Strain Name: |
STOCK Tg(Atoh1-cre/ESR1)14Fsh/J |
|---|---|
Stock Number: |
007684 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Atoh1; ESR1; cre; Tg(Atoh1-cre/ESR1)14Fsh; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Mating System Noncarrier x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Gord Fishell, The Skirball Institute, NYU Sch. of Med. Strain Description
Mice hemizygous for this Math1-CreERT2 transgene are viable and fertile. Under control of the Math1 (Atoh1) enhancer, tamoxifen-inducible cre activity is observed in neural progenitors of the cerebellar rhombic lip, dorsal hindbrain and spinal cord, as well as in inner-ear primordia (with a limited amount of ectopic expression in the primordium of the hippocampus but not the cortex). The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17b-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered. When these Math1-CreERT2 mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in Math1 expressing cells (the entire dorsal neural tube from posterior rhombomere 1 to the spinal cord); making them useful in studying cerebellum development and fate mapping of Math1/Atoh1 cells.Strain Development
The Math1-CreERT2 transgene was designed with a 1.4 kb mouse Math1 (Atoh1) 3' enhancer (and a b-globin minimal promoter) sequence upstream of a CreERT2 fusion gene (Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain). The transgene was microinjected into FVB/N embryos. Founder mice were bred to mice on a mixed outbred background to test transgene efficacy. Mice found to exhibit appropriate expression levels and patterns (founder line 14) were selected and bred to Swiss Webster outbred mice. Transgenic mice were bred to Swiss Webster for several generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to FVB/NJ for at least one generation to establish the colony.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Atoh1-cre/ESR1)14Fsh | ||
|---|---|---|---|
| Allele Name | transgene insertion 14, Gordon Fishell | ||
| Common Name(s) | Math1-CreERT2; Tg(Atoh1-cre/ESR1)1Fsh; Tg(HBB-cre)1Fsh; | ||
| Mutation Made By | Rob Machold, The Skirball Institute, NYU Sch. of Med. | ||
| Strain of Origin | FVB/N | ||
| Site of Expression | tamoxifen-inducible cre activity is observed in neural progenitors of the cerebellar rhombic lip, dorsal hindbrain and spinal cord, as well as in inner-ear primordia (with a limited amount of ectopic expression in the primordium of the hippocampus but not the cortex) | ||
| Expressed Gene | ESR1, estrogen receptor 1, human | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Atoh1, atonal homolog 1 (Drosophila), mouse, laboratory | ||
| Molecular Note | The transgene was designed with a 1.4 kb mouse Math1 (Atoh1) 3' enhancer (and a beta-globin minimal promoter) sequence upstream of a CreERT2 fusion gene (Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain). Tamoxifen-inducible cre activity is observed in neural progenitors of the cerebellar rhombic lip, dorsal hindbrain and spinal cord, as well as in inner-ear primordia (with a limited amount of ectopic expression in the primordium of the hippocampus but not the cortex). [MGI Ref ID J:105186] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, transgenic mice can be bred to wildtype siblings or bred together, although a homozygous phenotype is not yet characterized (Aug-2007). |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Atoh1
005970 B6.129S7-Atoh1tm2Hzo/J View Strains carrying other alleles of Atoh1 (1 strain)
Additional Web Information
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
cre relatedNeurobiology Research
Cerebellar Defects
Research Tools
Cre-lox System (Cre-Recombinase Expression: Inducible)
Developmental Biology Research (Cre-lox System)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genetics Research (Tissue/Cell Markers: Cre-lox System)
Genetics Research (Tissue/Cell Markers: neurons)
Neurobiology Research (cell marker)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Additional ReferencesMachold R; Fishell G. 2005. Math1 is expressed in temporally discrete pools of cerebellar rhombic-lip neural progenitors. Neuron 48(1):17-24. [PubMed: 16202705] [MGI Ref ID J:105186]
Price and Supply Information
| Strain Name: | STOCK Tg(Atoh1-cre/ESR1)14Fsh/J |
| Stock Number: | 007684 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Notice Regarding cre/Esr1 (Tamoxifen Inducible Cre) Strains.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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