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| These bic/mir-155 mutant mice may be useful in mircoRNA biology, specifically to study the role of miR-155 and its target genes (including cytokines, chemokines, and transcription factors) in homeostasis and function of the immune system. | |||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Heterozygote x Heterozygote (Female x Male) Species laboratory mouse Generation N6+F1 (29-SEP-08) Donating Investigator Klaus Rajewsky, Immune Disease Institute (formerly CBRI) Description
Mice homozygous for this loss-of-function/reporter allele (bic/mir-155-/-) are viable and fertile. The lacZ reporter allows the detection of bic promoter transcriptional activity using fluorescence activated cell sorting (FACS). In homozygotes, miR-155 expression is undetectable in activated splenic B cells. In heterozygotes, approximately 60% of germinal center (GC) B cells express the lacZ reporter whereas the vast majority of the non-GC B cells do not. Homozygous mice exhibit a reduced fraction of GC B cells in the gut-associated lymphoid tissue (GALT; including Peyer's patches (PPs) and mesenteric lymph nodes (mLNs)). In addition, bic/miR-155-/- B cells exhibit deficient tumor necrosis factor (TNF) and lymphotoxin-a (LT-a) cytokine production. Homozygous mice show impaired T cell-dependent antibody responses, and their T cells show a TH2 cytokine bias (an increased percentage of interleukin-4 (IL-4) producing cells and a decreased percentage of interferon-g (IFN-g) producing cells), accompanied by a higher fraction of cells producing IL-10. These bic/mir-155 mutant mice may be useful in mircoRNA biology, specifically to study the role of miR-155 and its target genes (including cytokines, chemokines, and transcription factors) in homeostasis and immune system function.Development
A modified bacterial artificial chromosome (BAC) targeting vector was used to replace a 0.97 kb portion of exon 2 of the bic/mir-155 gene with an in-frame b-galactosidase (lacZ) reporter gene (with polyA sequence and followed by a loxP-flanked neomycin resistance cassette). The construct was electroporated into F1H4 embryonic stem (ES) cells (derived from C57BL/6:129 hybrid mice). Chimera's were bred to C57BL/6 inbred mice to establish mutant mice. These mice were bred with a cre-deleter strain (congenic C57BL/6 background) to remove the loxP-flanked selection cassette, and the resulting bic/mir-155 mutant mice (with lacZ followed by a single remaining loxP site) were then backcrossed to C57BL/6 for at least 5 generations (selecting against the cre expressing mutation) prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (175 strains)
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Mirn155tm1.1Rsky/Mirn155tm1.1Rsky
involves: 129 * C57BL/6
- immune system phenotype
- abnormal B cell physiology (MGI Ref ID J:121084)
- abnormal germinal center B cell physiology (MGI Ref ID J:121084)
- B cells exhibit deficient cytokine production
- abnormal T cell physiology (MGI Ref ID J:121084)
- T cells show a Th2 cytokine bias (increased Il4-producing and decreased IFNgamma-producing cell numbers) with a higher fraction of interleukin-10 producing cells
- abnormal cytokine secretion (MGI Ref ID J:121084)
- B cells exhibit deficient TNFalpha and lymphotoxin A (LT-a) cytokine production
- abnormal humoral immune response (MGI Ref ID J:121084)
- mice show impaired T cell-dependent antibody responses
- decreased germinal center B cell number (MGI Ref ID J:121084)
- homozygous mice exhibit reduced numbers of germinal center B cells in gut-associated lymphoid tissue (GALT), including Peyer's patches and mesenteric lymph nodes
- hematopoietic system phenotype
- decreased germinal center B cell number (MGI Ref ID J:121084)
- homozygous mice exhibit reduced numbers of germinal center B cells in gut-associated lymphoid tissue (GALT), including Peyer's patches and mesenteric lymph nodes
This mouse can be used to support research in many areas including:
Cancer Research
Growth Factors/Receptors/Cytokines
Cell Biology Research
Transcriptional Regulation
Hematological Research
Immunological Defects (B and T cell deficiency)
Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Immunodeficiency (B and T cell deficiency)
Immunodeficiency (B cell defects)
Immunodeficiency (B cell deficiency)
Inflammation (B and T cell deficiency)
Lymphoid Tissue Defects (B and T cell deficiency)
Internal/Organ Research
Lymphoid Tissue Defects (B and T cell deficiency)
Lymphoid Tissue Defects (T cell deficiency)
Research Tools
lacZ Expression
Cancer Research (B cell deficiency)
Cancer Research (T cell deficiency)
Cancer Research (production of B and T cells, antibodies, and hybridomas)
Cancer Research (production of T cells and hybridoma)
Genetics Research (Mutagenesis and Transgenesis: transcriptional activation)
Immunology and Inflammation Research (B and T cell deficiency)
Immunology and Inflammation Research (B cell deficiency)
Immunology and Inflammation Research (T cell deficiency)
Immunology and Inflammation Research (production of B cells and antibodies)
Immunology and Inflammation Research (production of B cells, antibodies T cell lines, and hybridomas)
Immunology and Inflammation Research (production of T cell lines and hybridomas)
Virology Research
B and T Cell Deficiency
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Mirn155tm1.1Rsky | ||
|---|---|---|---|
| Allele Name | targeted mutation 1.1, Klaus Rajewsky | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | bic/miR-155-; | ||
| Mutation Made By | Klaus Rajewsky, Immune Disease Institute (formerly CBRI) | ||
| Strain of Origin | (129 x C57BL/6)F1 | ||
| ES Cell Line Name | F1H4 | ||
| ES Cell Line Strain | (129 x C57BL/6)F1 | ||
| Site of Expression | Approximately 60% of germinal center (GC) B cells express the lacZ reporter whereas the vast majority of the non-GC B cells do not. | ||
| Gene Symbol and Name | Mirn155, microRNA 155 | ||
| Chromosome | 16 | ||
| Gene Common Name(s) | mmu-mir-155; | ||
| Molecular Note | A modified bacterial artificial chromosome (BAC) targeting vector was used to replace a 0.97 kb portion of exon 2 of the bic/mir-155 gene with an in-frame b-galactosidase (lacZ) reporter gene (with polyA sequence and followed by a loxP-flanked neomycin resistance cassette). The mice were bred with a cre-deleter strain (congenic C57BL/6 background) to remove the loxP-flanked selection cassette. The resulting bic/mir-155 mutant mice (with lacZ followed by a single remaining loxP site). In heterozygotes, approximately 60% of germinal center (GC) B cells express the lacZ reporter whereas the vast majority of the non-GC B cells do not. [MGI Ref ID J:121084] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Mirn155tm1.1Rsky, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Thai TH; Calado DP; Casola S; Ansel KM; Xiao C; Xue Y; Murphy A; Frendewey D; Valenzuela D; Kutok JL; Schmidt-Supprian M; Rajewsky N; Yancopoulos G; Rao A; Rajewsky K. 2007. Regulation of the germinal center response by microRNA-155. Science 316(5824):604-8. [PubMed: 17463289] [MGI Ref ID J:121084]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred together. Mating System Heterozygote x Heterozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date: 01-DEC-08
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development and On Hold
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $236.40 Female or Male Heterozygous for Mirn155tm1.1Rsky *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $472.80 Heterozygous for Mirn155tm1.1Rsky x Heterozygous for Mirn155tm1.1Rsky
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $307.40 Female or Male Heterozygous for Mirn155tm1.1Rsky *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $614.70 Heterozygous for Mirn155tm1.1Rsky x Heterozygous for Mirn155tm1.1Rsky
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Under Development for Distribution Colony |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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