Description

Strain Information

Type Mutant Stock; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Hemizygote         (Female x Male) Species laboratory mouse Generation ?+F1 (08-JAN-08)   Donating Investigator Philippe Soriano,   Fred Hutchinson Cancer Research Center

Description
These mice possess loxP sites flanking exons 2 through 5 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 through 5 deleted in the cre-expressing tissue(s). These Efnb1 conditional mutant mice may be useful in studying cellular signaling in embryonic development and adult mice; specifically receptor tyrosine kinases.

For example, when crossed to a strain expressing Cre recombinase in epiblast-derived tissues (see Stock No. 003755), this mutant mouse strain may be useful in embryogenesis research.

Development
A targeting vector containing sequence encoding exons 2 through 5 of the endogenous gene and a FRT flanked PGKneo selection cassette was utilized in the construction of this mutant. Similarly oriented loxP sites were placed upstream of exon 2 and downstream of exon 5. The FRT flanked selection cassette was inserted downstream of exon 5. The construct was electroporated into 129S4/SvJaeSor-derived AK7.1 embryonic stem (ES) cells Correctly targeted ES cells were injected into recipient blastocysts. The mice were crossed with 129S4/SvJaeSor mice for 3 generations.

Control Information

	Control
	002448 129S1/SvImJ	(approximate)
Considerations for Choosing Controls

Additional Web Information

Cre-lox Systems

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX^® Services jaxservices@jax.org for customized breeding options.

Efnb1^tm1Sor/Efnb1⁺ Meox2^tm1(cre)Sor/Meox2⁺

        involves: 129S4/SvJaeSor * C57BL/6   (conditional)

• lethality-postnatal
• postnatal lethality (MGI Ref ID J:89008)
  • less than Mendelian ratios of mice are recovered at 2 weeks after birth
• limbs/digits/tail phenotype
• polydactyly (MGI Ref ID J:89008)
  • 100% of mice have polydactyly restricted to digits I or II of either the hindlimbs or forelimbs
• skeleton phenotype
• abnormal perichondrium morphology (MGI Ref ID J:89008)
  • the perichondrium around forming digits is poorly organized

Efnb1^tm1Sor/Efnb1⁺ Meox2^tm1(cre)Sor/Meox2⁺

        involves: 129S4/SvJaeSor   (conditional)

• lethality-postnatal
• postnatal lethality (MGI Ref ID J:89008)
  • less than Mendelian ratios of mice are recovered at 2 weeks after birth
• limbs/digits/tail phenotype
• polydactyly (MGI Ref ID J:89008)
  • 100% of mice have polydactyly restricted to digits I or II of either the hindlimbs or forelimbs
  • on the 129S4/SvJaeSor background defects are restricted to only the last phalange

Efnb1^tm1Sor/Efnb1^tm1Sor Meox2^tm1(cre)Sor/Meox2⁺

        involves: 129S4/SvJaeSor   (conditional)

• lethality-postnatal
• postnatal lethality (MGI Ref ID J:89008)
  • less than Mendelian ratios of mice are recovered at 2 weeks after birth

Efnb1^tm1Sor/Y Meox2^tm1(cre)Sor/Meox2⁺

        involves: 129S4/SvJaeSor * C57BL/6   (conditional)

• lethality-postnatal
• postnatal lethality (MGI Ref ID J:89008)
  • less than Mendelian ratios of mice are recovered at 2 weeks after birth
  • postnatal lethality is more severe in a 129S4/SvJaeSor background than in a mixed 129S4/SvJaeSor C57BL/6 background

Efnb1^tm1Sor/Y Meox2^tm1(cre)Sor/Meox2⁺

        involves: 129S4/SvJaeSor   (conditional)

• lethality-postnatal
• postnatal lethality (MGI Ref ID J:89008)
  • less than Mendelian ratios of mice are recovered at 2 weeks after birth
  • postnatal lethality is more severe in a 129S4/SvJaeSor background than in a mixed 129S4/SvJaeSor C57BL/6 background

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cell Biology Research
Signal Transduction

Research Tools
Cre-lox System (loxP-flanked Sequences)
Developmental Biology Research (Cre-lox System)

Genes & Alleles

Gene & Allele Information

Allele Symbol		Efnb1tm1Sor
Allele Name		targeted mutation 1, Philippe Soriano
Allele Type		Targeted (Floxed/Frt)
Common Name(s)		ephrin-B1lox;
Mutation Made By		Philippe Soriano,   Fred Hutchinson Cancer Research Center
Strain of Origin		129S4/SvJaeSor
ES Cell Line Name		AK7
ES Cell Line Strain		129S4/SvJaeSor
Gene Symbol and Name		Efnb1, ephrin B1
Chromosome		X
Gene Common Name(s)		CFND; CFNS; Cek5 ligand; Cek5-L; EFL-3; EFL3; EPLG2; Elk-L; Epl2; LERK-2; LERK2; MGC8782; Stra1; eph-related receptor tyrosine kinase ligand 2;
Molecular Note		Exons 2 through 5 were flanked by single loxP sites inserted by homologous recombination. A PGK-neo-bpA cassette flanked by FRT sites remained downstream of the coding region. [MGI Ref ID J:89008]

Genotyping

Genotyping Information

Genotyping Protocols

Efnb1^tm1Sor, STD PCR, vers. 1
NEOTD (Generic Neo), STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Davy A; Aubin J; Soriano P. 2004. Ephrin-B1 forward and reverse signaling are required during mouse development. Genes Dev 18(5):572-83. [PubMed: 15037550]  [MGI Ref ID J:89008]

Additional References

Efnb1^tm1Sor related

Cortina C; Palomo-Ponce S; Iglesias M; Fernandez-Masip JL; Vivancos A; Whissell G; Huma M; Peiro N; Gallego L; Jonkheer S; Davy A; Lloreta J; Sancho E; Batlle E. 2007. EphB-ephrin-B interactions suppress colorectal cancer progression by compartmentalizing tumor cells. Nat Genet 39(11):1376-83. [PubMed: 17906625]  [MGI Ref ID J:130035]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as homozygotes.
Mating System	Homozygote x Hemizygote         (Female x Male)
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	002448 129S1/SvImJ	(approximate)
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

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