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| These mice express enhanced green fluorescent protein (EGFP) under the control of the olfactory receptor 160 (M72) promoter. Glomeruli associated with this marker are located primarily on the dorsal surface of the olfactory bulb. Sporadic fluorescence is observed throughout this zone. This strain may be useful in studies of neuronal connectivity and axonal development. | |||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
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Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation N10pN1 Donating Investigator Peter Mombaerts, Max Planck Institute of Biophysics Description
These mice express enhanced green fluorescent protein (EGFP) under the control of the olfactory receptor 160 (M72) promoter. Glomeruli associated with this marker are located primarily on the dorsal surface of the olfactory bulb. Sporadic fluorescence is observed throughout this zone. This strain may be useful in studies of neuronal connectivity and axonal development.Development
The Olfr160tm6Mom mutation was generated in 129P2/OlaHsd derived E14 ES cells and the loxP-flanked neomycin resistance sequence was excised by breeding with a mouse carrying an EIIa-cre transgene (Lasko et al., 1996, Proceedings of the National Academy of Sciences, v93 p5860), a transgene generated in FVB/N but having been backcrossed to C57BL/6 before this cross and having unknown pedigree prior to that. The Olfr160tm6Mom mutation was backcrossed onto C57BL/6J for more than 10 generations before this strain was sent from Dr. Mombaerts to The Jackson Laboratory, where it was cryopreserved.
Related Strains
Strains carrying Olfr160tm6Mom allele
006678 STOCK Olfr160tm6Mom/MomJ View Strains carrying Olfr160tm6Mom (1 strain)
Additional Web Information
Congenic Nomenclature
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Olfr160tm6Mom relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Sensorineural Research
Research Tools
Sensorineural Research
Sensorineural Research
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Olfr160tm6Mom | ||
|---|---|---|---|
| Allele Name | targeted mutation 6, Peter Mombaerts | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | M72-GFP; M72-IRES-tauGFP; | ||
| Mutation Made By | Peter Mombaerts, Max Planck Institute of Biophysics | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Site of Expression | The tauGFP fusion protein is expressed in olfactory sensory neurons that express the olfactory receptor Olfr160. | ||
| Gene Symbol and Name | Olfr160, olfactory receptor 160 | ||
| Chromosome | 9 | ||
| Gene Common Name(s) | GA_x6K02T2PVTD-31389446-31388517; M72; MOR171-3; | ||
| Molecular Note | An IRES-tauGFP was inserted downstream of the endogenous coding sequence and the floxed neo was excised via transient expression of cre yielding an insertional mutation that results in bicistronic expression of the endogenous gene and tauGFP. [MGI Ref ID J:73384] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Olfr160tm6Mom, SEP PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Feinstein P; Mombaerts P. 2004. A contextual model for axonal sorting into glomeruli in the mouse olfactory system. Cell 117(6):817-31. [PubMed: 15186781] [MGI Ref ID J:90716]
Potter SM; Zheng C; Koos DS; Feinstein P; Fraser SE; Mombaerts P. 2001. Structure and emergence of specific olfactory glomeruli in the mouse. J Neurosci 21(24):9713-23. [PubMed: 11739580] [MGI Ref ID J:73384]
Additional References
Olfr160tm6Mom relatedHasegawa S; Hamada S; Kumode Y; Esumi S; Katori S; Fukuda E; Uchiyama Y; Hirabayashi T; Mombaerts P; Yagi T. 2008. The protocadherin-alpha family is involved in axonal coalescence of olfactory sensory neurons into glomeruli of the olfactory bulb in mouse. Mol Cell Neurosci 38(1):66-79. [PubMed: 18353676] [MGI Ref ID J:136920]
Health & husbandry
Health & Colony Maintenance Information
Currently there no information available for this strain. This may be due to the supply level of this strain.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00
Additional Supply Details
| Pricing for International shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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General Terms and Conditions
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