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| These K14-rtTA mice provide a Tet-On tool that allows the inducible expression of genes in skin cells. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Hemizygote (Female x Male) 24-FEB-09 Species laboratory mouse Generation ?+F2 (01-JAN-09) Donating Investigator Jeffrey Kudlow, University of Alabama at Birmingham Description
Homozygous females and hemizygous males carrying this X-linked K14-rtTA transgene (K14-rtTetA(X) or rtTAX) are viable and fertile. These mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the human keratin 14 promoter. As the transgene is located on the X chromosome, random inactivation of the X chromosome may result in mosaic rtTA expression patterns in female mice. When mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE or tetO), expression of the target gene in the basal cells of the squamous epithelia and in the outer root sheath of the hair follicles is induced with administration of the tetracycline analog, doxycycline (dox). These K14-rtTA mice provide a Tet-On tool that allows the inducible expression of genes in skin cells.Development
The K14-rtTA transgene contains the 2.3 kb fragment of the human keratin 14 promoter that drives expression of the reverse tetracycline transactivator gene (rtTA), followed by an SV40 polyadenylation sequence. The 4.9 kb transgene was injected into one-cell (C57BL/6 x SJL) mouse zygotes. Mice from founder line 208 found to harbor the transgene on the X chromosome (also called founder line K14-X) were maintained on this mixed C57BL/6;SJL background prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| 100012 B6SJLF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of KRT14
006403 129S.B6-Tg(KRT14-Esr1/HRAS)1Pkha/J 006661 129S.B6-Tg(KRT14-RAF1/ESR1)1Pkha/J 004853 B6.C3-Tg(KRT14-Birc5)19Gros/J 007622 B6;SJL-Tg(KRT14-Hr)551Cct/J 002980 C57BL/6-Tg(Cd152Ig)1Jbs/J 006822 FVB-Tg(KRT14-MAP2K1/Esr1)12Pkha/J 005705 FVB-Tg(KRT14-Vegfa)3Dtm/J 008099 FVB-Tg(KRT14-rtTA)F42Efu/J 004782 STOCK Tg(KRT14-cre)1Amc/J 005107 STOCK Tg(KRT14-cre/Esr1)20Efu/J View Strains carrying other alleles of KRT14 (10 strains)
006965 B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae/J 005670 B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J 007176 B6.Cg-Tg(Pax8-rtTA2S*M2)1Koes/J 006235 B6.Cg-Tg(SFTPC-rtTA)5Jaw/J 006232 B6.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 006911 B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm2(tetO-Pou5f1)Jae/J 010576 B6;SJL-Tg(MMTV-rtTA)4-1Jek/J 006245 C.Cg-Tg(SFTPC-rtTA)5Jaw/J 006242 C.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 008099 FVB-Tg(KRT14-rtTA)F42Efu/J 004127 FVB-Tg(Nes-rtTA)306Rvs/J 006225 FVB.Cg-Tg(SFTPC-rtTA)5Jaw/J 006222 FVB.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 008202 FVB/N-Tg(NPHS2-rtTA2*M2)1Jbk/J 006875 FVB/N-Tg(Tagln-rtTA)E1Jwst/J 004602 NOD.Cg-Tg(Ins2-rtTA)2Doi/DoiJ 005734 NOD/Lt-Tg(Ins2-rtTA)1Ach/AchJ 005572 STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J 008755 STOCK Tg(Ins2-rtTA)2Efr Tg(teto-DTA)1Gfi/J 008250 STOCK Tg(Ins2-rtTA)2Efr/J 005493 STOCK Tg(Tek-rtTA,TRE-lacZ)1425Tpr/J 003273 STOCK Tg(rtTAhCMV)4Bjd/J
Additional Web Information
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Dermatology Research
Other
Research Tools
Cancer Research
Tetop Tet System
Dermatology Research
Endocrine Deficiency Research
Genetics Research
Mutagenesis and Transgenesis: Tetop Tet System
Internal/Organ Research
Tet Expression Systems
tTA/rtTA Expressing Strains
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(KRT14-rtTA)208Jek | ||
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| Allele Name | transgene insertion 208, Jeffrey E Kudlow | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | K14-X; K14-rtTA; K14-rtTA(X); X-linked K14-rtTA; rtTA(X); | ||
| Mutation Made By | Jeffrey Kudlow, University of Alabama at Birmingham | ||
| Strain of Origin | (C57BL/6 x SJL) | ||
| Site of Expression | Expresses rtTA in skin. | ||
| Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli | ||
| The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). | |||
| Promoter | KRT14, keratin 14, human | ||
| Molecular Note | The K14-rtTA transgene contains the 2.3 kb fragment of the human keratin 14 promoter that drives expression of the reverse tetracycline transactivator gene (rtTA), followed by an SV40 polyadenylation sequence. The 4.9 kb transgene was injected into one-cell (C57BL/6 x SJL) mouse zygotes. Mice from founder line 208 found to harbor the transgene on the X chromosome (also called founder line K14-X). Random X inactivation may result in mosaic rtTA expression patterns in female mice. The transgene when combined with mice carrying a gene under the control of a tetracycline-responsive promoter allows doxycycline-inducible gene expression in skin cells. [MGI Ref ID J:55970] [MGI Ref ID J:96886] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(tTA), Melt Curve Analysis
Tg(tTA), QPCR
Tg(tTA), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Xie W; Chow LT; Paterson AJ; Chin E; Kudlow JE. 1999. Conditional expression of the ErbB2 oncogene elicits reversible hyperplasia in stratified epithelia and up-regulation of TGFalpha expression in transgenic mice. Oncogene 18(24):3593-607. [PubMed: 10380881] [MGI Ref ID J:55970]
Additional References
Tg(KRT14-rtTA)208Jek relatedFoster KW; Liu Z; Nail CD; Li X; Fitzgerald TJ; Bailey SK; Frost AR; Louro ID; Townes TM; Paterson AJ; Kudlow JE; Lobo-Ruppert SM; Ruppert JM. 2005. Induction of KLF4 in basal keratinocytes blocks the proliferation-differentiation switch and initiates squamous epithelial dysplasia. Oncogene 24(9):1491-500. [PubMed: 15674344] [MGI Ref ID J:96886]
Lohela M; Helotera H; Haiko P; Dumont DJ; Alitalo K. 2008. Transgenic induction of vascular endothelial growth factor-C is strongly angiogenic in mouse embryos but leads to persistent lymphatic hyperplasia in adult tissues. Am J Pathol 173(6):1891-901. [PubMed: 18988807] [MGI Ref ID J:141410]
Schneider MR; Werner S; Paus R; Wolf E. 2008. Beyond wavy hairs: the epidermal growth factor receptor and its ligands in skin biology and pathology. Am J Pathol 173(1):14-24. [PubMed: 18556782] [MGI Ref ID J:137366]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry This transgene is located on the X chromosome. When maintaining a live colony, homozygous females can be bred to hemizygous males. Mating System Homozygote x Hemizygote (Female x Male) 24-FEB-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $209.90 Male Hemizygous for Tg(KRT14-rtTA)208Jek $209.90 Female Homozygous for Tg(KRT14-rtTA)208Jek
Pairs /Price (US dollars $) Pair Genotype $419.80 Homozygous for Tg(KRT14-rtTA)208Jek x Hemizygous for Tg(KRT14-rtTA)208Jek
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $272.90 Male Hemizygous for Tg(KRT14-rtTA)208Jek $272.90 Female Homozygous for Tg(KRT14-rtTA)208Jek
Pairs /Price (US dollars $) Pair Genotype $545.80 Homozygous for Tg(KRT14-rtTA)208Jek x Hemizygous for Tg(KRT14-rtTA)208Jek
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 100012 B6SJLF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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General Terms and Conditions
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
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