Strain Name:

B6;SJL-Tg(KRT14-rtTA)208Jek/J

Stock Number:

007678

Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
These K14-rtTA mice provide a Tet-On tool that allows the inducible expression of genes in skin cells.

Description

Strain Information

Type Mutant Stock; Transgenic;
Additional information on Genetically Engineered Mutant Mice.
Mating SystemHemizygote x Noncarrier         (Female x Male)
Specieslaboratory mouse
Generation?+F1 (24-APR-08)
 
Donating Investigator Jeffrey Kudlow,   University of Alabama at Birmingham

Description
Homozygous females and hemizygous males carrying this X-linked K14-rtTA transgene (K14-rtTetA(X) or rtTAX) are viable and fertile. These mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the human keratin 14 promoter. As the transgene is located on the X chromosome, random inactivation of the X chromosome may result in mosaic rtTA expression patterns in female mice. When mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE or tetO), expression of the target gene in the basal cells of the squamous epithelia and in the outer root sheath of the hair follicles is induced with administration of the tetracycline analog, doxycycline (dox). These K14-rtTA mice provide a Tet-On tool that allows the inducible expression of genes in skin cells.

Development
The K14-rtTA transgene contains the 2.3 kb fragment of the human keratin 14 promoter that drives expression of the reverse tetracycline transactivator gene (rtTA), followed by an SV40 polyadenylation sequence. The 4.9 kb transgene was injected into one-cell (C57BL/6 x SJL) mouse zygotes. Mice from founder line 208 found to harbor the transgene on the X chromosome (also called founder line K14-X) were maintained on this mixed C57BL/6;SJL background prior to arrival at The Jackson Laboratory.

Control Information

  Control
   100012 B6SJLF1/J (approximate)
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of KRT14     (10 strains)

View Strains carrying other alleles of rtTA     (17 strains)

Additional Web Information

Tet Expression Systems

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Dermatology Research
Other

Research Tools
Cancer Research (Tetop Tet System)
Dermatology Research
Endocrine Deficiency Research
Genetics Research (Mutagenesis and Transgenesis: Tetop Tet System)
Internal/Organ Research
Tet Expression Systems (tTA/rtTA Expressing Strains)

Genes & Alleles

Gene & Allele Information

Allele Symbol Tg(KRT14-rtTA)208Jek
Allele Name transgene insertion 208, Jeffrey E Kudlow
Allele Type Transgenic (random, expressed)
Common Name(s) K14-X; K14-rtTA; K14-rtTA(X); X-linked K14-rtTA; rtTA(X);
Mutation Made By Jeffrey Kudlow,   University of Alabama at Birmingham
Strain of Origin(C57BL/6 x SJL)
Site of ExpressionExpresses rtTA in skin.
Expressed Gene rtTA, reverse tetracycline-controlled transactivator, E. coli
The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16).
Promoter KRT14, keratin 14, human
Molecular Note The K14-rtTA transgene contains the 2.3 kb fragment of the human keratin 14 promoter that drives expression of the reverse tetracycline transactivator gene (rtTA), followed by an SV40 polyadenylation sequence. The 4.9 kb transgene was injected into one-cell (C57BL/6 x SJL) mouse zygotes. Mice from founder line 208 found to harbor the transgene on the X chromosome (also called founder line K14-X). Random X inactivation may result in mosaic rtTA expression patterns in female mice. The transgene when combined with mice carrying a gene under the control of a tetracycline-responsive promoter allows doxycycline-inducible gene expression in skin cells. [MGI Ref ID J:55970] [MGI Ref ID J:96886]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(tTA), MCA, vers. 4
Tg(tTA), QPCR, vers. 3
Tg(tTA), STD PCR, vers. 2

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Xie W; Chow LT; Paterson AJ; Chin E; Kudlow JE. 1999. Conditional expression of the ErbB2 oncogene elicits reversible hyperplasia in stratified epithelia and up-regulation of TGFalpha expression in transgenic mice. Oncogene 18(24):3593-607. [PubMed: 10380881]  [MGI Ref ID J:55970]

Additional References

Tg(KRT14-rtTA)208Jek related

Foster KW; Liu Z; Nail CD; Li X; Fitzgerald TJ; Bailey SK; Frost AR; Louro ID; Townes TM; Paterson AJ; Kudlow JE; Lobo-Ruppert SM; Ruppert JM. 2005. Induction of KLF4 in basal keratinocytes blocks the proliferation-differentiation switch and initiates squamous epithelial dysplasia. Oncogene 24(9):1491-500. [PubMed: 15674344]  [MGI Ref ID J:96886]

Schneider MR; Werner S; Paus R; Wolf E. 2008. Beyond wavy hairs: the epidermal growth factor receptor and its ligands in skin biology and pathology. Am J Pathol 173(1):14-24. [PubMed: 18556782]  [MGI Ref ID J:137366]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & HusbandryThis transgene is located on the X chromosome. When maintaining a live colony, homozygous females can be bred to hemizygous males.
Mating SystemHemizygote x Noncarrier         (Female x Male)
Diet Information LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice*GenderGenotypes Provided
Individual Mouse Price $236.40Female or MaleHemizygous for Tg(KRT14-rtTA)208Jek
$52.25Female or MaleNoncarrier
Pairs /Price*Pair Genotype
$288.65Hemizygous for Tg(KRT14-rtTA)208Jek x Noncarrier
*Price(s) in US dollars ($)

Additional Supply Details

Supply Notes

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice*GenderGenotypes Provided
Individual Mouse Price $307.40Female or MaleHemizygous for Tg(KRT14-rtTA)208Jek
$68.00Female or MaleNoncarrier
Pairs /Price*Pair Genotype
$375.30Hemizygous for Tg(KRT14-rtTA)208Jek x Noncarrier
*Price(s) in US dollars ($)

Additional Supply Details

Supply Notes

Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes

Control Information

  Control
   100012 B6SJLF1/J (approximate)
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions


See Terms of Use


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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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Contact Information
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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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Terms of Use

Terms of Use


General Terms and Conditions


Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.

For additional Licensing and Use Restrictions view the link(s) below:
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Contact information

General inquiries

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phone:207-288-6470
fax:207-288-6655

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