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| Because 4.1B expression is frequently downregulated in human clinical prostate cancer (and a spectrum of other tumor types), these 4.1B mutant mice may be useful in studying the role of 4.1B as a negative regulator of cancer progression to metastatic disease. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N?pN1
Generation DefinitionsDonating Investigator Joseph L Kissil, Wistar Institute Description
Mice homozygous for this 4.1B/Dal-1 targeted allele are viable, fertile, and age normally with no spontaneous tumor formation. Lung, brain, breast, and prostate tissues from homozygotes show no protein expression from the mutant locus. Although 4.1B-deficient females do not form tumors of the mammary gland, a significant increase in mammary epithelial cell proliferation during pregnancy is observed. When these 4.1B-deficient mice are bred with TRAMP transgenic mice (see Sock No. 003135), the resulting double mutant offspring exhibit increased susceptibility for developing aggressive prostate carcinomas and enhanced tumor malignancy associated with reduced apoptosis. Because 4.1B expression is frequently downregulated in human clinical prostate cancer (and a spectrum of other tumor types), these 4.1B mutant mice may be useful in studying the role of 4.1B as a negative regulator of cancer progression to metastatic disease.Development
A targeting vector was designed to replace the 3' region of exon 3 (which is the second coding exon of the 4.1B locus and contains the initiating codon of the Dal-1 isoform) of the targeted gene with a neomycin selection cassette. The construct was electroporated into the 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting mutant mice were maintained on a mixed ("129/SvJae and C57BL/6 and possibly other") genetic background prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
Phenotype
Phenotype Information
View Mammalian Phenotype Terms
Mammalian Phenotype Terms provided by MGI
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Epb4.1l3tm1Jkis/Epb4.1l3tm1Jkis
involves: 129S4/SvJae
- cellular phenotype
- *normal* cellular phenotype
- no alteration in cellular proliferation and apoptosis (MGI Ref ID J:102380)
- reproductive system phenotype
- *normal* reproductive system phenotype
- homozygous mice are fertile (MGI Ref ID J:102380)
- tumorigenesis
- *normal* tumorigenesis
- mice show no tendancy to increased tumor development (MGI Ref ID J:102380)
- nervous system phenotype
- abnormal paranodal axoglial junction morphology
- septate junction septa in sciatic nerves are normal at 3 months (MGI Ref ID J:173383)
- septate junction septa in sciatic nerves are missing at 6 and 12 months of age (MGI Ref ID J:173383)
- axolemma is essentially detached from myelin loops in the sciatic nerve at 1 year of age, no septa (MGI Ref ID J:173383)
This mouse can be used to support research in many areas including:
Cancer Research
Genes Regulating Growth and Proliferation
Other
tumor metastasis
Tumor Resistance
Tumor Suppressor Genes
Research Tools
Cancer Research
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Epb4.1l3tm1Jkis | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Joseph L Kissil | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | 4.1B-; | ||
| Mutation Made By | Joseph Kissil, Wistar Institute | ||
| Strain of Origin | 129S4/SvJae | ||
| ES Cell Line Name | J1 | ||
| ES Cell Line Strain | 129S4/SvJae | ||
| Gene Symbol and Name | Epb4.1l3, erythrocyte protein band 4.1-like 3 | ||
| Chromosome | 17 | ||
| Gene Common Name(s) | 4.1B; DAL-1; DAL1; DAL1P; Kiaa0987; NBL3; | ||
| Molecular Note | A neomycin resistance cassette replaced most of the 3' sequence in exon 3, which is the second coding exon of the 4.1B locus and contains the initiating codon of the Dal-1 isoform. Western blot failed to detect protein in mutant mice. [MGI Ref ID J:102380] | ||
References
References provided by MGI
Selected Reference(s)
Wong SY; Haack H; Kissil JL; Barry M; Bronson RT; Shen SS; Whittaker CA; Crowley D; Hynes RO. 2007. Protein 4.1B suppresses prostate cancer progression and metastasis. Proc Natl Acad Sci U S A 104(31):12784-9. [PubMed: 17640904] [MGI Ref ID J:123122]
Yi C; McCarty JH; Troutman SA; Eckman MS; Bronson RT; Kissil JL. 2005. Loss of the putative tumor suppressor band 4.1B/Dal1 gene is dispensable for normal development and does not predispose to cancer. Mol Cell Biol 25(22):10052-9. [PubMed: 16260618] [MGI Ref ID J:102380]
Additional References
Epb4.1l3tm1Jkis relatedButtermore ED; Dupree JL; Cheng J; An X; Tessarollo L; Bhat MA. 2011. The Cytoskeletal Adaptor Protein Band 4.1B Is Required for the Maintenance of Paranodal Axoglial Septate Junctions in Myelinated Axons. J Neurosci 31(22):8013-8024. [PubMed: 21632923] [MGI Ref ID J:173383]
Einheber S; Meng X; Rubin M; Lam I; Mohandas N; An X; Shrager P; Kissil J; Maurel P; Salzer JL. 2013. The 4.1B cytoskeletal protein regulates the domain organization and sheath thickness of myelinated axons. Glia 61(2):240-53. [PubMed: 23109359] [MGI Ref ID J:191117]
Horresh I; Bar V; Kissil JL; Peles E. 2010. Organization of myelinated axons by Caspr and Caspr2 requires the cytoskeletal adapter protein 4.1B. J Neurosci 30(7):2480-9. [PubMed: 20164332] [MGI Ref ID J:157838]
Jung Y; Kissil JL; McCarty JH. 2011. beta8 integrin and band 4.1B cooperatively regulate morphogenesis of the embryonic heart. Dev Dyn 240(1):271-7. [PubMed: 21181944] [MGI Ref ID J:167708]
Jung Y; McCarty JH. 2012. Band 4.1 proteins regulate integrin-dependent cell spreading. Biochem Biophys Res Commun 426(4):578-84. [PubMed: 22982319] [MGI Ref ID J:190121]
Kuns R; Kissil JL; Newsham IF; Jacks T; Gutmann DH; Sherman LS. 2005. Protein 4.1B expression is induced in mammary epithelial cells during pregnancy and regulates their proliferation. Oncogene 24(43):6502-15. [PubMed: 16007173] [MGI Ref ID J:101760]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintained as a live colony, homozygous mice may be bred together.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2085.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2710.50 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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