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| Mice that are homozygous for this SRY-box containing gene 17 targeted mutation are embryonic lethal around E13.5. Embryos exhibit growth retardation, show severe posterior body patterning abnormalities, have hematopoietic defects and lack definitive hematopoietic stem cells. Heterozygous and homozygous mice express EGFP in cells expressing this gene, therefore, these mice may be useful to understand the gene's activity in various cell types. This strain may be helpful in studies of fetal hematopoietic stem cells and hematopoiesis. | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Donating Investigator Sean J Morrison, UT Southwestern Description
Mice that are homozygous for this targeted mutation are embryonic lethal around E13.5. No gene product (mRNA) is detected by RT-PCR of whole homozygous embryos. Embryos exhibit growth retardation, show severe posterior body patterning abnormalities, have hematopoietic defects and lack definitive hematopoietic stem cells. Heterozygous and homozygous mice express EGFP in cells expressing this gene, therefore, these mice may be useful to understand the gene's activity in various cell types. This strain may be helpful in studies of fetal hematopoietic stem cells and hematopoiesis.Development
A targeting vector was designed to replace the coding region (involving exons 4 and 5) with EGFP and an FRT-flanked neomycin resistance cassette. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/Ka CD45.2:Thy1.1 (Ptprcb Thy1a) mice. A cross to a C57BL/6-background Actb-FLP strain was then carried out to remove the FRT-flanked neomycin cassette. The line was then backcrossed to the C57BL/Ka CD45.2:Thy1.1 line for eight generations by the donating laboratory. This strain is Line 1.
Related Strains
Strains carrying Ptprcb allele
017351 BKa.Cg-Ptprcb Bmi1tm1Ilw Thy1a/J 014149 NOD.B6-Ptprcb/6908MrkTacJ View Strains carrying Ptprcb (2 strains)
005895 B10.Cg-Thy1a H2d Tg(TcraCl1,TcrbCl1)1Shrm/J 001317 B6.Cg-Igha Thy1a Gpi1a/J 014550 B6.Cg-Thy1a Tg(TcraCWM5,TcrbCWM5)1807Wuth/J 005023 B6.Cg-Thy1a/Cy Tg(TcraTcrb)8Rest/J 000406 B6.PL-Thy1a/CyJ 000983 B6.PL/(84NS)CyJ 017351 BKa.Cg-Ptprcb Bmi1tm1Ilw Thy1a/J 007686 BKa.Cg-Sox17tm2Sjm Ptprcb Thy1a/J 005307 CBy.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ 005922 CBy.Cg-Thy1a Tg(TcraCl1,TcrbCl1)1Shrm/J 005443 CBy.PL(B6)-Thy1a/ScrJ 005686 NOD.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ 004483 NOD.NON-Thy1a/1LtJ 002721 NOD.NON-Thy1a/J 005651 SJL.AK-Thy1a/TseJ 003961 SJL.Cg Thy1a-Noxo1hslt/J
007172 129S6.129X1(B6)-Ptprctm1Weis/J 002725 B6.129-Ptprctm1Holm/J 008451 B6.129P(Cg)-Ptprca Cx3cr1tm1Litt/LittJ 007594 B6.129P2-Ptrpca Ightm1Mnz/J 007171 B6.129X1-Ptprctm1Weis/J 006259 B6.Cg-Pepcb Ptprca Tg(UBC-scFv)2Nemz/J 013198 B6.Cg-Ptprca Mir223tm1Fcam/J 002014 B6.SJL-Ptprca Pepcb/BoyJ 007686 BKa.Cg-Sox17tm2Sjm Ptprcb Thy1a/J 018869 C.129S4(Cg)-Il13tm2.1Lky/J 007173 C.129X1(B6)-Ptprctm1Weis/J 016091 C57BL/6-Ptprcltng/J 006584 CBy.SJL(B6)-Ptprca/J 005616 NOD.C-(Ptprc-D1Mit262)/WehiJ
007686 BKa.Cg-Sox17tm2Sjm Ptprcb Thy1a/J
Phenotype
Phenotype Information
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Hepatitis C Virus, Susceptibility to (PTPRC)
Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-Negative, B Cell-Positive, Nk Cell-Positive (PTPRC)
Vesicoureteral Reflux 3; VUR3 (SOX17)
assigned by genotype
Sox17tm1Sjm/Sox17tm1Sjm
BKa.129-Sox17tm1Sjm
- mortality/aging
- complete embryonic lethality during organogenesis
- expected numbers of homozygotes are found at E12.5, but complete lethality is observed by E13.5 (MGI Ref ID J:123050)
- embryogenesis phenotype
- abnormal embryo turning
- body axis rotation defects are observed by E11.5 (MGI Ref ID J:123050)
- abnormal embryonic hematopoiesis
- hematopoietic and erythroid cells are significantly reduced in the yolk sac and embryo at E11.5 (MGI Ref ID J:123050)
- abnormal rostral-caudal axis patterning
- at E11.5, embryos show severe defects in posterior patterning (MGI Ref ID J:123050)
- embryonic growth retardation
- mice exhibit severe growth retardation by E11.5 compared to controls (MGI Ref ID J:123050)
- hematopoietic system phenotype
- abnormal embryonic hematopoiesis
- hematopoietic and erythroid cells are significantly reduced in the yolk sac and embryo at E11.5 (MGI Ref ID J:123050)
- decreased hematopoietic stem cell number (MGI Ref ID J:123050)
- liver/biliary system phenotype
- liver hypoplasia
- at E12.5, livers of homozygotes are extremely hypocellular (MGI Ref ID J:123050)
- growth/size phenotype
- embryonic growth retardation
- mice exhibit severe growth retardation by E11.5 compared to controls (MGI Ref ID J:123050)
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Sox17tm1Sjm/Sox17tm1Sjm
involves: 129S1/Sv * 129X1/SvJ
- endocrine/exocrine gland phenotype
- abnormal bile duct development
- mice lack a distinguishable Ipfl (Pdx1)+ ventral bud and cells are scattered throughout the liver unlike in control mice (MGI Ref ID J:151981)
- absent pancreas
- at E9.0, mice lack a ventral pancreas (MGI Ref ID J:151981)
- liver/biliary system phenotype
- abnormal bile duct development
- mice lack a distinguishable Ipfl (Pdx1)+ ventral bud and cells are scattered throughout the liver unlike in control mice (MGI Ref ID J:151981)
This mouse can be used to support research in many areas including:
Thy1a relatedHematological Research
Hematopoietic Defects
Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Research Tools
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: T cell specific surface marker
Immunology and Inflammation Research
T cell specific surface marker
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Ptprcb | ||
|---|---|---|---|
| Allele Name | b variant | ||
| Allele Type | Not Applicable | ||
| Common Name(s) | CD45.2; Ly5b; | ||
| Gene Symbol and Name | Ptprc, protein tyrosine phosphatase, receptor type, C | ||
| Chromosome | 1 | ||
| Gene Common Name(s) | B220; CD45; CD45 antigen; CD45R; Cd45; GP180; L-CA; LCA; LY5; Ly-5; Lyt-4; RT7; T-lymphocyte antigen 4; T200; lymphocyte antigen 5; | ||
| Molecular Note |
Ptprcb is found in strains C57BL/6, C3H/An, DBA/2, AKR, and many others (J:13367, J:12054, J:12077, J:8603). Twelve nucleotide differences between the a and b alleles have been identified. These base substitutions correspond to five amino-acidchanges within the extracellular domain of the encoded protein. These amino-acid differences are clustered in a region that also contains the greatest divergence between mouse and rat sequences (J:22485). Note that the allele designations originally described were reversed in 1987 (J:8603); all publications prior to 1987 show SJL/J, STS/A, and DA as having the b allele and the C57BL/6J group as having the a allele (J:22341). [MGI Ref ID J:12548] [MGI Ref ID J:22485] [MGI Ref ID J:8080] | ||
| Allele Symbol | Sox17tm1Sjm | ||
| Allele Name | targeted mutation 1, Sean J Morrison | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | Sox17GFP; | ||
| Mutation Made By | Sean Morrison, UT Southwestern | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Gene Symbol and Name | Sox17, SRY-box containing gene 17 | ||
| Chromosome | 1 | ||
| Gene Common Name(s) | VUR3; | ||
| Molecular Note | A targeting vector was designed to replace the coding region (involving exons 4 and 5) in frame with the Sox17 start codon, with EGFP and an FRT-flanked neomycin resistance cassette. No Sox17 mRNA is detected by RT-PCR. EGFP is expressed in cells that normally express Sox17. [MGI Ref ID J:123050] | ||
| Allele Symbol | Thy1a | ||
| Allele Name | a variant | ||
| Allele Type | Not Applicable | ||
| Common Name(s) | Thy-1.1; Thy1.1; Thy1a; theta-AKR; thetaAKR; | ||
| Site of Expression | The Thy1 locus determines a surface antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells. | ||
| Gene Symbol and Name | Thy1, thymus cell antigen 1, theta | ||
| Chromosome | 9 | ||
| Gene Common Name(s) | CD7; CD90; T25; Thy 1.2; Thy-1; Thy-1.2; Thy1.1; Thy1.2; theta; | ||
| General Note |
The Thy1 locus determines a surface antigen present on cells of the thymus, a number of mouse leukemias, brain, and in lesser amounts on lymph node and spleen cells. The allele Thy1a determines an antigenic specificity, Thy-1.1, found in the AKR and RF strains; the allele Thy1b determines an antigenic specificity, Thy-1.2, found in the C3HeB/Fe and many other strains (J:5243, J:5012, J:4469). The Thy1 antigen is probably present on all T lymphocytes and absent from all B lymphocytes, and it thus serves as a valuable T-cell marker (J:5243). It is very widely used in experiments designed to determine the distribution and function of T-cells. Thy1 specifies a T-cell surface glycoprotein, T25, with a molecular weight of 25 kDa (J:5707). The protein appears to be anchored in the cell membrane by a lipid that is either phosphotidylinositol or closely related to it (J:12016). Thy1 may function in the cell membrane as a signal transduction molecule (J:8333). The Thy1 locus, or possibly a gene closely linked to it, controls quantitative expression of a protein that isthe same size as Thy1 and is expressed on thymus and brain but not on lymph node and spleen cells (J:7900). | ||
| Molecular Note | The allele Thy1a determines an antigenic specificity, Thy-1.1, found in the AKR and RF strains. | ||
Genotyping
Genotyping Information
Genotyping Protocols
Sox17tm1Sjm, Fast MCA
Sox17tm1Sjm, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Kim I; Saunders TL; Morrison SJ. 2007. Sox17 dependence distingushes the transcriptional regulation of fetal from adult hematopoietic stem cells Cell 130(3):470-83. [PubMed: 17655922] [MGI Ref ID J:123050]
Additional References
Ptprcb relatedSox17tm1Sjm relatedEwald SJ; McKenzie IFC. 1979. Immunochemical characterization of the Ly-5 alloantigens on thymocytes. Immunogenetics 8:583-587. [MGI Ref ID J:13367]
Harp JA; Davis BS; Ewald SJ. 1984. Inhibition of T cell responses to alloantigens and polyclonal mitogens by Ly-5 antisera. J Immunol 133(1):10-5. [PubMed: 6233370] [MGI Ref ID J:7453]
Held W; Clevers H; Grosschedl R. 2003. Redundant functions of TCF-1 and LEF-1 during T and NK cell development, but unique role of TCF-1 for Ly49 NK cell receptor acquisition. Eur J Immunol 33(5):1393-8. [PubMed: 12731066] [MGI Ref ID J:85394]
Kasai M; Leclerc JC; Shen FW; Cantor H. 1979. Identification of Ly 5 on the surface of `natural killer' cells in normal and athymic inbred mouse strains. Immunogenetics 8:153-159. [MGI Ref ID J:12054]
Komuro K ; Itakura K ; Boyse EA ; John M. 1975. Ly-5: a new lymphocyte antigen system. Immunogenetics 1:452-456. [MGI Ref ID J:12077]
Mobraaten LE. 1994. Ly5 gene nomenclature, C57BL/6J and SJL/J - A history of change JAX Notes Summer(458):2. [MGI Ref ID J:22341]
Morse HC 3rd; Shen FW; Hammerling U. 1987. Genetic nomenclature for loci controlling mouse lymphocyte antigens. Immunogenetics 25(2):71-8. [PubMed: 3817907] [MGI Ref ID J:8603]
Raschke WC; Hendricks M; Chen CM. 1995. Genetic basis of antigenic differences between three alleles of Ly5 (CD45) in mice. Immunogenetics 41(2-3):144-7. [PubMed: 7806287] [MGI Ref ID J:22485]
Seldin MF; D'Hoostelaere LA; Steinberg AD; Saga Y; Morse HC 3rd. 1987. Allelic variants of Ly-5 in inbred and natural populations of mice. Immunogenetics 26(1-2):74-8. [PubMed: 2886425] [MGI Ref ID J:8805]
Shen FW; Saga Y; Litman G; Freeman G; Tung JS; Cantor H; Boyse EA. 1985. Cloning of Ly-5 cDNA. Proc Natl Acad Sci U S A 82(21):7360-3. [PubMed: 3864163] [MGI Ref ID J:8080]
Shen FW; Tung JS; Boyse EA. 1986. Further definition of the Ly-5 system. Immunogenetics 24(3):146-9. [PubMed: 3489673] [MGI Ref ID J:8430]
Waterstrat A; Liang Y; Swiderski CF; Shelton BJ; Van Zant G. 2010. Congenic interval of CD45/Ly-5 congenic mice contains multiple genes that may influence hematopoietic stem cell engraftment. Blood 115(2):408-17. [PubMed: 19901263] [MGI Ref ID J:155980]
Yeager AM; Shinn C; Shinohara M; Pardoll DM. 1993. Hematopoietic cell transplantation in the twitcher mouse. The effects of pretransplant conditioning with graded doses of busulfan. Transplantation 56(1):185-90. [PubMed: 8101401] [MGI Ref ID J:14381]
Zebedee SL; Barritt DS; Raschke WC. 1991. Comparison of mouse Ly5a and Ly5b leucocyte common antigen alleles. Dev Immunol 1(4):243-54. [PubMed: 1822988] [MGI Ref ID J:12548]
Thy1a relatedNiakan KK; Ji H; Maehr R; Vokes SA; Rodolfa KT; Sherwood RI; Yamaki M; Dimos JT; Chen AE; Melton DA; McMahon AP; Eggan K. 2010. Sox17 promotes differentiation in mouse embryonic stem cells by directly regulating extraembryonic gene expression and indirectly antagonizing self-renewal. Genes Dev 24(3):312-26. [PubMed: 20123909] [MGI Ref ID J:156932]
Saund RS; Kanai-Azuma M; Kanai Y; Kim I; Lucero MT; Saijoh Y. 2012. Gut endoderm is involved in the transfer of left-right asymmetry from the node to the lateral plate mesoderm in the mouse embryo. Development 139(13):2426-35. [PubMed: 22627279] [MGI Ref ID J:185535]
Spence JR; Lange AW; Lin SC; Kaestner KH; Lowy AM; Kim I; Whitsett JA; Wells JM. 2009. Sox17 regulates organ lineage segregation of ventral foregut progenitor cells. Dev Cell 17(1):62-74. [PubMed: 19619492] [MGI Ref ID J:151981]
Yang H; Lee S; Lee S; Kim K; Yang Y; Kim JH; Adams RH; Wells JM; Morrison SJ; Koh GY; Kim I. 2013. Sox17 promotes tumor angiogenesis and destabilizes tumor vessels in mice. J Clin Invest 123(1):418-31. [PubMed: 23241958] [MGI Ref ID J:194296]
Beck PL; Li Y; Wong J; Chen CW; Keenan CM; Sharkey KA; McCafferty DM. 2007. Inducible nitric oxide synthase from bone marrow-derived cells plays a critical role in regulating colonic inflammation. Gastroenterology 132(5):1778-90. [PubMed: 17449036] [MGI Ref ID J:128325]
Chen TT; Li L; Chung DH; Allen CD; Torti SV; Torti FM; Cyster JG; Chen CY; Brodsky FM; Niemi EC; Nakamura MC; Seaman WE; Daws MR. 2005. TIM-2 is expressed on B cells and in liver and kidney and is a receptor for H-ferritin endocytosis. J Exp Med 202(7):955-65. [PubMed: 16203866] [MGI Ref ID J:107466]
Cuda CM; Wan S; Sobel ES; Croker BP; Morel L. 2007. Murine lupus susceptibility locus Sle1a controls regulatory T cell number and function through multiple mechanisms. J Immunol 179(11):7439-47. [PubMed: 18025188] [MGI Ref ID J:154964]
D'Eustachio P; Owens GC; Edelman GM; Cunningham BA. 1985. Chromosomal location of the gene encoding the neural cell adhesion molecule (N-CAM) in the mouse. Proc Natl Acad Sci U S A 82(22):7631-5. [PubMed: 3865183] [MGI Ref ID J:8111]
Dewals B; Hoving JC; Horsnell WG; Brombacher F. 2010. Control of Schistosoma mansoni egg-induced inflammation by IL-4-responsive CD4(+)CD25(-)CD103(+)Foxp3(-) cells is IL-10-dependent. Eur J Immunol 40(10):2837-47. [PubMed: 20821727] [MGI Ref ID J:165816]
Divangahi M; Desjardins D; Nunes-Alves C; Remold HG; Behar SM. 2010. Eicosanoid pathways regulate adaptive immunity to Mycobacterium tuberculosis. Nat Immunol 11(8):751-8. [PubMed: 20622882] [MGI Ref ID J:162390]
Dolfi DV; Duttagupta PA; Boesteanu AC; Mueller YM; Oliai CH; Borowski AB; Katsikis PD. 2011. Dendritic cells and CD28 costimulation are required to sustain virus-specific CD8+ T cell responses during the effector phase in vivo. J Immunol 186(8):4599-608. [PubMed: 21389258] [MGI Ref ID J:172460]
Fife BT; Griffin MD; Abbas AK; Locksley RM; Bluestone JA. 2006. Inhibition of T cell activation and autoimmune diabetes using a B cell surface-linked CTLA-4 agonist. J Clin Invest 116(8):2252-61. [PubMed: 16886063] [MGI Ref ID J:113109]
Green MC; Sweet HO. 1975. [Hx - Hm - W.] Mouse News Lett 52:38. [MGI Ref ID J:13675]
Inlay MA; Bhattacharya D; Sahoo D; Serwold T; Seita J; Karsunky H; Plevritis SK; Dill DL; Weissman IL. 2009. Ly6d marks the earliest stage of B-cell specification and identifies the branchpoint between B-cell and T-cell development. Genes Dev 23(20):2376-81. [PubMed: 19833765] [MGI Ref ID J:154864]
Kelly LM; Pereira JP; Yi T; Xu Y; Cyster JG. 2011. EBI2 guides serial movements of activated B cells and ligand activity is detectable in lymphoid and nonlymphoid tissues. J Immunol 187(6):3026-32. [PubMed: 21844396] [MGI Ref ID J:179238]
Krieg C; Letourneau S; Pantaleo G; Boyman O. 2010. Improved IL-2 immunotherapy by selective stimulation of IL-2 receptors on lymphocytes and endothelial cells. Proc Natl Acad Sci U S A 107(26):11906-11. [PubMed: 20547866] [MGI Ref ID J:161365]
Leiter EH. 1998. NOD Mice and Related Strains: Origins, Husbandry and Biology Introduction. In: NOD Mice and Related Strains: Research Applications in Diabetes, AIDS, Cancer, and Other Diseases. RG Landes, Austin. [MGI Ref ID J:110093]
REIF AE; ALLEN JM. 1964. THE AKR THYMIC ANTIGEN AND ITS DISTRIBUTION IN LEUKEMIAS AND NERVOUS TISSUES. J Exp Med 120:413-33. [PubMed: 14207060] [MGI Ref ID J:24839]
Ranheim EA; Tarbell KV; Krogsgaard M; Mallet-Designe V; Teyton L; McDevitt HO; Weissman IL. 2004. Selection of aberrant class II restricted CD8+ T cells in NOD mice expressing a glutamic acid decarboxylase (GAD)65-specific T cell receptor transgene. Autoimmunity 37(8):555-67. [PubMed: 15763918] [MGI Ref ID J:128250]
Read S; Hogan TV; Zwar TD; Gleeson PA; Van Driel IR. 2007. Prevention of autoimmune gastritis in mice requires extra-thymic T-cell deletion and suppression by regulatory T cells. Gastroenterology 133(2):547-58. [PubMed: 17603058] [MGI Ref ID J:128303]
Reif AE; Allen JM. 1966. Mouse thymic iso-antigens. Nature 209(22):521-3. [PubMed: 5919593] [MGI Ref ID J:5012]
Sercan O; Stoycheva D; Hammerling GJ; Arnold B; Schuler T. 2010. IFN-gamma receptor signaling regulates memory CD8+ T cell differentiation. J Immunol 184(6):2855-62. [PubMed: 20164422] [MGI Ref ID J:160112]
Uhl M; Kepp O; Jusforgues-Saklani H; Vicencio JM; Kroemer G; Albert ML. 2009. Autophagy within the antigen donor cell facilitates efficient antigen cross-priming of virus-specific CD8+ T cells. Cell Death Differ 16(7):991-1005. [PubMed: 19229247] [MGI Ref ID J:164191]
Voehringer D; Liang HE; Locksley RM. 2008. Homeostasis and effector function of lymphopenia-induced 'memory-like' T cells in constitutively T cell-depleted mice. J Immunol 180(7):4742-53. [PubMed: 18354198] [MGI Ref ID J:133382]
Wang L; Jameson SC; Hogquist KA. 2009. Epidermal Langerhans cells are not required for UV-induced immunosuppression. J Immunol 183(9):5548-53. [PubMed: 19843938] [MGI Ref ID J:156799]
Wuthrich M; Ersland K; Pick-Jacobs JC; Gern BH; Frye CA; Sullivan TD; Brennan MB; Filutowicz HI; O'Brien K; Korthauer KD; Schultz-Cherry S; Klein BS. 2012. Limited model antigen expression by transgenic fungi induces disparate fates during differentiation of adoptively transferred T cell receptor transgenic CD4+ T cells: robust activation and proliferation with weak effector function during recall. Infect Immun 80(2):787-97. [PubMed: 22124658] [MGI Ref ID J:180817]
Wuthrich M; Warner T; Klein BS. 2005. IL-12 is required for induction but not maintenance of protective, memory responses to Blastomyces dermatitidis: implications for vaccine development in immune-deficient hosts. J Immunol 175(8):5288-97. [PubMed: 16210634] [MGI Ref ID J:119110]
Xiao Z; Mescher MF; Jameson SC. 2007. Detuning CD8 T cells: down-regulation of CD8 expression, tetramer binding, and response during CTL activation. J Exp Med 204(11):2667-77. [PubMed: 17954566] [MGI Ref ID J:126126]
Yu Y; Cho HI; Wang D; Kaosaard K; Anasetti C; Celis E; Yu XZ. 2013. Adoptive Transfer of Tc1 or Tc17 Cells Elicits Antitumor Immunity against Established Melanoma through Distinct Mechanisms. J Immunol 190(4):1873-81. [PubMed: 23315072] [MGI Ref ID J:193239]
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Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2450.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $3185.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.