Strain Name: |
STOCK Epb4.1l3tm1Jkis/J |
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Stock Number: |
007681 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Epb4.1l3; Epb4.1l3tm1Jkis; |
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System Heterozygote x Heterozygote (Female x Male) Species laboratory mouse Donating Investigator Joseph Kissil, Wistar Institute Strain Description
Mice homozygous for this 4.1B/Dal-1 targeted allele are viable, fertile, and age normally with no spontaneous tumor formation. Lung, brain, breast, and prostate tissues from homozygotes show no protein expression from the mutant locus. Although 4.1B-deficient females do not form tumors of the mammary gland, a significant increase in mammary epithelial cell proliferation during pregnancy is observed. When these 4.1B-deficient mice are bred with TRAMP transgenic mice (see Sock No. 003135), the resulting double mutant offspring exhibit increased susceptibility for developing aggressive prostate carcinomas and enhanced tumor malignancy associated with reduced apoptosis. Because 4.1B expression is frequently downregulated in human clinical prostate cancer (and a spectrum of other tumor types), these 4.1B mutant mice may be useful in studying the role of 4.1B as a negative regulator of cancer progression to metastatic disease.Strain Development
A targeting vector was designed to replace the 3' region of exon 3 (which is the second coding exon of the 4.1B locus and contains the initiating codon of the Dal-1 isoform) of the targeted gene with a neomycin selection cassette. The construct was electroporated into the 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting mutant mice were maintained on a mixed ("129/SvJae and C57BL/6 and possibly other") genetic background prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J for at least one generation to establish the colony.
Mammalian Phenotype Terms assigned by genotype |
| Allele Symbol | Epb4.1l3tm1Jkis | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Joseph L Kissil | ||
| Common Name(s) | 4.1B-; | ||
| Strain of Origin | 129S4/SvJae | ||
| ES Cell Line Name | J1 | ||
| ES Cell Line Strain | 129S4/SvJae | ||
| Gene Symbol and Name | Epb4.1l3, erythrocyte protein band 4.1-like 3 | ||
| Chromosome | 17 | ||
| Gene Common Name(s) | 4.1B; DAL-1; DAL1; DAL1P; Epb41l3; FLJ37633; KIAA0987; NBL3; | ||
| Molecular Note | A neomycin resistance cassette replaced most of the 3' sequence in exon 3, which is the second coding exon of the 4.1B locus and contains the initiating codon of the Dal-1 isoform. Western blot failed to detect protein in mutant mice. [MGI Ref ID J:102380] | ||
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
| Breeding & Husbandry | When maintained as a live colony, homozygous mice may be bred together. |
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Room Number AX11
Cancer Research
Genes Regulating Growth and Proliferation
Other (tumor metastasis)
Tumor Resistance
Tumor Suppressor Genes
Research Tools
Cancer Research
Selected Reference(s)
Additional ReferencesWong SY; Haack H; Kissil JL; Barry M; Bronson RT; Shen SS; Whittaker CA; Crowley D; Hynes RO. 2007. Protein 4.1B suppresses prostate cancer progression and metastasis. Proc Natl Acad Sci U S A 104(31):12784-9. [PubMed: 17640904] [MGI Ref ID J:123122]
Yi C; McCarty JH; Troutman SA; Eckman MS; Bronson RT; Kissil JL. 2005. Loss of the putative tumor suppressor band 4.1B/Dal1 gene is dispensable for normal development and does not predispose to cancer. Mol Cell Biol 25(22):10052-9. [PubMed: 16260618] [MGI Ref ID J:102380]
| Strain Name: | STOCK Epb4.1l3tm1Jkis/J |
| Stock Number: | 007681 |
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
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