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Genes & Alleles

Epb4.1l3;   Epb4.1l3tm1Jkis;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System Heterozygote x Heterozygote         (Female x Male) Species laboratory mouse Donating Investigator Joseph Kissil,   Wistar Institute

Strain Description
Mice homozygous for this 4.1B/Dal-1 targeted allele are viable, fertile, and age normally with no spontaneous tumor formation. Lung, brain, breast, and prostate tissues from homozygotes show no protein expression from the mutant locus. Although 4.1B-deficient females do not form tumors of the mammary gland, a significant increase in mammary epithelial cell proliferation during pregnancy is observed. When these 4.1B-deficient mice are bred with TRAMP transgenic mice (see Sock No. 003135), the resulting double mutant offspring exhibit increased susceptibility for developing aggressive prostate carcinomas and enhanced tumor malignancy associated with reduced apoptosis. Because 4.1B expression is frequently downregulated in human clinical prostate cancer (and a spectrum of other tumor types), these 4.1B mutant mice may be useful in studying the role of 4.1B as a negative regulator of cancer progression to metastatic disease.

Strain Development
A targeting vector was designed to replace the 3' region of exon 3 (which is the second coding exon of the 4.1B locus and contains the initiating codon of the Dal-1 isoform) of the targeted gene with a neomycin selection cassette. The construct was electroporated into the 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting mutant mice were maintained on a mixed ("129/SvJae and C57BL/6 and possibly other") genetic background prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J for at least one generation to establish the colony.

Mammalian Phenotype Terms assigned by genotype The following phenotype information may relate to a genetic background differing from this JAX® Mice strain. Epb4.1l3tm1Jkis/Epb4.1l3tm1Jkis         involves: 129S4/SvJae normal phenotype no abnormal phenotype detected (MGI Ref ID J:102380) homozygous null mice develop normally, are fertile, do not develop tumors and show no differences in cellular proliferation and apoptosis

Gene & Allele Details

Allele Symbol		Epb4.1l3tm1Jkis
Allele Name		targeted mutation 1, Joseph L Kissil
Common Name(s)		4.1B-;
Strain of Origin		129S4/SvJae
ES Cell Line Name		J1
ES Cell Line Strain		129S4/SvJae
Gene Symbol and Name		Epb4.1l3, erythrocyte protein band 4.1-like 3
Chromosome		17
Gene Common Name(s)		4.1B; DAL-1; DAL1; DAL1P; Epb41l3; FLJ37633; KIAA0987; NBL3;
Molecular Note		A neomycin resistance cassette replaced most of the 3' sequence in exon 3, which is the second coding exon of the 4.1B locus and contains the initiating codon of the Dal-1 isoform. Western blot failed to detect protein in mutant mice. [MGI Ref ID J:102380]

Control Information

	Control
	Wild-type from the colony
	101043 B6129SF1/J	(approximate)
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls

Colony Maintenance

Breeding & Husbandry	When maintained as a live colony, homozygous mice may be bred together.

Animal Health Reports

Room Number           AX11

Research Applications

This mouse can be used to support research in many areas including:

Cancer Research
Genes Regulating Growth and Proliferation
Other (tumor metastasis)
Tumor Resistance
Tumor Suppressor Genes

Research Tools
Cancer Research

References

Selected Reference(s)

Wong SY; Haack H; Kissil JL; Barry M; Bronson RT; Shen SS; Whittaker CA; Crowley D; Hynes RO. 2007. Protein 4.1B suppresses prostate cancer progression and metastasis. Proc Natl Acad Sci U S A 104(31):12784-9. [PubMed: 17640904]  [MGI Ref ID J:123122]

Yi C; McCarty JH; Troutman SA; Eckman MS; Bronson RT; Kissil JL. 2005. Loss of the putative tumor suppressor band 4.1B/Dal1 gene is dispensable for normal development and does not predispose to cancer. Mol Cell Biol 25(22):10052-9. [PubMed: 16260618]  [MGI Ref ID J:102380]

Additional References

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Price and Supply Information

Strain Name:	STOCK Epb4.1l3tm1Jkis/J
Stock Number:	007681

Price Details

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Supply Details

Standard Supply	Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes	Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.
Licensing	See General Terms and Conditions below
Control Information	View Control Information in Strain Details.

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View JAX^® Mice & Services Conditions of Use.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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