Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation N10pN1   Donating Investigator Peter Mombaerts,   Max Planck Institute of Biophysics

Development
The homologous recombination that inserted the IRES-taulacZ-LTNL cassette downstream of the endogenous coding sequence was done in D3 ES cells, which were derived from 129S2/SvPas. The loxP-flanked neo cassette was then removed by transient transfection of a plasmid expressing cre recombinase, prior to injection of these ES cells into blastocysts. Resulting mice were bred to C57BL/6 and intercrossed prior to backcrossing more than 10 generations to C57BL/6J.

Related Strains

Strains carrying   Olfr17^tm1Mom allele

006595

B6;129P2-Olfr17tm1Mom/MomJ

View Strains carrying   Olfr17^tm1Mom     (1 strain)

Strains carrying other alleles of Olfr17

006649	B6;129P2-Olfr17tm5Mom/MomJ
006650	STOCK Olfr17tm6Mom/MomJ
006669	STOCK Olfr17tm7Mom/MomJ
006651	STOCK-Olfr17tm4Mom/MomJ

View Strains carrying other alleles of Olfr17     (4 strains)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Olfr17^tm1Mom/Olfr17^tm1Mom

        involves: 129P2/OlaHsd * C57BL/6

• nervous system phenotype
• abnormal sensory neuron projections (MGI Ref ID J:90716)
  • axons coalesce into separate glomeruli

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Olfr17^tm1Mom related

Research Tools
Sensorineural Research

Sensorineural Research

Genes & Alleles

Gene & Allele Information

Allele Symbol		Olfr17tm1Mom
Allele Name		targeted mutation 1, Peter Mombaerts
Allele Type		Targeted (Reporter)
Common Name(s)		OR-lacZ; P2*-LacZ; P2-IRES-taulacZ;
Mutation Made By		Peter Mombaerts,   Max Planck Institute of Biophysics
Strain of Origin		129P2/OlaHsd
ES Cell Line Name		E14
ES Cell Line Strain		129P2/OlaHsd
Gene Symbol and Name		Olfr17, olfactory receptor 17
Chromosome		7
Gene Common Name(s)		GA_x6K02T2PBJ9-9470146-9471093; JCG5; MOR263-5; OR10A4P; P2; ratchr1-164484422-164485369_ORF;
Molecular Note		Insertion of an IRES-tau-lacZ-loxP-tk-neo-loxP expression cassette placed the inserted sequences under control of the endogenous regulatory sequences. Transient expression of Cre recombinase in C57BL/6 blastocysts excised the thymidine kinase and neomycin sequences. This allele expresses a bicistronic mRNA encoding the receptor and a bovine tau-beta-galactosidase fusion protein. [MGI Ref ID J:36595] [MGI Ref ID J:99181]

Genotyping

Genotyping Information

Genotyping Protocols

Olfr17^tm1Mom, SEP PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Feinstein P; Mombaerts P. 2004. A contextual model for axonal sorting into glomeruli in the mouse olfactory system. Cell 117(6):817-31. [PubMed: 15186781]  [MGI Ref ID J:90716]

Mombaerts P; Wang F; Dulac C; Chao SK; Nemes A; Mendelsohn M; Edmondson J; Axel R. 1996. Visualizing an olfactory sensory map. Cell 87(4):675-86. [PubMed: 8929536]  [MGI Ref ID J:36595]

Additional References

Olfr17^tm1Mom related

Biju KC; Marks DR; Mast TG; Fadool DA. 2008. Deletion of voltage-gated channel affects glomerular refinement and odorant receptor expression in the mouse olfactory system. J Comp Neurol 506(2):161-79. [PubMed: 18022950]  [MGI Ref ID J:132307]

Bulfone A; Wang F; Hevner R; Anderson S; Cutforth T; Chen S; Meneses J; Pedersen R; Axel R; Rubenstein JL. 1998. An olfactory sensory map develops in the absence of normal projection neurons or GABAergic interneurons. Neuron 21(6):1273-82. [PubMed: 9883721]  [MGI Ref ID J:51888]

Col JA; Matsuo T; Storm DR; Rodriguez I. 2007. Adenylyl cyclase-dependent axonal targeting in the olfactory system. Development 134(13):2481-9. [PubMed: 17537788]  [MGI Ref ID J:122528]

Costanzo RM; Perrino LA. 2008. Peak in matrix metaloproteinases-2 levels observed during recovery from olfactory nerve injury. Neuroreport 19(3):327-31. [PubMed: 18303576]  [MGI Ref ID J:133918]

Dudanova I; Tabuchi K; Rohlmann A; Sudhof TC; Missler M. 2007. Deletion of alpha-neurexins does not cause a major impairment of axonal pathfinding or synapse formation. J Comp Neurol 502(2):261-74. [PubMed: 17347997]  [MGI Ref ID J:132844]

Henion TR; Raitcheva D; Grosholz R; Biellmann F; Skarnes WC; Hennet T; Schwarting GA. 2005. Beta1,3-N-acetylglucosaminyltransferase 1 glycosylation is required for axon pathfinding by olfactory sensory neurons. J Neurosci 25(8):1894-903. [PubMed: 15728829]  [MGI Ref ID J:97372]

Laub F; Lei L; Sumiyoshi H; Kajimura D; Dragomir C; Smaldone S; Puche AC; Petros TJ; Mason C; Parada LF; Ramirez F. 2005. Transcription Factor KLF7 Is Important for Neuronal Morphogenesis in Selected Regions of the Nervous System. Mol Cell Biol 25(13):5699-711. [PubMed: 15964824]  [MGI Ref ID J:99181]

Schwarting GA; Raitcheva D; Crandall JE; Burkhardt C; Puschel AW. 2004. Semaphorin 3A-mediated axon guidance regulates convergence and targeting of P2 odorant receptor axons. Eur J Neurosci 19(7):1800-10. [PubMed: 15078553]  [MGI Ref ID J:89626]

Scolnick JA; Cui K; Duggan CD; Xuan S; Yuan XB; Efstratiadis A; Ngai J. 2008. Role of IGF signaling in olfactory sensory map formation and axon guidance. Neuron 57(6):847-57. [PubMed: 18367086]  [MGI Ref ID J:136049]

Shykind BM; Rohani SC; O'Donnell S; Nemes A; Mendelsohn M; Sun Y; Axel R; Barnea G. 2004. Gene switching and the stability of odorant receptor gene choice. Cell 117(6):801-15. [PubMed: 15186780]  [MGI Ref ID J:90717]

St John JA; Clarris HJ; McKeown S; Royal S; Key B. 2003. Sorting and convergence of primary olfactory axons are independent of the olfactory bulb. J Comp Neurol 464(2):131-40. [PubMed: 12898607]  [MGI Ref ID J:85051]

Taniguchi M; Nagao H; Takahashi YK; Yamaguchi M; Mitsui S; Yagi T; Mori K; Shimizu T. 2003. Distorted odor maps in the olfactory bulb of semaphorin 3A-deficient mice. J Neurosci 23(4):1390-7. [PubMed: 12598627]  [MGI Ref ID J:97369]

Zou DJ; Chesler AT; Le Pichon CE; Kuznetsov A; Pei X; Hwang EL; Firestein S. 2007. Absence of adenylyl cyclase 3 perturbs peripheral olfactory projections in mice. J Neurosci 27(25):6675-83. [PubMed: 17581954]  [MGI Ref ID J:122798]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

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In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.