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Genes & Alleles

Man1a2;   Man1a2tm1.1Ahe;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System +/+ sibling x Heterozygote         (Female x Male) Species laboratory mouse Donating Investigator Annette Herscovics,   McGill University Generation ?+N1F1 (08-JAN-08)

Strain Description
Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous mice have a perinatal lethal phenotype and die within hours of birth due to respiratory distress (cyanosis and alveolar hemorrhage). No gene product (mRNA) is detected by RT-PCR analysis of total RNA from homozygous embryonic tissues. At embryonic day 18.5 homozygous embryos exhibit reduced alveolar air space, thickened alveolar walls, small atelectactic areas and delayed lung development. This mutant mouse strain may be useful in studies of lung development and pulmonary physiology.

Strain Development
A targeting vector, pflox, containing sequence encoding exon 2 and loxP site flanked neomycin resistance and herpes simplex virus thymidine kinase genes was utilized in the construction of this mutant. This construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a cre expression plasmid for the purpose of removing exon 2 and the selectable marker cassette. ES cells that had successfully undergone Cre recombination and no longer retained exon 2 or the selection cassette were were injected into C57BL/6 recipient blastocysts. Resulting chimeric animals were backcrossed to wildtype C57BL/6J mice.

Mammalian Phenotype Terms assigned by genotype Man1a2tm1.1Ahe/Man1a2tm1.1Ahe         involves: 129S4/SvJae * C57BL/6 lethality-prenatal/perinatal neonatal lethality (MGI Ref ID J:119977) neonates die within several hours of birth respiratory system phenotype abnormal lung development (MGI Ref ID J:119977) lung development is delayed, with E18.5 and neonatal lungs at the canalicular stage of development instead of at the saccular stage, as in wild type abnormal lung morphology (MGI Ref ID J:119977) decrease in alveolar air space at E18.5 thick alveolar septum (MGI Ref ID J:119977) thickening of alveolar septae is seen at E18.5 atelectasis (MGI Ref ID J:119977) small isolated atelectactic regions are occasionally seen in the lungs, however all lungs inflate sufficiently to briefly sustain life (4-10 hours) lung hemorrhage (MGI Ref ID J:119977) neonates exhibit alveolar hemorrhaging however, pulmonary capillary and blood vessel networks are normal respiratory distress (MGI Ref ID J:119977) within several hours of birth, progressively display signs of respiratory distress cardiovascular system phenotype lung hemorrhage (MGI Ref ID J:119977) neonates exhibit alveolar hemorrhaging however, pulmonary capillary and blood vessel networks are normal homeostasis/metabolism phenotype cyanosis (MGI Ref ID J:119977) within several hours of birth, display signs of cyanosis Man1a2tm1.1Ahe/Man1a2tm1.1Ahe         either: 129S4/SvJae-Man1a2tm1.1Ahe or (involves: 129S4/SvJae * C57BL/6 * CD-1) lethality-prenatal/perinatal neonatal lethality (MGI Ref ID J:119977) neonates die within several hours of birth respiratory system phenotype respiratory distress (MGI Ref ID J:119977) within several hours of birth, display signs of respiratory distress

Gene & Allele Details

Allele Symbol		Man1a2tm1.1Ahe
Allele Name		targeted mutation 1.1, Annette Herscovics
Common Name(s)		Man1B-;
Mutation Made By		Annette Herscovics,   McGill University
Strain of Origin		129S4/SvJae
ES Cell Line Name		J1
ES Cell Line Strain		129S4/SvJae
Gene Symbol and Name		Man1a2, mannosidase, alpha, class 1A, member 2
Chromosome		3
Gene Common Name(s)		AI428775; AI528764; AI854422; MAN1B; Man1b; PCR2; expressed sequence AI428775; expressed sequence AI528764; expressed sequence AI854422; mannosidase 1, beta;
Molecular Note		The floxed exon 2 in Man1a2tm1Ahe was removed via transient transfection with Cre recombinase. Excision of the floxed exon causes a frameshift and introduces a stop codon immediately following exon 1. RT-PCR confirmed the loss of gene expression in various tissues. [MGI Ref ID J:119977]

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J
Considerations for Choosing Controls

Genotyping Protocols

Man1a2^tm1.1Ahe

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice are bred as heterozygotes. Homozygotes have a perinatal lethal phenotype and die within hours of birth.
Diet Information	LabDiet® 5K52/5K67

Animal Health Reports

Room Number           AX11

Research Applications

This mouse can be used to support research in many areas including:

Developmental Biology Research
Internal/Organ Defects (lung)
Perinatal Lethality (Homozygous)

References

Selected Reference(s)

Tremblay LO; Nagy Kovacs E; Daniels E; Wong NK; Sutton-Smith M; Morris HR; Dell A; Marcinkiewicz E; Seidah NG; McKerlie C; Herscovics A. 2007. Respiratory distress and neonatal lethality in mice lacking Golgi alpha1,2-mannosidase IB involved in N-glycan maturation. J Biol Chem 282(4):2558-66. [PubMed: 17121831]  [MGI Ref ID J:119977]

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Price and Supply Information

Strain Name:	B6;129S4-Man1a2tm1.1Ahe/J
Stock Number:	007672

Price Details

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Supply Details

Standard Supply	Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes	Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.
Licensing	See General Terms and Conditions below for Licensing and Use Restrictions
Control Information	View Control Information in Strain Details.

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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