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| These GAD67-GFP (line 3) mice may be useful for fluorescent labeling of neurons and studying the development, maturation, regulation, and functional integration of newborn neurons in adult hippocampus. | |||||||||||
- Description
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- Genes & alleles
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Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 23-JAN-09 Species laboratory mouse Generation N7+F1 (03-FEB-09) Donating Investigator Guoping Feng, Duke University Medical Center Description
Mice hemizygous for this GAD67-GFP transgene are viable and fertile. In the hippocampus, GAD67-GFP (line 3) mice selectively express enhanced green fluorescent protein (EGFP) in newborn dentate granule cells. At two weeks of age, EGFP is expressed in large numbers of dentate granule cells, and the number of EGFP-positive cells diminishes with age in a pattern consistent with the postnatal development of dentate granule cells/age-related reduction in adult neurogenesis in the dentate gyrus. The EGFP expression in newborn dentate granule cells begins within the first week of cell division and disappears as newborn neurons mature (about 4 weeks postmitotic), and EGFP positive dentate granule cells also express the newborn neuron markers PSA-NCAM and doublecortin. The bright labeling of newborn neurons with EGFP makes it possible to visualize the details of dendrites, which reach the outer edge of the molecular layer, and their axon terminals (mossy fiber boutons) in the CA3 region. In addition, EGFP is expressed in GABAergic neurons in many regions of the brain including the Purkinje cells of the cerebellum, periglomerular neurons of the olfactory bulb, and interneurons of the superior colliculus and brainstem. In some regions of the brain (thalamus, hypothalamus, cortex and striatum) sparse EGFP labeling is seen, with all EGFP positive cells also GAD67 positive in these regions. EGFP expression is not reported in hippocampal interneurons, including those in the hilus. Because the transgene contains loxP sites surrounding the GAD67-GFP sequences, crossing to a cre expressing strain will result in offspring bearing a deletion of the GAD67-GFP transgene. These GAD67-GFP (line 3) mice may be useful for fluorescent labeling of neurons and studying the development, maturation, regulation, and functional integration of newborn neurons in adult hippocampus.Of note, this GAD67-GFP strain is one of many fluorescent GABAergic neuron strains, each with unique labeling characteristics (see Stock No. 003718, Stock No. 006334, Stock No. 006340, Stock No. 007614, and Stock No. 007677).
Development
The bacterial artificial chromosome (BAC) clone RP23-118N13 containing the mouse GAD67 gene (and 120 kb of upstream and 46 kb downstream sequence) was modified by insertion of an Enhanced Green Fluorescent Protein (EGFP) cDNA and polyadenylation sequence at the translation initiation site of the GAD67 gene. This GAD67-GFP BAC, flanked by loxP sites as a result of insertion into the vector, was microinjected into fertilized (C57BL/6J x SJL)F1 oocytes. Transgenic offspring were bred to C57BL/6J mice. Founders with appropriate GFP expression levels and patterns (founder line 3) were established and bred to C57BL/6 for at least six generations prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
007677 CB6-Tg(Gad1-EGFP)G42Zjh/J 003718 FVB-Tg(GadGFP)45704Swn/J 006334 STOCK Tg(Gad1-EGFP)94Agmo/J 006340 STOCK Tg(Gad1-EGFP)98Agmo/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
Tg(Gad1-EGFP)3Gfng/0
B6.SJL-Tg(Gad1-EGFP)3Gfng
- no phenotypic analysis
- *normal* no phenotypic analysis (MGI Ref ID J:143860)
- mice are viable and fertile; no detailed analysis is provided
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
Alzheimer's Disease
Fluorescent protein expression in neural tissue
Parkinson's Disease
Research Tools
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers: neurons
Neurobiology Research
cell marker
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Gad1-EGFP)3Gfng | ||
|---|---|---|---|
| Allele Name | transene insertion 3, Guoping Feng | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | GAD67-GFP; GAD67-GFP (line 3); GAD67-GFP BAC; | ||
| Mutation Made By | Guoping Feng, Duke University Medical Center | ||
| Strain of Origin | (C57BL/6J x SJL)F1 | ||
| Site of Expression | In newborn dentate granule cells in the hippocampus during development and in the adult. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Gad1, glutamic acid decarboxylase 1, mouse, laboratory | ||
| Molecular Note | The bacterial artificial chromosome (BAC) clone RP23-118N13 containing the mouse GAD67 gene (and 120 kb of upstream and 46 kb downstream sequence) was modified by insertion of an Enhanced Green Fluorescent Protein (EGFP) cDNA and polyadenylation sequenceat the translation initiation site of the GAD67 gene. This GAD67-GFP BAC was microinjected into fertilized (C57BL/6J x SJL)F1 oocytes. Founder line 3 was established. Selective transgene expression is observed in newborn dentate granule cells of the adult hippocampus (subgranular zone of the dentate gyrus). EGFP is strongly expressed in the Purkinje cells of the cerebellum, periglomerular neurons of the olfactory bulb, and interneurons of the superior colliculus and brainstem with fluorescence observed in neurons (including axon terminals, dendrites and dendritic spines). Sparse EGFP labeling is reported in the thalamus, hypothalamus, cortex and striatum. [MGI Ref ID J:143860] | ||
| Gene Symbol and Name | Tg(Gad1-EGFP)3Gfng, transene insertion 3, Guoping Feng | ||
| Chromosome | UN | ||
| Gene Common Name(s) | GAD67-GFP; GAD67-GFP (line 3); GAD67-GFP BAC; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Melt Curve Analysis
Fluorescent Proteins (Generic GFP), Standard PCR
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Tg(Gad1-EGFP)3Gfng, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Additional References
Tg(Gad1-EGFP)3Gfng relatedFeng G. 2009. Generation of a Gad1 reporter transgene MGI Direct Data Submission :. [MGI Ref ID J:143860]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice are bred to wildtype siblings or to C57BL/6J inbred mice. Mating System Noncarrier x Hemizygote (Female x Male) 23-JAN-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(Gad1-EGFP)3Gfng
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(Gad1-EGFP)3Gfng x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(Gad1-EGFP)3Gfng
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(Gad1-EGFP)3Gfng
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(Gad1-EGFP)3Gfng x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(Gad1-EGFP)3Gfng
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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