Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N9+N1F2pN1 Generation Definitions   Donating Investigator Yasunori Hayashi,   Brain Science Institute, RIKEN

Description
Mice homozygous for this NR3B (Grin3b) mutant allele are viable and fertile, with no RNA from the targeted allele expressed in adult spinal cord. Homozygotes show a moderate but significant impairment in motor learning or coordination, decreased body weight with age, and decreased activity in their home cages. In addition, homozygous mice exhibit highly increased social interactions with their familiar cage mates in their home cage, with moderately increased anxiety-like behavior and decreased social interaction in novel environments. These NR3B (Grin3b) mutant mice may be useful in neurobiological studies; specifically studies involving NMDA receptor and NR3B function in cranial and spinal somatic motor neurons.

Development
A targeting vector was designed to replace exon 1 of the targeted gene with a loxP-flanked PGK-neo cassette. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. Chimeric males were first mated with females expressing Cre recombinase (C57BL/6 genetic background) to remove the selection cassette (leaving a single loxP site). The resulting mice were backcrossed to C57BL/6J for at least 8 generations prior to arrival at The Jackson Laboratory. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6J genetic background.

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Grin3b

016925

129;B6-Grin3b/Tmem259tm1Zhang Tg(Prnp-C19ORF6,-GFP)6Zhang/J

View Strains carrying other alleles of Grin3b     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Grin3b^tm1Yaha/Grin3b^tm1Yaha

        B6.129-Grin3b^tm1Yaha

• growth/size phenotype
• slow postnatal weight gain
  • decreased body weight becomes apparent at around 16-18 weeks of age and persists through 13 weeks   (MGI Ref ID J:126239)
  • food and water intake are normal   (MGI Ref ID J:126239)
• behavior/neurological phenotype
• abnormal motor capabilities/coordination/movement   (MGI Ref ID J:126239)
• • hypoactivity
    • reduced home cage activity   (MGI Ref ID J:126239)
    • generally hypoactive   (MGI Ref ID J:126239)
  • impaired coordination
    • mild impairment as indicated by an accelerating rotarod test   (MGI Ref ID J:126239)
    • no improvement with practice   (MGI Ref ID J:126239)
    • shorter distances walked in "beam" tests   (MGI Ref ID J:126239)
• abnormal social/conspecific interaction
  • increased social activity with familiar cagemates   (MGI Ref ID J:126239)
• • abnormal social investigation
    • reduced interactions with unknown animals when in a novel environment   (MGI Ref ID J:126239)
• increased anxiety-related response
  • reduced interactions with unknown animals when in a novel environment   (MGI Ref ID J:126239)
  • reduced stereotypic activity in a novel environment   (MGI Ref ID J:126239)
  • less frequent entry into the arms of an elevated plus maze, particularly the open arms   (MGI Ref ID J:126239)
  • less time spent in the arms of an elevated plus maze   (MGI Ref ID J:126239)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Behavioral and Learning Defects
Receptor Defects

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Grin3btm1Yaha
Allele Name		targeted mutation 1, Yasunori Hayashi
Allele Type		Targeted (knock-out)
Common Name(s)		NR3B-;
Mutation Made By		Yasunori Hayashi,   Brain Science Institute, RIKEN
Strain of Origin		129
ES Cell Line Strain		129
Gene Symbol and Name		Grin3b, glutamate receptor, ionotropic, NMDA3B
Chromosome		10
Gene Common Name(s)		GluN3B; NMDAR3B; NR3B;
Molecular Note		Exon 1, which contains the initiation codon and sequences encoding the signal peptide, was replaced with a loxP-flanked neomycin selection cassette. The selection cassette was removed through Cre-mediated recombination, leaving a single loxP site in place of exon 1. RT-PCR and in situ hybridization analysis confirmed that no detectable transcript is expressed from this allele. [MGI Ref ID J:126239]

Genotyping

Genotyping Information

Genotyping Protocols

Grin3b^tm1Yaha, Melt Curve Analysis
Grin3b^tm1Yaha, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Niemann S; Kanki H; Fukui Y; Takao K; Fukaya M; Hynynen MN; Churchill MJ; Shefner JM; Bronson RT; Brown RH Jr; Watanabe M; Miyakawa T; Itohara S; Hayashi Y. 2007. Genetic ablation of NMDA receptor subunit NR3B in mouse reveals motoneuronal and nonmotoneuronal phenotypes. Eur J Neurosci 26(6):1407-20. [PubMed: 17880385]  [MGI Ref ID J:126239]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, homozygous mice may be bred together.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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