| 
| ||||||||||||||||||
| These K14-rtTA mice provide a Tet-On tool that allows the inducible expression of genes in skin cells. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation ?+F1pN1
Generation DefinitionsDonating Investigator Jeffrey Kudlow, University of Alabama at Birmingham Description
Homozygous females and hemizygous males carrying this X-linked K14-rtTA transgene (K14-rtTetA(X) or rtTAX) are viable and fertile. These mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the human keratin 14 promoter. As the transgene is located on the X chromosome, random inactivation of the X chromosome may result in mosaic rtTA expression patterns in female mice. When mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE or tetO), expression of the target gene in the basal cells of the squamous epithelia and in the outer root sheath of the hair follicles is induced with administration of the tetracycline analog, doxycycline (dox). These K14-rtTA mice provide a Tet-On tool that allows the inducible expression of genes in skin cells.Development
The K14-rtTA transgene contains the 2.3 kb fragment of the human keratin 14 promoter that drives expression of the reverse tetracycline transactivator gene (rtTA), followed by an SV40 polyadenylation sequence. The 4.9 kb transgene was injected into one-cell (C57BL/6 x SJL) mouse zygotes. Mice from founder line 208 found to harbor the transgene on the X chromosome (also called founder line K14-X) were maintained on this mixed C57BL/6;SJL background prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| 100012 B6SJLF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of KRT14
006403 129S.B6-Tg(KRT14-Esr1/HRAS)1Pkha/J 006661 129S.B6-Tg(KRT14-RAF1/ESR1)1Pkha/J 004853 B6.C3-Tg(KRT14-Birc5)19Gros/J 009687 B6.Cg-Tg(KRT14-Kitl*)4XTG2Bjl/J 007622 B6;SJL-Tg(KRT14-Hr)551Cct/J 002980 C57BL/6-Tg(Cd152Ig)1Jbs/J 006822 FVB-Tg(KRT14-MAP2K1/Esr1)12Pkha/J 005705 FVB-Tg(KRT14-Vegfa)3Dtm/J 008099 FVB-Tg(KRT14-rtTA)F42Efu/J 017005 FVB.Cg-Tg(KRT14-Fyn*)aJsey/J 004782 STOCK Tg(KRT14-cre)1Amc/J 005107 STOCK Tg(KRT14-cre/ERT)20Efu/J View Strains carrying other alleles of KRT14 (12 strains)
Additional Web Information
Tet Expression Systems
Use of MICE by companies or for-profit entities requires a license prior to shipping.
Use of MICE by companies or for-profit entities requires a license prior to shipping.
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Dermatology Research
Other
Research Tools
Cancer Research
Tetop Tet System
Dermatology Research
Endocrine Deficiency Research
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Tetop Tet System
Internal/Organ Research
Tet Expression Systems
tTA/rtTA Expressing Strains
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(KRT14-rtTA)208Jek | ||
|---|---|---|---|
| Allele Name | transgene insertion 208, Jeffrey E Kudlow | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | K14-X; K14-rtTA; K14-rtTA(X); X-linked K14-rtTA; rtTA(X); | ||
| Mutation Made By | Jeffrey Kudlow, University of Alabama at Birmingham | ||
| Strain of Origin | (C57BL/6 x SJL) | ||
| Site of Expression | Expresses rtTA in skin. | ||
| Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli | ||
| The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). | |||
| Promoter | KRT14, keratin 14, human | ||
| Molecular Note | The K14-rtTA transgene contains the 2.3 kb fragment of the human keratin 14 promoter that drives expression of the reverse tetracycline transactivator gene (rtTA), followed by an SV40 polyadenylation sequence. The 4.9 kb transgene was injected into one-cell (C57BL/6 x SJL) mouse zygotes. Mice from founder line 208 found to harbor the transgene on the X chromosome (also called founder line K14-X). Random X inactivation may result in mosaic rtTA expression patterns in female mice. The transgene when combined with mice carrying a gene under the control of a tetracycline-responsive promoter allows doxycycline-inducible gene expression in skin cells. [MGI Ref ID J:55970] [MGI Ref ID J:96886] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(tTA), Melt Curve Analysis
Tg(tTA), QPCR
Tg(tTA), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Xie W; Chow LT; Paterson AJ; Chin E; Kudlow JE. 1999. Conditional expression of the ErbB2 oncogene elicits reversible hyperplasia in stratified epithelia and up-regulation of TGFalpha expression in transgenic mice. Oncogene 18(24):3593-607. [PubMed: 10380881] [MGI Ref ID J:55970]
Additional References
Tg(KRT14-rtTA)208Jek relatedAdair-Kirk TL; Griffin GL; Meyer MJ; Kelley DG; Miner JH; Keene DR; Marinkovich MP; Ruppert JM; Uitto J; Senior RM. 2012. Keratinocyte-targeted expression of human laminin gamma2 rescues skin blistering and early lethality of laminin gamma2 deficient mice. PLoS One 7(9):e45546. [PubMed: 23029085] [MGI Ref ID J:191995]
Brechbuhl HM; Ghosh M; Smith MK; Smith RW; Li B; Hicks DA; Cole BB; Reynolds PR; Reynolds SD. 2011. beta-Catenin Dosage Is a Critical Determinant of Tracheal Basal Cell Fate Determination. Am J Pathol 179(1):367-79. [PubMed: 21703416] [MGI Ref ID J:173999]
Foster KW; Liu Z; Nail CD; Li X; Fitzgerald TJ; Bailey SK; Frost AR; Louro ID; Townes TM; Paterson AJ; Kudlow JE; Lobo-Ruppert SM; Ruppert JM. 2005. Induction of KLF4 in basal keratinocytes blocks the proliferation-differentiation switch and initiates squamous epithelial dysplasia. Oncogene 24(9):1491-500. [PubMed: 15674344] [MGI Ref ID J:96886]
Li F; Zhou M. 2013. Conditional expression of the dominant-negative TGF-beta receptor type II elicits lingual epithelial hyperplasia in transgenic mice. Dev Dyn 242(5):444-55. [PubMed: 23362225] [MGI Ref ID J:194979]
Li X; Deng W; Nail CD; Bailey SK; Kraus MH; Ruppert JM; Lobo-Ruppert SM. 2006. Snail induction is an early response to Gli1 that determines the efficiency of epithelial transformation. Oncogene 25(4):609-21. [PubMed: 16158046] [MGI Ref ID J:186380]
Lohela M; Helotera H; Haiko P; Dumont DJ; Alitalo K. 2008. Transgenic induction of vascular endothelial growth factor-C is strongly angiogenic in mouse embryos but leads to persistent lymphatic hyperplasia in adult tissues. Am J Pathol 173(6):1891-901. [PubMed: 18988807] [MGI Ref ID J:141410]
Schneider MR; Werner S; Paus R; Wolf E. 2008. Beyond wavy hairs: the epidermal growth factor receptor and its ligands in skin biology and pathology. Am J Pathol 173(1):14-24. [PubMed: 18556782] [MGI Ref ID J:137366]
Smith RW; Hicks DA; Reynolds SD. 2012. Roles for beta-catenin and doxycycline in the regulation of respiratory epithelial cell frequency and function. Am J Respir Cell Mol Biol 46(1):115-24. [PubMed: 21852686] [MGI Ref ID J:191911]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry This transgene is located on the X chromosome. When maintaining a live colony, homozygous females can be bred to hemizygous males.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
|
|
|
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 100012 B6SJLF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
Use restrictions apply, see Policy on Licensing and Use Restrictions.
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.