Strain Name:

BKa.Cg-Sox17tm2Sjm Ptprcb Thy1a/J

Stock Number:

007686

Availability:

Repository-Cryopreserved
When crossed with a Cre recombinase-expressing strain, this strain carrying a floxed allele is useful in eliminating tissue-specific expression of the gene. This mutant mouse strain may be useful in studies of fetal and neonatal hematopoietic stem cells and hematopoiesis.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered Mutant Mice.
Specieslaboratory mouse
 
Donating Investigator Sean Morrison,   University of Michigan

Description
These mice possess loxP sites on either side of the exon 3-5 region of the targeted gene. Mice homozygous for this allele are viable and fertile and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene.

When the floxed allele is excised by crosses with a Tie2 (endothelial-specific receptor tyrosine kinase)-Cre mouse (Stock No. 004128) and the Sox17 targeted mutant EGFP reporter strain (Stock No. 007687), mutants are embryonic lethal around E13.5. Mutant embryos have severe hematopoietic failure and lack definitive hematopoietic stem cells.

When the floxed allele is excised by Mx1 (myxovirus (influenza virus) resistance 1)-Cre (Stock No. 003556) and the Sox17 targeted mutant EGFP reporter strain (Stock No. 007687), neonatal mutant mice die after one week, showing hematopoietic defects. This mutant mouse strain may be useful in studies of fetal and neonatal hematopoietic stem cells and hematopoiesis.

Development
A targeting vector was designed to flank the exon 3-5 region with loxP sites and place an FRT-flanked neomycin resistance cassette in intron 5. Exons 4 and 5 contain the coding sequence. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/Ka CD45.2:Thy1.1 (Ptprcb Thy1a) mice. A cross to a C57BL/6-background Actb-FLP strain was then carried out to remove the FRT-flanked neomycin cassette. The line was then backcrossed to the C57BL/Ka CD45.2:Thy1.1 line for 8 generations by the donating laboratory.

Related Strains

View Strains carrying   Thy1a     (14 strains)

Additional Web Information

Congenic Nomenclature
Cre-lox Systems

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Sox17tm1Sjm/Sox17tm2Sjm Tg(Mx1-cre)1Cgn/0

        involves: 129S1/Sv * 129X1/SvJ * C3H * C57BL/6 * C57BL/Ka   (conditional)
  • lethality-postnatal
  • postnatal lethality (MGI Ref ID J:123050)
  • hematopoietic system phenotype
  • decreased bone marrow cell number (MGI Ref ID J:123050)
    • when sacrificed 4-5 days after end of poly(I:C) treatment, reduced bone marrow cellularity is observed
  • decreased hematopoietic stem cell number (MGI Ref ID J:123050)
    • HSCs are reduced in bone marrow and spleen
  • decreased leukocyte cell number (MGI Ref ID J:123050)
    • numbers are significantly reduce compared to controls following cre induction
  • decreased platelet cell number (MGI Ref ID J:123050)
    • numbers are significantly reduce compared to controls following cre induction
  • spleen hypoplasia (MGI Ref ID J:123050)
    • when sacrificed 4-5 days after end of poly(I:C) treatment, reduced spleen cellularity is observed
  • thymus hypoplasia (MGI Ref ID J:123050)
    • mice display severe reduction in thymus cellularity upon examination at 4-5 days after end of poly(I:C) treatment
  • immune system phenotype
  • decreased leukocyte cell number (MGI Ref ID J:123050)
    • numbers are significantly reduce compared to controls following cre induction
  • spleen hypoplasia (MGI Ref ID J:123050)
    • when sacrificed 4-5 days after end of poly(I:C) treatment, reduced spleen cellularity is observed
  • thymus hypoplasia (MGI Ref ID J:123050)
    • mice display severe reduction in thymus cellularity upon examination at 4-5 days after end of poly(I:C) treatment

Sox17tm1Sjm/Sox17tm2Sjm Tg(Tek-cre)12Flv/0

        involves: 129S1/Sv * 129X1/SvJ * C3H * C57BL/6 * C57BL/Ka   (conditional)
  • lethality-prenatal/perinatal
  • embryonic lethality during organogenesis (MGI Ref ID J:123050)
    • expected numbers of homozygotes are found at E12.5, but complete lethality is observed by E13.5
  • embryogenesis phenotype
  • abnormal embryonic hematopoiesis (MGI Ref ID J:123050)
    • no hematopoiesis is visible in the yolk sac or embryo at E12.5
  • embryonic growth retardation (MGI Ref ID J:123050)
    • at E12.5, mutants are growth retarded
  • growth/size phenotype
  • embryonic growth retardation (MGI Ref ID J:123050)
    • at E12.5, mutants are growth retarded
  • hematopoietic system phenotype
  • abnormal embryonic hematopoiesis (MGI Ref ID J:123050)
    • no hematopoiesis is visible in the yolk sac or embryo at E12.5
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Hematological Research
Hematopoietic Defects

Research Tools
Cre-lox System (loxP-flanked Sequences)

Thy1a related

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Research Tools
Genetics Research (Tissue/Cell Markers: T cell specific surface marker)
Immunology and Inflammation Research (T cell specific surface marker)

Genes & Alleles

Gene & Allele Information

Allele Symbol Sox17tm2Sjm
Allele Name targeted mutation 2, Sean J Morrison
Allele Type Targeted (Floxed/Frt)
Common Name(s) Sox17fl;
Mutation Made By Sean Morrison,   University of Michigan
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl+
ES Cell Line NameR1
ES Cell Line Strain(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Sox17, SRY-box containing gene 17
Chromosome 1
Gene Common Name(s) FLJ22252;
Molecular Note LoxP sites were inserted on either side of the exon 3-5 region of the Sox17 gene. Mice homozygous for this allele are viable and fertile and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. [MGI Ref ID J:123050]
 
Allele Symbol Thy1a
Allele Name a variant
Allele Type Not Applicable
Common Name(s) Thy-1.1; Thy1.1; Thy1a;
Gene Symbol and Name Thy1, thymus cell antigen 1, theta
Chromosome 9
Gene Common Name(s) CD7; CD90; FLJ33325; T25; Thy 1.2; Thy-1; Thy-1.2; Thy1.1; Thy1.2;
General Note

The Thy1 locus determines an antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells. The allele Thy1a determines an antigenic specificity, Thy-1.1, found in the AKR and RF strains; the allele Thy1b determines an antigenic specificity, Thy-1.2, found in the C3HeB/Fe and many other strains (J:5243, J:5012, J:4469). The Thy1 antigen is probably present on all T lymphocytes and absent from all B lymphocytes, and it thus serves as a valuable T-cell marker (J:5243). It is very widely used in experimentsdesigned to determine the distribution and function of T-cells. Thy1 specifies a T-cell surface glycoprotein, T25, with a molecular weight of 25 kDa (J:5707). The protein appears to be anchored in the cell membrane by a lipid that is either phosphotidylinositol or closely related to it (J:12016). Thy1 may function in the cell membrane as a signal transduction molecule (J:8333). The Thy1 locus, or possibly a gene closely linked to it, controls quantitative expression of a protein that is the same size as Thy1 and is expressed on thymus and brain but not on lymph node and spleen cells (J:7900).

Gene Symbol and Name Ptprc, protein tyrosine phosphatase, receptor type, C
Chromosome 1
Gene Common Name(s) B220; CD45; CD45 antigen; CD45R; Cd45; GP180; L-CA; LCA; LY5; Ly-5; Lyt-4; RT7; T-lymphocyte antigen 4; T200; lymphocyte antigen 5;

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Kim I; Saunders TL; Morrison SJ. 2007. Sox17 dependence distingushes the transcriptional regulation of fetal from adult hematopoietic stem cells Cell 130(3):470-83. [PubMed: 17655922]  [MGI Ref ID J:123050]

Additional References

Thy1a related

Beck PL; Li Y; Wong J; Chen CW; Keenan CM; Sharkey KA; McCafferty DM. 2007. Inducible nitric oxide synthase from bone marrow-derived cells plays a critical role in regulating colonic inflammation. Gastroenterology 132(5):1778-90. [PubMed: 17449036]  [MGI Ref ID J:128325]

Chen TT; Li L; Chung DH; Allen CD; Torti SV; Torti FM; Cyster JG; Chen CY; Brodsky FM; Niemi EC; Nakamura MC; Seaman WE; Daws MR. 2005. TIM-2 is expressed on B cells and in liver and kidney and is a receptor for H-ferritin endocytosis. J Exp Med 202(7):955-65. [PubMed: 16203866]  [MGI Ref ID J:107466]

D'Eustachio P; Owens GC; Edelman GM; Cunningham BA. 1985. Chromosomal location of the gene encoding the neural cell adhesion molecule (N-CAM) in the mouse. Proc Natl Acad Sci U S A 82(22):7631-5. [PubMed: 3865183]  [MGI Ref ID J:8111]

Fife BT; Griffin MD; Abbas AK; Locksley RM; Bluestone JA. 2006. Inhibition of T cell activation and autoimmune diabetes using a B cell surface-linked CTLA-4 agonist. J Clin Invest 116(8):2252-61. [PubMed: 16886063]  [MGI Ref ID J:113109]

Green MC; Sweet HO. 1975. [Hx - Hm - W.] Mouse News Lett 52:38.  [MGI Ref ID J:13675]

Leiter EH. 1998. NOD Mice and Related Strains: Origins, Husbandry and Biology Introduction. In: NOD Mice and Related Strains: Research Applications in Diabetes, AIDS, Cancer, and Other Diseases. RG Landes, Austin.  [MGI Ref ID J:110093]

Ranheim EA; Tarbell KV; Krogsgaard M; Mallet-Designe V; Teyton L; McDevitt HO; Weissman IL. 2004. Selection of aberrant class II restricted CD8+ T cells in NOD mice expressing a glutamic acid decarboxylase (GAD)65-specific T cell receptor transgene. Autoimmunity 37(8):555-67. [PubMed: 15763918]  [MGI Ref ID J:128250]

Read S; Hogan TV; Zwar TD; Gleeson PA; Van Driel IR. 2007. Prevention of autoimmune gastritis in mice requires extra-thymic T-cell deletion and suppression by regulatory T cells. Gastroenterology 133(2):547-58. [PubMed: 17603058]  [MGI Ref ID J:128303]

Voehringer D; Liang HE; Locksley RM. 2008. Homeostasis and effector function of lymphopenia-induced 'memory-like' T cells in constitutively T cell-depleted mice. J Immunol 180(7):4742-53. [PubMed: 18354198]  [MGI Ref ID J:133382]

Wuthrich M; Warner T; Klein BS. 2005. IL-12 is required for induction but not maintenance of protective, memory responses to Blastomyces dermatitidis: implications for vaccine development in immune-deficient hosts. J Immunol 175(8):5288-97. [PubMed: 16210634]  [MGI Ref ID J:119110]

Xiao Z; Mescher MF; Jameson SC. 2007. Detuning CD8 T cells: down-regulation of CD8 expression, tetramer binding, and response during CTL activation. J Exp Med 204(11):2667-77. [PubMed: 17954566]  [MGI Ref ID J:126126]

Zaleski M; Klein J. 1974. Immune response of mice to Thy-1. 1 antigen: genetic control by alleles at the Ir-5 locus loosely linked to the H-2 complex. J Immunol 113(4):1170-7. [PubMed: 4606643]  [MGI Ref ID J:5487]

Zaleski MB. 1975. Immune response of mice to the Thy-1.1 antigen: effect of the Ir-5 alleles studies in 129/J and B10.129(6M) mice Immunogenetics 2:241-8.  [MGI Ref ID J:30773]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $1900.00
*Price(s) in US dollars ($)

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $2470.00
*Price(s) in US dollars ($)

Additional Supply Details

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • This strain is included in the Induced Mutant Resource Colony collection.

General Terms and Conditions


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