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Genes & Alleles

Mc3r;   Mc3rtm1Cone;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Donating Investigator Roger Cone,   Vollum Institute Generation N7+F1 (08-MAY-08)

Strain Description
Mice that are homozygous for the targeted mutation are viable and fertile. Female homozygotes at approximately 50 to 60 days of age exhibit significantly heavier body weight when compared to heterozygotes. No gene product (mRNA) is detected by RT-PCR analysis of hypothalamus tissue. Male homozygotes exhibit an approximately 40% increase in body fat at 15-20 weeks of age when compared to wildtype. Heterozygotes display an intermediate increase in body fat percentage of approximately 36%. Although homozygotes have increased adiposity, there is no increased food intake or weight gain and resting basal or total oxygen consumption is lower in mutant mice when compared to wildtype. High fat diet causes an increased respiratory quotient within 24 hours of the diet change. Homozygotes on a low fat diet have a lower respiratory quotient than wildtype. Male homozygotes exhibit an approximately 50% decrease in wheel running behavior. Mutant mice become hypertensive with increased gamma-melanocyte-stimulating hormone plasma levels when placed on high sodium diet. Experimentally induced illness results in enhanced cachexia. This mutant mouse strain may be useful in studies of energy homeostasis, regulation of satiety, salt-sensitive hypertension and metabolism.

Strain Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt 0.7kb of encoding sequence including the initiation codon. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice, and then backcrossed to the same for more than 10 generations.

Mammalian Phenotype Terms assigned by genotype Mc3rtm1Cone/Mc3rtm1Cone         B6.129S4-Mc3rtm1Cone behavior/neurological phenotype decreased eating behavior (MGI Ref ID J:109494) after a 16-hour fast, i.p. injection of CCK-8 into females produces a 50% inhibition of food intake in the first 30 minutes in mutants and wild type and intake continued to be inhibited for up to 180 minutes The following phenotype information may relate to a genetic background differing from this JAX® Mice strain. Mc3rtm1Cone/Mc3r+         involves: 129S4/SvJae * C57BL/6J growth/size phenotype obese (MGI Ref ID J:78132) heterozygous mice show an intermediate increase in percentage of body fat compared to wild type (31.8%) and null mice (45.8%) around 15-20 weeks of age Mc3rtm1Cone/Mc3rtm1Cone         involves: 129S4/SvJae * C57BL/6J growth/size phenotype increased body weight (MGI Ref ID J:78132) female null mice are significantly heavier than wild type around 50-60 days of age obese (MGI Ref ID J:78132) mutants are obese with the proportion of fat increasing by ~40% around 15-20 weeks of age compared to wild type (31.8% in wild type vs 45.2% in Mc3r-null mice) homeostasis/metabolism phenotype abnormal respiratory quotient (MGI Ref ID J:78132) mice show a trend toward greater oxygen consumption on low or high fat diets compared to wild type when switched to high fat chow, mutants show a dramatic increase in respiratory quotient 24 hours after the switch mutants show a reduced RQ compared to wild type on a low fat chow diet and a higher RQ on high fat chow behavior/neurological phenotype abnormal food intake (MGI Ref ID J:78132) despite increased body fat, mice do not exhibit weight gain or increased food consumption adipose tissue phenotype increased adipose tissue amount (MGI Ref ID J:78132) on a low-fat chow, mutants show increased fat content without gross weight being different

Gene & Allele Details

Allele Symbol		Mc3rtm1Cone
Allele Name		targeted mutation 1, Roger D Cone
Common Name(s)		mc3r-/-;
Mutation Made By		Jonathan Murphy,   Oregon Health & Sciences University
Strain of Origin		129S4/SvJae
ES Cell Line Name		J1
ES Cell Line Strain		129S4/SvJae
Gene Symbol and Name		Mc3r, melanocortin 3 receptor
Chromosome		2
Gene Common Name(s)		MC3; MC3-R;
Molecular Note		0.7kb of sequence was deleted by the insertion of a neomycin selection cassette at the initiation codon. Homozygous mutant mice were shown to lack transcript via RT-PCR analysis of total hypthalamic RNA. [MGI Ref ID J:78132]

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Genotyping Protocols

Mc3r^tm1Cone

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as homozygotes.
Diet Information	LabDiet® 5K52/5K67

Additional Web Information

Congenic Nomenclature

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

Cardiovascular Research
Hypertension (diet-induced)
Metabolic Syndrome
Other (altered fat metabolism)

References

Selected Reference(s)

Butler AA; Kesterson RA; Khong K; Cullen MJ; Pelleymounter MA; Dekoning J; Baetscher M; Cone RD. 2000. A unique metabolic syndrome causes obesity in the melanocortin-3 receptor-deficient mouse. Endocrinology 141(9):3518-21. [PubMed: 10965927]  [MGI Ref ID J:78132]

Additional References

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Price and Supply Information

Strain Name:	B6.129S4-Mc3rtm1Cone/J
Stock Number:	007809

Price Details

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Supply Details

Standard Supply	Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes	Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.
Licensing	See General Terms and Conditions below for Licensing and Use Restrictions
Control Information	View Control Information in Strain Details.

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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