Go to JAX^® Mice Query Form

General Terms and Conditions	Additional Licensing and Use Restrictions

Former Name	B6;129S4-Fgfr1tm5.1Sor/J    (Changed: 03-SEP-08 )
Genes & Alleles	Fgfr1;   Fgfr1tm5Sor;

---

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Stock Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Donating Investigator Philippe Soriano,   Fred Hutchinson Cancer Research Center Generation N5+F4 (05-AUG-08)

Strain Description
These mice possess loxP sites on either side of exon 4 of the targeted gene. Exon 4 is the first exon found in all reported Fgfr1 splice variants. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 4 and the neomycin selection cassette deleted in the cre-expressing tissue(s). This mutant mouse strain may be useful in studies of fibroblast growth factor (Fgf) cellular signaling during embryonic development.

Strain Development
A targeting vector containing Fgfr1 sequence, an FRT site flanked neomycin resistance gene and a diphtheria toxin gene was utilized in the construction of this mutant. Similarly oriented loxP sites were placed on either side of exon 4 and the neomycin selection cassette. The construct was electroporated into 129S4/SvJaeSor-derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to a Flp-expressing strain on the 129S4 background to remove the neo cassette and then backrossed to C57BL/6J mice for at least one generation.

Mammalian Phenotype Terms assigned by genotype Fgfr1tm5Sor/Fgfr1tm5Sor         involves: 129S4/SvJaeSor * C57BL/6J no phenotypic analysis *normal* no phenotypic analysis (MGI Ref ID J:105920) no details provided

Gene & Allele Details

Allele Symbol		Fgfr1tm5Sor
Allele Name		targeted mutation 5, Philippe Soriano
Strain of Origin		129S4/SvJaeSor
ES Cell Line Name		AK7
ES Cell Line Strain		129S4/SvJaeSor
Gene Symbol and Name		Fgfr1, fibroblast growth factor receptor 1
Chromosome		8
Gene Common Name(s)		AW208770; BFGFR; CD331; CEK; FGFBR; FLG; FLJ99988; FLT2; FMS-like tyrosine kinase 2; Fgfr-1; Flt-2; HBGFR; KAL2; N-SAM; expressed sequence AW208770;
Molecular Note		Exon 4 was flanked by loxP sites via homologous recombination. Exon 4 is the first allele common to all splice variants. [MGI Ref ID J:105920]

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Genotyping Protocols

Fgfr1^tm5Sor

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as homozygotes.
Diet Information	LabDiet® 5K52/5K67

Additional Web Information

Cre-lox or FLP-FRT Systems

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

Cell Biology Research
Signal Transduction

Developmental Biology Research

Research Tools
Cre-lox System (loxP-flanked Sequences)

References

Selected Reference(s)

Hoch RV; Soriano P. 2006. Context-specific requirements for Fgfr1 signaling through Frs2 and Frs3 during mouse development. Development 133(4):663-73. [PubMed: 16421190]  [MGI Ref ID J:105920]

---

Price and Supply Information

Strain Name:	B6.129S4-Fgfr1tm5.1Sor/J
Stock Number:	007671

Price Details

IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.

• USA, Canada and Mexico
• International

*NO Shipping Destination selected!

 

Supply Details

Standard Supply	Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes	Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.
Licensing	See General Terms and Conditions below for Licensing and Use Restrictions
Control Information	View Control Information in Strain Details.

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Go to JAX^® Mice Query Form