Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N?pN1 Generation Definitions   Donating Investigator David L. Garbers,   U.T. Southwestern Medical Center

Description
Homozygotes are viable. Males, but not females, are completely infertile. The targeted mutation fails to significantly alter sperm production, protein tyrosine phosphorylation associated with capacitation, induction of the acrosome reaction, forward velocity or percentage of motility. Sperm cells fail to acquire hyperactivated motility associated with penetration of the extracellular matrix of the egg. SDS/PAGE and immunoblotting of total sperm protein were used to confirm the absence of gene product. This strain may be useful in studies of male fertility.

Development
A targeting vector was designed to replace exons 2-5 of the gene with a neomycin drug resistance cassette. The vector was electroporated into 129S6/SvEvTac-derived SM1 embryonic stem (ES) cells. Chimeric mice were generated and backcrossed once to C57BL/6 to produce heterozygotes.

Additional Web Information

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI

- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Deafness, Sensorineural, and Male Infertility   (CATSPER2) Spermatogenic Failure 7; SPGF7   (CATSPER2)

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Catsper2^tm1Gar/Catsper2^tm1Gar

        involves: 129S6/SvEvTac * C57BL/6J

• reproductive system phenotype
• asthenozoospermia
  • although mutant sperm exhibit normal forward velocity and percentage of motile cells, they are unable to generate the hyperactivated form of sperm motility required for fertilization, as revealed by the absence of high track velocity and nonlinear trajectory spermatozoa in mutant sperm cell populations   (MGI Ref ID J:86972)
  • in mutant sperm, normal forward velocity is maintained despite a slight reduction in average flagellar amplitude and a small increase in the rate of flagellar beat   (MGI Ref ID J:86972)
  • in a high-viscosity medium, mutant sperm lose their ability to swim forward, whereas wild-type sperm remain progressively motile with reductions in forward velocity of ~50%   (MGI Ref ID J:86972)
• impaired acrosome reaction
  • mutant sperm are unable to penetrate the zona pellucida, despite normal forward velocity and percentage of motile cells   (MGI Ref ID J:86972)
  • however, mutant sperm exhibit normal protein tyrosine phosphorylation associated with capacitation, and initiate a normal acrosome reaction in response to intact zona pellucida   (MGI Ref ID J:86972)
• impaired fertilization
  • mutant sperm are unable to fertilize eggs with an intact zona pellucida both in vivo and in vitro   (MGI Ref ID J:86972)
  • in contrast, mutant sperm are able to fertilize eggs in which the zona pellucida has been removed   (MGI Ref ID J:86972)
• male infertility
  • male, but not female, homozygotes are incompletely infertile under normal breeding and in vitro conditions   (MGI Ref ID J:86972)
  • however, mutant males display no significant differences in body and testis weights, testis and sperm morphology or epididymal sperm counts relative to wild-type littermates   (MGI Ref ID J:86972)

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Catsper2^tm1Gar/Catsper2^tm1Gar

        involves: 129S6/SvEvTac

• reproductive system phenotype
• abnormal sperm physiology
  • calcium-selective pH sensitive sperm current (I_CatSper) is not detectable   (MGI Ref ID J:116406)
• • abnormal sperm motility
    • percent motility of mutant sperm decreases more rapidly than wild-type   (MGI Ref ID J:116406)
  • • asthenozoospermia
      • after 90 minutes in viscous conditions, sperm lose their motility compared to wild-type which move more vigorously than initially   (MGI Ref ID J:116406)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Reproductive Biology Research
Fertility Defects
      males only

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Catsper2tm1Gar
Allele Name		targeted mutation 1, David L Garbers
Allele Type		Targeted (knock-out)
Common Name(s)		CatSper2-;
Mutation Made By		David Garbers,   U.T. Southwestern Medical Center
Strain of Origin		129S6/SvEvTac
ES Cell Line Name		SM1
ES Cell Line Strain		129S6/SvEvTac
Gene Symbol and Name		Catsper2, cation channel, sperm associated 2
Chromosome		2
Molecular Note		A neomycin selection cassette replaced a genomic fragment encompassing exons 2 through 5. The deleted region included the start codon and encoded the first three transmembrane helices. Protein was undetected in homozygous mutant mice by Western blot and immunocytochemical analyses of spermatozoa. [MGI Ref ID J:86972]

Genotyping

Genotyping Information

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Quill TA; Sugden SA; Rossi KL; Doolittle LK; Hammer RE; Garbers DL. 2003. Hyperactivated sperm motility driven by CatSper2 is required for fertilization. Proc Natl Acad Sci U S A 100(25):14869-74. [PubMed: 14657366]  [MGI Ref ID J:86972]

Additional References

Catsper2^tm1Gar related

Carlson AE; Quill TA; Westenbroek RE; Schuh SM; Hille B; Babcock DF. 2005. Identical phenotypes of CatSper1 and CatSper2 null sperm. J Biol Chem 280(37):32238-44. [PubMed: 16036917]  [MGI Ref ID J:128548]

Ho K; Wolff CA; Suarez SS. 2009. CatSper-null mutant spermatozoa are unable to ascend beyond the oviductal reservoir. Reprod Fertil Dev 21(2):345-50. [PubMed: 19210926]  [MGI Ref ID J:151121]

Marquez B; Ignotz G; Suarez SS. 2007. Contributions of extracellular and intracellular Ca2+ to regulation of sperm motility: Release of intracellular stores can hyperactivate CatSper1 and CatSper2 null sperm. Dev Biol 303(1):214-21. [PubMed: 17174296]  [MGI Ref ID J:118812]

Qi H; Moran MM; Navarro B; Chong JA; Krapivinsky G; Krapivinsky L; Kirichok Y; Ramsey IS; Quill TA; Clapman DE. 2007. All four CatSper ion channel proteins are required for male fertility and sperm cell hyperactivated motility Proc Natl Acad Sci U S A :. [PubMed: 17227845]  [MGI Ref ID J:116406]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering Information
JAX^® Mice
Surgical and Preconditioning Services
JAX^® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Use restrictions apply, see Policy on Licensing and Use Restrictions.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.