Strain Name:

B6;SJL-Tg(KRT14-rtTA)208Jek/J

Stock Number:

007678

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
These K14-rtTA mice provide a Tet-On tool that allows the inducible expression of genes in skin cells.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
Generation?+F1pN1
Generation Definitions
 
Donating Investigator Jeffrey Kudlow,   University of Alabama at Birmingham

Description
Homozygous females and hemizygous males carrying this X-linked K14-rtTA transgene (K14-rtTetA(X) or rtTAX) are viable and fertile. These mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the human keratin 14 promoter. As the transgene is located on the X chromosome, random inactivation of the X chromosome may result in mosaic rtTA expression patterns in female mice. When mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE or tetO), expression of the target gene in the basal cells of the squamous epithelia and in the outer root sheath of the hair follicles is induced with administration of the tetracycline analog, doxycycline (dox). These K14-rtTA mice provide a Tet-On tool that allows the inducible expression of genes in skin cells.

Development
The K14-rtTA transgene contains the 2.3 kb fragment of the human keratin 14 promoter that drives expression of the reverse tetracycline transactivator gene (rtTA), followed by an SV40 polyadenylation sequence. The 4.9 kb transgene was injected into one-cell (C57BL/6 x SJL) mouse zygotes. Mice from founder line 208 found to harbor the transgene on the X chromosome (also called founder line K14-X) were maintained on this mixed C57BL/6;SJL background prior to arrival at The Jackson Laboratory.

Control Information

  Control
   100012 B6SJLF1/J (approximate)
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of KRT14     (13 strains)

Strains carrying other alleles of rtTA
016567   129S.Cg-Tg(Hoxb7-rtTA*M2)2Cos/J
017983   B6.Cg-Col1a1tm9(tetO-Dnmt3b_i1)Jae Gt(ROSA)26Sortm1(rtTA*M2)Jae/J
014588   B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm6(tetO-MSI2)Jae/J
014602   B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm1(tetO-mCherry)Eggn/J
023749   B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae Tg(tetO-Pou5f1,-Sox2,-Klf4,-Myc)1Srn/J
006965   B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae/J
005670   B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
016997   B6.Cg-Tg(Axin2-rtTA2S*M2)7Cos/J
012418   B6.Cg-Tg(CD68-rtTA2S*M2)3Mpil/Mmjax
014098   B6.Cg-Tg(GFAP-rtTA*M2)1Rmra/J
007176   B6.Cg-Tg(Pax8-rtTA2S*M2)1Koes/J
006235   B6.Cg-Tg(SFTPC-rtTA)5Jaw/J
006232   B6.Cg-Tg(Scgb1a1-rtTA)1Jaw/J
021025   B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm1(tetO-cre)Haho/J
006911   B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm2(tetO-Pou5f1)Jae/J
016836   B6;129S4-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm7(tetO-HIST1H2BJ/GFP)Jae/J
012433   B6;C3-Tg(ACTA1-rtTA,tetO-cre)102Monk/J
021187   B6;FVB-Tg(Pbsn-rtTA*M2)42Xy/J
010574   B6;SJL-Tg(Gh1-rtTA)4-3Jek/J
010576   B6;SJL-Tg(MMTV-rtTA)4-1Jek/J
010549   B6N.Cg-Tg(Prkcd-glc-1-rtTA)2And/J
016532   B6N.FVB(Cg)-Tg(CAG-rtTA3)4288Slowe/J
006245   C.Cg-Tg(SFTPC-rtTA)5Jaw/J
006242   C.Cg-Tg(Scgb1a1-rtTA)1Jaw/J
017955   C57BL/6-Tg(Gfap-rtTA,tetO-MAOB,-lacZ)1Jkan/J
008099   FVB-Tg(KRT14-rtTA)F42Efu/J
004127   FVB-Tg(Nes-rtTA)306Rvs/J
008326   FVB-Tg(Pomc-rtTA)1Rck/J
006225   FVB.Cg-Tg(SFTPC-rtTA)5Jaw/J
006222   FVB.Cg-Tg(Scgb1a1-rtTA)1Jaw/J
008202   FVB/N-Tg(NPHS2-rtTA2*M2)1Jbk/J
006875   FVB/N-Tg(Tagln-rtTA)E1Jwst/J
004602   NOD.Cg-Tg(Ins2-rtTA)2Doi/DoiJ
005734   NOD/Lt-Tg(Ins2-rtTA)1Ach/AchJ
011004   STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm3(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae/J
011011   STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm4(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae/J
011013   STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm5(tetO-Pou5f1,-Klf4,-Myc)Jae/J
005572   STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
016116   STOCK Waptm2(rtTA)Kuw/J
003273   STOCK Tg(CMV-rtTA)4Bjd/J
018156   STOCK Tg(Drd1a-rtTA)ARgmk/J
019110   STOCK Tg(Hoxb7-rtTA*M2)RS40BCos/Mmjax
008755   STOCK Tg(Ins2-rtTA)2Efr Tg(teto-DTA)1Gfi/J
008250   STOCK Tg(Ins2-rtTA)2Efr/J
017519   STOCK Tg(KRT5-rtTA)T2D6Sgkd/J
016146   STOCK Tg(SFTPC-rtTA)2Jaw/J
016145   STOCK Tg(Scgb1a1-rtTA)2Jaw/J
005493   STOCK Tg(Tek-rtTA,TRE-lacZ)1425Tpr/J
View Strains carrying other alleles of rtTA     (48 strains)

Additional Web Information

Tet Expression Systems

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Dermatology Research
Other

Research Tools
Cancer Research
      Tetop Tet System
Dermatology Research
Endocrine Deficiency Research
Genetics Research
      Mutagenesis and Transgenesis
      Mutagenesis and Transgenesis: Tetop Tet System
Internal/Organ Research
Tet Expression Systems
      tTA/rtTA Expressing Strains

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(KRT14-rtTA)208Jek
Allele Name transgene insertion 208, Jeffrey E Kudlow
Allele Type Transgenic (Inserted expressed sequence)
Common Name(s) K14-X; K14-rtTA; K14-rtTA(X); X-linked K14-rtTA; rtTA(X);
Mutation Made By Jeffrey Kudlow,   University of Alabama at Birmingham
Strain of Origin(C57BL/6 x SJL)
Site of ExpressionExpresses rtTA in skin.
Expressed Gene rtTA, reverse tetracycline-controlled transactivator, E. coli
The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16).
Promoter KRT14, keratin 14, human
Molecular Note The K14-rtTA transgene contains the 2.3 kb fragment of the human keratin 14 promoter that drives expression of the reverse tetracycline transactivator gene (rtTA), followed by an SV40 polyadenylation sequence. The 4.9 kb transgene was injected into one-cell (C57BL/6 x SJL) mouse zygotes. Mice from founder line 208 found to harbor the transgene on the X chromosome (also called founder line K14-X). Random X inactivation may result in mosaic rtTA expression patterns in female mice. The transgene when combined with mice carrying a gene under the control of a tetracycline-responsive promoter allows doxycycline-inducible gene expression in skin cells. [MGI Ref ID J:55970] [MGI Ref ID J:96886]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(tTA),

MELT


Tg(tTA),

Probe


Tg(tTA), QPCR
Tg(tTA), Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Xie W; Chow LT; Paterson AJ; Chin E; Kudlow JE. 1999. Conditional expression of the ErbB2 oncogene elicits reversible hyperplasia in stratified epithelia and up-regulation of TGFalpha expression in transgenic mice. Oncogene 18(24):3593-607. [PubMed: 10380881]  [MGI Ref ID J:55970]

Additional References

Tg(KRT14-rtTA)208Jek related

Adair-Kirk TL; Griffin GL; Meyer MJ; Kelley DG; Miner JH; Keene DR; Marinkovich MP; Ruppert JM; Uitto J; Senior RM. 2012. Keratinocyte-targeted expression of human laminin gamma2 rescues skin blistering and early lethality of laminin gamma2 deficient mice. PLoS One 7(9):e45546. [PubMed: 23029085]  [MGI Ref ID J:191995]

Brechbuhl HM; Ghosh M; Smith MK; Smith RW; Li B; Hicks DA; Cole BB; Reynolds PR; Reynolds SD. 2011. beta-Catenin Dosage Is a Critical Determinant of Tracheal Basal Cell Fate Determination. Am J Pathol 179(1):367-79. [PubMed: 21703416]  [MGI Ref ID J:173999]

Foster KW; Liu Z; Nail CD; Li X; Fitzgerald TJ; Bailey SK; Frost AR; Louro ID; Townes TM; Paterson AJ; Kudlow JE; Lobo-Ruppert SM; Ruppert JM. 2005. Induction of KLF4 in basal keratinocytes blocks the proliferation-differentiation switch and initiates squamous epithelial dysplasia. Oncogene 24(9):1491-500. [PubMed: 15674344]  [MGI Ref ID J:96886]

Li F; Zhou M. 2013. Conditional expression of the dominant-negative TGF-beta receptor type II elicits lingual epithelial hyperplasia in transgenic mice. Dev Dyn 242(5):444-55. [PubMed: 23362225]  [MGI Ref ID J:194979]

Li X; Deng W; Nail CD; Bailey SK; Kraus MH; Ruppert JM; Lobo-Ruppert SM. 2006. Snail induction is an early response to Gli1 that determines the efficiency of epithelial transformation. Oncogene 25(4):609-21. [PubMed: 16158046]  [MGI Ref ID J:186380]

Lohela M; Helotera H; Haiko P; Dumont DJ; Alitalo K. 2008. Transgenic induction of vascular endothelial growth factor-C is strongly angiogenic in mouse embryos but leads to persistent lymphatic hyperplasia in adult tissues. Am J Pathol 173(6):1891-901. [PubMed: 18988807]  [MGI Ref ID J:141410]

Schneider MR; Werner S; Paus R; Wolf E. 2008. Beyond wavy hairs: the epidermal growth factor receptor and its ligands in skin biology and pathology. Am J Pathol 173(1):14-24. [PubMed: 18556782]  [MGI Ref ID J:137366]

Smith RW; Hicks DA; Reynolds SD. 2012. Roles for beta-catenin and doxycycline in the regulation of respiratory epithelial cell frequency and function. Am J Respir Cell Mol Biol 46(1):115-24. [PubMed: 21852686]  [MGI Ref ID J:191911]

Zhao H; Feng J; Seidel K; Shi S; Klein O; Sharpe P; Chai Y. 2014. Secretion of shh by a neurovascular bundle niche supports mesenchymal stem cell homeostasis in the adult mouse incisor. Cell Stem Cell 14(2):160-73. [PubMed: 24506883]  [MGI Ref ID J:210170]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryThis transgene is located on the X chromosome. When maintaining a live colony, homozygous females can be bred to hemizygous males.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   100012 B6SJLF1/J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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