Strain Name:

B6.129P2-Mrc1tm1Mnz/J

Stock Number:

007620

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-Mice are defective in clearing proteins that bear accessible mannose and N-acetylglucosamine residues and have elevated levels of eight different lysosomal hydrolases. Clearance of a subset of mannose-bearing serum glycoproteins that are normally elevated during inflammation is impaired. This strain may be useful in studies of serum glycoprotein homeostasis.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationN?pN1
Generation Definitions
 
Donating Investigator Michel Nussenzweig,   The Rockefeller University

Description
Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. Mice are defective in clearing proteins that bear accessible mannose and N-acetylglucosamine residues and have elevated levels of eight different lysosomal hydrolases. Clearance of a subset of mannose-bearing serum glycoproteins that are normally elevated during inflammation is impaired. Expression is abolished as shown by immunoblot of lymph node lysates. EGFP expression has not been assessed. This strain may be useful in studies of serum glycoprotein homeostasis.

Development
A targeting vector was designed to insert an enhanced green fluorescence protein cassette (EGFP) and stop codon into the exon 1 start codon of the gene. EGFP was followed by a self-excising floxed Ace-Cre (angiotensin I converting enzyme promoter)/neomycin resistance cassette flanked by loxP sites. The targeting vector was introduced to 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. The Cre/Neo element was removed when passed through the chimeric male germline. This line was backcrossed to C57BL/6 eight times by the donating laboratory.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Mrc1tm1Mnz/Mrc1tm1Mnz

        B6.129P2-Mrc1tm1Mnz
  • immune system phenotype
  • decreased T cell number
    • Mesocestoides corti infected mutants show diminished numbers of T cells in the brains compared to infected wild-type controls   (MGI Ref ID J:194065)
  • decreased susceptibility to parasitic infection
    • mutants show decreased susceptibility to infection with the parasite Mesocestoides corti, showing increased survival times after infection and decreased disease severity   (MGI Ref ID J:194065)
    • mutants exhibit lower levels of IL-1beta, CCL5 and IL-6 cytokines following Mesocestoides corti infection   (MGI Ref ID J:194065)
    • the immune cell subsets that infilatrate the brain following Mesocestoides croti infection are altered and characterized by reduced numbers of T cells and accumulation of granulocytic cells   (MGI Ref ID J:194065)
    • decreased susceptibility to parasitic infection induced morbidity/mortality
      • mutants show increased survival times after infection with the parasite Mesocestoides corti compared to wild-type mice   (MGI Ref ID J:194065)
  • increased granulocyte number
    • mice show a decrease in the numbers of dendritic cells and monocytes/macrophages and an increased accumulation of granulocytic myeloid cells after Mesocestoides corti infection compared with wild-type mice   (MGI Ref ID J:194065)
    • granulocytes from Mesocestoides corti infected mutants exhibit a regulatory phenotype   (MGI Ref ID J:194065)
  • mortality/aging
  • decreased susceptibility to parasitic infection induced morbidity/mortality
    • mutants show increased survival times after infection with the parasite Mesocestoides corti compared to wild-type mice   (MGI Ref ID J:194065)
  • hematopoietic system phenotype
  • decreased T cell number
    • Mesocestoides corti infected mutants show diminished numbers of T cells in the brains compared to infected wild-type controls   (MGI Ref ID J:194065)
  • increased granulocyte number
    • mice show a decrease in the numbers of dendritic cells and monocytes/macrophages and an increased accumulation of granulocytic myeloid cells after Mesocestoides corti infection compared with wild-type mice   (MGI Ref ID J:194065)
    • granulocytes from Mesocestoides corti infected mutants exhibit a regulatory phenotype   (MGI Ref ID J:194065)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Mrc1tm1Mnz/Mrc1tm1Mnz

        involves: 129P2/OlaHsd
  • immune system phenotype
  • abnormal macrophage physiology
    • migration distance and speed of bone marrow macrophages is increased compared to that of wild-type cells   (MGI Ref ID J:124290)
    • migration speed of bone marrow macrophages in response to colony stimulating factor-1 (CSF-1) is increased relative to that of wild-type cells   (MGI Ref ID J:124290)
    • however, membrane ruffling stimulated by CSF-1 is normal   (MGI Ref ID J:124290)
  • hematopoietic system phenotype
  • abnormal macrophage physiology
    • migration distance and speed of bone marrow macrophages is increased compared to that of wild-type cells   (MGI Ref ID J:124290)
    • migration speed of bone marrow macrophages in response to colony stimulating factor-1 (CSF-1) is increased relative to that of wild-type cells   (MGI Ref ID J:124290)
    • however, membrane ruffling stimulated by CSF-1 is normal   (MGI Ref ID J:124290)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Metabolism Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Mrc1tm1Mnz
Allele Name targeted mutation 1, Michel C Nussenzweig
Allele Type Targeted (Null/Knockout, Reporter)
Common Name(s) CD206-; MR-;
Mutation Made By Kang Liu,   The Rockefeller University
Strain of Origin129P2/OlaHsd
ES Cell Line NameE14
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name Mrc1, mannose receptor, C type 1
Chromosome 2
Gene Common Name(s) AW259686; CD206; CLEC13D; CLEC13DL; MMR; MR; MRC1L1; bA541I19.1; expressed sequence AW259686;
Molecular Note Exon 1 was disrupted by an enhanced green fluorescent protein (eGFP) gene inserted at the endogenous start codon. A single loxP site remained downstream of eGFP after the removal of a floxed neo cassette. Western blot analysis of lymph node lysates indicated an absence of normal protein in homozygous mutant mice. [MGI Ref ID J:75364]

Genotyping

Genotyping Information

Genotyping Protocols

Mrc1tm1Mnzalternate1,

MELT



Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Lee SJ; Evers S; Roeder D; Parlow AF; Risteli J; Risteli L; Lee YC; Feizi T; Langen H; Nussenzweig MC. 2002. Mannose receptor-mediated regulation of serum glycoprotein homeostasis. Science 295(5561):1898-901. [PubMed: 11884756]  [MGI Ref ID J:75364]

Additional References

Mrc1tm1Mnz related

Akilov OE; Kasuboski RE; Carter CR; McDowell MA. 2007. The role of mannose receptor during experimental leishmaniasis. J Leukoc Biol 81(5):1188-96. [PubMed: 17261547]  [MGI Ref ID J:121722]

Burgdorf S; Kautz A; Bohnert V; Knolle PA; Kurts C. 2007. Distinct pathways of antigen uptake and intracellular routing in CD4 and CD8 T cell activation. Science 316(5824):612-6. [PubMed: 17463291]  [MGI Ref ID J:121094]

Burgdorf S; Lukacs-Kornek V; Kurts C. 2006. The mannose receptor mediates uptake of soluble but not of cell-associated antigen for cross-presentation. J Immunol 176(11):6770-6. [PubMed: 16709836]  [MGI Ref ID J:131796]

Burgdorf S; Scholz C; Kautz A; Tampe R; Kurts C. 2008. Spatial and mechanistic separation of cross-presentation and endogenous antigen presentation. Nat Immunol 9(5):558-66. [PubMed: 18376402]  [MGI Ref ID J:134501]

Chavele KM; Martinez-Pomares L; Domin J; Pemberton S; Haslam SM; Dell A; Cook HT; Pusey CD; Gordon S; Salama AD. 2010. Mannose receptor interacts with Fc receptors and is critical for the development of crescentic glomerulonephritis in mice. J Clin Invest 120(5):1469-78. [PubMed: 20407205]  [MGI Ref ID J:161470]

Court N; Vasseur V; Vacher R; Fremond C; Shebzukhov Y; Yeremeev VV; Maillet I; Nedospasov SA; Gordon S; Fallon PG; Suzuki H; Ryffel B; Quesniaux VF. 2010. Partial redundancy of the pattern recognition receptors, scavenger receptors, and C-type lectins for the long-term control of Mycobacterium tuberculosis infection. J Immunol 184(12):7057-70. [PubMed: 20488784]  [MGI Ref ID J:161134]

Dan JM; Kelly RM; Lee CK; Levitz SM. 2008. Role of the mannose receptor in a murine model of Cryptococcus neoformans infection. Infect Immun 76(6):2362-7. [PubMed: 18391001]  [MGI Ref ID J:136129]

Doz E; Rose S; Nigou J; Gilleron M; Puzo G; Erard F; Ryffel B; Quesniaux VF. 2007. Acylation Determines the Toll-like receptor (TLR)-dependent Positive Versus TLR2-, Mannose Receptor-, and SIGNR1-independent Negative Regulation of Pro-inflammatory Cytokines by Mycobacterial Lipomannan. J Biol Chem 282(36):26014-26025. [PubMed: 17617634]  [MGI Ref ID J:124650]

Emara M; Royer PJ; Abbas Z; Sewell HF; Mohamed GG; Singh S; Peel S; Fox J; Shakib F; Martinez-Pomares L; Ghaemmaghami AM. 2011. Recognition of the Major Cat Allergen Fel d 1 through the Cysteine-rich Domain of the Mannose Receptor Determines Its Allergenicity. J Biol Chem 286(15):13033-40. [PubMed: 21335554]  [MGI Ref ID J:171135]

Everts B; Hussaarts L; Driessen NN; Meevissen MH; Schramm G; van der Ham AJ; van der Hoeven B; Scholzen T; Burgdorf S; Mohrs M; Pearce EJ; Hokke CH; Haas H; Smits HH; Yazdanbakhsh M. 2012. Schistosome-derived omega-1 drives Th2 polarization by suppressing protein synthesis following internalization by the mannose receptor. J Exp Med 209(10):1753-67, S1. [PubMed: 22966004]  [MGI Ref ID J:191421]

Geier H; Celli J. 2011. Phagocytic Receptors Dictate Phagosomal Escape and Intracellular Proliferation of Francisella tularensis. Infect Immun 79(6):2204-14. [PubMed: 21422184]  [MGI Ref ID J:171926]

Jansen KM; Pavlath GK. 2006. Mannose receptor regulates myoblast motility and muscle growth. J Cell Biol 174(3):403-13. [PubMed: 16864654]  [MGI Ref ID J:111306]

Lee SJ; Zheng NY; Clavijo M; Nussenzweig MC. 2003. Normal Host Defense during Systemic Candidiasis in Mannose Receptor-Deficient Mice. Infect Immun 71(1):437-45. [PubMed: 12496194]  [MGI Ref ID J:80986]

Lukacs-Kornek V; Burgdorf S; Diehl L; Specht S; Kornek M; Kurts C. 2008. The kidney-renal lymph node-system contributes to cross-tolerance against innocuous circulating antigen. J Immunol 180(2):706-15. [PubMed: 18178808]  [MGI Ref ID J:130952]

Madsen DH; Leonard D; Masedunskas A; Moyer A; Jurgensen HJ; Peters DE; Amornphimoltham P; Selvaraj A; Yamada SS; Brenner DA; Burgdorf S; Engelholm LH; Behrendt N; Holmbeck K; Weigert R; Bugge TH. 2013. M2-like macrophages are responsible for collagen degradation through a mannose receptor-mediated pathway. J Cell Biol 202(6):951-66. [PubMed: 24019537]  [MGI Ref ID J:201738]

Marttila-Ichihara F; Turja R; Miiluniemi M; Karikoski M; Maksimow M; Niemela J; Martinez-Pomares L; Salmi M; Jalkanen S. 2008. Macrophage mannose receptor on lymphatics controls cell trafficking. Blood 112(1):64-72. [PubMed: 18434610]  [MGI Ref ID J:137315]

Mishra PK; Morris EG; Garcia JA; Cardona AE; Teale JM. 2013. Increased accumulation of regulatory granulocytic myeloid cells in mannose receptor C type 1-deficient mice correlates with protection in a mouse model of neurocysticercosis. Infect Immun 81(4):1052-63. [PubMed: 23319563]  [MGI Ref ID J:194065]

Moseman AP; Moseman EA; Schworer S; Smirnova I; Volkova T; von Andrian U; Poltorak A. 2013. Mannose receptor 1 mediates cellular uptake and endosomal delivery of CpG-motif containing oligodeoxynucleotides. J Immunol 191(11):5615-24. [PubMed: 24184555]  [MGI Ref ID J:207009]

Ng WC; Liong S; Tate MD; Irimura T; Denda-Nagai K; Brooks AG; Londrigan SL; Reading PC. 2014. The macrophage galactose-type lectin can function as an attachment and entry receptor for influenza virus. J Virol 88(3):1659-72. [PubMed: 24257596]  [MGI Ref ID J:205282]

Ritter M; Gross O; Kays S; Ruland J; Nimmerjahn F; Saijo S; Tschopp J; Layland LE; Prazeres da Costa C. 2010. Schistosoma mansoni triggers Dectin-2, which activates the Nlrp3 inflammasome and alters adaptive immune responses. Proc Natl Acad Sci U S A 107(47):20459-64. [PubMed: 21059925]  [MGI Ref ID J:166590]

Segura E; Albiston AL; Wicks IP; Chai SY; Villadangos JA. 2009. Different cross-presentation pathways in steady-state and inflammatory dendritic cells. Proc Natl Acad Sci U S A 106(48):20377-81. [PubMed: 19918052]  [MGI Ref ID J:155577]

Shi S; Blumenthal A; Hickey CM; Gandotra S; Levy D; Ehrt S. 2005. Expression of many immunologically important genes in Mycobacterium tuberculosis-infected macrophages is independent of both TLR2 and TLR4 but dependent on IFN-alphabeta receptor and STAT1. J Immunol 175(5):3318-28. [PubMed: 16116224]  [MGI Ref ID J:113220]

Sly WS; Vogler C; Grubb JH; Levy B; Galvin N; Tan Y; Nishioka T; Tomatsu S. 2006. Enzyme therapy in mannose receptor-null mucopolysaccharidosis VII mice defines roles for the mannose 6-phosphate and mannose receptors. Proc Natl Acad Sci U S A 103(41):15172-7. [PubMed: 17015822]  [MGI Ref ID J:115263]

Sturge J; Todd SK; Kogianni G; McCarthy A; Isacke CM. 2007. Mannose receptor regulation of macrophage cell migration. J Leukoc Biol 82(3):585-93. [PubMed: 17596337]  [MGI Ref ID J:124290]

Swain SD; Lee SJ; Nussenzweig MC; Harmsen AG. 2003. Absence of the macrophage mannose receptor in mice does not increase susceptibility to Pneumocystis carinii infection in vivo. Infect Immun 71(11):6213-21. [PubMed: 14573639]  [MGI Ref ID J:86274]

Viriyakosol S; Jimenez Mdel P; Saijo S; Fierer J. 2014. Neither dectin-2 nor the mannose receptor is required for resistance to Coccidioides immitis in mice. Infect Immun 82(3):1147-56. [PubMed: 24379281]  [MGI Ref ID J:209412]

Yano J; Palmer GE; Eberle KE; Peters BM; Vogl T; McKenzie AN; Fidel PL Jr. 2014. Vaginal epithelial cell-derived S100 alarmins induced by Candida albicans via pattern recognition receptor interactions are sufficient but not necessary for the acute neutrophil response during experimental vaginal candidiasis. Infect Immun 82(2):783-92. [PubMed: 24478092]  [MGI Ref ID J:209808]

Zietara N; Lyszkiewicz M; Puchalka J; Pei G; Gutierrez MG; Lienenklaus S; Hobeika E; Reth M; Martins dos Santos VA; Krueger A; Weiss S. 2013. Immunoglobulins drive terminal maturation of splenic dendritic cells. Proc Natl Acad Sci U S A 110(6):2282-7. [PubMed: 23345431]  [MGI Ref ID J:194334]

deSchoolmeester ML; Martinez-Pomares L; Gordon S; Else KJ. 2009. The mannose receptor binds Trichuris muris excretory/secretory proteins but is not essential for protective immunity. Immunology 126(2):246-55. [PubMed: 18624733]  [MGI Ref ID J:145485]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

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