is specific to mitral cells. [J:122790] [J:123609] [J:123610] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as hemizygotes. The Donating Investigator has not attempted to make the strain homozygous. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
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Fluorescent Protein Strains
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007615 B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J
005483 129-Tg(CAG-EYFP)7AC5Nagy/J 006148 B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J 007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J 007911 B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J 007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J 007615 B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J 005630 B6.Cg-Tg(Thy1-EYFP)15Jrs/J 003709 B6.Cg-Tg(Thy1-YFP)16Jrs/J 005627 B6.Cg-Tg(Thy1-YFP/Syp)10Jrs/J 003782 B6.Cg-Tg(Thy1-YFPH)2Jrs/J 007606 B6.Cg-Tg(Thy1-cre/ESR1,-EYFP)AGfng/J 007610 B6.Cg-Tg(Thy1-cre/ESR1,-EYFP)VGfng/J 007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J 005620 B6;D2-Tg(S100B-EYFP)1Wjt/J 007880 B6SJL-Tg(Thy1-Stx1a/EYFP)1Sud/J 006618 C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J 006362 C57BL/6J-Tg(CMV-Cox8a/EYFP)17J/J 005130 STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
YFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
Neurobiology Research (cell marker)
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Additional ReferencesArenkiel BR; Peca J; Davison IG; Feliciano C; Deisseroth K; Augustine GJ; Ehlers MD; Feng G. 2007. In vivo light-induced activation of neural circuitry in transgenic mice expressing channelrhodopsin-2. Neuron 54(2):205-18. [PubMed: 17442243] [J:122790]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J |
| Stock Number: | 007612 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. No age specifications accepted. Colony sized to produce minimal quantities (typically up to 6 mice) upon order receipt; one order per strain. Expected delivery: 1-3 months. Larger quantities or custom orders arranged upon request. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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