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| These mutant mice express a site-specific recombinase, PhiC31o, that has been targeted to the endogenous Gt(ROSA)26Sor locus and codon-optimized for expression in mouse tissues. PhiC31o mediates irreversible DNA recombination at specific nonidentical pairs of sequence sites, attB and attP. When crossed with a strain containing a sequence of interest flanked by attB and attP sites, PhiC31o-mediated recombination results in tissue-specific deletion of the targeted sequence. Recombination efficiency is similar to that of Cre recombinase. This strain represents an effective tool for generating tissue specific-targeted mutants. | |||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6;129S4-Gt(ROSA)26Sortm3(phiC31o)Sor/J (Changed: 16-JAN-08 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Heterozygote x Heterozygote (Female x Male) Species laboratory mouse Generation ?+F0 (07-JAN-08) Donating Investigator Philippe Soriano, Fred Hutchinson Cancer Research Center Description
These mutant mice express a site-specific recombinase, PhiC31o, that has been targeted to the endogenous Gt(ROSA)26Sor locus and codon-optimized for expression in mouse tissues. PhiC31o mediates irreversible DNA recombination at specific nonidentical pairs of sequence sites, attB and attP. When crossed with a strain containing a sequence of interest flanked by attB and attP sites, PhiC31o-mediated recombination results in tissue-specific deletion of the targeted sequence. Recombination efficiency is similar to that of Cre recombinase. This strain represents an effective tool for generating tissue specific-targeted mutants.Development
A targeting construct with the SA-PhiC310pA-PGKneopA cassette, which contains the mouse codon-optimized site-specific recombinase PhiC31o sequence (with a C-terminal SV40 nuclear localization signal) driven by the phosphoglycerate kinase 1 (PGK) promoter, and bovine growth hormone polyadenylation (bpA) sequence was inserted into the Gt(ROSA)26Sor locus via electroporation of 129S4/SvJaeSor-derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were identified and used to generate mutant mice. Homozygous mice were maintained on a 129S4/SvJaeSor background prior to arrival at The Jackson Laboratory. The mice were then crossed to C57BL/6J (Stock No. 000664) for one generation.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Gt(ROSA)26Sortm3(phiC31*)Sor allele
007743 B6.129S4-Gt(ROSA)26Sortm3(phiC31*)Sor/J View Strains carrying Gt(ROSA)26Sortm3(phiC31*)Sor (1 strain)
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Gt(ROSA)26Sortm3(phiC31*)Sor/Gt(ROSA)26Sortm3(phiC31*)Sor
involves: 129S4/SvJaeSor
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:128069)
- homozygotes are viable, fertile, and show no gross physical or behavioral abnormalities
This mouse can be used to support research in many areas including:
phiC31 relatedResearch Tools
PhiC31 System (PhiC31 Recombinase Expression)
Research Tools
PhiC31 System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Gt(ROSA)26Sortm3(phiC31*)Sor | ||
|---|---|---|---|
| Allele Name | targeted mutation 3, Philippe Soriano | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | R26phiC31lo; | ||
| Strain of Origin | 129S4/SvJaeSor | ||
| ES Cell Line Name | AK7 | ||
| ES Cell Line Strain | 129S4/SvJaeSor | ||
| Expressed Gene | phiC31, PhiC31 recombinase, | ||
| The site-specific recombinase PhiC31 is an integrase enzyme derived from the bacteriaphage phiC31 of Streptomyces lividans that mediates efficient irreversible DNA recombination at specific nonidentical pairs of sequence sites, attB and attP. After the excision event, the attB and attP sites are converted to hybrid attL or attR sites. | |||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| Molecular Note | A targeting construct with the SA-PhiC310pA-PGKneopA cassette, which contains the mouse codon-optimized site-specific recombinase PhiC31o sequence (with a C-terminal SV40 nuclear localization signal) driven by the phosphoglycerate kinase 1 (PGK) promoter, and bovine growth hormone polyadenylation (bpA) sequence was inserted into the Gt(ROSA)26Sor locus. PhiC31o-mediated recombination in mice carrying containing a sequence of interest flanked by attB and attP sites results in tissue-specific deletion of the targeted sequence. [MGI Ref ID J:128069] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sor tm3(phiC31*)Sor, tm4(attB/attP)Sor, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Raymond CS; Soriano P. 2007. High-efficiency FLP and PhiC31 site-specific recombination in mammalian cells. PLoS ONE 2(1):e162. [PubMed: 17225864] [MGI Ref ID J:128069]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as homozygotes. Mating System Heterozygote x Heterozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $236.40 Female or Male Heterozygous for Gt(ROSA)26Sortm3(phiC31*)Sor $291.90 Female or Male Homozygous for Gt(ROSA)26Sortm3(phiC31*)Sor *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $472.80 Heterozygous for Gt(ROSA)26Sortm3(phiC31*)Sor x Heterozygous for Gt(ROSA)26Sortm3(phiC31*)Sor
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $307.40 Female or Male Heterozygous for Gt(ROSA)26Sortm3(phiC31*)Sor $379.50 Female or Male Homozygous for Gt(ROSA)26Sortm3(phiC31*)Sor *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $614.70 Heterozygous for Gt(ROSA)26Sortm3(phiC31*)Sor x Heterozygous for Gt(ROSA)26Sortm3(phiC31*)Sor
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
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