Strain Name:

129S-Atp8b3tm1Gar/J

Stock Number:

007659

Availability:

Repository-Cryopreserved
-Sperm morphology and motility are unaffected, but tests show that phosphatidylserine (PS) already exists in the outer membrane leaflet before sperm capacitation. When the zona pellucida (ZP) is removed from eggs, fertilization rates are normal; although when the extracellular matrix is intact, fewer spermatozoa bind tightly or penetrate the ZP, and fewer undergo acrosome reactions. This strain may be useful in studies of male fertility.

Description

Strain Information

Former Names 129S6/SvEvTac-Atp8b3tm1Gar/J    (Changed: 25-SEP-07 )
Type Coisogenic; Mutant Strain; Targeted Mutation;
Specieslaboratory mouse
 
Donating Investigator David Garbers,   U.T. Southwestern Medical Center

Description
Both male and female homozygotes are fertile, but the average litter sizes from homozygous males are slightly smaller than observed with wildtype males. Sperm morphology and motility are unaffected, but tests show that phosphatidylserine (PS) already exists in the outer membrane leaflet before sperm capacitation. When the zona pellucida (ZP) is removed from eggs, fertilization rates are normal; although when the extracellular matrix is intact, fewer spermatozoa bind tightly or penetrate the ZP, and fewer undergo acrosome reactions. Homozygous mice do not express protein as determined by Western blot analysis of testis tissues and immunostaining of mature spermatozoa. This published information is based on studies of mixed background C57BL/6 and 129S6 background animals; the line offered here is on a pure 129S6 background. This strain may be useful in studies of male fertility.

Development
A targeting vector was designed to replace exons 4-14, encoding the first four transmembrane segments, with a neomycin resistance cassette. The vector was electroporated into 129S6/SvEvTac-derived SM1 embryonic stem (ES) cells. Chimeric mice were crossed with 129S6/SvEvTac animals.

Phenotype

Phenotype Information

Mammalian Phenotype Terms assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Atp8b3tm1Gar/Atp8b3tm1Gar

        involves: 129S/SvEv * C57BL/6J
  • reproductive system phenotype
  • abnormal fertilization (MGI Ref ID J:88796)
    • impaired sperm-egg interaction
    • normal rates of fertilization rates were observed when spermatozoa were added to zona pellucida-free eggs
    • premature exposure of phosphatidylserine on the outer membrane leaflet of spermatozoa
    • normal sperm morphology and motility
    • impaired acrosome reaction (MGI Ref ID J:88796)
      • reduced zona pellucida induced acrosome reactions
  • decreased litter size (MGI Ref ID J:88796)
    • fewer pups born to mutant male mice
  • reduced male fertility (MGI Ref ID J:88796)

Research Applications

This mouse can be used to support research in many areas including:

Reproductive Biology Research
Fertility Defects (males only)

Genes & Alleles

Gene & Allele Information

Allele Symbol Atp8b3tm1Gar
Allele Name targeted mutation 1, David L Garbers
Common Name(s) SAPLT-;
Mutation Made By David Garbers,   U.T. Southwestern Medical Center
Strain of Origin129S/SvEv
ES Cell Line Strain129
Gene Symbol and Name Atp8b3, ATPase, class I, type 8B, member 3
Chromosome 10
Gene Common Name(s) 1700042F02Rik; 1700056N23Rik; ATPIK; MGC188630; RIKEN cDNA 1700042F02 gene; RIKEN cDNA 1700056N23 gene; SAPLT;
Molecular Note A genomic fragment encompassing exons 4 through 14 was replaced with a neomycin selection cassette. The deleted region encoded the first four transmembrane domains of the protein. No protein was detected in homozygous mutant mice by Western blot analysisof testes membrane extracts or by immunostaining of spermatozoa. [MGI Ref ID J:88796]

Genotyping

Genotyping Information

Genotyping Protocols

Atp8b3tm1Gar, SEP PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Wang L; Beserra C; Garbers DL. 2004. A novel aminophospholipid transporter exclusively expressed in spermatozoa is required for membrane lipid asymmetry and normal fertilization. Dev Biol 267(1):203-15. [PubMed: 14975727]  [MGI Ref ID J:88796]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations             View   International   Pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $1900.00
*Price(s) in US dollars ($)

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • This strain is included in the Induced Mutant Resource Colony collection.

Pricing for International shipping destinations             View   USA, Canada and Mexico   Pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $2470.00
*Price(s) in US dollars ($)

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • This strain is included in the Induced Mutant Resource Colony collection.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering and Purchasing Information

      Purchasing Information
      JAX® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form


(3.1.1)