Strain Name: |
C.129S(B6)-Stat5atm1Mam/J |
|---|---|
Stock Number: |
007806 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Stat5a; Stat5atm1Mam; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Heterozygote x Heterozygote (Female x Male) Species laboratory mouse Donating Investigator Michael Tomasson, Washington University School of Medicine Generation N5+ (05-AUG-08) Strain Description
Mice homozygous for the targeted mutation are viable, develop normally, and are fertile, but females do not lactate. Mammary ductal development through pregnancy is normal, but lobuloalveolar development is severely reduced, and milk is not secreted even after prolonged suckling. Levels of the closely-related STAT5B protein are also markedly reduced in STAT5A-deficient mice, but STAT5B protein levels increase and phosphorylation is evident after 3 days of suckling. Expression of several milk protein genes is unaffected in homozygous mutants, but whey acidic protein expression is severely reduced. Homozygous males exhibit abnormal prostate morphology and an increase in probasin secretion. Immmunological defects exhibited by homozygotes include a reduced number of peritoneal mast cells, decreased IL-3-dependent development of bone marrow-derived mast cells, defective bone marrow-derived macrophage GM-CSF (Granulocyte-Macrophage Colony-Stimulating Factor)-induced proliferation, decreased number of NK cells, and decreased expansion of T cells. This mutant mouse strain may be useful in studies of mammary gland development, lactogenesis, hematopoiesis, and immunological intracellular signal transduction.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Strain Development
A targeting vector containing phosphoglycerate kinase promoter-driven neomycin resistance gene casette and a herpes simplex virus thymidine kinase gene was used to disrupt a region containing a non-coding exon, 2 coding exons and flanking sequence. The construct was electroporated into 129S6/SvEvTac derived TC-1 embryonic stem (ES) cells, and correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to 129 mice, and then backcrossed to C57BL/6 for one generation. The donating investigator backcrossed the strain to BALB/cAnNTac for 3 generations using a marker-assisted protocol. The mice were subsequently backcrossed to BALB/cJ for a single generation.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Stat5atm1Mam | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Lothar Hennighausen | ||
| Common Name(s) | Stat5a-; | ||
| Mutation Made By | Lothar Hennighausen, National Institutes of Health | ||
| Strain of Origin | 129S6/SvEvTac | ||
| ES Cell Line Name | TC-1 | ||
| ES Cell Line Strain | 129S6/SvEvTac | ||
| Gene Symbol and Name | Stat5a, signal transducer and activator of transcription 5A | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | AA959963; MGF; STAT5; expressed sequence AA959963; | ||
| Molecular Note | The promoter sequence, an untranslated exon, and two coding exons were replaced by a neomycin selection cassette. Western blot analysis of extracts immunoprecipitated from mammary tissue of homozygous mutant mice showed an absence of encoded protein. [MGI Ref ID J:64278] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Stat5atm1Mam
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred heterozygous females x homozygous males. Although homozygous females are viable and fertile, they do not lactate. Litters from homozygous females must be fostered. |
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| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying Stat5atm1Mam allele
002833 B6;129S6-Stat5atm1Mam/J 003502 FVB.129S6-Stat5atm1Mam/J View Strains carrying Stat5atm1Mam (2 strains)
Additional Web Information
Congenic Nomenclature
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
Stat5atm1Mam relatedCancer Research
Genes Regulating Growth and Proliferation
Hematological Research
Mast Cell Deficiency
Immunology and Inflammation Research
Immunodeficiency (NK Cell Deficiency)
Immunodeficiency (T cell deficiency)
Intracellular Signaling Molecules
Cancer Research
Genes Regulating Growth and Proliferation
Immunology and Inflammation Research
Immunodeficiency (Mast Cell Deficiency)
Intracellular Signaling Molecules
Research Tools
Immunology and Inflammation Research (Mast Cell Deficiency)
References
Selected Reference(s)
Additional ReferencesLiu X; Robinson GW; Wagner KU; Garrett L; Wynshaw-Boris A; Hennighausen L. 1997. Stat5a is mandatory for adult mammary gland development and lactogenesis. Genes Dev 11(2):179-86. [PubMed: 9009201] [MGI Ref ID J:64278]
Price and Supply Information
| Strain Name: | C.129S(B6)-Stat5atm1Mam/J |
| Stock Number: | 007806 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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