Strain Name: |
B6;129-Smad1tm2Sor/J |
|---|---|
Stock Number: |
007613 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Smad1; Smad1tm2Sor; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System Heterozygote x Heterozygote (Female x Male) Species laboratory mouse Donating Investigator Philippe Soriano, Fred Hutchinson Cancer Research Center Strain Description
Homozygotes for the Smad1tm2Sor (also called Smad1L) allele are viable and fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice carry a mutation in exon 3, which effects MAPK-mediated phosphorylation of the protein. Western blot analysis of MEFs from homozygotes showed that similar protein levels compared to wildtype. Homozygous embryos have fewer primordial germ cells than wildtype controls. Homozygous mice display abnormal gastric mucosa cell population ratios with fewer zymogenic cells and more parietal cells. The cytoskeleton of MEFs from homozygotes exhibit a loss of adhesion zippers, decreased stress fibers, and an accumulation of actin in the cortical regions with an increase in beta-catenin immunostaining localized to the cell membranes. This mutant mouse strain may be useful in studies of stomach development, gastic mucosal homeostasis and BMP and MAPK signaling pathways during development and in the adult.Strain Development
A targeting vector containing a floxed PGKneo cassette and a diptheria toxin cassette was used to introduce serine-to-alanine substitutions in the MAPK-consensus phosphorylation sites (S187A, S195A, S206A, S214A) and two conserved phosphoserines in exon 3 (S209 and S210). The construct was electroporated into 129S4/SvJaeSor-derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The mice were crossed to a strain expressing Cre under the control of the Meox2 promoter to remove the floxed PGKneo cassette. The mice were then crossed to mice carrying the Smad1tm1Sor mutant allele prior to arriving at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J to separate the mutant alleles. These mice carry only the Smad1tm2Sor (also called Smad1L) mutant allele.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Smad1tm2Sor | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Philippe Soriano | ||
| Common Name(s) | Smad1L; | ||
| Mutation Made By | Philippe Soriano, Fred Hutchinson Cancer Research Center | ||
| Strain of Origin | 129S4/SvJaeSor | ||
| ES Cell Line Name | AK7 | ||
| ES Cell Line Strain | 129S4/SvJaeSor | ||
| Gene Symbol and Name | Smad1, MAD homolog 1 (Drosophila) | ||
| Chromosome | 8 | ||
| Gene Common Name(s) | AI528653; BSP1; JV4-1; JV41; MADH1; MADR1; Madh1; Smad 1; expressed sequence AI528653; | ||
| Molecular Note | Homologous recombination incorporated serine-to-alanine substitutions in the MAPK-consensus phosphorylation sites (S187A, S195A, S206A, S214A) and two conserved phosphoserines in exon 3 (S209 and S210). Western blot analysis of MEF nuclear extracts demonstrated the mutant protein was expressed at similar levels as that of wild type. [MGI Ref ID J:90769] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice can be bred as homozygotes. |
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| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Smad1
007608 B6;129-Smad1tm1Sor/J View Strains carrying other alleles of Smad1 (1 strain)
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Cell Biology Research
Signal Transduction
Developmental Biology Research
Defects in Cell Adhesion Molecules
Internal/Organ Research
Gastrointestinal Defects
Reproductive Biology Research
Developmental Defects Affecting Gonads (germ cell deficient)
References
Selected Reference(s)
Aubin J; Davy A; Soriano P. 2004. In vivo convergence of BMP and MAPK signaling pathways: impact of differential Smad1 phosphorylation on development and homeostasis. Genes Dev 18(12):1482-94. [PubMed: 15198985] [MGI Ref ID J:90769]
Price and Supply Information
| Strain Name: | B6;129-Smad1tm2Sor/J |
| Stock Number: | 007613 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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