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| When these Math1-CreERT2 mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in Math1 expressing cells (the entire dorsal neural tube from posterior rhombomere 1 to the spinal cord); making them useful in studying cerebellum development and fate mapping of Math1/Atoh1 cells. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Former Names STOCK Tg(Atoh1-cre/ESR1)14Fsh/J (Changed: 01-APR-10 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Noncarrier x Hemizygote (Female x Male) 10-APR-08 Species laboratory mouse Generation ?+F2 (01-JAN-09)
Generation DefinitionsDonating Investigator Gord Fishell, NYU School of Medicine Description
Mice hemizygous for this Math1-CreERT2 transgene are viable and fertile. Under control of the Math1 (Atoh1) enhancer, tamoxifen-inducible cre activity is observed in neural progenitors of the cerebellar rhombic lip, dorsal hindbrain and spinal cord, as well as in inner-ear primordia (with a limited amount of ectopic expression in the primordium of the hippocampus but not the cortex). The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor; which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered. When these Math1-CreERT2 mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in Math1 expressing cells (the entire dorsal neural tube from posterior rhombomere 1 to the spinal cord); making them useful in studying cerebellum development and fate mapping of Math1/Atoh1 cells.Development
The Math1-CreERT2 transgene was designed with a 1.4 kb mouse Math1 (Atoh1) 3' enhancer (and a β-globin minimal promoter) sequence upstream of a CreERT2 fusion gene (Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain). The transgene was microinjected into FVB/N embryos. Founder mice were bred to mice on a mixed outbred background to test transgene efficacy. Mice found to exhibit appropriate expression levels and patterns (founder line 14) were selected and bred to Swiss Webster outbred mice. Transgenic mice were bred to Swiss Webster for several generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred to FVB/NJ for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Atoh1
013593 B6.129S-Atoh1tm4.1Hzo/J 013594 B6.129S-Atoh1tm5.1(Cre/PGR)Hzo/J 013595 B6.129S-Atoh1tm6.1Hzo/J 005970 B6.129S7-Atoh1tm2Hzo/J 008681 B6.129S7-Atoh1tm3Hzo/J 011104 B6.Cg-Tg(Atoh1-cre)1Bfri/J View Strains carrying other alleles of Atoh1 (6 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
- Potential model based on transgenic expression of an ortholog of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested. Estrogen Receptor 1; ESR1 (ESR1)
Migraine with or without Aura, Susceptibility to, 1 (ESR1)
Myocardial Infarction, Susceptibility to (ESR1)
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Atoh1-cre/Esr1*)14Fsh/0
involves: FVB/N * Swiss Webster
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:105186)
This mouse can be used to support research in many areas including:
cre relatedNeurobiology Research
Cerebellar Defects
Cre-lox System
Cre Recombinase expression in neural tissue
Research Tools
Cre-lox System
Cre Recombinase Expression
Cre Recombinase Expression: Inducible
Developmental Biology Research
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Tissue/Cell Markers: neurons
Neurobiology Research
cell marker
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Atoh1-cre/Esr1*)14Fsh | ||
|---|---|---|---|
| Allele Name | transgene insertion 14, Gordon Fishell | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Atoh1-cre; Math1-CreERT2; Math1-cre; Tg(Atoh1-cre/ESR1)1Fsh; Tg(HBB-cre)1Fsh; | ||
| Mutation Made By | Rob Machold, The Skirball Institute, NYU Sch. of Med. | ||
| Strain of Origin | FVB/N | ||
| Site of Expression | tamoxifen-inducible cre activity is observed in neural progenitors of the cerebellar rhombic lip, dorsal hindbrain and spinal cord, as well as in inner-ear primordia (with a limited amount of ectopic expression in the primordium of the hippocampus but not the cortex) | ||
| Expressed Gene | ESR1, estrogen receptor 1, human | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Atoh1, atonal homolog 1 (Drosophila), mouse, laboratory | ||
| Driver Note | Atoh1 | ||
| Inducible Note | induced by tamoxifen | ||
| Molecular Note | The transgene was designed with a 1.4 kb mouse Math1 (Atoh1) 3' enhancer (and a beta-globin minimal promoter) sequence upstream of a CreERT2 fusion gene (Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain). Tamoxifen-inducible cre activity is observed in neural progenitors of the cerebellar rhombic lip, dorsal hindbrain and spinal cord, as well as in inner-ear primordia (with a limited amount of ectopic expression in the primordium of the hippocampus but not the cortex). [MGI Ref ID J:105186] | ||
| Gene Symbol and Name | Tg(Atoh1-cre/Esr1*)14Fsh, transgene insertion 14, Gordon Fishell | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Math1-CreERT2; Tg(Atoh1-cre/ESR1)14Fsh; Tg(Atoh1-cre/ESR1)1Fsh; Tg(Atoh1-creESR1)14Fsh; Tg(HBB-cre)1Fsh; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Machold R; Fishell G. 2005. Math1 is expressed in temporally discrete pools of cerebellar rhombic-lip neural progenitors. Neuron 48(1):17-24. [PubMed: 16202705] [MGI Ref ID J:105186]
Additional References
Tg(Atoh1-cre/Esr1*)14Fsh relatedAhmed M; Wong EY; Sun J; Xu J; Wang F; Xu PX. 2012. Eya1-six1 interaction is sufficient to induce hair cell fate in the cochlea by activating atoh1 expression in cooperation with sox2. Dev Cell 22(2):377-90. [PubMed: 22340499] [MGI Ref ID J:181471]
Baubet V; Xiang C; Molczan A; Roccograndi L; Melamed S; Dahmane N. 2012. Rp58 is essential for the growth and patterning of the cerebellum and for glutamatergic and GABAergic neuron development. Development 139(11):1903-9. [PubMed: 22513377] [MGI Ref ID J:184000]
Genetic Resource Sciences at The Jackson Laboratory. 2012. Expression/Specificity Patterns of Cre Alleles, 2012 MGI Direct Data Submission :. [MGI Ref ID J:184579]
Heine VM; Priller M; Ling J; Rowitch DH; Schuller U. 2010. Dexamethasone destabilizes Nmyc to inhibit the growth of hedgehog-associated medulloblastoma. Cancer Res 70(13):5220-5. [PubMed: 20530674] [MGI Ref ID J:161601]
Heine VM; Rowitch DH. 2009. Hedgehog signaling has a protective effect in glucocorticoid-induced mouse neonatal brain injury through an 11betaHSD2-dependent mechanism. J Clin Invest 119(2):267-77. [PubMed: 19164857] [MGI Ref ID J:146149]
Huang X; Dubuc AM; Hashizume R; Berg J; He Y; Wang J; Chiang C; Cooper MK; Northcott PA; Taylor MD; Barnes MJ; Tihan T; Chen J; Hackett CS; Weiss WA; James CD; Rowitch DH; Shuman MA; Jan YN; Jan LY. 2012. Voltage-gated potassium channel EAG2 controls mitotic entry and tumor growth in medulloblastoma via regulating cell volume dynamics. Genes Dev 26(16):1780-96. [PubMed: 22855790] [MGI Ref ID J:186629]
Huse JT; Holland EC. 2009. Genetically engineered mouse models of brain cancer and the promise of preclinical testing. Brain Pathol 19(1):132-43. [PubMed: 19076778] [MGI Ref ID J:173443]
Hyman JM; Firestone AJ; Heine VM; Zhao Y; Ocasio CA; Han K; Sun M; Rack PG; Sinha S; Wu JJ; Solow-Cordero DE; Jiang J; Rowitch DH; Chen JK. 2009. Small-molecule inhibitors reveal multiple strategies for Hedgehog pathway blockade. Proc Natl Acad Sci U S A 106(33):14132-7. [PubMed: 19666565] [MGI Ref ID J:151950]
Kessler JD; Hasegawa H; Brun SN; Emmenegger BA; Yang ZJ; Dutton JW; Wang F; Wechsler-Reya RJ. 2009. N-myc alters the fate of preneoplastic cells in a mouse model of medulloblastoma. Genes Dev 23(2):157-70. [PubMed: 19171780] [MGI Ref ID J:143859]
Lee MJ; Hatton BA; Villavicencio EH; Khanna PC; Friedman SD; Ditzler S; Pullar B; Robison K; White KF; Tunkey C; Leblanc M; Randolph-Habecker J; Knoblaugh SE; Hansen S; Richards A; Wainwright BJ; McGovern K; Olson JM. 2012. Hedgehog pathway inhibitor saridegib (IPI-926) increases lifespan in a mouse medulloblastoma model. Proc Natl Acad Sci U S A 109(20):7859-64. [PubMed: 22550175] [MGI Ref ID J:184789]
Lorenz A; Deutschmann M; Ahlfeld J; Prix C; Koch A; Smits R; Fodde R; Kretzschmar HA; Schuller U. 2011. Severe alterations of cerebellar cortical development after constitutive activation of wnt signaling in granule neuron precursors. Mol Cell Biol 31(16):3326-38. [PubMed: 21690300] [MGI Ref ID J:175083]
Ma S; Kwon HJ; Huang Z. 2012. Ric-8a, a guanine nucleotide exchange factor for heterotrimeric G proteins, regulates bergmann glia-basement membrane adhesion during cerebellar foliation. J Neurosci 32(43):14979-93. [PubMed: 23100420] [MGI Ref ID J:191222]
Maricich SM; Xia A; Mathes EL; Wang VY; Oghalai JS; Fritzsch B; Zoghbi HY. 2009. Atoh1-lineal neurons are required for hearing and for the survival of neurons in the spiral ganglion and brainstem accessory auditory nuclei. J Neurosci 29(36):11123-33. [PubMed: 19741118] [MGI Ref ID J:152678]
Poschl J; Lorenz A; Hartmann W; von Bueren AO; Kool M; Li S; Peraud A; Tonn JC; Herms J; Xiang M; Rutkowski S; Kretzschmar HA; Schuller U. 2011. Expression of BARHL1 in medulloblastoma is associated with prolonged survival in mice and humans. Oncogene 30(47):4721-30. [PubMed: 21602885] [MGI Ref ID J:178575]
Sakai N; Insolera R; Sillitoe RV; Shi SH; Kaprielian Z. 2012. Axon sorting within the spinal cord marginal zone via Robo-mediated inhibition of N-cadherin controls spinocerebellar tract formation. J Neurosci 32(44):15377-87. [PubMed: 23115176] [MGI Ref ID J:192292]
Schuller U; Heine VM; Mao J; Kho AT; Dillon AK; Han YG; Huillard E; Sun T; Ligon AH; Qian Y; Ma Q; Alvarez-Buylla A; McMahon AP; Rowitch DH; Ligon KL. 2008. Acquisition of granule neuron precursor identity is a critical determinant of progenitor cell competence to form Shh-induced medulloblastoma. Cancer Cell 14(2):123-34. [PubMed: 18691547] [MGI Ref ID J:139574]
Schuller U; Zhao Q; Godinho SA; Heine VM; Medema RH; Pellman D; Rowitch DH. 2007. Forkhead transcription factor FoxM1 regulates mitotic entry and prevents spindle defects in cerebellar granule neuron precursors. Mol Cell Biol 27(23):8259-70. [PubMed: 17893320] [MGI Ref ID J:129035]
Sudarov A; Joyner AL. 2007. Cerebellum morphogenesis: the foliation pattern is orchestrated by multi-cellular anchoring centers. Neural Dev 2:26. [PubMed: 18053187] [MGI Ref ID J:160871]
Yang ZJ; Ellis T; Markant SL; Read TA; Kessler JD; Bourboulas M; Schuller U; Machold R; Fishell G; Rowitch DH; Wainwright BJ; Wechsler-Reya RJ. 2008. Medulloblastoma can be initiated by deletion of Patched in lineage-restricted progenitors or stem cells. Cancer Cell 14(2):135-45. [PubMed: 18691548] [MGI Ref ID J:139573]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, transgenic mice can be bred to wildtype siblings or bred together, although a homozygous phenotype is not yet characterized (Aug-2007). Mating System Noncarrier x Hemizygote (Female x Male) 10-APR-08 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(Atoh1-cre/Esr1*)14Fsh
Price per Pair (US dollars $) Pair Genotype $302.00 Hemizygous for Tg(Atoh1-cre/Esr1*)14Fsh x Noncarrier $302.00 Noncarrier x Hemizygous for Tg(Atoh1-cre/Esr1*)14Fsh Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(Atoh1-cre/Esr1*)14Fsh
Price per Pair (US dollars $) Pair Genotype $392.60 Hemizygous for Tg(Atoh1-cre/Esr1*)14Fsh x Noncarrier $392.60 Noncarrier x Hemizygous for Tg(Atoh1-cre/Esr1*)14Fsh Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
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|
|
Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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