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| These mice express the H2B-eGFP fusion gene from the endogenous Pdgfra locus. Fluorescence patterns mimick the expression pattern of the endogenous gene. Homozygotes exhibit abnormal placenta development and placenta vasculature. This mutant mouse strain may be useful in studies of cellular signaling during development and in adult mice; specifically receptor tyrosine kinases (RTK; such as Ras, MAP kinase, PI3K and those in the platelet-derived growth factor (PDGF) family). | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Heterozygote x +/+ sibling (Female x Male) 30-DEC-07 Species laboratory mouse Generation N17+N1F9 (24-MAR-11)
Generation DefinitionsDonating Investigator Dr. Philippe Soriano, Mount Sinai School of Medicine Description
Mice homozygous for this knock-in targeted mutation have an embryonic lethal phenotype, with half of the embryos failing to survive past embryonic day 12.5 and the remainder failing to survive beyond embryonic day 15.5. These mice express the H2B-eGFP fusion gene from the endogenous Pdgfra locus. Fluorescence is detectable at embryonic day 4.5 in polar trophectoderm cells and at embryonic day 6.5 in the extraembryonic ectoderm. Expression of H2BGFP mimick the expression pattern of the endogenous gene. Homozygotes exhibit abnormal placenta development and placenta vasculature. This mutant mouse strain may be useful in studies of cellular signaling during development and in adult mice; specifically receptor tyrosine kinases (RTK; such as Ras, MAP kinase, PI3K and those in the platelet-derived growth factor (PDGF) family).Development
A targeting vector containing a human H2B histone protein and enhanced Green Fluorescent Protein fusion gene, H2B-GFP or H2B-eGFP, and a floxed PGKneo selection cassette was used to disrupt sequence of the targeted gene that encodes the signal peptide and the first two immunoglobulin domains of the targeted gene. The endogenous Pdgfra promoter drives expression of the H2B-eGFP fusion gene. The construct was electroporated into 129S4/SvJaeSor derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were backcrossed to C57BL/6 mice for 17 generations.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (357 strains)
018433 B6.129S4-Pdgfratm12Sor/J 006492 B6.Cg-Pdgfratm8Sor/EiJ 018280 B6N.Cg-Tg(Pdgfra-cre/ERT)467Dbe/J 013148 C57BL/6-Tg(Pdgfra-cre)1Clc/J 018432 STOCK Pdgfratm13Sor/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Gastrointestinal Stromal Tumor; GIST (PDGFRA)
Hypereosinophilic Syndrome, Idiopathic; HES (PDGFRA)
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Pdgfratm11(EGFP)Sor/Pdgfratm11(EGFP)Sor
involves: 129S4/SvJaeSor * C57BL/6
- mortality/aging
- prenatal lethality (MGI Ref ID J:83560)
- embryogenesis phenotype
- abnormal placenta development
- the epithelial cell layer between the chorioallantoic plate and labyrinth is completely absent (MGI Ref ID J:83560)
- abnormal placenta vasculature
- vascular development in the chorioallantoic plate is retarded (MGI Ref ID J:83560)
- cardiovascular system phenotype
- abnormal placenta vasculature
- vascular development in the chorioallantoic plate is retarded (MGI Ref ID J:83560)
This mouse can be used to support research in many areas including:
GFP relatedCell Biology Research
Signal Transduction
Developmental Biology Research
Embryonic Lethality (Homozygous)
Research Tools
Cell Biology Research
Developmental Biology Research
Fluorescent Proteins
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Pdgfratm11(EGFP)Sor | ||
|---|---|---|---|
| Allele Name | targeted mutation 11, Philippe Soriano | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | PDGFRalphaEGFP; PdgfalphaGFP; PdgfalpharGFP; PdgfraH2B-GFP; alphaGFP; | ||
| Strain of Origin | 129S4/SvJaeSor | ||
| ES Cell Line Name | AK7 | ||
| ES Cell Line Strain | 129S4/SvJaeSor | ||
| Site of Expression | Expression of the H2B-EGFP fusion protein mimicks the expression pattern of the endogenous Pdgfra gene. Fluorescence is detectable at embryonic day 4.5 in polar trophectoderm cells and at embryonic day 6.5 in the extraembryonic ectoderm. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Molecular Note | The gene was disrupted by a knock-in vector consisting of an enhanced GFP gene and a PGK-neo cassette. [MGI Ref ID J:83560] | ||
| Gene Symbol and Name | Pdgfra, platelet derived growth factor receptor, alpha polypeptide | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | AI115593; APDGFR; CD140A; PDGFACE; PDGFR-2; PDGFR2; Pdgfr-2; RHEPDGFRA; expressed sequence AI115593; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Pdgfratm11(EGFP)Sor, Melt Curve Analysis
Pdgfratm11(EGFP)Sor, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Hamilton TG; Klinghoffer RA; Corrin PD; Soriano P. 2003. Evolutionary divergence of platelet-derived growth factor alpha receptor signaling mechanisms. Mol Cell Biol 23(11):4013-25. [PubMed: 12748302] [MGI Ref ID J:83560]
Additional References
Pdgfratm11(EGFP)Sor relatedAcharya A; Baek ST; Huang G; Eskiocak B; Goetsch S; Sung CY; Banfi S; Sauer MF; Olsen GS; Duffield JS; Olson EN; Tallquist MD. 2012. The bHLH transcription factor Tcf21 is required for lineage-specific EMT of cardiac fibroblast progenitors. Development 139(12):2139-49. [PubMed: 22573622] [MGI Ref ID J:185552]
Artus J; Piliszek A; Hadjantonakis AK. 2011. The primitive endoderm lineage of the mouse blastocyst: sequential transcription factor activation and regulation of differentiation by Sox17. Dev Biol 350(2):393-404. [PubMed: 21146513] [MGI Ref ID J:170416]
Bax NA; Bleyl SB; Gallini R; Wisse LJ; Hunter J; Van Oorschot AA; Mahtab EA; Lie-Venema H; Goumans MJ; Betsholtz C; Gittenberger-de Groot AC. 2010. Cardiac malformations in Pdgfralpha mutant embryos are associated with increased expression of WT1 and Nkx2.5 in the second heart field. Dev Dyn 239(8):2307-17. [PubMed: 20658695] [MGI Ref ID J:162870]
Berry R; Rodeheffer MS. 2013. Characterization of the adipocyte cellular lineage in vivo. Nat Cell Biol 15(3):302-8. [PubMed: 23434825] [MGI Ref ID J:195198]
Chojnacki A; Mak G; Weiss S. 2011. PDGFR{alpha} Expression Distinguishes GFAP-Expressing Neural Stem Cells from PDGF-Responsive Neural Precursors in the Adult Periventricular Area. J Neurosci 31(26):9503-12. [PubMed: 21715615] [MGI Ref ID J:174061]
Clarke LE; Young KM; Hamilton NB; Li H; Richardson WD; Attwell D. 2012. Properties and fate of oligodendrocyte progenitor cells in the corpus callosum, motor cortex, and piriform cortex of the mouse. J Neurosci 32(24):8173-85. [PubMed: 22699898] [MGI Ref ID J:185577]
Fredriksson L; Nilsson I; Su EJ; Andrae J; Ding H; Betsholtz C; Eriksson U; Lawrence DA. 2012. Platelet-derived growth factor C deficiency in C57BL/6 mice leads to abnormal cerebral vascularization, loss of neuroependymal integrity, and ventricular abnormalities. Am J Pathol 180(3):1136-44. [PubMed: 22230248] [MGI Ref ID J:181950]
French WJ; Creemers EE; Tallquist MD. 2008. Platelet-derived growth factor receptors direct vascular development independent of vascular smooth muscle cell function. Mol Cell Biol 28(18):5646-57. [PubMed: 18606782] [MGI Ref ID J:140245]
Grabarek JB; Zyzynska K; Saiz N; Piliszek A; Frankenberg S; Nichols J; Hadjantonakis AK; Plusa B. 2012. Differential plasticity of epiblast and primitive endoderm precursors within the ICM of the early mouse embryo. Development 139(1):129-39. [PubMed: 22096072] [MGI Ref ID J:179000]
Greif DM; Kumar M; Lighthouse JK; Hum J; An A; Ding L; Red-Horse K; Espinoza FH; Olson L; Offermanns S; Krasnow MA. 2012. Radial construction of an arterial wall. Dev Cell 23(3):482-93. [PubMed: 22975322] [MGI Ref ID J:189012]
Kang M; Piliszek A; Artus J; Hadjantonakis AK. 2013. FGF4 is required for lineage restriction and salt-and-pepper distribution of primitive endoderm factors but not their initial expression in the mouse. Development 140(2):267-79. [PubMed: 23193166] [MGI Ref ID J:191065]
Klinghoffer RA; Hamilton TG; Hoch R; Soriano P. 2002. An allelic series at the PDGFalphaR locus indicates unequal contributions of distinct signaling pathways during development. Dev Cell 2(1):103-13. [PubMed: 11782318] [MGI Ref ID J:73817]
Lee YH; Petkova AP; Mottillo EP; Granneman JG. 2012. In vivo identification of bipotential adipocyte progenitors recruited by beta3-adrenoceptor activation and high-fat feeding. Cell Metab 15(4):480-91. [PubMed: 22482730] [MGI Ref ID J:184459]
Mandeville I; Aubin J; LeBlanc M; Lalancette-Hebert M; Janelle MF; Tremblay GM; Jeannotte L. 2006. Impact of the loss of Hoxa5 function on lung alveogenesis. Am J Pathol 169(4):1312-27. [PubMed: 17003488] [MGI Ref ID J:113378]
Mellgren AM; Smith CL; Olsen GS; Eskiocak B; Zhou B; Kazi MN; Ruiz FR; Pu WT; Tallquist MD. 2008. Platelet-derived growth factor receptor beta signaling is required for efficient epicardial cell migration and development of two distinct coronary vascular smooth muscle cell populations. Circ Res 103(12):1393-401. [PubMed: 18948621] [MGI Ref ID J:155272]
Perl AK; Gale E. 2009. FGF signaling is required for myofibroblast differentiation during alveolar regeneration. Am J Physiol Lung Cell Mol Physiol 297(2):L299-308. [PubMed: 19502291] [MGI Ref ID J:151422]
Pickett EA; Olsen GS; Tallquist MD. 2008. Disruption of PDGFR{alpha}-initiated PI3K activation and migration of somite derivatives leads to spina bifida. Development 135(3):589-98. [PubMed: 18192285] [MGI Ref ID J:131172]
Richarte AM; Mead HB; Tallquist MD. 2007. Cooperation between the PDGF receptors in cardiac neural crest cell migration. Dev Biol 306(2):785-96. [PubMed: 17499702] [MGI Ref ID J:122584]
Rock JR; Barkauskas CE; Cronce MJ; Xue Y; Harris JR; Liang J; Noble PW; Hogan BL. 2011. Multiple stromal populations contribute to pulmonary fibrosis without evidence for epithelial to mesenchymal transition. Proc Natl Acad Sci U S A 108(52):E1475-83. [PubMed: 22123957] [MGI Ref ID J:180047]
Tallquist MD; French WJ; Soriano P. 2003. Additive Effects of PDGF Receptor beta Signaling Pathways in Vascular Smooth Muscle Cell Development. PLoS Biol 1(2):288-99. [PubMed: 14624252] [MGI Ref ID J:86542]
Walton KD; Kolterud A; Czerwinski MJ; Bell MJ; Prakash A; Kushwaha J; Grosse AS; Schnell S; Gumucio DL. 2012. Hedgehog-responsive mesenchymal clusters direct patterning and emergence of intestinal villi. Proc Natl Acad Sci U S A 109(39):15817-22. [PubMed: 23019366] [MGI Ref ID J:190319]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as heterozygotes. Homozygotes have an embryonic lethal phenotype. Mating System Heterozygote x +/+ sibling (Female x Male) 30-DEC-07 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Heterozygous for Pdgfratm11(EGFP)Sor
Price per Pair (US dollars $) Pair Genotype $296.00 Heterozygous for Pdgfratm11(EGFP)Sor x Wild-type for Pdgfratm11(EGFP)Sor $296.00 Wild-type for Pdgfratm11(EGFP)Sor x Heterozygous for Pdgfratm11(EGFP)Sor Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Heterozygous for Pdgfratm11(EGFP)Sor
Price per Pair (US dollars $) Pair Genotype $384.80 Heterozygous for Pdgfratm11(EGFP)Sor x Wild-type for Pdgfratm11(EGFP)Sor $384.80 Wild-type for Pdgfratm11(EGFP)Sor x Heterozygous for Pdgfratm11(EGFP)Sor Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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|
|
Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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