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| These Thy1-GFPM transgenic mice may be useful in neurobiological studies for fluorescent labeling of neural tissues, especially for mossy fibers in the cerebellum and intense, yet sparse, labeling of a variety of neuronal subsets. Of note, this strain is one of many from the donating investigator with specific/differential fluorescent protein expression in neural tissues (see Stock No. 003709, Stock No. 003710, Stock No. 003782, and Stock No. 005630) or multiple fluorescent protein expression in neural tissues (see the Brainbow mice; Stock No. 007901, Stock No. 007910, Stock No. 007911, Stock No. 007921). | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
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Description
Strain Information
Former Names B6.Cg-Tg(Thy1-EGFP)MJrs/J (Changed: 23-JUL-09 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Noncarrier x Hemizygote (Female x Male) 30-SEP-09 Species laboratory mouse Generation F1+ (22-JUL-09) Donating Investigator Joshua Sanes, Harvard University Description
Mice harboring the Thy1-GFP transgene are viable and fertile with enhanced green fluorescent protein (EGFP) expression under the control of a modified Thy1 promoter region (containing the sequences required for neuronal expression but lacking the sequences required for expression in non-neural cells). Homozygous or hemizygous Thy1-GFPM mice (derived from founder line M) express EGFP in sparse subsets of neurons within specific populations; providing a bright, vital Golgi-like stain. Less than 10% of all motor axons, retinal ganglion cells, lumbar dorsal root ganglion, and cortex express EGFP. These Thy1-GFPM transgenic mice may be useful in neurobiological studies for fluorescent labeling of neural tissues, especially for mossy fibers in the cerebellum and intense, yet sparse, labeling of a variety of neuronal subsets.This strain is one of many from the donating investigator with specific/differential fluorescent protein expression in neural tissues (see Stock No. 003709, Stock No. 003710, Stock No. 003782, and Stock No. 005630) or multiple fluorescent protein expression in neural tissues (see the Brainbow mice; Stock No. 007901, Stock No. 007910, Stock No. 007911, Stock No. 007921).
Development
The Thy1-GFP transgene was designed with a modified regulatory region of the "murine thy1.2 gene" (extending from the promoter to the intron following exon 4, excluding exon 3 and its flanking introns) upstream of an enhanced green fluorescent protein (EGFP) sequence. This transgene was injected into (C57BL/6J and CBA)F1 fertilized oocytes. Transgenic founders (line M) were bred with C57BL/6J to generate Thy1-GFPM mice. The donating investigator reports Thy1-GFPM transgenic mice were then backcrossed to C57BL6/J mice for at least 10 generations. Breeding difficulties (perhaps due to the amount of inbreeding to C57BL/6) caused the donating investigator to "out-breed" the strain to unspecified mice for at least one generation prior to sending to The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J for at least one generation to establish the colony. A 27 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory on the rederived mice revealed that this strain is on a mixed background (six markers are segregating: 4 as CBA and 2 as neither C57BL/6 nor CBA) (July 2009).
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Cancer Research
xenograft/transplant host
Developmental Biology Research
transplantation marker for embryonic and adult tissue
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers: astrocytes, neurons
Tissue/Cell Markers: multiple
Tissue/Cell Markers: neurons
Tissue/Cell Markers: transplantation marker for embryonic and adult tissue
Neurobiology Research
cell marker
Toxicology Research
xenograft/transplant host
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Thy1-EGFP)MJrs | ||
|---|---|---|---|
| Allele Name | transene insertion M, Joshua R Sanes | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | GFP line-M; GFP-M; Thy1-GFP line M; Thy1-GFPM; | ||
| Mutation Made By | Joshua Sanes, Harvard University | ||
| Strain of Origin | (C57BL/6J x CBA)F1 | ||
| Site of Expression | mossy fibers in the cerebellum and intense, yet sparse, labeling of a variety of neuronal subsets | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory | ||
| Molecular Note | The Thy1-GFP transgene was designed with a modified regulatory region of the "murine thy1.2 gene" (extending from the promoter to the intron following exon 4, excluding exon 3 and its flanking introns) upstream of an enhanced green fluorescent protein (EGFP) sequence. Line M was subsequently established and analyzed. Expression of EGFP is detected primarily in mossy fibers in internal granule layer of the cerebellum (10-80% incidence). Less than 10% of all motor axons, retinal ganglion cells, lumbar dorsal root ganglion, and cortex express EGFP, with no reported expression in other tested neuronal tissues. [MGI Ref ID J:93699] | ||
| Gene Symbol and Name | Tg(Thy1-EGFP)MJrs, transene insertion M, Joshua R Sanes | ||
| Chromosome | UN | ||
| Gene Common Name(s) | GFP line-M; GFP-M; Thy1-GFP line M; Thy1-GFPM; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Melt Curve Analysis
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Feng G; Mellor RH; Bernstein M; Keller-Peck C; Nguyen QT; Wallace M; Nerbonne JM; Lichtman JW; Sanes JR. 2000. Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron 28(1):41-51. [PubMed: 11086982] [MGI Ref ID J:93699]
Additional References
Tg(Thy1-EGFP)MJrs relatedBelichenko NP; Belichenko PV; Mobley WC. 2009. Evidence for both neuronal cell autonomous and nonautonomous effects of methyl-CpG-binding protein 2 in the cerebral cortex of female mice with Mecp2 mutation. Neurobiol Dis 34(1):71-7. [PubMed: 19167498] [MGI Ref ID J:147302]
Carlisle HJ; Manzerra P; Marcora E; Kennedy MB. 2008. SynGAP regulates steady-state and activity-dependent phosphorylation of cofilin. J Neurosci 28(50):13673-83. [PubMed: 19074040] [MGI Ref ID J:143044]
Chow DK; Groszer M; Pribadi M; Machniki M; Carmichael ST; Liu X; Trachtenberg JT. 2009. Laminar and compartmental regulation of dendritic growth in mature cortex. Nat Neurosci 12(2):116-8. [PubMed: 19151711] [MGI Ref ID J:145970]
Mazzoni F; Novelli E; Strettoi E. 2008. Retinal ganglion cells survive and maintain normal dendritic morphology in a mouse model of inherited photoreceptor degeneration. J Neurosci 28(52):14282-92. [PubMed: 19109509] [MGI Ref ID J:143877]
Mishra A; Knerr B; Paixao S; Kramer ER; Klein R. 2008. The protein dendrite arborization and synapse maturation 1 (Dasm-1) is dispensable for dendrite arborization. Mol Cell Biol 28(8):2782-91. [PubMed: 18268009] [MGI Ref ID J:133898]
Stark KL; Xu B; Bagchi A; Lai WS; Liu H; Hsu R; Wan X; Pavlidis P; Mills AA; Karayiorgou M; Gogos JA. 2008. Altered brain microRNA biogenesis contributes to phenotypic deficits in a 22q11-deletion mouse model. Nat Genet 40(6):751-60. [PubMed: 18469815] [MGI Ref ID J:136969]
Wu JI; Lessard J; Olave IA; Qiu Z; Ghosh A; Graef IA; Crabtree GR. 2007. Regulation of dendritic development by neuron-specific chromatin remodeling complexes. Neuron 56(1):94-108. [PubMed: 17920018] [MGI Ref ID J:132101]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number MGL375/MGL377
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier) siblings. The donating investigator suggests that backcrossing these mice onto the C57BL/6 genetic background may lead to breeding difficulties. Mating System Noncarrier x Hemizygote (Female x Male) 30-SEP-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(Thy1-EGFP)MJrs
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(Thy1-EGFP)MJrs x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(Thy1-EGFP)MJrs
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(Thy1-EGFP)MJrs
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(Thy1-EGFP)MJrs x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(Thy1-EGFP)MJrs
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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