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Strain Name:

C.129-Stat5btm1Hwd/J

Stock Number:

007810

Availability:

Repository- Live

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General Terms and Conditions

Genes & Alleles   Stat5b;   Stat5btm1Hwd;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Targeted Mutation
Mating SystemHeterozygote x Heterozygote         (Female x Male)
Specieslaboratory mouse
Donating Investigator Michael Tomasson,   Washington University School of Medicine
GenerationN5+ (16-APR-08)

Strain Description
Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA or protein) is detected by Northern and immunoblot analysis of liver, spleen, mammary gland, thymus, kidney or skeletal muscle from homozygous animals. Levels of the closely related Stat5a gene products (mRNA and protein) are unaffected in thymus and spleen. At 4 - 5 weeks of age, male homozygotes are smaller in size and have a reduced body weight (27% lighter than wildtype controls) when compared to wildtype. Female homozygotes exhibit spontaneous abortion between day 8 and 17 of pregnancy and have impaired lactation. Pups (independent of genotype) born to heterozygous females have higher perinatal death than pups born to wildtype females. Some homozygotes have pale and enlarged livers. Homozygotes have less adipose tissue than wildtype controls, exhibit abnormal gene expression in liver, reduced insulin-like growth factor 1 (IGF-1) plasma levels, and disrupted urine chemistry. Male homozygotes have elevated growth hormone plasma levels. Some homozygotes develop obesity at approximately 9 weeks of age with large testicular fat pads and increased abdominal fat. Hair growth is impaired. Homozygotes on the C57BL/6 genetic background (not available from The Jackson Laboratory) have diminished peripheral splenocyte responses to mitogenic stimulation and reduced activated splenocyte proliferation with diminished NK cell number and activity. This mutant mouse strain may be useful in studies of mammary gland development, lactogenesis and immunological intracellular signal transduction.

Strain Development
A targeting vector containing a neomycin resistance cassette and a herpes simplex virus thymidine kinase gene was used to disrupt codon 181. The construct was electroporated into unspecified embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to BALB/c mice, and then backcrossed to BALB/cAnNTac for 4 generations using a marker-assisted protocol.

Mammalian Phenotype Terms assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Stat5btm1Hwd/Stat5btm1Hwd

        involves: C57BL/6
  • immune system phenotype
  • abnormal lymphocyte morphology (MGI Ref ID J:112035)
    • abnormal double-negative T cell morphology (MGI Ref ID J:112035)
      • among the double negative cells, there is a moderate decrease in the percentage of CD44+CD25- cells and an increase in the percentage of CD44-CD25+ cells
    • abnormal lymphocyte cell number (MGI Ref ID J:112035)
      • the T/B cell ratio is slightly decreased
      • decreased CD8-positive T cell number (MGI Ref ID J:112035)
        • exhibit diminished numbers of single positive splenocytes; the number of CD4-CD8+ splenocytes is reduced, whereas, the number of CD4+CD8- cells is only slightly affected
      • decreased NK cell number (MGI Ref ID J:112035)
        • percentage of NK (CD3-DX5+) cells is diminished (about 55% of the percentage seen in wild type)
        • exhibit a decrease in IL2-Rbeta+ NK cells
      • decreased thymocyte number (MGI Ref ID J:112035)
        • thymocyte numbers are modestly diminished
    • decreased T cell proliferation (MGI Ref ID J:112035)
      • splenocytes exhibit greatly diminished proliferation in response to IL-2 and IL-15 and a combination of PMA and IL-4
  • abnormal spleen cellularity (MGI Ref ID J:112035)
    • exhibit decreased numbers of splenocytes in the periphery
  • impaired NK cell cytolysis (MGI Ref ID J:112035)
    • NK cytolytic activity is impaired
  • hematopoietic system phenotype
  • abnormal lymphocyte morphology (MGI Ref ID J:112035)
    • abnormal double-negative T cell morphology (MGI Ref ID J:112035)
      • among the double negative cells, there is a moderate decrease in the percentage of CD44+CD25- cells and an increase in the percentage of CD44-CD25+ cells
    • abnormal lymphocyte cell number (MGI Ref ID J:112035)
      • the T/B cell ratio is slightly decreased
      • decreased CD8-positive T cell number (MGI Ref ID J:112035)
        • exhibit diminished numbers of single positive splenocytes; the number of CD4-CD8+ splenocytes is reduced, whereas, the number of CD4+CD8- cells is only slightly affected
      • decreased NK cell number (MGI Ref ID J:112035)
        • percentage of NK (CD3-DX5+) cells is diminished (about 55% of the percentage seen in wild type)
        • exhibit a decrease in IL2-Rbeta+ NK cells
      • decreased thymocyte number (MGI Ref ID J:112035)
        • thymocyte numbers are modestly diminished
    • decreased T cell proliferation (MGI Ref ID J:112035)
      • splenocytes exhibit greatly diminished proliferation in response to IL-2 and IL-15 and a combination of PMA and IL-4
  • abnormal spleen cellularity (MGI Ref ID J:112035)
    • exhibit decreased numbers of splenocytes in the periphery

Stat5btm1Hwd/Stat5btm1Hwd

        involves: 129 * BALB/c
  • adipose tissue phenotype
  • decreased adipose tissue amount (MGI Ref ID J:41784)
    • both males and females exhibit less adipose tissue
    • weight of sublumbar adipose tissue in females is reduced to 36% of wild type and to 69% of wild type in males
  • endocrine/exocrine gland phenotype
  • abnormal lactation (MGI Ref ID J:41784)
    • produce insufficient milk to feed pups
  • abnormal mammary gland development (MGI Ref ID J:41784)
    • mammary gland development is impaired although milk proteins are expressed
  • growth/size phenotype
  • decreased body weight (MGI Ref ID J:41784)
    • males, but not females, show decreased body weight so that by 4-5 weeks of age, males are 27% lighter than wild type and this difference persists beyond puberty
  • dwarfism (MGI Ref ID J:41784)
  • obese (MGI Ref ID J:41784)
    • obesity becomes apparent in some mutants beginning at about 9 weeks of age, showing large testicular fat pads and greater amounts of abdominal fat
  • homeostasis/metabolism phenotype
  • abnormal urine chemistry (MGI Ref ID J:41784)
    • MUP, a family of alpha2-microglobulin-related liver secretory proteins that comprise a major protein component of mouse urine, levels in male urine are similar to the amounts seen in female urine which is less than seen in wild type males
  • decreased circulating insulin-like growth factor I level (MGI Ref ID J:41784)
    • plasma IGF-1 concentrations are lower in both males and females
  • increased circulating growth hormone level (MGI Ref ID J:41784)
    • males, but not females, exhibit normal to elevated plasma growth hormone levels
  • liver/biliary system phenotype
  • abnormal liver physiology (MGI Ref ID J:41784)
    • male-specific liver gene expression is decreased to wild type female levels in homozygous males, while female-predominant liver gene products are increased in males to a level intermediate between wild type male and female levels
  • enlarged liver (MGI Ref ID J:41784)
    • seen in some homozygotes
  • pale liver (MGI Ref ID J:41784)
    • seen in some homozygotes
  • renal/urinary system phenotype
  • abnormal urine chemistry (MGI Ref ID J:41784)
    • MUP, a family of alpha2-microglobulin-related liver secretory proteins that comprise a major protein component of mouse urine, levels in male urine are similar to the amounts seen in female urine which is less than seen in wild type males
  • reproductive system phenotype
  • abnormal lactation (MGI Ref ID J:41784)
    • produce insufficient milk to feed pups
  • abnormal mammary gland development (MGI Ref ID J:41784)
    • mammary gland development is impaired although milk proteins are expressed
  • abnormal spontaneous abortion rate (MGI Ref ID J:41784)
    • females consistently abort between day 8 and 17 of pregnancy, with no obvious maternal, placental or fetal defects; deaths are not associated with genotype of pups
    • subcutaneous administration of progesterone maintains pregnancy
  • skin/coat/nails phenotype
  • abnormal hair growth (MGI Ref ID J:41784)
    • exhibit a delay of at least 2 weeks in the commencement of second-generation hair growth
    • sparse hair (MGI Ref ID J:41784)
      • seen in some homozygotes

Gene & Allele Details

Allele Symbol Stat5btm1Hwd
Allele Name targeted mutation 1, Helen W Davey
Common Name(s) STAT5b-; Stat5b KO;
Mutation Made By Michael Tomasson,   Washington University School of Medicine
Gene Symbol and Name Stat5b, signal transducer and activator of transcription 5B
Chromosome 11
Gene Common Name(s) STAT5;
Molecular Note The gene was disrupted by insertion of a neomycin resistance cassette into codon 181 via homologous recombination. Absence of gene expression in liver, spleen, mammary gland, thymus, kidney, and skeletal muscle of homozygous mutant animals was demonstrated by Northern and Western blot analyses. [MGI Ref ID J:41784]

Control Information

  Control
   Wild-type from the colony
   000651 BALB/cJ
 
  Considerations for Choosing Controls

Genotyping Protocols

Stat5btm1Hwd

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as heterozygotes. Male homozgotes have a growth defect and female homozygotes have a milk production defect.
Diet Information LabDiet® 5K52/5K67

Additional Web Information

Congenic Nomenclature

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

Developmental Biology Research
Growth Defects Growth Defects (homozygous)

Endocrine Deficiency Research
Adipose Defects
Mammary Gland Defects

Immunology and Inflammation Research
Intracellular Signaling Molecules

References

Selected Reference(s)

Udy GB; Towers RP; Snell RG; Wilkins RJ; Park SH; Ram PA; Waxman DJ ; Davey HW. 1997. Requirement of STAT5b for sexual dimorphism of body growth rates and liver gene expression. Proc Natl Acad Sci U S A 94(14):7239-44. [PubMed: 9207075]  [MGI Ref ID J:41784]

Additional References

Price and Supply Information

Strain Name: C.129-Stat5btm1Hwd/J
Stock Number: 007810

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
LicensingSee General Terms and Conditions below  
Control InformationView Control Information in Strain Details.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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