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| These GAD67-GFP (line G42) mice may be useful for fluorescent labeling of the Pv-expressing subset of GABAergic neurons, allowing reliable and efficient characterization of perisomatic innervation in vivo. | |||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered Mutant Mice. Mating System Noncarrier x Hemizygote (Female x Male) Species laboratory mouse Generation N?+F1 (08-JAN-08) Donating Investigator Z. Josh Huang, Cold Spring Harbor Laboratory Description
Mice hemizygous for this GAD67-GFP transgene are viable and fertile. GAD67-GFP (line G42) mice selectively express enhanced green fluorescent protein (EGFP) in the calcium-binding protein parvalbumin (Pv)-expressing subclass of basket interneurons (soma, dendrites, and axons) and also in putative presynaptic boutons. While transgene expression (EGFP expression) is published as early as postnatal day 0 (P0) with high expression levels throughout postnatal development, the donating investigator additionally reports transgene expression as early as embryonic day 14 (E14). EGFP expression is not reported in other interneuron classes positive for somatostatin (SOM), cholecystokinin (CCK), calretinin (CR), and VIP. These GAD67-GFP (line G42) mice may be useful for fluorescent labeling of the Pv-expressing subset of GABAergic neurons, allowing reliable and efficient characterization of perisomatic innervation in vivo.Of note, this GAD67-GFP strain is one of many fluorescent GABAergic neuron strains, each with unique labeling characteristics (see Stock No. 003718, Stock No. 006334, Stock No. 006340, Stock No. 007614, and Stock No. 007673).
Development
The bacterial artificial chromosome (BAC) clone 56I19 containing the mouse GAD67 gene (and 60 kb of upstream and downstream sequence) was modified by insertion of an Enhanced Green Fluorescent Protein (EGFP) cDNA and phosphoglycerate kinase polyadenylation sequence in the first coding exon at the translation initiation site of the GAD67 gene. This GAD67-GFP BAC was microinjected into fertilized C57BL/6 mouse eggs. Transgenic offspring with appropriate GFP expression levels and patterns (founder line G42) were established on the C57BL/6 genetic background. After many generations of breeding to C57BL/6, an increase in hydrocephalus incidence was observed on the coisogenic background. To eliminate the undesired phenotype, this colony was subsequently maintained by breeding hemizygous mice to CB6F1/J (Stock No. 100007) for multiple generations prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
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| Noncarrier | ||
| 100007 CB6F1/J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (170 strains)
003718 FVB-Tg(GadGFP)45704Swn/J 006334 STOCK Tg(Gad1-EGFP)94Agmo/J 006340 STOCK Tg(Gad1-EGFP)98Agmo/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Gad1-EGFP)G42Zjh/0
involves: C57BL/6
- no phenotypic analysis
- *normal* no phenotypic analysis (MGI Ref ID J:94812)
- only expression of transgene is studied
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
Alzheimer's Disease
Fluorescent protein expression in neural tissue
Parkinson's Disease
Research Tools
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: neurons)
Neurobiology Research (cell marker)
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Gad1-EGFP)G42Zjh | ||
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| Allele Name | transgene insertion G42, Z Josh Huang | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | GAD67-EGFP; GAD67-GFP (line G42); | ||
| Strain of Origin | C57BL/6 | ||
| Site of Expression | calcium-binding protein parvalbumin (Pv)-expressing subclass of basket interneurons (soma, dendrites, and axons)and putative presynaptic boutons; detected as early as embryonic day 14 (E14) with high expression levels throughout postnatal development; not reported in other interneuron classes positive for somatostatin (SOM), cholecystokinin (CCK), calretinin (CR), and VIP | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Gad1, glutamic acid decarboxylase 1, mouse, laboratory | ||
| Molecular Note | The bacterial artificial chromosome (BAC) clone 56I19 containing the mouse GAD67 gene (and 60 kb of upstream and downstream sequence) was modified by insertion of an Enhanced Green Fluorescent Protein (EGFP) cDNA and phosphoglycerate kinase polyadenylation sequence in the first coding exon at the translation initiation site of the GAD67 gene. This GAD67-GFP BAC was microinjected into fertilized C57BL/6 mouse eggs. Mice (line G42) selectively express enhanced green fluorescent protein (EGFP) in the calcium-binding protein parvalbumin (Pv)-expressing subclass of basket interneurons (soma, dendrites, and axons) and also in putative presynaptic boutons. Transgene expression (EGFP expression) is detected as early as embryonic day 14 (E14) with high expressionlevels throughout postnatal development. [MGI Ref ID J:93895] [MGI Ref ID J:94812] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Gad1-EGFP)G42Zjh, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Chattopadhyaya B; Di Cristo G; Higashiyama H; Knott GW; Kuhlman SJ; Welker E; Huang ZJ. 2004. Experience and activity-dependent maturation of perisomatic GABAergic innervation in primary visual cortex during a postnatal critical period. J Neurosci 24(43):9598-611. [PubMed: 15509747] [MGI Ref ID J:94812]
Additional References
Tg(Gad1-EGFP)G42Zjh relatedAngo F; di Cristo G; Higashiyama H; Bennett V; Wu P; Huang ZJ. 2004. Ankyrin-based subcellular gradient of neurofascin, an immunoglobulin family protein, directs GABAergic innervation at purkinje axon initial segment. Cell 119(2):257-72. [PubMed: 15479642] [MGI Ref ID J:93895]
Brennaman LH; Maness PF. 2008. Developmental regulation of GABAergic interneuron branching and synaptic development in the prefrontal cortex by soluble neural cell adhesion molecule. Mol Cell Neurosci 37(4):781-93. [PubMed: 18289872] [MGI Ref ID J:135658]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice are bred to wildtype siblings or to CB6F1/J (Stock No. 100007) mice. The donating investigator reports that an increased incidence of hydrocephalus was observed when maintained as coisogneic on a C57BL/6 background. Mating System Noncarrier x Hemizygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $236.40 Female or Male Hemizygous for Tg(Gad1-EGFP)G42Zjh *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $288.65 Hemizygous for Tg(Gad1-EGFP)G42Zjh x Noncarrier $288.65 Noncarrier x Hemizygous for Tg(Gad1-EGFP)G42Zjh
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $307.40 Female or Male Hemizygous for Tg(Gad1-EGFP)G42Zjh *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $375.30 Hemizygous for Tg(Gad1-EGFP)G42Zjh x Noncarrier $375.30 Noncarrier x Hemizygous for Tg(Gad1-EGFP)G42Zjh
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 100007 CB6F1/J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
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