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| These mice express the histone 2B and Green fluorescent protein, H2BGFP, fusion gene under the control of the endogenous Efnb2 promoter. Homozygotes die at E10.5 due to cardiovascular defects. Homozygous embryos exhibit defects in cardiovascular and somite development, cranial and trunk neural crest cell populations. These mutant mice may be useful in studies of embryonic development. | |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation ?+F1pN1
Generation DefinitionsDonating Investigator Philippe Soriano, Mount Sinai School of Medicine Description
These mice express the histone 2B and Green fluorescent protein, H2BGFP, fusion gene under the control of the endogenous Efnb2 promoter. Homozygotes die at E10.5 due to cardiovascular defects. Homozygous embryos exhibit defects in cardiovascular and somite development, cranial and trunk neural crest cell populations. These mutant mice may be useful in studies of embryonic development.Development
A targeting vector containing the histone 2B and Green Fluorescent Protein, H2BGFP, fusion gene was used to disrupt exon 1 and the start codon of the Efnb2 gene. The construct was electroporated into 129S4/SvJaeSor-derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (240 strains)
006039 B6.129S7-Efnb2tm1And/J 006042 B6.129S7-Efnb2tm2And/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
Efnb2tm2Sor/Efnb2tm2Sor
involves: 129S4/SvJaeSor * C57BL/6J
- lethality-prenatal/perinatal
- embryonic lethality during organogenesis
- die at about E10.5 (MGI Ref ID J:143845)
- embryogenesis phenotype
- abnormal neural crest cell migration
- the streams of cells migrating towards the first and second branchial arches are reduced in size (MGI Ref ID J:143845)
- disruption of trunk migration with scattered migration pattern and loss of normal segmented migration (MGI Ref ID J:143845)
- in the trunk neural crest cells invade the posterior part of somites (MGI Ref ID J:143845)
- abnormal neural crest cells
- abnormal somite development
- impaired differentiation (MGI Ref ID J:143845)
- abnormal anterior-posterior polarity of the somites
- expression analysis suggests a defect in somite patterning (MGI Ref ID J:143845)
- abnormal rostral-caudal patterning of the somites
- small first branchial arch
- nervous system phenotype
- abnormal neural crest cells
- small trigeminal ganglion (MGI Ref ID J:143845)
- craniofacial phenotype
- small first branchial arch
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Efnb2tm2Sor/Efnb2tm2Sor
involves: 129S4/SvJaeSor
- lethality-prenatal/perinatal
- embryonic lethality
- early embryonic lethality occurs in all homozygotes (MGI Ref ID J:115952)
This mouse can be used to support research in many areas including:
Developmental Biology Research
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Efnb2tm2Sor | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Philippe Soriano | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | Efnb2-H2BGFP; Efnb2GFP; ephrin-B2GFP; | ||
| Mutation Made By | Philippe Soriano, Mount Sinai School of Medicine | ||
| Strain of Origin | 129S4/SvJaeSor | ||
| ES Cell Line Name | AK7 | ||
| ES Cell Line Strain | 129S4/SvJaeSor | ||
| Site of Expression | Fluorescence patterns have not been characterized. | ||
| Gene Symbol and Name | Efnb2, ephrin B2 | ||
| Chromosome | 8 | ||
| Gene Common Name(s) | ELF-2; EPLG5; Epl5; HTKL; Htk-L; LERK-5; LERK5; MGC126226; MGC126227; MGC126228; NLERK-1; eph-related receptor tyrosine kinase ligand 5; | ||
| Molecular Note | The H2B-GFP cDNA was inserted into exon 1 replacing a fragment of DNA including the start codon. RT-PCR analysis confirmed the absence of RNA expression in homozygous embryos. [MGI Ref ID J:115952] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Davy A; Bush JO; Soriano P. 2006. Inhibition of gap junction communication at ectopic Eph/ephrin boundaries underlies craniofrontonasal syndrome. PLoS Biol 4(10):e315. [PubMed: 16968134] [MGI Ref ID J:115952]
Additional References
Efnb2tm2Sor relatedDavy A; Soriano P. 2007. Ephrin-B2 forward signaling regulates somite patterning and neural crest cell development. Dev Biol 304(1):182-93. [PubMed: 17223098] [MGI Ref ID J:143845]
Kim YH; Hu H; Guevara-Gallardo S; Lam MT; Fong SY; Wang RA. 2008. Artery and vein size is balanced by Notch and ephrin B2/EphB4 during angiogenesis. Development 135(22):3755-64. [PubMed: 18952909] [MGI Ref ID J:144857]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as heterozygotes. Homozygotes have an embryonic lethal phenotype and die at E10.5 due to cardiovascular defects.
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Tel: 1-800-422-6423 or 1-207-288-5845
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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