Description

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Heterozygote x Heterozygote         (Female x Male) Species laboratory mouse   Donating Investigator Klaus Rajewsky,   Immune Disease Institute (formerly CBRI)

Description
Mice homozygous for this targeted allele (miR150^-/-) are viable and fertile with normal development of T cells, follicular B cells, and MZ B cells. No miR-150 is expressed in spleen, mesenteric lymph node, and thymus of homozygotes. Homozygous mice exhibit B cell expansion (CD19⁺B220loCD5⁺CD43⁺CD23^-; B1a subset) in spleen and peritoneal cavity (with reciprocal reduction in B2 cells) and enhanced humoral immune response (increased serum immunoglobulins of various classes both at steady-state and following T cell-dependent antigen exposure). Homozygous miR-150 deficiency also leads to enhanced induction of the miR-150 target protein c-Myb in activated B and T cells, but no reported change in expression of the miR-150 target genes Foxp1 or ZFP91 in resting or activated B cells. These miR150^-/- mice may be useful in mircoRNA biology, specifically to study the role of miR-150 and its target genes (including c-Myb) in lymphocyte development and function.

Development
A targeting vector was designed to insert an frt-flanked pGK-Neo cassette 468 bp upstream of the miR-150 stem loop, with one loxP site placed immediately upstream of the pGK-Neo cassette and the other loxP site placed 146 bp downstream of the miR-150 stem loop. The construct was electroporated into C57BL/6-derived Bruce-4 embryonic stem (ES) cells. Chimeric mice were bred to C57BL/6 mice. To delete the loxP site-flanked 679 bp miR-150 coding genomic region and the frt-flanked pGK-Neo cassette, mice were then bred to cre-expressing mice (C57BL/6 congenic background, similar to Stock No. 006054). The resulting mice, with a single loxP site replacing the miR-150 coding region, were bred together (selecting against the cre transgene) to obtain miR150^-/- homozygotes.

Control Information

	Control
	000664 C57BL/6J
	100007 CB6F1/J	(approximate)
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Mirn150^tm1Rsky/Mirn150^tm1Rsky

        involves: C57BL/6

• immune system phenotype
• decreased B-2 B cell number (MGI Ref ID J:127234)
  • there is a 2-fold decrease in peritoneal B-2 B cell numbers
• increased B-1 B cell number (MGI Ref ID J:127234)
  • splenic B-1 cells are increased by 4-fold, whereas in the peritoneal cavity, there are 2-fold more B-1 B cell than in controls
• increased IgA level (MGI Ref ID J:127234)
  • a 9-fold increase in serum levels is seen
• increased IgG1 level (MGI Ref ID J:127234)
  • 3-fold increase in serum levels is seen
  • upon immunization with a T-cell dependent antigen, mice produce 3-4 times more IgG1 antibodies than controls
• increased IgG2b level (MGI Ref ID J:127234)
  • 4-fold increase in serum levels is seen
• increased IgM level (MGI Ref ID J:127234)
  • 2-fold increase in serum level is seen
• hematopoietic system phenotype
• decreased B-2 B cell number (MGI Ref ID J:127234)
  • there is a 2-fold decrease in peritoneal B-2 B cell numbers
• increased B-1 B cell number (MGI Ref ID J:127234)
  • splenic B-1 cells are increased by 4-fold, whereas in the peritoneal cavity, there are 2-fold more B-1 B cell than in controls

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cell Biology Research
Transcriptional Regulation

Hematological Research
Immunological Defects

Immunology and Inflammation Research
Immunodeficiency (B cell defects)
Immunodeficiency (B cell deficiency)
Immunodeficiency (defects in humoral immune responses)
Lymphoid Tissue Defects

Internal/Organ Research
Lymphoid Tissue Defects
Spleen Defects

Research Tools
Cancer Research (B cell deficiency)
Cancer Research (production of B cells and antibodies)
Genetics Research (Mutagenesis and Transgenesis: transcriptional activation)
Immunology and Inflammation Research (B cell deficiency)
Immunology and Inflammation Research (production of B cells and antibodies)
Immunology and Inflammation Research (production of B cells, antibodies T cell lines, and hybridomas)

Genes & Alleles

Gene & Allele Information

Allele Symbol		Mirn150tm1Rsky
Allele Name		targeted mutation 1, Klaus Rajewsky
Allele Type		Targeted (knock-out)
Common Name(s)		MiR-150;
Mutation Made By		Klaus Rajewsky,   Immune Disease Institute (formerly CBRI)
Strain of Origin		C57BL/6
ES Cell Line Name		Bruce 4
ES Cell Line Strain		C57BL/6
Gene Symbol and Name		Mirn150, microRNA 150
Chromosome		7
Gene Common Name(s)		mmu-mir-150;
Molecular Note		A targeting vector was designed to insert an frt-flanked pGK-Neo cassette 468 bp upstream of the miR-150 stem loop, with one loxP site placed immediately upstream of the pGK-Neo cassette and the other loxP site placed 146 bp downstream of the miR-150 stem loop. To delete the loxP site-flanked 679 bp miR-150 coding genomic region and the frt-flanked pGK-Neo cassette, mice were then bred to cre-expressing mice (C57BL/6 congenic background, similar to Stock No. 006054). The resulting mice have a single loxP site replacing the miR-150 coding region. [MGI Ref ID J:127234]

Genotyping

Genotyping Information

Genotyping Protocols

Mirn150^tm1Rsky, SEP PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Xiao C; Calado DP; Galler G; Thai TH; Patterson HC; Wang J; Rajewsky N; Bender TP; Rajewsky K. 2007. MiR-150 controls B cell differentiation by targeting the transcription factor c-Myb. Cell 131(1):146-59. [PubMed: 17923094]  [MGI Ref ID J:127234]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, homozygous mice may be bred together.
Mating System	Heterozygote x Heterozygote         (Female x Male)
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

 

This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.

Estimated Available for Sale Date: 29-DEC-08

Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.

View All Strains Under Development and On Hold

Supply Details

Standard Supply	Under Development for Distribution Colony
Supply Notes	This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	000664 C57BL/6J
	100007 CB6F1/J	(approximate)
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

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