Strain Name:

BKa.Cg-Sox17tm2Sjm Ptprcb Thy1a/J

Stock Number:

007686

Order this mouse

Availability:

Cryopreserved - Ready for recovery

When crossed with a Cre recombinase-expressing strain, this strain carrying a floxed allele is useful in eliminating tissue-specific expression of the gene. This mutant mouse strain may be useful in studies of fetal and neonatal hematopoietic stem cells and hematopoiesis.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
 
Donating Investigator Sean J Morrison,   UT Southwestern

Description
These mice possess loxP sites on either side of the exon 3-5 region of the targeted gene. Mice homozygous for this allele are viable and fertile and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene.

When the floxed allele is excised by crosses with a Tie2 (endothelial-specific receptor tyrosine kinase)-Cre mouse (Stock No. 004128) and the Sox17 targeted mutant EGFP reporter strain (Stock No. 007687), mutants are embryonic lethal around E13.5. Mutant embryos have severe hematopoietic failure and lack definitive hematopoietic stem cells.

When the floxed allele is excised by Mx1 (myxovirus (influenza virus) resistance 1)-Cre (Stock No. 003556) and the Sox17 targeted mutant EGFP reporter strain (Stock No. 007687), neonatal mutant mice die after one week, showing hematopoietic defects. This mutant mouse strain may be useful in studies of fetal and neonatal hematopoietic stem cells and hematopoiesis.

Development
A targeting vector was designed to flank the exon 3-5 region with loxP sites and place an FRT-flanked neomycin resistance cassette in intron 5. Exons 4 and 5 contain the coding sequence. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/Ka CD45.2:Thy1.1 (Ptprcb Thy1a) mice. A cross to a C57BL/6-background Actb-FLP strain was then carried out to remove the FRT-flanked neomycin cassette. The line was then backcrossed to the C57BL/Ka CD45.2:Thy1.1 line for 8 generations by the donating laboratory.

Related Strains

View Strains carrying   Thy1a     (19 strains)

View Strains carrying other alleles of Ptprc     (18 strains)

Strains carrying other alleles of Sox17
007687   BKa.Cg-Sox17tm1Sjm Ptprcb Thy1a/J
View Strains carrying other alleles of Sox17     (1 strain)

Strains carrying other alleles of Thy1
017798   B6.Cg-Mapttm1Hnd Tg(Thy1-MAPT*)3610Gds/Mmjax
009126   B6.Cg-Nos2tm1Lau Tg(Thy1-APPSwDutIowa)BWevn/Mmjax
008730   B6.Cg-Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas/Mmjax
007901   B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J
007911   B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J
007921   B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J
003710   B6.Cg-Tg(Thy1-CFP)23Jrs/J
014131   B6.Cg-Tg(Thy1-CFP)IJrs/GfngJ
007940   B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J
007967   B6.Cg-Tg(Thy1-CFP/COX8A)S2Lich/J
012597   B6.Cg-Tg(Thy1-COL25A1)861Yfu/J
007612   B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J
007615   B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J
013161   B6.Cg-Tg(Thy1-Clomeleon)1Gjau/J
007919   B6.Cg-Tg(Thy1-EGFP)OJrs/GfngJ
005630   B6.Cg-Tg(Thy1-EYFP)15Jrs/J
009611   B6.Cg-Tg(Thy1-Nlgn1)6Hnes/J
009612   B6.Cg-Tg(Thy1-Nlgn2)6Hnes/J
021069   B6.Cg-Tg(Thy1-PA-GFP)5Rmpl/J
021070   B6.Cg-Tg(Thy1-PA-GFP)6Rmpl/J
003709   B6.Cg-Tg(Thy1-YFP)16Jrs/J
003782   B6.Cg-Tg(Thy1-YFP)HJrs/J
005627   B6.Cg-Tg(Thy1-YFP/Syp)10Jrs/J
007606   B6.Cg-Tg(Thy1-cre/ERT2,-EYFP)AGfng/J
004807   B6;129-Psen1tm1Mpm Tg(APPSwe,tauP301L)1Lfa/Mmjax
007910   B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J
011070   B6;CBA-Tg(Thy1-EGFP)SJrs/NdivJ
017892   B6;CBA-Tg(Thy1-GCaMP2.2c)8Gfng/J
017893   B6;CBA-Tg(Thy1-GCaMP3)6Gfng/J
014130   B6;CBA-Tg(Thy1-YFP)GJrs/GfngJ
014651   B6;CBA-Tg(Thy1-spH)21Vnmu/J
015814   B6;CBA-Tg(Thy1-spH)64Vnmu/FrkJ
012341   B6;SJL-Tg(Thy1-COP3/EYFP)1Gfng/J
012344   B6;SJL-Tg(Thy1-COP3/EYFP)4Gfng/J
012348   B6;SJL-Tg(Thy1-COP3/EYFP)8Gfng/J
012350   B6;SJL-Tg(Thy1-COP4*H134R/EYFP)20Gfng/J
008004   B6;SJL-Tg(Thy1-ECFP/VAMP2)1Sud/J
012836   B6;SJL-Tg(Thy1-TARDBP)4Singh/J
007610   B6;SJL-Tg(Thy1-cre/ERT2,-EYFP)VGfng/J
012332   B6;SJL-Tg(Thy1-hop/EYFP)2Gfng/J
012334   B6;SJL-Tg(Thy1-hop/EYFP)4Gfng/J
006554   B6SJL-Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas/Mmjax
025401   B6SJL-Tg(Thy1-COX8A/Dendra)57Gmnf/J
017590   B6SJL-Tg(Thy1-DCTN1*G59S)M2Pcw/J
007880   B6SJL-Tg(Thy1-Stx1a/EYFP)1Sud/J
007856   B6SJL-Tg(Thy1-Syt1/ECFP)1Sud/J
017589   B6SJL-Tg(Thy1-TARDBP*G298S)S97Pcw/J
024703   C3A.Cg-Pde6b+Tg(Thy1-CFP)23Jrs/SjJ
007027   C57BL/6-Tg(Thy1-APPSwDutIowa)BWevn/Mmjax
010800   C57BL/6-Tg(Thy1-PTGS2)300Kand/J
010703   C57BL/6-Tg(Thy1-PTGS2)303Kand/J
012769   C57BL/6-Tg(Thy1-Sncg)HvP36Putt/J
024339   C57BL/6J-Tg(Thy1-GCaMP6f)GP5.11Dkim/J
025393   C57BL/6J-Tg(Thy1-GCaMP6f)GP5.17Dkim/J
024276   C57BL/6J-Tg(Thy1-GCaMP6f)GP5.5Dkim/J
025776   C57BL/6J-Tg(Thy1-GCaMP6s)GP4.12Dkim/J
024275   C57BL/6J-Tg(Thy1-GCaMP6s)GP4.3Dkim/J
025533   C57BL/6N-Sncatm1Mjff Tg(Thy1-SNCA)15Mjff/J
016936   C57BL/6N-Tg(Thy1-SNCA)12Mjff/J
017682   C57BL/6N-Tg(Thy1-SNCA)15Mjff/J
024704   D2.Cg-Gpnmb+Tg(Thy1-CFP)23Jrs/SjJ
025018   D2.Cg-Gpnmb+Tg(Thy1-YFP)HJrs/SjJ
018671   D2.Cg-Tg(Thy1-CFP)23Jrs/SjJ
024705   D2.Cg-Tg(Thy1-YFP)HJrs/SjJ
025019   D2.Cg-Tg(Thy1-YFP/Syp)10Jrs/SjJ
008230   FVB(Cg)-Tg(Thy1-SOD1*G93A)T3Hgrd/J
006143   FVB/N-Tg(Thy1-cre)1Vln/J
021226   STOCK Tg(Thy1-Brainbow3.1)18Jrs/J
021225   STOCK Tg(Thy1-Brainbow3.1)3Jrs/J
021227   STOCK Tg(Thy1-Brainbow3.2)7Jrs/J
013162   STOCK Tg(Thy1-Clomeleon)12Gjau/J
013163   STOCK Tg(Thy1-Clomeleon)13Gjau/J
007788   STOCK Tg(Thy1-EGFP)MJrs/J
012708   STOCK Tg(Thy1-cre/ERT2,-EYFP)HGfng/PyngJ
View Strains carrying other alleles of Thy1     (74 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Vesicoureteral Reflux 3; VUR3   (SOX17)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Sox17tm1Sjm/Sox17tm2Sjm Tg(Mx1-cre)1Cgn/0

        involves: 129/Sv * C3H * C57BL/6 * C57BL/Ka * CBA   (conditional)
  • mortality/aging
  • complete postnatal lethality
    • death of all mice by 14 days after birth   (MGI Ref ID J:123050)
  • hematopoietic system phenotype
  • decreased bone marrow cell number
    • when sacrificed 4-5 days after end of poly(I:C) treatment, reduced bone marrow cellularity is observed   (MGI Ref ID J:123050)
  • decreased hematopoietic stem cell number
    • HSCs are reduced in bone marrow and spleen   (MGI Ref ID J:123050)
  • decreased leukocyte cell number
    • numbers are significantly reduce compared to controls following cre induction   (MGI Ref ID J:123050)
  • decreased platelet cell number
    • numbers are significantly reduce compared to controls following cre induction   (MGI Ref ID J:123050)
  • spleen hypoplasia
    • when sacrificed 4-5 days after end of poly(I:C) treatment, reduced spleen cellularity is observed   (MGI Ref ID J:123050)
  • thymus hypoplasia
    • mice display severe reduction in thymus cellularity upon examination at 4-5 days after end of poly(I:C) treatment   (MGI Ref ID J:123050)
  • immune system phenotype
  • decreased leukocyte cell number
    • numbers are significantly reduce compared to controls following cre induction   (MGI Ref ID J:123050)
  • spleen hypoplasia
    • when sacrificed 4-5 days after end of poly(I:C) treatment, reduced spleen cellularity is observed   (MGI Ref ID J:123050)
  • thymus hypoplasia
    • mice display severe reduction in thymus cellularity upon examination at 4-5 days after end of poly(I:C) treatment   (MGI Ref ID J:123050)
  • endocrine/exocrine gland phenotype
  • thymus hypoplasia
    • mice display severe reduction in thymus cellularity upon examination at 4-5 days after end of poly(I:C) treatment   (MGI Ref ID J:123050)

Sox17tm1Sjm/Sox17tm2Sjm Tg(Tek-cre)12Flv/0

        involves: 129S1/Sv * 129X1/SvJ * C3H * C57BL/6 * C57BL/Ka   (conditional)
  • mortality/aging
  • complete embryonic lethality during organogenesis
    • expected numbers of homozygotes are found at E12.5, but complete lethality is observed by E13.5   (MGI Ref ID J:123050)
  • embryogenesis phenotype
  • abnormal embryonic hematopoiesis
    • no hematopoiesis is visible in the yolk sac or embryo at E12.5   (MGI Ref ID J:123050)
  • embryonic growth retardation
    • at E12.5, mutants are growth retarded   (MGI Ref ID J:123050)
  • growth/size/body phenotype
  • embryonic growth retardation
    • at E12.5, mutants are growth retarded   (MGI Ref ID J:123050)
  • hematopoietic system phenotype
  • abnormal embryonic hematopoiesis
    • no hematopoiesis is visible in the yolk sac or embryo at E12.5   (MGI Ref ID J:123050)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Hematological Research
Hematopoietic Defects

Research Tools
Cre-lox System
      loxP-flanked Sequences

Thy1a related

Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Research Tools
Genetics Research
      Tissue/Cell Markers
      Tissue/Cell Markers: T cell specific surface marker
Immunology, Inflammation and Autoimmunity Research
      T cell specific surface marker

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Sox17tm2Sjm
Allele Name targeted mutation 2, Sean J Morrison
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) Sox17cKO; Sox17fl;
Mutation Made By Sean Morrison,   UT Southwestern
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line NameR1
ES Cell Line Strain(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Sox17, SRY (sex determining region Y)-box 17
Chromosome 1
Gene Common Name(s) VUR3;
Molecular Note LoxP sites were inserted on either side of the exon 3-5 region of the Sox17 gene. Mice homozygous for this allele are viable and fertile and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. [MGI Ref ID J:123050]
 
Allele Symbol Thy1a
Allele Name a variant
Allele Type Not Applicable
Common Name(s) Thy-1.1; Thy1.1; Thy1a; theta-AKR; thetaAKR;
Site of ExpressionThe Thy1 locus determines a surface antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells.
Gene Symbol and Name Thy1, thymus cell antigen 1, theta
Chromosome 9
Gene Common Name(s) CD7; CD90; T25; Thy 1.2; Thy-1; Thy-1.2; Thy1.1; Thy1.2; theta;
General Note

The Thy1 locus determines a surface antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells. The allele Thy1a determines an antigenic specificity, Thy-1.1, found in the AKR and RF strains; the allele Thy1b determines an antigenic specificity, Thy-1.2, found in the C3HeB/Fe and many other strains (J:5243, J:5012, J:4469). The Thy1 antigen is probably present on all T lymphocytes and absent from all B lymphocytes, and it thus serves as a valuable T-cell marker (J:5243). It is very widely used in experiments designed to determine the distribution and function of T-cells. Thy1 specifies a T-cell surface glycoprotein, T25, with a molecular weight of 25 kDa (J:5707). The protein appears to be anchored in the cell membrane by a lipid that is either phosphotidylinositol or closely related to it (J:12016). Thy1 may function in the cell membrane as a signal transduction molecule (J:8333). The Thy1 locus, or possibly a gene closely linked to it, controls quantitative expression of a protein that isthe same size as Thy1 and is expressed on thymus and brain but not on lymph node and spleen cells (J:7900).

Molecular Note The allele Thy1a determines an antigenic specificity, Thy-1.1, found in the AKR and RF strains.
 
Gene Symbol and Name Ptprc, protein tyrosine phosphatase, receptor type, C
Chromosome 1
Gene Common Name(s) B220; CD45; CD45 antigen; CD45R; Cd45; GP180; L-CA; LCA; LY5; Ly-5; Lyt-4; RT7; T-lymphocyte antigen 4; T200; lymphocyte antigen 5;

Genotyping

Genotyping Information

Genotyping Protocols

Sox17tm2Sjm, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Kim I; Saunders TL; Morrison SJ. 2007. Sox17 dependence distingushes the transcriptional regulation of fetal from adult hematopoietic stem cells Cell 130(3):470-83. [PubMed: 17655922]  [MGI Ref ID J:123050]

Additional References

Sox17tm2Sjm related

Artus J; Piliszek A; Hadjantonakis AK. 2011. The primitive endoderm lineage of the mouse blastocyst: sequential transcription factor activation and regulation of differentiation by Sox17. Dev Biol 350(2):393-404. [PubMed: 21146513]  [MGI Ref ID J:170416]

Viotti M; Niu L; Shi SH; Hadjantonakis AK. 2012. Role of the gut endoderm in relaying left-right patterning in mice. PLoS Biol 10(3):e1001276. [PubMed: 22412348]  [MGI Ref ID J:184721]

Yang H; Lee S; Lee S; Kim K; Yang Y; Kim JH; Adams RH; Wells JM; Morrison SJ; Koh GY; Kim I. 2013. Sox17 promotes tumor angiogenesis and destabilizes tumor vessels in mice. J Clin Invest 123(1):418-31. [PubMed: 23241958]  [MGI Ref ID J:194296]

Thy1a related

Azzi J; Skartsis N; Mounayar M; Magee CN; Batal I; Ting C; Moore R; Riella LV; Ohori S; Abdoli R; Smith B; Fiorina P; Heathcote D; Bakhos T; Ashton-Rickardt PG; Abdi R. 2013. Serine protease inhibitor 6 plays a critical role in protecting murine granzyme B-producing regulatory T cells. J Immunol 191(5):2319-27. [PubMed: 23913965]  [MGI Ref ID J:205809]

Beck PL; Li Y; Wong J; Chen CW; Keenan CM; Sharkey KA; McCafferty DM. 2007. Inducible nitric oxide synthase from bone marrow-derived cells plays a critical role in regulating colonic inflammation. Gastroenterology 132(5):1778-90. [PubMed: 17449036]  [MGI Ref ID J:128325]

Brinkman CC; Rouhani SJ; Srinivasan N; Engelhard VH. 2013. Peripheral tissue homing receptors enable T cell entry into lymph nodes and affect the anatomical distribution of memory cells. J Immunol 191(5):2412-25. [PubMed: 23926324]  [MGI Ref ID J:205788]

Chen TT; Li L; Chung DH; Allen CD; Torti SV; Torti FM; Cyster JG; Chen CY; Brodsky FM; Niemi EC; Nakamura MC; Seaman WE; Daws MR. 2005. TIM-2 is expressed on B cells and in liver and kidney and is a receptor for H-ferritin endocytosis. J Exp Med 202(7):955-65. [PubMed: 16203866]  [MGI Ref ID J:107466]

Cuda CM; Wan S; Sobel ES; Croker BP; Morel L. 2007. Murine lupus susceptibility locus Sle1a controls regulatory T cell number and function through multiple mechanisms. J Immunol 179(11):7439-47. [PubMed: 18025188]  [MGI Ref ID J:154964]

D'Eustachio P; Owens GC; Edelman GM; Cunningham BA. 1985. Chromosomal location of the gene encoding the neural cell adhesion molecule (N-CAM) in the mouse. Proc Natl Acad Sci U S A 82(22):7631-5. [PubMed: 3865183]  [MGI Ref ID J:8111]

Dewals B; Hoving JC; Horsnell WG; Brombacher F. 2010. Control of Schistosoma mansoni egg-induced inflammation by IL-4-responsive CD4(+)CD25(-)CD103(+)Foxp3(-) cells is IL-10-dependent. Eur J Immunol 40(10):2837-47. [PubMed: 20821727]  [MGI Ref ID J:165816]

Divangahi M; Desjardins D; Nunes-Alves C; Remold HG; Behar SM. 2010. Eicosanoid pathways regulate adaptive immunity to Mycobacterium tuberculosis. Nat Immunol 11(8):751-8. [PubMed: 20622882]  [MGI Ref ID J:162390]

Dolfi DV; Duttagupta PA; Boesteanu AC; Mueller YM; Oliai CH; Borowski AB; Katsikis PD. 2011. Dendritic cells and CD28 costimulation are required to sustain virus-specific CD8+ T cell responses during the effector phase in vivo. J Immunol 186(8):4599-608. [PubMed: 21389258]  [MGI Ref ID J:172460]

Ertelt JM; Buyukbasaran EZ; Jiang TT; Rowe JH; Xin L; Way SS. 2013. B7-1/B7-2 blockade overrides the activation of protective CD8 T cells stimulated in the absence of Foxp3+ regulatory T cells. J Leukoc Biol 94(2):367-76. [PubMed: 23744647]  [MGI Ref ID J:204423]

Fife BT; Griffin MD; Abbas AK; Locksley RM; Bluestone JA. 2006. Inhibition of T cell activation and autoimmune diabetes using a B cell surface-linked CTLA-4 agonist. J Clin Invest 116(8):2252-61. [PubMed: 16886063]  [MGI Ref ID J:113109]

Green MC; Sweet HO. 1975. [Hx - Hm - W.] Mouse News Lett 52:38.  [MGI Ref ID J:13675]

Huang W; Huang F; Kannan AK; Hu J; August A. 2014. ITK tunes IL-4-induced development of innate memory CD8+ T cells in a gammadelta T and invariant NKT cell-independent manner. J Leukoc Biol 96(1):55-63. [PubMed: 24620029]  [MGI Ref ID J:212002]

Inlay MA; Bhattacharya D; Sahoo D; Serwold T; Seita J; Karsunky H; Plevritis SK; Dill DL; Weissman IL. 2009. Ly6d marks the earliest stage of B-cell specification and identifies the branchpoint between B-cell and T-cell development. Genes Dev 23(20):2376-81. [PubMed: 19833765]  [MGI Ref ID J:154864]

Kelly LM; Pereira JP; Yi T; Xu Y; Cyster JG. 2011. EBI2 guides serial movements of activated B cells and ligand activity is detectable in lymphoid and nonlymphoid tissues. J Immunol 187(6):3026-32. [PubMed: 21844396]  [MGI Ref ID J:179238]

Klebanoff CA; Spencer SP; Torabi-Parizi P; Grainger JR; Roychoudhuri R; Ji Y; Sukumar M; Muranski P; Scott CD; Hall JA; Ferreyra GA; Leonardi AJ; Borman ZA; Wang J; Palmer DC; Wilhelm C; Cai R; Sun J; Napoli JL; Danner RL; Gattinoni L; Belkaid Y; RestifoNP. 2013. Retinoic acid controls the homeostasis of pre-cDC-derived splenic and intestinal dendritic cells. J Exp Med 210(10):1961-76. [PubMed: 23999499]  [MGI Ref ID J:203429]

Krieg C; Letourneau S; Pantaleo G; Boyman O. 2010. Improved IL-2 immunotherapy by selective stimulation of IL-2 receptors on lymphocytes and endothelial cells. Proc Natl Acad Sci U S A 107(26):11906-11. [PubMed: 20547866]  [MGI Ref ID J:161365]

Lee SY; Goverman JM. 2013. The influence of T cell Ig mucin-3 signaling on central nervous system autoimmune disease is determined by the effector function of the pathogenic T cells. J Immunol 190(10):4991-9. [PubMed: 23562810]  [MGI Ref ID J:202569]

Leiter EH. 1998. NOD Mice and Related Strains: Origins, Husbandry and Biology Introduction. In: NOD Mice and Related Strains: Research Applications in Diabetes, AIDS, Cancer, and Other Diseases. RG Landes, Austin.  [MGI Ref ID J:110093]

Mitchell JS; Burbach BJ; Srivastava R; Fife BT; Shimizu Y. 2013. Multistage T cell-dendritic cell interactions control optimal CD4 T cell activation through the ADAP-SKAP55-signaling module. J Immunol 191(5):2372-83. [PubMed: 23918975]  [MGI Ref ID J:205802]

Pauken KE; Jenkins MK; Azuma M; Fife BT. 2013. PD-1, but not PD-L1, expressed by islet-reactive CD4+ T cells suppresses infiltration of the pancreas during type 1 diabetes. Diabetes 62(8):2859-69. [PubMed: 23545706]  [MGI Ref ID J:208973]

REIF AE; ALLEN JM. 1964. THE AKR THYMIC ANTIGEN AND ITS DISTRIBUTION IN LEUKEMIAS AND NERVOUS TISSUES. J Exp Med 120:413-33. [PubMed: 14207060]  [MGI Ref ID J:24839]

Rabenstein H; Behrendt AC; Ellwart JW; Naumann R; Horsch M; Beckers J; Obst R. 2014. Differential kinetics of antigen dependency of CD4+ and CD8+ T cells. J Immunol 192(8):3507-17. [PubMed: 24639353]  [MGI Ref ID J:210002]

Ranheim EA; Tarbell KV; Krogsgaard M; Mallet-Designe V; Teyton L; McDevitt HO; Weissman IL. 2004. Selection of aberrant class II restricted CD8+ T cells in NOD mice expressing a glutamic acid decarboxylase (GAD)65-specific T cell receptor transgene. Autoimmunity 37(8):555-67. [PubMed: 15763918]  [MGI Ref ID J:128250]

Read S; Hogan TV; Zwar TD; Gleeson PA; Van Driel IR. 2007. Prevention of autoimmune gastritis in mice requires extra-thymic T-cell deletion and suppression by regulatory T cells. Gastroenterology 133(2):547-58. [PubMed: 17603058]  [MGI Ref ID J:128303]

Reif AE; Allen JM. 1966. Mouse thymic iso-antigens. Nature 209(22):521-3. [PubMed: 5919593]  [MGI Ref ID J:5012]

Rodeghero R; Cao Y; Olalekan SA; Iwakua Y; Glant TT; Finnegan A. 2013. Location of CD4+ T cell priming regulates the differentiation of Th1 and Th17 cells and their contribution to arthritis. J Immunol 190(11):5423-35. [PubMed: 23630349]  [MGI Ref ID J:204778]

Sercan O; Stoycheva D; Hammerling GJ; Arnold B; Schuler T. 2010. IFN-gamma receptor signaling regulates memory CD8+ T cell differentiation. J Immunol 184(6):2855-62. [PubMed: 20164422]  [MGI Ref ID J:160112]

Uhl M; Kepp O; Jusforgues-Saklani H; Vicencio JM; Kroemer G; Albert ML. 2009. Autophagy within the antigen donor cell facilitates efficient antigen cross-priming of virus-specific CD8+ T cells. Cell Death Differ 16(7):991-1005. [PubMed: 19229247]  [MGI Ref ID J:164191]

Voehringer D; Liang HE; Locksley RM. 2008. Homeostasis and effector function of lymphopenia-induced 'memory-like' T cells in constitutively T cell-depleted mice. J Immunol 180(7):4742-53. [PubMed: 18354198]  [MGI Ref ID J:133382]

Wang L; Jameson SC; Hogquist KA. 2009. Epidermal Langerhans cells are not required for UV-induced immunosuppression. J Immunol 183(9):5548-53. [PubMed: 19843938]  [MGI Ref ID J:156799]

Wuthrich M; Ersland K; Pick-Jacobs JC; Gern BH; Frye CA; Sullivan TD; Brennan MB; Filutowicz HI; O'Brien K; Korthauer KD; Schultz-Cherry S; Klein BS. 2012. Limited model antigen expression by transgenic fungi induces disparate fates during differentiation of adoptively transferred T cell receptor transgenic CD4+ T cells: robust activation and proliferation with weak effector function during recall. Infect Immun 80(2):787-97. [PubMed: 22124658]  [MGI Ref ID J:180817]

Wuthrich M; Warner T; Klein BS. 2005. IL-12 is required for induction but not maintenance of protective, memory responses to Blastomyces dermatitidis: implications for vaccine development in immune-deficient hosts. J Immunol 175(8):5288-97. [PubMed: 16210634]  [MGI Ref ID J:119110]

Xiao Z; Mescher MF; Jameson SC. 2007. Detuning CD8 T cells: down-regulation of CD8 expression, tetramer binding, and response during CTL activation. J Exp Med 204(11):2667-77. [PubMed: 17954566]  [MGI Ref ID J:126126]

Ysebrant de Lendonck L; Tonon S; Nguyen M; Vandevenne P; Welsby I; Martinet V; Molle C; Charbonnier LM; Leo O; Goriely S. 2013. Interferon regulatory factor 3 controls interleukin-17 expression in CD8 T lymphocytes. Proc Natl Acad Sci U S A 110(34):E3189-97. [PubMed: 23918362]  [MGI Ref ID J:200677]

Yu Y; Cho HI; Wang D; Kaosaard K; Anasetti C; Celis E; Yu XZ. 2013. Adoptive Transfer of Tc1 or Tc17 Cells Elicits Antitumor Immunity against Established Melanoma through Distinct Mechanisms. J Immunol 190(4):1873-81. [PubMed: 23315072]  [MGI Ref ID J:193239]

Zaleski M; Klein J. 1974. Immune response of mice to Thy-1. 1 antigen: genetic control by alleles at the Ir-5 locus loosely linked to the H-2 complex. J Immunol 113(4):1170-7. [PubMed: 4606643]  [MGI Ref ID J:5487]

Zaleski MB. 1975. Immune response of mice to the Thy-1.1 antigen: effect of the Ir-5 alleles studies in 129/J and B10.129(6M) mice Immunogenetics 2:241-8.  [MGI Ref ID J:30773]

Zecher D; Li Q; Oberbarnscheidt MH; Demetris AJ; Shlomchik WD; Rothstein DM; Lakkis FG. 2010. NK cells delay allograft rejection in lymphopenic hosts by downregulating the homeostatic proliferation of CD8+ T cells. J Immunol 184(12):6649-57. [PubMed: 20483732]  [MGI Ref ID J:161405]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.8)