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| Deletion of an anti-silencing element (ASE) located between the Rag1 and Rag2 genes unmasks an intergenic silencer that abrogates Rag gene expression in developing CD4+CD8+ T cells. | |||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Donating Investigator Michel Nussenzweig, The Rockefeller University Description
Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. Deletion of an anti-silencing element (ASE) located between the Rag1 and Rag2 genes unmasks an intergenic silencer that abrogates Rag gene expression in developing CD4+CD8+ T cells. Rag1 and Rag2 transcript levels decrease to almost below the detection limit, as measured by RT-PCR. Expression is decreased to a lesser extent in CD4-CD8- thymocytes. Small decreases in mRNA abundance in pro-B and pre-B cells are within the margin of error for the experiments.Development
A targeting vector was designed to remove a 7.6 kb genomic segment encoding the antisilencing element (ASE) between the Rag1 and Rag2 genes and replace it with a loxP-flanked neomycin-Cre self excision cassette. Cre was driven by the angiotensin converting enzyme promoter, specific for testis expression. The vector was introduced to E14 129P2/OlaHsd-derived embryonic stem (ES) cells. Excision left a single loxP site in place of the deleted element. This strain has been backcrossed two times by the donating laboratory.
Related Strains
Strains carrying other alleles of Rag1
View Strains carrying other alleles of Rag1 (18 strains)
008309 C57BL/6-Rag2tm1Cgn/J 005688 FVB-Tg(Rag2-EGFP)1Mnz/J
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Immunology and Inflammation Research
Immunodeficiency (B and T cell deficiency)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Rag1/Rag2tm1Mnz | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Michel C Nussenzweig | ||
| Allele Type | Targeted (other) | ||
| Common Name(s) | ASE ko; | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Gene Symbol and Name | Rag1, recombination activating gene 1 | ||
| Chromosome | 2 | ||
| Gene Common Name(s) | MGC43321; RNF74; Rag-1; | ||
| Molecular Note | A targeting vector was designed to remove a 7.6 kb genomic segment encoding the antisilencing element (ASE) between the Rag1 and Rag2 genes and replace it with a loxP-flanked neomycin-Cre self excision cassette. Cre was driven by the angiotensin converting enzyme promoter, specific for testis expression. Excision left a single loxP site in place of the deleted element. [MGI Ref ID J:90847] | ||
| Gene Symbol and Name | Rag2, recombination activating gene 2 | ||
| Chromosome | 2 | ||
| Gene Common Name(s) | RAG-2; Rag-2; | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Yannoutsos N; Barreto V; Misulovin Z; Gazumyan A; Yu W; Rajewsky N; Peixoto BR; Eisenreich T; Nussenzweig MC. 2004. A cis element in the recombination activating gene locus regulates gene expression by counteracting a distant silencer. Nat Immunol 5(4):443-50. [PubMed: 15021880] [MGI Ref ID J:90487]
Additional References
Rag1/Rag2tm1Mnz relatedHirota J; Mombaerts P. 2004. The LIM-homeodomain protein Lhx2 is required for complete development of mouse olfactory sensory neurons. Proc Natl Acad Sci U S A 101(23):8751-5. [PubMed: 15173589] [MGI Ref ID J:90847]
Health & husbandry
Health & Colony Maintenance Information
Currently there no information available for this strain. This may be due to the supply level of this strain.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00
Additional Supply Details
| Pricing for International shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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General Terms and Conditions
See Terms of Use
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