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| These Thy1-GFPM transgenic mice may be useful in neurobiological studies for fluorescent labeling of neural tissues, especially for mossy fibers in the cerebellum and intense, yet sparse, labeling of a variety of neuronal subsets. Of note, this strain is one of many from the donating investigator with specific/differential fluorescent protein expression in neural tissues (see Stock No. 003709, Stock No. 003710, Stock No. 003782, and Stock No. 005630) or multiple fluorescent protein expression in neural tissues (see the Brainbow mice; Stock No. 007901, Stock No. 007910, Stock No. 007911, Stock No. 007921). | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Former Names B6.Cg-Tg(Thy1-EGFP)MJrs/J (Changed: 23-JUL-09 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Noncarrier x Hemizygote (Female x Male) 30-SEP-09 Species laboratory mouse Generation N10+1F3 (04-JAN-11)
Generation DefinitionsDonating Investigator Joshua R Sanes, Harvard University Description
Mice harboring the Thy1-GFP transgene are viable and fertile with enhanced green fluorescent protein (EGFP) expression under the control of a modified Thy1 promoter region (containing the sequences required for neuronal expression but lacking the sequences required for expression in non-neural cells). Homozygous or hemizygous Thy1-GFPM mice (derived from founder line M) express EGFP in sparse subsets of neurons within specific populations; providing a bright, vital Golgi-like stain. Less than 10% of all motor axons, retinal ganglion cells, lumbar dorsal root ganglion, and cortex express EGFP. These Thy1-GFPM transgenic mice may be useful in neurobiological studies for fluorescent labeling of neural tissues, especially for mossy fibers in the cerebellum and intense, yet sparse, labeling of a variety of neuronal subsets.This strain is one of many from the donating investigator with specific/differential fluorescent protein expression in neural tissues (see Stock No. 003709, Stock No. 003710, Stock No. 003782, and Stock No. 005630) or multiple fluorescent protein expression in neural tissues (see the Brainbow mice; Stock No. 007901, Stock No. 007910, Stock No. 007911, Stock No. 007921).
Development
The Thy1-GFP transgene was designed with a modified regulatory region of the "murine thy1.2 gene" (extending from the promoter to the intron following exon 4, excluding exon 3 and its flanking introns) upstream of an enhanced green fluorescent protein (EGFP) sequence. This transgene was injected into (C57BL/6J and CBA)F1 fertilized oocytes. Transgenic founders (line M) were bred with C57BL/6J to generate Thy1-GFPM mice. The donating investigator reports Thy1-GFPM transgenic mice were then backcrossed to C57BL6/J mice for at least 10 generations. Breeding difficulties (perhaps due to the amount of inbreeding to C57BL/6) caused the donating investigator to "out-breed" the strain to unspecified mice for at least one generation prior to sending to The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J for at least one generation to establish the colony.A 27 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory on the rederived mice revealed that this strain is on a mixed background (six markers are segregating: 4 as CBA and 2 as neither C57BL/6 nor CBA) (July 2009).
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (357 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Cancer Research
xenograft/transplant host
Developmental Biology Research
transplantation marker for embryonic and adult tissue
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: astrocytes, neurons
Tissue/Cell Markers: multiple
Tissue/Cell Markers: neurons
Tissue/Cell Markers: transplantation marker for embryonic and adult tissue
Neurobiology Research
cell marker
Toxicology Research
xenograft/transplant host
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Thy1-EGFP)MJrs | ||
|---|---|---|---|
| Allele Name | transgene insertion M, Joshua R Sanes | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | GFP line-M; GFP-M; Thy1-GFP line M; Thy1-GFP/M; Thy1-GFPM; Thy1::GFP-M; | ||
| Mutation Made By | Joshua Sanes, Harvard University | ||
| Strain of Origin | (C57BL/6J x CBA)F1 | ||
| Site of Expression | mossy fibers in the cerebellum and intense, yet sparse, labeling of a variety of neuronal subsets | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory | ||
| Molecular Note | The Thy1-GFP transgene was designed with a modified regulatory region of the "murine thy1.2 gene" (extending from the promoter to the intron following exon 4, excluding exon 3 and its flanking introns) upstream of an enhanced green fluorescent protein (EGFP) sequence. Line M was subsequently established and analyzed. Expression of EGFP is detected primarily in mossy fibers in internal granule layer of the cerebellum (10-80% incidence). Less than 10% of all motor axons, retinal ganglion cells, lumbar dorsal root ganglion, and cortex express EGFP, with no reported expression in other tested neuronal tissues. [MGI Ref ID J:93699] | ||
| Gene Symbol and Name | Tg(Thy1-EGFP)MJrs, transgene insertion M, Joshua R Sanes | ||
| Chromosome | UN | ||
| Gene Common Name(s) | GFP line-M; GFP-M; Thy1-GFP line M; Thy1-GFPM; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Thy1-YFP)HJrs-alternate2,MELT
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Feng G; Mellor RH; Bernstein M; Keller-Peck C; Nguyen QT; Wallace M; Nerbonne JM; Lichtman JW; Sanes JR. 2000. Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron 28(1):41-51. [PubMed: 11086982] [MGI Ref ID J:93699]
Additional References
Tg(Thy1-EGFP)MJrs relatedAllen NJ; Bennett ML; Foo LC; Wang GX; Chakraborty C; Smith SJ; Barres BA. 2012. Astrocyte glypicans 4 and 6 promote formation of excitatory synapses via GluA1 AMPA receptors. Nature 486(7403):410-4. [PubMed: 22722203] [MGI Ref ID J:186711]
Belichenko NP; Belichenko PV; Mobley WC. 2009. Evidence for both neuronal cell autonomous and nonautonomous effects of methyl-CpG-binding protein 2 in the cerebral cortex of female mice with Mecp2 mutation. Neurobiol Dis 34(1):71-7. [PubMed: 19167498] [MGI Ref ID J:147302]
Carlisle HJ; Luong TN; Medina-Marino A; Schenker L; Khorosheva E; Indersmitten T; Gunapala KM; Steele AD; O'Dell TJ; Patterson PH; Kennedy MB. 2011. Deletion of Densin-180 Results in Abnormal Behaviors Associated with Mental Illness and Reduces mGluR5 and DISC1 in the Postsynaptic Density Fraction. J Neurosci 31(45):16194-207. [PubMed: 22072671] [MGI Ref ID J:177836]
Carlisle HJ; Manzerra P; Marcora E; Kennedy MB. 2008. SynGAP regulates steady-state and activity-dependent phosphorylation of cofilin. J Neurosci 28(50):13673-83. [PubMed: 19074040] [MGI Ref ID J:143044]
Chow DK; Groszer M; Pribadi M; Machniki M; Carmichael ST; Liu X; Trachtenberg JT. 2009. Laminar and compartmental regulation of dendritic growth in mature cortex. Nat Neurosci 12(2):116-8. [PubMed: 19151711] [MGI Ref ID J:145970]
Clement JP; Aceti M; Creson TK; Ozkan ED; Shi Y; Reish NJ; Almonte AG; Miller BH; Wiltgen BJ; Miller CA; Xu X; Rumbaugh G. 2012. Pathogenic SYNGAP1 mutations impair cognitive development by disrupting maturation of dendritic spine synapses. Cell 151(4):709-23. [PubMed: 23141534] [MGI Ref ID J:193208]
Filosa A; Paixao S; Honsek SD; Carmona MA; Becker L; Feddersen B; Gaitanos L; Rudhard Y; Schoepfer R; Klopstock T; Kullander K; Rose CR; Pasquale EB; Klein R. 2009. Neuron-glia communication via EphA4/ephrin-A3 modulates LTP through glial glutamate transport. Nat Neurosci 12(10):1285-92. [PubMed: 19734893] [MGI Ref ID J:154648]
King AE; Blizzard CA; Southam KA; Vickers JC; Dickson TC. 2012. Degeneration of axons in spinal white matter in G93A mSOD1 mouse characterized by NFL and alpha-internexin immunoreactivity. Brain Res 1465:90-100. [PubMed: 22609817] [MGI Ref ID J:186439]
Kvajo M; McKellar H; Drew LJ; Lepagnol-Bestel AM; Xiao L; Levy RJ; Blazeski R; Arguello PA; Lacefield CO; Mason CA; Simonneau M; O'Donnell JM; MacDermott AB; Karayiorgou M; Gogos JA. 2011. Altered axonal targeting and short-term plasticity in the hippocampus of Disc1 mutant mice. Proc Natl Acad Sci U S A 108(49):E1349-58. [PubMed: 22049344] [MGI Ref ID J:180193]
Li M; Masugi-Tokita M; Takanami K; Yamada S; Kawata M. 2012. Testosterone has sublayer-specific effects on dendritic spine maturation mediated by BDNF and PSD-95 in pyramidal neurons in the hippocampus CA1 area. Brain Res 1484:76-84. [PubMed: 23010313] [MGI Ref ID J:193568]
Lopez-Atalaya JP; Ciccarelli A; Viosca J; Valor LM; Jimenez-Minchan M; Canals S; Giustetto M; Barco A. 2011. CBP is required for environmental enrichment-induced neurogenesis and cognitive enhancement. EMBO J 30(20):4287-98. [PubMed: 21847097] [MGI Ref ID J:177196]
Matsuoka RL; Chivatakarn O; Badea TC; Samuels IS; Cahill H; Katayama K; Kumar SR; Suto F; Chedotal A; Peachey NS; Nathans J; Yoshida Y; Giger RJ; Kolodkin AL. 2011. Class 5 transmembrane semaphorins control selective Mammalian retinal lamination and function. Neuron 71(3):460-73. [PubMed: 21835343] [MGI Ref ID J:174679]
Matter C; Pribadi M; Liu X; Trachtenberg JT. 2009. Delta-catenin is required for the maintenance of neural structure and function in mature cortex in vivo. Neuron 64(3):320-7. [PubMed: 19914181] [MGI Ref ID J:155761]
Mazzoni F; Novelli E; Strettoi E. 2008. Retinal ganglion cells survive and maintain normal dendritic morphology in a mouse model of inherited photoreceptor degeneration. J Neurosci 28(52):14282-92. [PubMed: 19109509] [MGI Ref ID J:143877]
Mishra A; Knerr B; Paixao S; Kramer ER; Klein R. 2008. The protein dendrite arborization and synapse maturation 1 (Dasm-1) is dispensable for dendrite arborization. Mol Cell Biol 28(8):2782-91. [PubMed: 18268009] [MGI Ref ID J:133898]
Paolicelli RC; Bolasco G; Pagani F; Maggi L; Scianni M; Panzanelli P; Giustetto M; Ferreira TA; Guiducci E; Dumas L; Ragozzino D; Gross CT. 2011. Synaptic pruning by microglia is necessary for normal brain development. Science 333(6048):1456-8. [PubMed: 21778362] [MGI Ref ID J:175794]
Risher WC; Andrew RD; Kirov SA. 2009. Real-time passive volume responses of astrocytes to acute osmotic and ischemic stress in cortical slices and in vivo revealed by two-photon microscopy. Glia 57(2):207-21. [PubMed: 18720409] [MGI Ref ID J:156224]
Rust MB; Gurniak CB; Renner M; Vara H; Morando L; Gorlich A; Sassoe-Pognetto M; Banchaabouchi MA; Giustetto M; Triller A; Choquet D; Witke W. 2010. Learning, AMPA receptor mobility and synaptic plasticity depend on n-cofilin-mediated actin dynamics. EMBO J :. [PubMed: 20407421] [MGI Ref ID J:159455]
Sakai Y; Tanaka T; Seki M; Okuyama S; Fukuchi T; Yamagata K; Takei N; Nawa H; Abe H. 2009. Cyclooxygenase-2 plays a critical role in retinal ganglion cell death after transient ischemia: real-time monitoring of RGC survival using Thy-1-EGFP transgenic mice. Neurosci Res 65(4):319-25. [PubMed: 19698752] [MGI Ref ID J:157323]
Senturk A; Pfennig S; Weiss A; Burk K; Acker-Palmer A. 2011. Ephrin Bs are essential components of the Reelin pathway to regulate neuronal migration. Nature 472(7343):356-60. [PubMed: 21460838] [MGI Ref ID J:171375]
Stark KL; Xu B; Bagchi A; Lai WS; Liu H; Hsu R; Wan X; Pavlidis P; Mills AA; Karayiorgou M; Gogos JA. 2008. Altered brain microRNA biogenesis contributes to phenotypic deficits in a 22q11-deletion mouse model. Nat Genet 40(6):751-60. [PubMed: 18469815] [MGI Ref ID J:136969]
Stritt C; Knoll B. 2010. Serum response factor regulates hippocampal lamination and dendrite development and is connected with reelin signaling. Mol Cell Biol 30(7):1828-37. [PubMed: 20123976] [MGI Ref ID J:161724]
Wilbrecht L; Holtmaat A; Wright N; Fox K; Svoboda K. 2010. Structural plasticity underlies experience-dependent functional plasticity of cortical circuits. J Neurosci 30(14):4927-32. [PubMed: 20371813] [MGI Ref ID J:165065]
Wills ZP; Mandel-Brehm C; Mardinly AR; McCord AE; Giger RJ; Greenberg ME. 2012. The nogo receptor family restricts synapse number in the developing hippocampus. Neuron 73(3):466-81. [PubMed: 22325200] [MGI Ref ID J:182292]
Wu JI; Lessard J; Olave IA; Qiu Z; Ghosh A; Graef IA; Crabtree GR. 2007. Regulation of dendritic development by neuron-specific chromatin remodeling complexes. Neuron 56(1):94-108. [PubMed: 17920018] [MGI Ref ID J:132101]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number MGL377
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier) siblings. The donating investigator suggests that backcrossing these mice onto the C57BL/6 genetic background may lead to breeding difficulties. Mating System Noncarrier x Hemizygote (Female x Male) 30-SEP-09 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(Thy1-EGFP)MJrs
Price per Pair (US dollars $) Pair Genotype $296.00 Hemizygous for Tg(Thy1-EGFP)MJrs x Noncarrier $296.00 Noncarrier x Hemizygous for Tg(Thy1-EGFP)MJrs Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(Thy1-EGFP)MJrs
Price per Pair (US dollars $) Pair Genotype $384.80 Hemizygous for Tg(Thy1-EGFP)MJrs x Noncarrier $384.80 Noncarrier x Hemizygous for Tg(Thy1-EGFP)MJrs Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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|
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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