Strain Name: |
B6;129S4-Efnb2tm2Sor/J |
|---|---|
Stock Number: |
007843 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Genes & Alleles | Efnb2; Efnb2tm2Sor; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System +/+ sibling x Heterozygote (Female x Male) Species laboratory mouse Donating Investigator Philippe Soriano, Fred Hutchinson Cancer Research Center Generation ?+F1 (25-APR-08) Strain Description
These mice express the histone 2B and Green fluorescent protein, H2BGFP, fusion gene under the control of the endogenous Efnb2 promoter. Homozygotes die at E10.5 due to cardiovascular defects. Homozygous embryos exhibit defects in cardiovascular and somite development, cranial and trunk neural crest cell populations. These mutant mice may be useful in studies of embryonic development.Strain Development
A targeting vector containing the histone 2B and Green Fluorescent Protein, H2BGFP, fusion gene was used to disrupt exon 1 and the start codon of the Efnb2 gene. The construct was electroporated into 129S4/SvJaeSor-derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Efnb2tm2Sor | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Philippe M Soriano | ||
| Common Name(s) | ephrin-B2GFP; | ||
| Mutation Made By | Philippe Soriano, Fred Hutchinson Cancer Research Center | ||
| Strain of Origin | 129S4/SvJaeSor | ||
| ES Cell Line Name | AK7 | ||
| ES Cell Line Strain | 129S4/SvJaeSor | ||
| Gene Symbol and Name | Efnb2, ephrin B2 | ||
| Chromosome | 8 | ||
| Gene Common Name(s) | ELF-2; EPLG5; Epl5; HTKL; Htk-L; LERK-5; LERK5; MGC126226; MGC126227; MGC126228; NLERK-1; eph-related receptor tyrosine kinase ligand 5; | ||
| Molecular Note | The H2BGFP cDNA was inserted into exon 1. [J:115952] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as heterozygotes. Homozygotes have an embryonic lethal phenotype and die at E10.5 due to cardiovascular defects. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (138 strains)
006039 B6.129S7-Efnb2tm1And/J 006042 B6.129S7-Efnb2tm2And/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Developmental Biology Research
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Davy A; Bush JO; Soriano P. 2006. Inhibition of gap junction communication at ectopic Eph/ephrin boundaries underlies craniofrontonasal syndrome. PLoS Biol 4(10):e315. [PubMed: 16968134] [J:115952]
Price and Supply Information
| Strain Name: | B6;129S4-Efnb2tm2Sor/J |
| Stock Number: | 007843 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
USA, Canada and Mexico
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $236.40 | Heterozygous for Efnb2tm2Sor | |||
| Pair | $288.65 | Heterozygous for Efnb2tm2Sor x Wild-type for Efnb2tm2Sor | |||
| Pair | $288.65 | Wild-type for Efnb2tm2Sor x Heterozygous for Efnb2tm2Sor | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-800-422-6423. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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