Strain Name: |
B6.129S4(Cg)-Arntltm1Weit/J |
|---|---|
Stock Number: |
007668 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
B6.129S4-Arntltm1Weit/J (Changed: 12-SEP-07
) |
| Genes & Alleles | Arntl; Arntltm1Weit; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Charles Weitz, Harvard Medical School Strain Description
Mice homozygous for this conditional (floxed) allele possess loxP sites flanking exon 8 of the targeted gene and are viable and fertile, with circadian behavioral rhythms indistinguishable from wildtype littermates. When bred to mice that express Cre recombinase, the resulting offspring will have the exon encoding the ARNTL (BMAL1) basic helix-loop-helix (bHLH) domain deleted in the cre-expressing tissue(s). These Bmal1-floxed mutant mice may be useful in generating conditional mutations (whole-mouse or tissue-specific) to study the role of circadian clock/circadian rhythm in physiological and behavioral regulation.For example, when crossed to a strain expressing Cre recombinase in the retina (see Stock No. 005105), this mutant mouse strain may be useful in studies of the circadian clock of the retina.
Strain Development
Using homologous recombination in a bacterial artificial chromosome (BAC, clone RPCI23-331K23), an FRT-flanked neo cassette (followed by a loxP site) was inserted immediately downstream of exon 8 of the targeted gene. An additional loxP site was added upstream of exon 8 when the targeting vector was excised from the BAC. This construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and chimeric mice were bred to Flpe-expressing mice (see Stock No. 003946) to excise the neo cassette. The resulting offspring were next bred to C57BL/6 congenic mutant mice (non-germline cre mice). After this, mice were selectively backcrossed to C57BL/6J inbred mice for at least 6 generations to contain only the conditional mutation (loxP-flanked exon 8 with a single remaining FRT site) prior to arrival at The Jackson Laboratory.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Arntltm1Weit | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Charles Weitz | ||
| Common Name(s) | conditional Bmal; | ||
| Mutation Made By | Charles Weitz, Harvard Medical School | ||
| Strain of Origin | 129S4/SvJae | ||
| ES Cell Line Name | J1 | ||
| ES Cell Line Strain | 129S4/SvJae | ||
| Gene Symbol and Name | Arntl, aryl hydrocarbon receptor nuclear translocator-like | ||
| Chromosome | 7 | ||
| Gene Common Name(s) | Arnt3; BMAL1; BMAL1c; JAP3; MGC47515; MOP3; PASD3; TIC; bHLHe5; | ||
| Molecular Note | Using homologous recombination in a bacterial artificial chromosome (BAC, clone RPCI23-331K23), an FRT-flanked neo cassette (followed by a loxP site) was inserted immediately downstream of exon 8 of the targeted gene. An additional loxP site was added upstream of exon 8 when the targeting vector was excised from the BAC. This construct was electroporated into J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and chimeric mice were bred to Flpe-expressing mice to excise the neo cassette. [MGI Ref ID J:124157] | ||
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Arntltm1Weit
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, homozygous mice may be bred. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Arntl
007618 B6.Cg-Tg(tetO-Arntl)1Jt/J View Strains carrying other alleles of Arntl (1 strain)
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
Neurobiology Research
Circadian Rhythms
Cre-lox System (loxP-flanked Sequences)
Research Tools
Cardiovascular Research (Cre-lox System)
Cre-lox System (loxP-flanked Sequences)
Developmental Biology Research (Cre-lox System)
Diabetes and Obesity Research (loxP)
Reproductive Biology Research (Cre-lox System)
References
Selected Reference(s)
Storch KF; Paz C; Signorovitch J; Raviola E; Pawlyk B; Li T; Weitz CJ. 2007. Intrinsic circadian clock of the Mammalian retina: importance for retinal processing of visual information. Cell 130(4):730-41. [PubMed: 17719549] [MGI Ref ID J:124157]
Price and Supply Information
| Strain Name: | B6.129S4(Cg)-Arntltm1Weit/J |
| Stock Number: | 007668 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
- Use of MICE by companies or for-profit entities requires a license.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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