Strain Name: |
B6.129S4-Pdgfratm11(EGFP)Sor/J |
|---|---|
Stock Number: |
007669 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Pdgfra; Pdgfratm11(EGFP)Sor; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Heterozygote x +/+ sibling (Female x Male) Species laboratory mouse Donating Investigator Philippe Soriano, Fred Hutchinson Cancer Research Center Generation N17+N1F1 (25-APR-08) Strain Description
Mice homozygous for this knock-in targeted mutation have an embryonic lethal phenotype, with half of the embryos failing to survive past embryonic day 12.5 and the remainder failing to survive beyond embryonic day 15.5. These mice express the H2B-eGFP fusion gene from the endogenous Pdgfra locus. Fluorescence is detectable at embryonic day 4.5 in polar trophectoderm cells and at embryonic day 6.5 in the extraembryonic ectoderm. Expression of H2BGFP mimick the expression pattern of the endogenous gene. Homozygotes exhibit abnormal placenta development and placenta vasculature. This mutant mouse strain may be useful in studies of cellular signaling during development and in adult mice; specifically receptor tyrosine kinases (RTK; such as Ras, MAP kinase, PI3K and those in the platelet-derived growth factor (PDGF) family).Strain Development
A targeting vector containing a human H2B histone protein and enhanced Green Fluorescent Protein fusion gene, H2B-GFP or H2B-eGFP, and a floxed PGKneo selection cassette was used to disrupt sequence of the targeted gene that encodes the signal peptide and the first two immunoglobulin domains of the targeted gene. The endogenous Pdgfra promoter drives expression of the H2B-eGFP fusion gene. The construct was electroporated into 129S4/SvJaeSor derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were backcrossed to C57BL/6 mice for 17 generations.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Pdgfratm11(EGFP)Sor | ||
|---|---|---|---|
| Allele Name | targeted mutation 11, Philippe Soriano | ||
| Common Name(s) | PdgfalpharGFP; alphaGFP; | ||
| Strain of Origin | 129S4/SvJaeSor | ||
| ES Cell Line Name | AK7 | ||
| ES Cell Line Strain | 129S4/SvJaeSor | ||
| Gene Symbol and Name | Pdgfra, platelet derived growth factor receptor, alpha polypeptide | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | AI115593; APDGFR; CD140A; MGC74795; PDGFACE; PDGFR2; Pdgfr-2; Rhe-PDGFRA; expressed sequence AI115593; | ||
| Molecular Note | The gene was disrupted by a knock-in vector consisting of an enhanced GFP gene and a PGK-neo cassette. [J:83560] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice can be bred as heterozygotes. Homozygotes have an embryonic lethal phenotype. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
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006492 B6.Cg-Pdgfratm8Sor/EiJ
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
Cell Biology Research
Signal Transduction
Developmental Biology Research
Embryonic Lethality (Homozygous)
Research Tools
Cell Biology Research
Developmental Biology Research
Fluorescent Proteins
References
Selected Reference(s)
Additional ReferencesHamilton TG; Klinghoffer RA; Corrin PD; Soriano P. 2003. Evolutionary divergence of platelet-derived growth factor alpha receptor signaling mechanisms. Mol Cell Biol 23(11):4013-25. [PubMed: 12748302] [J:83560]
Price and Supply Information
| Strain Name: | B6.129S4-Pdgfratm11(EGFP)Sor/J |
| Stock Number: | 007669 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. No age specifications accepted. Colony sized to produce minimal quantities (typically up to 6 mice) upon order receipt; one order per strain. Expected delivery: 1-3 months. Larger quantities or custom orders arranged upon request. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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