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Genes & Alleles

Myf5;   Myf5tm3(cre)Sor;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System Heterozygote x +/+ sibling         (Female x Male) Species laboratory mouse Donating Investigator Philippe Soriano,   Fred Hutchinson Cancer Research Center Generation ?+N1 (08-JAN-08)

Strain Description
This strain expresses Cre recombinase from the endogenous Myf5 locus. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs skeletal muscle and the dermis, and in several ectopic locations. Homozygotes for this allele have a perinatal lethal phenotype and die at birth. Homozygotes display abnormal rib development and some fusions of the cervical or thoracic vertebrae. This mutant mouse strain represents a model that may be useful in studies of skeletal development.

Strain Development
A targeting vector containing nuclear localized cre coding sequence, loxP site flanked neomycin resistance cassette and a diphtheria toxin gene was inserted 90 bp upstream of the initiation codon to disrupt the targeted gene. The endogenous Myf5 promoter drives expression of the Cre recombinase. The construct was electroporated into 129S4/SvJaeSor-derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to Cre recombinase expressing strain to remove the neomycin selection cassette and then backcrossed to C57BL/6J mice for one generation.

Mammalian Phenotype Terms assigned by genotype Myf5tm3(cre)Sor/Myf5tm3(cre)Sor         involves: 129S4/SvJae * C57BL/6J skeleton phenotype abnormal rib development (MGI Ref ID J:65301) animals show more rib formation than Myf5tm1Jae homozygotes, but ribs frequently do not extend to the vertebrae cervical vertebral fusion (MGI Ref ID J:65301) fusions are seen in most animals, but are less severe than in Myf5tm1Jae homozygotes thoracic vertebral fusion (MGI Ref ID J:65301) fusions are seen in most animals, but are less severe than in Myf5tm1Jae homozygotes

Gene & Allele Details

Allele Symbol		Myf5tm3(cre)Sor
Allele Name		targeted mutation 3, Philippe Soriano
Common Name(s)		Myf5Cre;
Mutation Made By		Philippe Soriano,   Fred Hutchinson Cancer Research Center
Strain of Origin		129S4/SvJaeSor
ES Cell Line Name		AK7
ES Cell Line Strain		129S4/SvJaeSor
Site of Expression		skeletal muscle, dermis, and several ectopic locations
Gene Symbol and Name		Myf5, myogenic factor 5
Chromosome		10
Gene Common Name(s)		B130010J22Rik; Myf-5; RIKEN cDNA B130010J22 gene; bHLHc2;
Molecular Note		A cre cDNA was inserted between a SacI site and a KpnI site in the 5'UTR of the Myf5 gene. An adjacent loxP-flanked neomycin cassette was removed by Cre- mediated recombination. [MGI Ref ID J:65301]

Control Information

	Control
	Wild-type from the colony
Considerations for Choosing Controls

Genotyping Protocols

Myf5^tm3(cre)Sor

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as heterozygotes. Homozygotes have a perinatal lethal phenotype.
Diet Information	LabDiet® 5K52/5K67

Related Strains

Strains carrying other alleles of Myf5

002522

C.129S4-Myf5tm1Jae/J

View Strains carrying other alleles of Myf5     (1 strain)

Additional Web Information

Cre-lox or FLP-FRT Systems

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

Developmental Biology Research
Perinatal Lethality (Homozygous)
Skeletal Defects

Research Tools
Cre-lox System (Cre Recombinase Expression)
Developmental Biology Research (Cre-lox System)

References

Selected Reference(s)

Tallquist MD; Weismann KE; Hellstrom M; Soriano P. 2000. Early myotome specification regulates PDGFA expression and axial skeleton development Development 127(23):5059-70. [PubMed: 11060232]  [MGI Ref ID J:65301]

Additional References

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Price and Supply Information

Strain Name:	B6;129S4-Myf5tm3(cre)Sor/J
Stock Number:	007845

Price Details

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Supply Details

Standard Supply	Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes	Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.
Licensing	See General Terms and Conditions below for Licensing and Use Restrictions
Control Information	View Control Information in Strain Details.

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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