Strain Name:

B6;129S6-Slc9c1tm1Gar/J

Stock Number:

007661

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Availability:

Cryopreserved - Ready for recovery

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Homozygous females are fully fertile, but males are completely infertile and have severely diminished sperm motility. The addition of ammonium chloride, which elevates intracellular pH, partially rescues the motility and fertility defects. Cyclic AMP analogues almost completely rescue the motility and infertility phenotypes. This strain may be useful in studies of male fertility and the molecular basis of fertilization.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6;129S6-Slc9a10tm1Gar/J    (Changed: 06-DEC-12 )
Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
GenerationN?pN1
Generation Definitions
 
Donating Investigator David L. Garbers,   U.T. Southwestern Medical Center

Description
Homozygous females are fully fertile, but males are completely infertile and have severely diminished sperm motility. Body weight, testis size, and caudal epididymal sperm counts of null male mice are no different from those of wildtype or heterozygous littermates. No apparent defects in spermatogenesis were found. The addition of ammonium chloride, which elevates intracellular pH, partially rescues the motility and fertility defects. Cyclic AMP analogues almost completely rescue the motility and infertility phenotypes. Northern blot analysis confirmed the absence of full-length mRNA in homozygous mutants, but a truncated product was found. No stable protein was detected in null mice by immunohistochemistry or western blot analysis. This strain may be useful in studies of male fertility and the molecular basis of fertilization.

Development
A targeting vector was designed to replace part of exon 4 and exons 5-11 with a neomycin resistance cassette. The targeting vector was electroporated into 129S6/SvEvTac-derived SM1 embryonic stem (ES) cells. Targeted ES cells were injected into C57BL/6 blastocysts. Male chimeras were crossed to C57BL/6J females. This line was maintained on a mixed C57BL/6 and 129S6 background by the donating investigator.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Slc9c1tm1Gar/Slc9c1tm1Gar

        involves: 129S6/SvEvTac * C57BL/6J
  • reproductive system phenotype
  • asthenozoospermia
    • sperm are morphologically normal but display little or no forward movement   (MGI Ref ID J:87248)
    • incubation with ammonium chloride (to increase pH) or cAMP analogs can improve motility   (MGI Ref ID J:87248)
  • male infertility
    • males but not females are infertile   (MGI Ref ID J:87248)
    • incubation of sperm with ammonium chloride (to increase pH) or cAMP analogs can partially restore fertility in vitro   (MGI Ref ID J:87248)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Reproductive Biology Research
Fertility Defects
      males only

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Slc9c1tm1Gar
Allele Name targeted mutation 1, David L Garbers
Allele Type Targeted (knock-out)
Common Name(s) NHE-;
Mutation Made By David Garbers,   U.T. Southwestern Medical Center
Strain of Origin129S6/SvEvTac
ES Cell Line NameSM1
ES Cell Line Strain129S6/SvEvTac
Gene Symbol and Name Slc9c1, solute carrier family 9, subfamily C (Na+-transporting carboxylic acid decarboxylase), member 1
Chromosome 16
Gene Common Name(s) Gm610; LOC208169; NHE; SLC9A10; Slc9a10; gene model 610, (NCBI); solute carrier family 9, member 10; sperm-NHE; spermNHE;
Molecular Note Part of exon 4 and exons 5-11 were replaced with a neo cassette. Northern blot confirmed the presence of a truncated transcript in mutant mice. Neither immunohistochemistry nor Western blot analysis showed the presence of a protein product of this allele. [MGI Ref ID J:87248]

Genotyping

Genotyping Information

Genotyping Protocols

Slc9a10tm1Gar, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Kushner JA; Haj FG; Klaman LD; Dow MA; Kahn BB; Neel BG; White MF. 2004. Islet-Sparing Effects of Protein Tyrosine Phosphatase-1b Deficiency Delays Onset of Diabetes in IRS2 Knockout Mice. Diabetes 53(1):61-66. [PubMed: 14693698]  [MGI Ref ID J:87249]

Additional References

Slc9c1tm1Gar related

Wang D; Hu J; Bobulescu IA; Quill TA; McLeroy P; Moe OW; Garbers DL. 2007. A sperm-specific Na+/H+ exchanger (sNHE) is critical for expression and in vivo bicarbonate regulation of the soluble adenylyl cyclase (sAC). Proc Natl Acad Sci U S A 104(22):9325-30. [PubMed: 17517652]  [MGI Ref ID J:122299]

Wang D; King SM; Quill TA; Doolittle LK; Garbers DL. 2003. A new sperm-specific Na+/H+ exchanger required for sperm motility and fertility. Nat Cell Biol 5(12):1117-22. [PubMed: 14634667]  [MGI Ref ID J:87248]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2085.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2710.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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