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| These mice express the H2B-eGFP fusion gene from the endogenous Pdgfra locus. Fluorescence patterns mimick the expression pattern of the endogenous gene. Homozygotes exhibit abnormal placenta development and placenta vasculature. This mutant mouse strain may be useful in studies of cellular signaling during development and in adult mice; specifically receptor tyrosine kinases (RTK; such as Ras, MAP kinase, PI3K and those in the platelet-derived growth factor (PDGF) family). | |||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
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Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Heterozygote x +/+ sibling (Female x Male) 30-DEC-07 Species laboratory mouse Generation N17+N1F2 (31-DEC-08) Donating Investigator Philippe Soriano, Mount Sinai School of Medicine Description
Mice homozygous for this knock-in targeted mutation have an embryonic lethal phenotype, with half of the embryos failing to survive past embryonic day 12.5 and the remainder failing to survive beyond embryonic day 15.5. These mice express the H2B-eGFP fusion gene from the endogenous Pdgfra locus. Fluorescence is detectable at embryonic day 4.5 in polar trophectoderm cells and at embryonic day 6.5 in the extraembryonic ectoderm. Expression of H2BGFP mimick the expression pattern of the endogenous gene. Homozygotes exhibit abnormal placenta development and placenta vasculature. This mutant mouse strain may be useful in studies of cellular signaling during development and in adult mice; specifically receptor tyrosine kinases (RTK; such as Ras, MAP kinase, PI3K and those in the platelet-derived growth factor (PDGF) family).Development
A targeting vector containing a human H2B histone protein and enhanced Green Fluorescent Protein fusion gene, H2B-GFP or H2B-eGFP, and a floxed PGKneo selection cassette was used to disrupt sequence of the targeted gene that encodes the signal peptide and the first two immunoglobulin domains of the targeted gene. The endogenous Pdgfra promoter drives expression of the H2B-eGFP fusion gene. The construct was electroporated into 129S4/SvJaeSor derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were backcrossed to C57BL/6 mice for 17 generations.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
006492 B6.Cg-Pdgfratm8Sor/EiJ
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Pdgfratm11(EGFP)Sor/Pdgfratm11(EGFP)Sor
involves: 129S4/SvJaeSor * C57BL/6
- lethality-prenatal/perinatal
- prenatal lethality (MGI Ref ID J:83560)
- embryogenesis phenotype
- abnormal placenta development (MGI Ref ID J:83560)
- the epithelial cell layer between the chorioallantoic plate and labyrinth is completely absent
- abnormal placenta vasculature (MGI Ref ID J:83560)
- vascular development in the chorioallantoic plate is retarded
This mouse can be used to support research in many areas including:
Cell Biology Research
Signal Transduction
Developmental Biology Research
Embryonic Lethality (Homozygous)
Research Tools
Cell Biology Research
Developmental Biology Research
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Pdgfratm11(EGFP)Sor | ||
|---|---|---|---|
| Allele Name | targeted mutation 11, Philippe Soriano | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | PdgfalphaGFP; PdgfalpharGFP; alphaGFP; | ||
| Strain of Origin | 129S4/SvJaeSor | ||
| ES Cell Line Name | AK7 | ||
| ES Cell Line Strain | 129S4/SvJaeSor | ||
| Site of Expression | Expression of the H2B-EGFP fusion protein mimicks the expression pattern of the endogenous Pdgfra gene. Fluorescence is detectable at embryonic day 4.5 in polar trophectoderm cells and at embryonic day 6.5 in the extraembryonic ectoderm. | ||
| Gene Symbol and Name | Pdgfra, platelet derived growth factor receptor, alpha polypeptide | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | AI115593; APDGFR; CD140A; MGC74795; PDGFACE; PDGFR2; Pdgfr-2; RHEPDGFRA; expressed sequence AI115593; | ||
| Molecular Note | The gene was disrupted by a knock-in vector consisting of an enhanced GFP gene and a PGK-neo cassette. [MGI Ref ID J:83560] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Pdgfratm11(EGFP)Sor, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Hamilton TG; Klinghoffer RA; Corrin PD; Soriano P. 2003. Evolutionary divergence of platelet-derived growth factor alpha receptor signaling mechanisms. Mol Cell Biol 23(11):4013-25. [PubMed: 12748302] [MGI Ref ID J:83560]
Additional References
Pdgfratm11(EGFP)Sor relatedFrench WJ; Creemers EE; Tallquist MD. 2008. Platelet-derived growth factor receptors direct vascular development independent of vascular smooth muscle cell function. Mol Cell Biol 28(18):5646-57. [PubMed: 18606782] [MGI Ref ID J:140245]
Klinghoffer RA; Hamilton TG; Hoch R; Soriano P. 2002. An allelic series at the PDGFalphaR locus indicates unequal contributions of distinct signaling pathways during development. Dev Cell 2(1):103-13. [PubMed: 11782318] [MGI Ref ID J:73817]
Mandeville I; Aubin J; LeBlanc M; Lalancette-Hebert M; Janelle MF; Tremblay GM; Jeannotte L. 2006. Impact of the loss of Hoxa5 function on lung alveogenesis. Am J Pathol 169(4):1312-27. [PubMed: 17003488] [MGI Ref ID J:113378]
Perl AK; Gale E. 2009. FGF signaling is required for myofibroblast differentiation during alveolar regeneration. Am J Physiol Lung Cell Mol Physiol 297(2):L299-308. [PubMed: 19502291] [MGI Ref ID J:151422]
Pickett EA; Olsen GS; Tallquist MD. 2008. Disruption of PDGFR{alpha}-initiated PI3K activation and migration of somite derivatives leads to spina bifida. Development 135(3):589-98. [PubMed: 18192285] [MGI Ref ID J:131172]
Richarte AM; Mead HB; Tallquist MD. 2007. Cooperation between the PDGF receptors in cardiac neural crest cell migration. Dev Biol 306(2):785-96. [PubMed: 17499702] [MGI Ref ID J:122584]
Tallquist MD; French WJ; Soriano P. 2003. Additive Effects of PDGF Receptor beta Signaling Pathways in Vascular Smooth Muscle Cell Development. PLoS Biol 1(2):288-99. [PubMed: 14624252] [MGI Ref ID J:86542]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as heterozygotes. Homozygotes have an embryonic lethal phenotype. Mating System Heterozygote x +/+ sibling (Female x Male) 30-DEC-07 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Heterozygous for Pdgfratm11(EGFP)Sor
Pairs /Price (US dollars $) Pair Genotype $297.85 Heterozygous for Pdgfratm11(EGFP)Sor x Wild-type for Pdgfratm11(EGFP)Sor $297.85 Wild-type for Pdgfratm11(EGFP)Sor x Heterozygous for Pdgfratm11(EGFP)Sor
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Heterozygous for Pdgfratm11(EGFP)Sor
Pairs /Price (US dollars $) Pair Genotype $387.30 Heterozygous for Pdgfratm11(EGFP)Sor x Wild-type for Pdgfratm11(EGFP)Sor $387.30 Wild-type for Pdgfratm11(EGFP)Sor x Heterozygous for Pdgfratm11(EGFP)Sor
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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