Strain Name: |
CB6-Tg(Gad1-EGFP)G42Zjh/J |
|---|---|
Stock Number: |
007677 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | GFP; Gad1; Tg(Gad1-EGFP)G42Zjh; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Mating System Noncarrier x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Z. Josh Huang, Cold Spring Harbor Laboratory Generation N?+F1 (08-JAN-08) Strain Description
Mice hemizygous for this GAD67-GFP transgene are viable and fertile. GAD67-GFP (line G42) mice selectively express enhanced green fluorescent protein (EGFP) in the calcium-binding protein parvalbumin (Pv)-expressing subclass of basket interneurons (soma, dendrites, and axons) and also in putative presynaptic boutons. While transgene expression (EGFP expression) is published as early as postnatal day 0 (P0) with high expression levels throughout postnatal development, the donating investigator additionally reports transgene expression as early as embryonic day 14 (E14). EGFP expression is not reported in other interneuron classes positive for somatostatin (SOM), cholecystokinin (CCK), calretinin (CR), and VIP. These GAD67-GFP (line G42) mice may be useful for fluorescent labeling of the Pv-expressing subset of GABAergic neurons, allowing reliable and efficient characterization of perisomatic innervation in vivo.Of note, this GAD67-GFP strain is one of many fluorescent GABAergic neuron strains, each with unique labeling characteristics (see Stock No. 003718, Stock No. 006334, Stock No. 006340, Stock No. 007614, and Stock No. 007673).
Strain Development
The bacterial artificial chromosome (BAC) clone 56I19 containing the mouse GAD67 gene (and 60 kb of upstream and downstream sequence) was modified by insertion of an Enhanced Green Fluorescent Protein (EGFP) cDNA and phosphoglycerate kinase polyadenylation sequence in the first coding exon at the translation initiation site of the GAD67 gene. This GAD67-GFP BAC was microinjected into fertilized C57BL/6 mouse eggs. Transgenic offspring with appropriate GFP expression levels and patterns (founder line G42) were established on the C57BL/6 genetic background. After many generations of breeding to C57BL/6, an increase in hydrocephalus incidence was observed on the coisogenic background. To eliminate the undesired phenotype, this colony was subsequently maintained by breeding hemizygous mice to CB6F1/J (Stock No. 100007) for multiple generations prior to arrival at The Jackson Laboratory.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Gad1-EGFP)G42Zjh | ||
|---|---|---|---|
| Allele Name | transgene insertion G42, Z Josh Huang | ||
| Common Name(s) | GAD67-GFP (line G42); | ||
| Strain of Origin | C57BL/6 | ||
| Site of Expression | calcium-binding protein parvalbumin (Pv)-expressing subclass of basket interneurons (soma, dendrites, and axons)and putative presynaptic boutons; detected as early as embryonic day 14 (E14) with high expression levels throughout postnatal development; not reported in other interneuron classes positive for somatostatin (SOM), cholecystokinin (CCK), calretinin (CR), and VIP | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Gad1, glutamic acid decarboxylase 1, mouse, laboratory | ||
| Molecular Note | The bacterial artificial chromosome (BAC) clone 56I19 containing the mouse GAD67 gene (and 60 kb of upstream and downstream sequence) was modified by insertion of an Enhanced Green Fluorescent Protein (EGFP) cDNA and phosphoglycerate kinase polyadenylation sequence in the first coding exon at the translation initiation site of the GAD67 gene. This GAD67-GFP BAC was microinjected into fertilized C57BL/6 mouse eggs. Mice (line G42) selectively express enhanced green fluorescent protein (EGFP) in the calcium-binding protein parvalbumin (Pv)-expressing subclass of basket interneurons (soma, dendrites, and axons) and also in putative presynaptic boutons. Transgene expression (EGFP expression) is detected as early as embryonic day 14 (E14) with high expressionlevels throughout postnatal development. [J:94812] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 100007 CB6F1/J | ||
| Considerations for Choosing Controls | ||
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, hemizygous mice are bred to wildtype siblings or to CB6F1/J (Stock No. 100007) mice. The donating investigator reports that an increased incidence of hydrocephalus was observed when maintained as coisogneic on a C57BL/6 background. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (135 strains)
003718 FVB-Tg(GadGFP)45704Swn/J 006334 STOCK Tg(Gad1-EGFP)94Agmo/J 006340 STOCK Tg(Gad1-EGFP)98Agmo/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
Alzheimer's Disease
Fluorescent protein expression in neural tissue
Parkinson's Disease
Research Tools
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: neurons)
Neurobiology Research (cell marker)
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Chattopadhyaya B; Di Cristo G; Higashiyama H; Knott GW; Kuhlman SJ; Welker E; Huang ZJ. 2004. Experience and activity-dependent maturation of perisomatic GABAergic innervation in primary visual cortex during a postnatal critical period. J Neurosci 24(43):9598-611. [PubMed: 15509747] [J:94812]
Price and Supply Information
| Strain Name: | CB6-Tg(Gad1-EGFP)G42Zjh/J |
| Stock Number: | 007677 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. No age specifications accepted. Colony sized to produce minimal quantities (typically up to 6 mice) upon order receipt; one order per strain. Expected delivery: 1-3 months. Larger quantities or custom orders arranged upon request. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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