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| These mutant mice express a site-specific recombinase, PhiC31o, that has been targeted to the endogenous Gt(ROSA)26Sor locus and codon-optimized for expression in mouse tissues. PhiC31o mediates irreversible DNA recombination at specific nonidentical pairs of sequence sites, attB and attP. When crossed with a strain containing a sequence of interest flanked by attB and attP sites, PhiC31o-mediated recombination results in tissue-specific deletion of the targeted sequence. Recombination efficiency is similar to that of Cre recombinase. This strain represents an effective tool for generating tissue specific-targeted mutants. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B6;129S4-Gt(ROSA)26Sortm3(phiC31o)Sor/J (Changed: 16-JAN-08 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation ?+F1pN1 Donating Investigator Philippe Soriano, Mount Sinai School of Medicine Description
These mutant mice express a site-specific recombinase, PhiC31o, that has been targeted to the endogenous Gt(ROSA)26Sor locus and codon-optimized for expression in mouse tissues. PhiC31o mediates irreversible DNA recombination at specific nonidentical pairs of sequence sites, attB and attP. When crossed with a strain containing a sequence of interest flanked by attB and attP sites, PhiC31o-mediated recombination results in tissue-specific deletion of the targeted sequence. Recombination efficiency is similar to that of Cre recombinase. This strain represents an effective tool for generating tissue specific-targeted mutants.Development
A targeting construct with the SA-PhiC310pA-PGKneopA cassette, which contains the mouse codon-optimized site-specific recombinase PhiC31o sequence (with a C-terminal SV40 nuclear localization signal) driven by the phosphoglycerate kinase 1 (PGK) promoter, and bovine growth hormone polyadenylation (bpA) sequence was inserted into the Gt(ROSA)26Sor locus via electroporation of 129S4/SvJaeSor-derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were identified and used to generate mutant mice. Homozygous mice were maintained on a 129S4/SvJaeSor background prior to arrival at The Jackson Laboratory. The mice were then crossed to C57BL/6J (Stock No. 000664) for one generation.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Gt(ROSA)26Sortm3(phiC31*)Sor allele
007743 B6.129S4-Gt(ROSA)26Sortm3(phiC31*)Sor/J View Strains carrying Gt(ROSA)26Sortm3(phiC31*)Sor (1 strain)
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Gt(ROSA)26Sortm3(phiC31*)Sor/Gt(ROSA)26Sortm3(phiC31*)Sor
involves: 129S4/SvJaeSor
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:128069)
- homozygotes are viable, fertile, and show no gross physical or behavioral abnormalities
This mouse can be used to support research in many areas including:
phiC31 relatedResearch Tools
PhiC31 System
PhiC31 Recombinase Expression
Research Tools
PhiC31 System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Gt(ROSA)26Sortm3(phiC31*)Sor | ||
|---|---|---|---|
| Allele Name | targeted mutation 3, Philippe Soriano | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | R26phiC31lo; | ||
| Strain of Origin | 129S4/SvJaeSor | ||
| ES Cell Line Name | AK7.1 | ||
| ES Cell Line Strain | 129S4/SvJaeSor | ||
| Expressed Gene | phiC31, PhiC31 recombinase, | ||
| The site-specific recombinase PhiC31 is an integrase enzyme derived from the bacteriaphage phiC31 of Streptomyces lividans that mediates efficient irreversible DNA recombination at specific nonidentical pairs of sequence sites, attB and attP. After the excision event, the attB and attP sites are converted to hybrid attL or attR sites. | |||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| Driver Note | Gt(ROSA)26Sor | ||
| Molecular Note | A targeting construct with the SA-PhiC310pA-PGKneopA cassette, which contains the mouse codon-optimized site-specific recombinase PhiC31o sequence (with a C-terminal SV40 nuclear localization signal) driven by the phosphoglycerate kinase 1 (PGK) promoter, and bovine growth hormone polyadenylation (bpA) sequence was inserted into the Gt(ROSA)26Sor locus. PhiC31o-mediated recombination in mice carrying containing a sequence of interest flanked by attB and attP sites results in tissue-specific deletion of the targeted sequence. [MGI Ref ID J:128069] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm1sor STD, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Raymond CS; Soriano P. 2007. High-efficiency FLP and PhiC31 site-specific recombination in mammalian cells. PLoS ONE 2(1):e162. [PubMed: 17225864] [MGI Ref ID J:128069]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as homozygotes.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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