Strain Name: |
B6.Cg-Tg(tetO-Arntl)1Jt/J |
|---|---|
Stock Number: |
007618 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Arntl; Tg(tetO-Arntl)1Jt; tetO; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Joseph Takahashi, Northwestern University Generation ?pN1 Strain Description
Hemizygous mice carrying this mutation are viable, fertile, and display no apparent defects or abnormalities. These transgenic mice express a hemaglutinin-tagged copy of the Arntl (Bmal1) gene under the control of a tetracycline-responsive promoter element (TRE or tetO). When bred with another transgenic mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA) to create a bitransgenic animal, transgene expression can be regulated with the tetracycline analog, doxycycline. This strain may be useful in studies dilineating the mechanism of mammalian circadian rhythms.Strain Development
A transgenic construct incorporating a hemagglutinin (HA) tag in the C-terminus of the mouse Arnt1 (Bmal1) gene downstream of a tetracyline operator (tetO) sequence was introduced to a CD1 outbred mouse background. This line was backcrossed to C57BL/6 for ten generations by the donating laboratory.
Gene & Allele Details
| Allele Symbol | Tg(tetO-Arntl)1Jt | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Joseph S Takahashi | ||
| Common Name(s) | tetO::Bmal1-HA; | ||
| Strain of Origin | CD-1 | ||
| Expressed Gene | Arntl, aryl hydrocarbon receptor nuclear translocator-like, mouse, laboratory | ||
| Promoter | tetO, tet operator, | ||
| Molecular Note | A 2.1 kb fragment containing hemagglutinin (HA)-tagged Arnt1 cDNA was inserted into a vector downstream of the tetracycline operator (tetO) sequence. The construct was injected into fertilized CD-1 oocytes. [MGI Ref ID J:116189] | ||
Genotyping Protocols
Tg(tetO-Arntl)1Jt
Colony Maintenance
| Breeding & Husbandry | When maintained as a live colony, hemizygotes may be used for breeding. |
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Related Strains
Strains carrying other alleles of Arntl
007668 B6.129S4(Cg)-Arntltm1Weit/J View Strains carrying other alleles of Arntl (1 strain)
Additional Web Information
Congenic Nomenclature
Tet Expression Systems
Research Applications
This mouse can be used to support research in many areas including:
Neurobiology Research
Circadian Rhythms
Research Tools
Tet Expression Systems (tTA/rtTA Responsive Strains)
References
Selected Reference(s)
McDearmon EL; Patel KN; Ko CH; Walisser JA; Schook AC; Chong JL; Wilsbacher LD; Song EJ; Hong HK; Bradfield CA; Takahashi JS. 2006. Dissecting the functions of the mammalian clock protein BMAL1 by tissue-specific rescue in mice. Science 314(5803):1304-8. [PubMed: 17124323] [MGI Ref ID J:116189]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(tetO-Arntl)1Jt/J |
| Stock Number: | 007618 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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