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| These transgenic mice express a hemaglutinin-tagged copy of the Arntl (Bmal1) gene under the control of a tetracycline-responsive promoter element (TRE or tetO). When bred with another transgenic mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), transgene expression can be regulated with the tetracycline analog, doxycycline. This strain may be useful in studies dilineating the mechanism of mammalian circadian rhythms. | |||||||||||||||
- Description
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation ?pN1 Donating Investigator Joseph Takahashi, Northwestern University Description
Hemizygous mice carrying this mutation are viable, fertile, and display no apparent defects or abnormalities. These transgenic mice express a hemaglutinin-tagged copy of the Arntl (Bmal1) gene under the control of a tetracycline-responsive promoter element (TRE or tetO). When bred with another transgenic mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA) to create a bitransgenic animal, transgene expression can be regulated with the tetracycline analog, doxycycline. This strain may be useful in studies dilineating the mechanism of mammalian circadian rhythms.Development
A transgenic construct incorporating a hemagglutinin (HA) tag in the C-terminus of the mouse Arnt1 (Bmal1) gene downstream of a tetracyline operator (tetO) sequence was introduced to a CD1 outbred mouse background. This line was backcrossed to C57BL/6 for ten generations by the donating laboratory.
Related Strains
Strains carrying other alleles of Arntl
009100 B6.129-Arntltm1Bra/J 007668 B6.129S4(Cg)-Arntltm1Weit/J View Strains carrying other alleles of Arntl (2 strains)
Additional Web Information
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Neurobiology Research
Circadian Rhythms
Research Tools
Tet Expression Systems
tTA/rtTA Responsive Strains
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(tetO-Arntl)1Jt | ||
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| Allele Name | transgene insertion 1, Joseph S Takahashi | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | tetO::Bmal1-HA; | ||
| Strain of Origin | CD-1 | ||
| Expressed Gene | Arntl, aryl hydrocarbon receptor nuclear translocator-like, mouse, laboratory | ||
| Promoter | tetO, tet operator, | ||
| Molecular Note | A 2.1 kb fragment containing hemagglutinin (HA)-tagged Arnt1 cDNA was inserted into a vector downstream of the tetracycline operator (tetO) sequence. The construct was injected into fertilized CD-1 oocytes. [MGI Ref ID J:116189] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(tetO-Arntl)1Jt, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
McDearmon EL; Patel KN; Ko CH; Walisser JA; Schook AC; Chong JL; Wilsbacher LD; Song EJ; Hong HK; Bradfield CA; Takahashi JS. 2006. Dissecting the functions of the mammalian clock protein BMAL1 by tissue-specific rescue in mice. Science 314(5803):1304-8. [PubMed: 17124323] [MGI Ref ID J:116189]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintained as a live colony, hemizygotes may be used for breeding.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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