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| These transgenic mice express a hemaglutinin-tagged copy of the Arntl (Bmal1) gene under the control of a tetracycline-responsive promoter element (TRE or tetO). When bred with another transgenic mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), transgene expression can be regulated with the tetracycline analog, doxycycline. This strain may be useful in studies dilineating the mechanism of mammalian circadian rhythms. | |||||||||||||||
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation ?pN1 Donating Investigator Joseph Takahashi, Northwestern University Description
Hemizygous mice carrying this mutation are viable, fertile, and display no apparent defects or abnormalities. These transgenic mice express a hemaglutinin-tagged copy of the Arntl (Bmal1) gene under the control of a tetracycline-responsive promoter element (TRE or tetO). When bred with another transgenic mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA) to create a bitransgenic animal, transgene expression can be regulated with the tetracycline analog, doxycycline. This strain may be useful in studies dilineating the mechanism of mammalian circadian rhythms.Development
A transgenic construct incorporating a hemagglutinin (HA) tag in the C-terminus of the mouse Arnt1 (Bmal1) gene downstream of a tetracyline operator (tetO) sequence was introduced to a CD1 outbred mouse background. This line was backcrossed to C57BL/6 for ten generations by the donating laboratory.
Strains carrying other alleles of Arntl
009100 B6.129-Arntltm1Bra/J 007668 B6.129S4(Cg)-Arntltm1Weit/J View Strains carrying other alleles of Arntl (2 strains)
Strains carrying other alleles of tetO
View Strains carrying other alleles of tetO (36 strains)
Tet Expression Systems
View Research Applications
Research Applications
This mouse can be used to support research in many areas including:
Neurobiology Research
Circadian Rhythms
Research Tools
Tet Expression Systems
tTA/rtTA Responsive Strains
| Allele Symbol | Tg(tetO-Arntl)1Jt | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Joseph S Takahashi | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | tetO::Bmal1-HA; | ||
| Strain of Origin | CD-1 | ||
| Expressed Gene | Arntl, aryl hydrocarbon receptor nuclear translocator-like, mouse, laboratory | ||
| Promoter | tetO, tet operator, | ||
| Molecular Note | A 2.1 kb fragment containing hemagglutinin (HA)-tagged Arnt1 cDNA was inserted into a vector downstream of the tetracycline operator (tetO) sequence. The construct was injected into fertilized CD-1 oocytes. [MGI Ref ID J:116189] | ||
Genotyping Protocols
Tg(tetO-Arntl)1Jt, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
McDearmon EL; Patel KN; Ko CH; Walisser JA; Schook AC; Chong JL; Wilsbacher LD; Song EJ; Hong HK; Bradfield CA; Takahashi JS. 2006. Dissecting the functions of the mammalian clock protein BMAL1 by tissue-specific rescue in mice. Science 314(5803):1304-8. [PubMed: 17124323] [MGI Ref ID J:116189]
Colony Maintenance
Breeding & Husbandry When maintained as a live colony, hemizygotes may be used for breeding.
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
| Pricing for International shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Purchasing Information
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Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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