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| These transgenic mice, founder line 9, express the light activated ion channel, Channelrhodopsin-2 (from the green alga Chlamydomonas reinhardtii), and Yellow Fluorescent Protein fusion gene (ChR2-YFP) under the control of the mouse thymus cell antigen 1 promoter. This mutant mouse strain may be useful for ex vivo and in vivo studies of neural circuitry by light stimulation, such as evoked mapping of neural connectivity. | |||||||||||||||
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Description
Strain Information
Former Names B6.Cg-Tg(Thy1-ChR2/EYFP)9Gfng/J (Changed: 28-AUG-07 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 13-AUG-08 Species laboratory mouse Generation N11F1 (12-NOV-12)
Generation DefinitionsDonating Investigator Guoping Feng, Massachusetts Institute of Technology Description
These Thy1-ChR2-YFP founder line 9 transgenic mice express the light-activated ion channel, Channelrhodopsin-2 (from the green alga Chlamydomonas reinhardtii), fused to Yellow Fluorescent Protein (ChR2-YFP) under the control of the mouse thymus cell antigen 1 (Thy1) promoter. Hemizygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Expression of the transgenic ChR2-YFP fusion protein is detected throughout the brain, including in the cortex, hippocampus, thalamus, midbrain, brainstem, cerebellar mossy fibers and retinal ganglion cells. Neurons expressing the transgene are morphologically and physiologically comparable to non-mutant neurons. This mutant mouse strain may be useful for ex vivo and in vivo neural circuitry mapping studies using light stimulation.The ChR2-YFP fusion protein is composed of a Chlamydomonas reinhardtii-derived channelrhodopsin-2 (ChR2) fused in-frame with an enhanced yellow fluorescent protein (EYFP).
The bacterial opsins are retinal-binding proteins that combine a light-sensitive domain with an ion channel or pump; providing light-dependent ion transport, membrane potential alteration, and sensory functions to bacteria. ChR2 functions as a blue light-driven cation channel that depolarizes the cell and causes action potentials. As such, illuminating ChR2-expressing neurons with blue light (~470 nm) leads to rapid and reversible photostimulation of action potential firing/neural activity in these cells.
This optogenetic strain is one of many from the same transgene creator/donating investigator with light-inducible neurobiology applications; including Thy1-ChR2-YFP line 18 (Stock No. 007612), Thy1-eNpHR-YFP line 2 (Stock No. 012332), Thy1-eNpHR-YFP line 4 (Stock No. 012334), Thy1-vChR1-YFP line 1 (Stock No. 012341), Thy1-vChR1-YFP line 4 (Stock No. 012344), Thy1-vChR1-YFP line 8 (Stock No. 012348), Thy1-mhChR2-YFP Line 20 (Stock No. 012350), Prv-mhChR2-YFP Line 15 (Stock No. 012355), ChAT-ChR2-YFP line 5 (Stock No. 014545), ChAT-ChR2-YFP line 6 (Stock No. 014546), VGAT-ChR2-YFP line 8 (Stock No. 014548), and TpH2-ChR2-YFP line 5 (Stock No. 014555).
View YFP fluorescence in sagittal brain sections for this strain.
Development
A transgenic construct containing Channelrhodopsin-2 from the green alga Chlamydomonas reinhardtii and Yellow Fluorescent Protein fusion gene under the control of the mouse thymus cell antigen 1 promoter was injected into C57BL/6 X SJL F1 hybrid donor eggs. Founder line 9 was subsequently established. The mice were backcrossed to C57BL/6 for six generations before arriving at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
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Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Thy1-COP4/EYFP)9Gfng/0
involves: C57BL/6 * SJL
- normal phenotype
- no abnormal phenotype detected
- neurons expressing EYFP show no signs of degeneration or altered morphology (MGI Ref ID J:122790)
This mouse can be used to support research in many areas including:
YFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Optogenetic and Pharmacogenetic tools
Channelrhodopsin expressing strains
Research Tools
Fluorescent Proteins
Neurobiology Research
cell marker
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Thy1-COP4/EYFP)9Gfng | ||
|---|---|---|---|
| Allele Name | transgene insertion 9, Guoping Feng | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | ChR2-YFP; Thy1-ChR2-YFP; | ||
| Strain of Origin | C57BL/6 x SJL | ||
| Site of Expression | widespread expression in the brain including the cortex, hippocampus, thalamus, midbrain, brainstem, cerebellar mossy fibers amd retinal ganglion cells; channelrhodopsin-2 and YFP | ||
| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish | ||
| Yellow Fluorescent Protein (YFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When YFP is utilized in a transgenic construct, tissue expressing sufficient amounts of YFP will fluoresce yellowish-green when exposed to a 513 nm light source. | |||
| Expressed Gene | COP4, Channelrhodopsin, Chlamydomonas | ||
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory | ||
| General Note | Lines initiated from 7 of 10 transgenic founder mice exhibited expression of the chimeric protein in the central and/or peripheral nervous system, the specific pattern of expression varying among lines. | ||
| Molecular Note |
This transgene gives rise to a fusion protein composed of the first 315 amino acids of the light-activated, non-specific ion channel chlamyopsin 4 (COP4), also known as channelopsin 2 (CHOP2) and as channelrhodopsin 2 (ChR2), from the green alga Chlamydomonas reinhardtii joined to enhanced yellow fluorescent protein. The chimeric cDNA has replaced the Thy1 coding sequence and its associated introns, which contain sequences directing expression in non-neuronal cell types, in a mouse Thy1-derived expression vector that retains the essential promoter, neuron-specific upstream regulatory sequences, and downstream sequences including the polyadenylation signal. Immunohistochemical analysis shows that mice with this transgene express the chimeric protein in neurons of several brain regions, including various nuclei of the brainstem, midbrain and thalamus; CA1 and CA3 pyramidal neurons of the hippocampus; strongly in layer 5 neurons of the cortex; and in retinal ganglion cells. [MGI Ref ID J:122790] | ||
| Gene Symbol and Name | Tg(Thy1-COP4/EYFP)9Gfng, transgene insertion 9, Guoping Feng | ||
| Chromosome | UN | ||
| Gene Common Name(s) | ChR2-YFP; Thy1-ChR2-YFP; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Melt Curve Analysis
Fluorescent Proteins (Generic GFP), Standard PCR
Fluorescent Proteins -- Generic GFP, QPCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Arenkiel BR; Peca J; Davison IG; Feliciano C; Deisseroth K; Augustine GJ; Ehlers MD; Feng G. 2007. In vivo light-induced activation of neural circuitry in transgenic mice expressing channelrhodopsin-2. Neuron 54(2):205-18. [PubMed: 17442243] [MGI Ref ID J:122790]
Additional References
Tg(Thy1-COP4/EYFP)9Gfng relatedThyagarajan S; van Wyk M; Lehmann K; Lowel S; Feng G; Wassle H. 2010. Visual function in mice with photoreceptor degeneration and transgenic expression of channelrhodopsin 2 in ganglion cells. J Neurosci 30(26):8745-58. [PubMed: 20592196] [MGI Ref ID J:161847]
Wang H; Peca J; Matsuzaki M; Matsuzaki K; Noguchi J; Qiu L; Wang D; Zhang F; Boyden E; Deisseroth K; Kasai H; Hall WC; Feng G; Augustine GJ. 2007. High-speed mapping of synaptic connectivity using photostimulation in Channelrhodopsin-2 transgenic mice. Proc Natl Acad Sci U S A 104(19):8143-8. [PubMed: 17483470] [MGI Ref ID J:122789]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as hemizygotes. The Donating Investigator has not attempted to make the strain homozygous. Mating System Noncarrier x Hemizygote (Female x Male) 13-AUG-08 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(Thy1-COP4/EYFP)9Gfng
Price per Pair (US dollars $) Pair Genotype $296.00 Hemizygous for Tg(Thy1-COP4/EYFP)9Gfng x Noncarrier $296.00 Noncarrier x Hemizygous for Tg(Thy1-COP4/EYFP)9Gfng Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(Thy1-COP4/EYFP)9Gfng
Price per Pair (US dollars $) Pair Genotype $384.80 Hemizygous for Tg(Thy1-COP4/EYFP)9Gfng x Noncarrier $384.80 Noncarrier x Hemizygous for Tg(Thy1-COP4/EYFP)9Gfng Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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