Strain Name: |
C57BL/6-Tg(APOE-DGAT2)24Far/J |
|---|---|
Stock Number: |
007744 |
Availability: | Under Development for Distribution Colony |
| To register your interest in this strain go to the Strain Interest Form. |
General Terms and Conditions |
| Genes & Alleles | APOE; DGAT2; Tg(APOE-DGAT2)24Far; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Coisogenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Robert Farese, Jr., Gladstone Institute UCSF Generation F?+N1+ (27-AUG-08) Strain Description
Mice hemizygous for this LivLE6-DGAT2 transgene are viable and fertile, with human DGAT2 expression directed to the liver by the hepatic promoter/enhancer sequences from the human apolipoprotein E gene. Mice from founder line 24 (referred to as Liv-DGAT2-low) have an approximately 2-fold increase in total hepatic DGAT2 mRNA/protein expression, with no reported overexpression in kidney, brain, or skeletal muscle. As DGAT2 is one of two enzymes that catalyze the final step of triacylglycerol (TG) biosynthesis, transgenic mice develop hepatic steatosis with increased hepatic TG and insulin signaling lipid (diacylglycerol, ceramide and unsaturated Fatty AcylCoA) content. Liv-DGAT2-low mice also exhibit increased transcription of fatty acid synthesis genes (SREBP-1c, fatty acid synthase, and stearoyl CoA desaturase 1). Fasted mice show a 65% decrease in plasma TG, but are not insulin resistant (blood glucose and insulin are similar to wildtype). Liv-DGAT2-low mice challenged with a high-fat diet do not exhibit impaired glucose or insulin tolerance more than that found in control mice. These Liv-DGAT2-low transgenic mice may be useful in studying hepatic steatosis, lipid, glucose, and insulin metabolism, as well as obesity and diabetes.Of note, these mice may also be useful in conjunction with MCK-DGAT2 transgenic mice (Stock No. 006781) which exhibit DGAT2 overexpression directed to glycolytic striated skeletal muscle.
Strain Development
The LivLE6-DGAT2 transgene was designed with promoter and enhancer sequences of the human apolipoprotein E gene placed upstream of a 1.2 kb human acyl-CoA:diacylglycerol acyltransferase 2 (DGAT2) cDNA sequence. This transgene was microinjected into C57BL/6NHsd fertilized eggs, and the resulting transgenic offspring were bred to C57BL/6NHsd. Founder line 24 (Liv-DGAT2-low) mice were established and maintained by breeding transgenic mice to C57BL/6NHsd for many generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred with C57BL/6NJ (Stock No. 005304) to establish the colony.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(APOE-DGAT2)24Far | ||
|---|---|---|---|
| Allele Name | transgene insertion 24, Bob Farese | ||
| Common Name(s) | Liv-DGAT2-low; LivLe6-DGAT2 (low); | ||
| Mutation Made By | Mara Monetti, Gladstone Institute UCSF | ||
| Strain of Origin | C57BL/6NHsd | ||
| Expressed Gene | DGAT2, diacylglycerol O-acyltransferase homolog 2 (mouse), human | ||
| Promoter | APOE, apolipoprotein E, human | ||
| Molecular Note | The LivLE6-DGAT2 transgene was designed with promoter and enhancer sequences of the human apolipoprotein E gene placed upstream of a 1.2 kb human acyl-CoA:diacylglycerol acyltransferase 2 (DGAT2) cDNA sequence. This transgene was microinjected into C57BL/6NHsd fertilized eggs, and the resulting transgenic offspring were bred to C57BL/6NHsd. Mice from founder line 24 (referred to as Liv-DGAT2-low) have an approximately 2-fold increase in total hepatic DGAT2 mRNA/protein expression, with no reported overexpression in kidney, brain, or skeletal muscle. [MGI Ref ID J:124005] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Please Inquire | (approximate) Stock No. 005304 (C57BL/6NJ) is an approximate control. | |
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(APOE-DGAT2)24Far
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, transgenic mice can be bred to wildtype siblings. Alternatively, transgenic mice may be bred together, although a homozygous phenotype is not yet characterized (Jul-2007). |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of APOE
004632 B6.Cg-Tg(GFAP-APOE*2)14Hol Apoetm1Unc/J 004631 B6.Cg-Tg(GFAP-APOE*4)1Hol Apoetm1Unc/J 008408 B6;D2-Tg(APOE-NPC1L1)20Lqyu/J 003285 C57BL/6-Tg(LIPC)6784His/J View Strains carrying other alleles of APOE (4 strains)
006781 C57BL/6-Tg(Ckm-DGAT2)10Far/J
Research Applications
This mouse can be used to support research in many areas including:
APOE relatedCardiovascular Research
Hypertriglyceridemia
Other (altered fat metabolism)
Other (altered lipoprotein profile)
Internal/Organ Research
Liver Defects
Metabolism Research
Lipid Metabolism
Research Tools
Cardiovascular Research
Diabetes and Obesity Research
Internal/Organ Research
Metabolism Research
Mouse/Human Gene Homologs
Alzheimer's
References
Selected Reference(s)
Monetti M; Levin MC; Watt MJ; Sajan MP; Marmor S; Hubbard BK; Stevens RD; Bain JR; Newgard CB; Farese RV Sr; Hevener AL; Farese RV Jr. 2007. Dissociation of hepatic steatosis and insulin resistance in mice overexpressing DGAT in the liver. Cell Metab 6(1):69-78. [PubMed: 17618857] [MGI Ref ID J:124005]
Price and Supply Information
| Strain Name: | C57BL/6-Tg(APOE-DGAT2)24Far/J |
| Stock Number: | 007744 |
This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date: 06-OCT-08
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development
Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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