Strain Name:

129S-Pdgfrbtm1Sor/J

Stock Number:

007846

Order this mouse

Availability:

Cryopreserved - Ready for recovery

Homozygous embryos exhibit purpura, edema, anemia, polychromasia, anisocytosis and abnormal kidney development. Some homozygous embryos do not survive past E18.5. This mutant mouse strain may be useful in studies of hematopoiesis, kidney development and and cellular signaling during development.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
GenerationN?+N1pN1
Generation Definitions
 
Donating InvestigatorDr. Philippe Soriano,   Mount Sinai School of Medicine

Description
One third of homozygous embryos, aged E16 to E18.5, exhibit purpura and edema with some embryos in this group dying at this stage. Homozygous embryos delivered by Caesarean section at E18.5 die within minutes. Homozygotes exhibit anemia, elevated numbers of nucleated erythrocytes, polychromasia, irregularly shaped mature erythrocytes (anisocytosis), and hemorrhaging. Glomerular capillary tufts are absent and the capsule space is filled with blood cells. No gene product (protein) is detected by Western blot analysis of total protein. A truncated transcript presumed to be due to exon skipping is detected by Northern blot analysis. This mutant mouse strain may be useful in studies of hematopoiesis, kidney development and and cellular signaling during development.

Development
A targeting vector containing a PGKneo cassette was used to disrupt 1.8 kb of sequence encoding the signal peptide and the second immunoglobulin domain of the targeted gene.

The construct was electroporated into 129S7/SvEvBrd-Hprt1+ derived AB1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Heterozygous mice were maintained on a 129S4/SvJaeSor background prior to arrival at The Jackson Laboratory. The mice were then crossed to 129S1/SvImJ (Stock No. 002448) for one generation.

Control Information

  Control
   Wild-type from the colony
   002448 129S1/SvImJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Pdgfrb
010977   129S4/SvJae-Pdgfrbtm11Sor/J
018434   STOCK Pdgfrbtm13(Pdgfrb)Sor/J
018435   STOCK Pdgfrbtm14(Pdgfrb)Sor/J
View Strains carrying other alleles of Pdgfrb     (3 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Basal Ganglia Calcification, Idiopathic, 1; IBGC1   (PDGFRB)
Basal Ganglia Calcification, Idiopathic, 4; IBGC4   (PDGFRB)
Myeloproliferative Disorder, Chronic, with Eosinophilia   (PDGFRB)
Myofibromatosis, Infantile, 1; IMF1   (PDGFRB)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Pdgfrbtm1Sor/Pdgfrbtm1Sor

        either: 129S7/SvEvBrd-Pdgfrbtm1Sor or (involves: 129S7/SvEvBrd * C57BL/6J)
  • mortality/aging
  • partial lethality throughout fetal growth and development
    • some embryos are dead around E16-18.5   (MGI Ref ID J:20016)
  • partial perinatal lethality
    • majority of mutants die perinatally   (MGI Ref ID J:20016)
    • cesarean delivered pups die within a matter of minutes of delivery   (MGI Ref ID J:20016)
  • homeostasis/metabolism phenotype
  • hydrops fetalis
    • the 1/3 of embryos that exhibit purpura are also edematous   (MGI Ref ID J:20016)
  • hematopoietic system phenotype
  • abnormal erythrocyte morphology   (MGI Ref ID J:20016)
    • anisocytosis   (MGI Ref ID J:20016)
    • increased nucleated erythrocyte cell number
      • significantly elevated number of nucleated erythrocytes   (MGI Ref ID J:20016)
    • poikilocytosis   (MGI Ref ID J:20016)
      • schistocytosis   (MGI Ref ID J:20016)
    • polychromatophilia   (MGI Ref ID J:20016)
  • anemia   (MGI Ref ID J:20016)
  • decreased platelet cell number   (MGI Ref ID J:20016)
  • cardiovascular system phenotype
  • abnormal venule morphology
    • dilation of veinules   (MGI Ref ID J:20016)
  • decreased glomerular capillary number
    • capillaries within the renal glomerulus are very scarce   (MGI Ref ID J:20016)
  • hemorrhage
    • some cesarean delivered pups suddenly lose most of their blood through the cut in the umbilical cord   (MGI Ref ID J:20016)
    • kidney hemorrhage
      • kidney shows specks of blood   (MGI Ref ID J:20016)
      • blood cells fill up the capsule space   (MGI Ref ID J:20016)
      • glomerulus hemorrhage
        • the capillary tuft normally consisting of podoctyes and mesangial cells is missing in every glomerulus and is replaced with blood cells filling up the capsule space   (MGI Ref ID J:20016)
    • purpura
      • at E16 and E18.5, about 1/3 of embryos exhibit purpura, an accumulation of blood under the surface of the dermis   (MGI Ref ID J:20016)
  • renal/urinary system phenotype
  • absent mesangial cell
    • absence, or significant paucity, of mesangial cells   (MGI Ref ID J:20016)
  • absent podocytes   (MGI Ref ID J:20016)
  • decreased glomerular capillary number
    • capillaries within the renal glomerulus are very scarce   (MGI Ref ID J:20016)
  • kidney hemorrhage
    • kidney shows specks of blood   (MGI Ref ID J:20016)
    • blood cells fill up the capsule space   (MGI Ref ID J:20016)
    • glomerulus hemorrhage
      • the capillary tuft normally consisting of podoctyes and mesangial cells is missing in every glomerulus and is replaced with blood cells filling up the capsule space   (MGI Ref ID J:20016)
  • integument phenotype
  • pallor
    • cesarean delivered pups are pale   (MGI Ref ID J:20016)
  • purpura
    • at E16 and E18.5, about 1/3 of embryos exhibit purpura, an accumulation of blood under the surface of the dermis   (MGI Ref ID J:20016)

Pdgfrbtm1Sor/Pdgfrbtm1Sor

        involves: 129S7/SvEvBrd
  • cardiovascular system phenotype
  • abnormal angiogenesis
    • perivascular cells are missing from angiogenic sprouts   (MGI Ref ID J:138069)
    • decreased angiogenesis
      • ES cells fail to respond to application of Pdgfb by forming a peripheral capillary plexus as wild-type cells do   (MGI Ref ID J:138069)
      • however, formation of Pdgf-independent vascular structures is normal   (MGI Ref ID J:138069)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Pdgfrbtm1Sor/Pdgfrbtm1Sor

        involves: 129S7/SvEvBrd * C57BL/6
  • cardiovascular system phenotype
  • abnormal blood vessel morphology
    • mean blood vessel diameter is increased by 25%   (MGI Ref ID J:78912)
    • exhibit regional dilations along the blood vessel length that are not seen in wild-type   (MGI Ref ID J:78912)
    • abnormal pericyte morphology
      • exhibit loss and altered organization of pericytes in placentas; pericytes are found in the labyrinthine layer but are reduced by about 50% and are completely absent from the spongiotrophoblast layer   (MGI Ref ID J:56682)
      • pericytes are displaced from multicellular core aggregates to scattered sites in the walls of dilated fetal vessels   (MGI Ref ID J:56682)
    • abnormal placenta vasculature
      • fetal vessels are dilated to a varying degree in placentas; seen by E13.5   (MGI Ref ID J:56682)
      • abnormal placental labyrinth vasculature morphology
        • the fetal vessel parametric length and area is increased relative to the maternal lacunas in the labyrinthine layer at E17   (MGI Ref ID J:56682)
    • abnormal vascular endothelial cell morphology
      • the circumferential length of endothelial cell junctions is reduced by 25%, indicating that endothelial cells, on average, are more densely packed in capillary walls   (MGI Ref ID J:78912)
      • increased vascular endothelial cell number
        • exhibit endothelial cell hyperplasia in the brain from E11.5 on, however hyperplasia is not seen in the perineural vascular plexus surrounding the brain   (MGI Ref ID J:78912)
  • embryogenesis phenotype
  • abnormal placenta vasculature
    • fetal vessels are dilated to a varying degree in placentas; seen by E13.5   (MGI Ref ID J:56682)
    • abnormal placental labyrinth vasculature morphology
      • the fetal vessel parametric length and area is increased relative to the maternal lacunas in the labyrinthine layer at E17   (MGI Ref ID J:56682)
  • abnormal trophoblast layer morphology
    • nonendothelial labyrinthine cell number, dominated by the trophoblasts, is reduced by about 40%   (MGI Ref ID J:56682)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Internal/Organ Defects
      kidney
Perinatal Lethality
      Homozygous

Hematological Research
Hematopoietic Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Pdgfrbtm1Sor
Allele Name targeted mutation 1, Philippe Soriano
Allele Type Targeted (Null/Knockout)
Common Name(s) PDGFR-beta-; PDGFRbeta null;
Strain of Origin129S7/SvEvBrd-Hprt<+>
ES Cell Line NameAB1
ES Cell Line Strain129S7/SvEvBrd-Hprt<+>
Gene Symbol and Name Pdgfrb, platelet derived growth factor receptor, beta polypeptide
Chromosome 18
Gene Common Name(s) AI528809; CD140B; IBGC4; IMF1; JTK12; PDGFR; PDGFR-1; PDGFR1; Pdgfr; expressed sequence AI528809; platelet derived growth factor receptor;
Molecular Note A neomycin resistance cassette replaced 1.8 kb of genomic sequence, which encodes part of the signal peptide and the second immunoglobulin domain. Northern blot analysis detected a shorter transcript in homozygous mutant mice that was presumed to be dueto exon skipping. Western blot analysis of total protein failed to detect any encoded protein in homozygous mice. [MGI Ref ID J:20016]

Genotyping

Genotyping Information

Genotyping Protocols

Pdgfrbtm1Sor, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Soriano P. 1994. Abnormal kidney development and hematological disorders in PDGF beta-receptor mutant mice. Genes Dev 8(16):1888-96. [PubMed: 7958864]  [MGI Ref ID J:20016]

Additional References

Pdgfrbtm1Sor related

Basciani S; Mariani S; Arizzi M; Brama M; Ricci A; Betsholtz C; Bondjers C; Ricci G; Catizone A; Galdieri M; Spera G; Gnessi L. 2004. Expression of platelet-derived growth factor (PDGF) in the epididymis and analysis of the epididymal development in PDGF-A, PDGF-B, and PDGF receptor beta deficient mice. Biol Reprod 70(1):168-77. [PubMed: 14522834]  [MGI Ref ID J:89838]

Bell RD; Winkler EA; Sagare AP; Singh I; LaRue B; Deane R; Zlokovic BV. 2010. Pericytes control key neurovascular functions and neuronal phenotype in the adult brain and during brain aging. Neuron 68(3):409-27. [PubMed: 21040844]  [MGI Ref ID J:167754]

Bondjers C; He L; Takemoto M; Norlin J; Asker N; Hellstrom M; Lindahl P; Betsholtz C. 2006. Microarray analysis of blood microvessels from PDGF-B and PDGF-Rbeta mutant mice identifies novel markers for brain pericytes. FASEB J 20(10):1703-5. [PubMed: 16807374]  [MGI Ref ID J:111339]

Chhabra A; Lechner AJ; Ueno M; Acharya A; Van Handel B; Wang Y; Iruela-Arispe ML; Tallquist MD; Mikkola HK. 2012. Trophoblasts regulate the placental hematopoietic niche through PDGF-B signaling. Dev Cell 22(3):651-9. [PubMed: 22387002]  [MGI Ref ID J:182738]

Daneman R; Zhou L; Kebede AA; Barres BA. 2010. Pericytes are required for blood-brain barrier integrity during embryogenesis. Nature 468(7323):562-6. [PubMed: 20944625]  [MGI Ref ID J:166533]

Greif DM; Kumar M; Lighthouse JK; Hum J; An A; Ding L; Red-Horse K; Espinoza FH; Olson L; Offermanns S; Krasnow MA. 2012. Radial construction of an arterial wall. Dev Cell 23(3):482-93. [PubMed: 22975322]  [MGI Ref ID J:189012]

Hellstrom M; Gerhardt H; Kalen M; Li X; Eriksson U; Wolburg H; Betsholtz C. 2001. Lack of pericytes leads to endothelial hyperplasia and abnormal vascular morphogenesis. J Cell Biol 153(3):543-53. [PubMed: 11331305]  [MGI Ref ID J:78912]

Hellstrom M; Kal n M; Lindahl P; Abramsson A; Betsholtz C. 1999. Role of PDGF-B and PDGFR-beta in recruitment of vascular smooth muscle cells and pericytes during embryonic blood vessel formation in the mouse. Development 126(14):3047-55. [PubMed: 10375497]  [MGI Ref ID J:20328]

Klinghoffer RA; Mueting-Nelsen PF; Faerman A; Shani M; Soriano P. 2001. The two PDGF receptors maintain conserved signaling in vivo despite divergent embryological functions. Mol Cell 7(2):343-54. [PubMed: 11239463]  [MGI Ref ID J:67735]

Lindahl P; Hellstrom M; Kalen M; Karlsson L; Pekny M; Pekna M; Soriano P; Betsholtz C. 1998. Paracrine PDGF-B/PDGF-Rbeta signaling controls mesangial cell development in kidney glomeruli. Development 125(17):3313-22. [PubMed: 9693135]  [MGI Ref ID J:50064]

Mellgren AM; Smith CL; Olsen GS; Eskiocak B; Zhou B; Kazi MN; Ruiz FR; Pu WT; Tallquist MD. 2008. Platelet-derived growth factor receptor beta signaling is required for efficient epicardial cell migration and development of two distinct coronary vascular smooth muscle cell populations. Circ Res 103(12):1393-401. [PubMed: 18948621]  [MGI Ref ID J:155272]

Ohlsson R; Falck P; Hellstrom M; Lindahl P; Bostrom H; Franklin G; Ahrlund-Richter L; Pollard J; Soriano P; Betsholtz C. 1999. PDGFB regulates the development of the labyrinthine layer of the mouse fetal placenta. Dev Biol 212(1):124-36. [PubMed: 10419690]  [MGI Ref ID J:56682]

Rolny C; Nilsson I; Magnusson P; Armulik A; Jakobsson L; Wentzel P; Lindblom P; Norlin J; Betsholtz C; Heuchel R; Welsh M; Claesson-Welsh L. 2006. Platelet-derived growth factor receptor-beta promotes early endothelial cell differentiation. Blood 108(6):1877-86. [PubMed: 16690964]  [MGI Ref ID J:138069]

Schmahl J; Raymond CS; Soriano P. 2007. PDGF signaling specificity is mediated through multiple immediate early genes. Nat Genet 39(1):52-60. [PubMed: 17143286]  [MGI Ref ID J:117491]

Schmahl J; Rizzolo K; Soriano P. 2008. The PDGF signaling pathway controls multiple steroid-producing lineages. Genes Dev 22(23):3255-67. [PubMed: 19056881]  [MGI Ref ID J:142042]

Silva RL; Thornton JD; Martin AC; Rehg JE; Bertwistle D; Zindy F; Skapek SX. 2005. Arf-dependent regulation of Pdgf signaling in perivascular cells in the developing mouse eye. EMBO J 24(15):2803-14. [PubMed: 16037818]  [MGI Ref ID J:101016]

Tallquist MD; French WJ; Soriano P. 2003. Additive Effects of PDGF Receptor beta Signaling Pathways in Vascular Smooth Muscle Cell Development. PLoS Biol 1(2):288-99. [PubMed: 14624252]  [MGI Ref ID J:86542]

Van den Akker NM; Winkel LC; Nisancioglu MH; Maas S; Wisse LJ; Armulik A; Poelmann RE; Lie-Venema H; Betsholtz C; Gittenberger-de Groot AC. 2008. PDGF-B signaling is important for murine cardiac development: Its role in developing atrioventricular valves, coronaries, and cardiac innervation. Dev Dyn 237(2):494-503. [PubMed: 18213589]  [MGI Ref ID J:130159]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as heterozygotes. Homozygotes have a perinatal lethal phenotype.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   002448 129S1/SvImJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.8)