Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6;129S6-Slc9a10tm1Gar/J    (Changed: 06-DEC-12 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N?pN1 Generation Definitions   Donating Investigator David L. Garbers,   U.T. Southwestern Medical Center

Description
Homozygous females are fully fertile, but males are completely infertile and have severely diminished sperm motility. Body weight, testis size, and caudal epididymal sperm counts of null male mice are no different from those of wildtype or heterozygous littermates. No apparent defects in spermatogenesis were found. The addition of ammonium chloride, which elevates intracellular pH, partially rescues the motility and fertility defects. Cyclic AMP analogues almost completely rescue the motility and infertility phenotypes. Northern blot analysis confirmed the absence of full-length mRNA in homozygous mutants, but a truncated product was found. No stable protein was detected in null mice by immunohistochemistry or western blot analysis. This strain may be useful in studies of male fertility and the molecular basis of fertilization.

Development
A targeting vector was designed to replace part of exon 4 and exons 5-11 with a neomycin resistance cassette. The targeting vector was electroporated into 129S6/SvEvTac-derived SM1 embryonic stem (ES) cells. Targeted ES cells were injected into C57BL/6 blastocysts. Male chimeras were crossed to C57BL/6J females. This line was maintained on a mixed C57BL/6 and 129S6 background by the donating investigator.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Slc9c1^tm1Gar/Slc9c1^tm1Gar

        involves: 129S6/SvEvTac * C57BL/6J

• reproductive system phenotype
• asthenozoospermia
  • sperm are morphologically normal but display little or no forward movement   (MGI Ref ID J:87248)
  • incubation with ammonium chloride (to increase pH) or cAMP analogs can improve motility   (MGI Ref ID J:87248)
• male infertility
  • males but not females are infertile   (MGI Ref ID J:87248)
  • incubation of sperm with ammonium chloride (to increase pH) or cAMP analogs can partially restore fertility in vitro   (MGI Ref ID J:87248)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Reproductive Biology Research
Fertility Defects
      males only

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Slc9c1tm1Gar
Allele Name		targeted mutation 1, David L Garbers
Allele Type		Targeted (knock-out)
Common Name(s)		NHE-;
Mutation Made By		David Garbers,   U.T. Southwestern Medical Center
Strain of Origin		129S6/SvEvTac
ES Cell Line Name		SM1
ES Cell Line Strain		129S6/SvEvTac
Gene Symbol and Name		Slc9c1, solute carrier family 9, subfamily C (Na+-transporting carboxylic acid decarboxylase), member 1
Chromosome		16
Gene Common Name(s)		Gm610; LOC208169; NHE; SLC9A10; Slc9a10; gene model 610, (NCBI); solute carrier family 9, member 10; sperm-NHE; spermNHE;
Molecular Note		Part of exon 4 and exons 5-11 were replaced with a neo cassette. Northern blot confirmed the presence of a truncated transcript in mutant mice. Neither immunohistochemistry nor Western blot analysis showed the presence of a protein product of this allele. [MGI Ref ID J:87248]

Genotyping

Genotyping Information

Genotyping Protocols

Slc9a10^tm1Gar, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Kushner JA; Haj FG; Klaman LD; Dow MA; Kahn BB; Neel BG; White MF. 2004. Islet-Sparing Effects of Protein Tyrosine Phosphatase-1b Deficiency Delays Onset of Diabetes in IRS2 Knockout Mice. Diabetes 53(1):61-66. [PubMed: 14693698]  [MGI Ref ID J:87249]

Additional References

Slc9c1^tm1Gar related

Wang D; Hu J; Bobulescu IA; Quill TA; McLeroy P; Moe OW; Garbers DL. 2007. A sperm-specific Na+/H+ exchanger (sNHE) is critical for expression and in vivo bicarbonate regulation of the soluble adenylyl cyclase (sAC). Proc Natl Acad Sci U S A 104(22):9325-30. [PubMed: 17517652]  [MGI Ref ID J:122299]

Wang D; King SM; Quill TA; Doolittle LK; Garbers DL. 2003. A new sperm-specific Na+/H+ exchanger required for sperm motility and fertility. Nat Cell Biol 5(12):1117-22. [PubMed: 14634667]  [MGI Ref ID J:87248]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering Information
JAX^® Mice
Surgical and Preconditioning Services
JAX^® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.