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| These mutant mice contain a beta-galactosidase gene, lacZ, inserted into the Gt(ROSA)26Sor locus. Expression of the lacZ gene is blocked by a attB and attP site flanked STOP fragment placed between the lacZ sequence and the Gt(ROSA)26Sor promoter. This strain serves as a reporter strain, with successful PhiC31o recombination being indicated by beta-galactosidase expression in PhiC31o-expressing tissues. Mice that are homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. | |||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names 129S4-Gt(ROSA)26Sortm4(attB/attP)Sor/J (Changed: 17-JAN-08 ) Type Gene Trap; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Generation N2?+F1 (08-JAN-08) Donating Investigator Philippe Soriano, Fred Hutchinson Cancer Research Center Description
These mutant mice contain a beta-galactosidase gene, lacZ, inserted into the Gt(ROSA)26Sor locus. Expression of the lacZ gene is blocked by a attB and attP site flanked STOP fragment placed between the lacZ sequence and the Gt(ROSA)26Sor promoter. This strain serves as a reporter strain, with successful PhiC31o recombination being indicated by beta-galactosidase expression in PhiC31o-expressing tissues. Mice that are homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.Development
A targeting construct with the SA-attB-PGKneo 4xpA-attP-lacZ-pA cassette, in which the STOP fragment is flanked by attB and attP sites, was inserted into the Gt(ROSA)26Sor locus via electroporation of 129S4/SvJaeSor-derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were identified and used to generate mutant mice. The mice were crossed to 129S4/SvJaeSor mice for 2 generations prior to their arrival at The Jackson Laboratory and then intercrossed to generate homozygotes.
Control Information
| Control | ||
|---|---|---|
| 002448 129S1/SvImJ | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Gt(ROSA)26Sor
View Strains carrying other alleles of Gt(ROSA)26Sor (47 strains)
Phenotype
Phenotype Information
assigned by genotype
Gt(ROSA)26Sortm4(attB/attP)Sor/Gt(ROSA)26Sortm4(attB/attP)Sor
involves: 129S4/SvJaeSor
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:128069)
- homozygotes are viable, fertile, and show no gross physical or behavioral abnormalities
This mouse can be used to support research in many areas including:
Research Tools
PhiC31 System (attB/attP-flanked Sequences: Test/Reporter)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Gt(ROSA)26Sortm4(attB/attP)Sor | ||
|---|---|---|---|
| Allele Name | targeted mutation 4, Philippe Soriano | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | R26attR; | ||
| Strain of Origin | 129S4/SvJaeSor | ||
| ES Cell Line Name | AK7 | ||
| ES Cell Line Strain | 129S4/SvJaeSor | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| Molecular Note | A targeting construct with the SA-attB-PGKneo 4xpA-attP-lacZ-pA cassette, in which the STOP fragment is flanked by attB and attP sites (phi31 recognition sites), was inserted into the Gt(ROSA)26Sor. Expression of the lacZ gene is blocked by a attB and attP site flanked STOP fragment placed between the lacZ sequence and the Gt(ROSA)26Sor promoter. PhiC31o-mediated recombination will excise the STOP and allow lacZ transcription. [MGI Ref ID J:128069] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sor tm3(phiC31*)Sor, tm4(attB/attP)Sor, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Raymond CS; Soriano P. 2007. High-efficiency FLP and PhiC31 site-specific recombination in mammalian cells. PLoS ONE 2(1):e162. [PubMed: 17225864] [MGI Ref ID J:128069]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as homozygotes. Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $155.70 Female or Male Homozygous for Gt(ROSA)26Sortm4(attB/attP)Sor *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $311.40 Homozygous for Gt(ROSA)26Sortm4(attB/attP)Sor x Homozygous for Gt(ROSA)26Sortm4(attB/attP)Sor
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $202.50 Female or Male Homozygous for Gt(ROSA)26Sortm4(attB/attP)Sor *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $404.90 Homozygous for Gt(ROSA)26Sortm4(attB/attP)Sor x Homozygous for Gt(ROSA)26Sortm4(attB/attP)Sor
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 002448 129S1/SvImJ | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
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