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| The mice express a synaptotagmin 1-enhanced cyan fluorescent protein fusion protein under the control of a Thy1 promoter. Fluorescence is observed throughout the brain and is localized to synapses, marking synaptic vesicles without participating in exocytosis. This strain may be useful in monitoring synaptic vesicle traffic in vivo. | |||||||||||||||||||
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B6SJL-Tg(Thy1-Syt1-ECFP)/1SudJ (Changed: 04-DEC-07 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation ?pN1
Generation DefinitionsDonating Investigator Dr. Thomas C. Sudhof, Stanford University School of Medicine Description
Mice homozygous for this transgene are viable and fertile and do not display any gross physical or behavioral abnormalities. The mice express a synaptotagmin 1-enhanced cyan fluorescent protein fusion protein under the control of a Thy1 promoter. Fluorescence is observed throughout the brain and is localized to synapses, marking synaptic vesicles without participating in exocytosis. This strain may be useful in monitoring synaptic vesicle traffic in vivo.
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (357 strains)
004218 B6.129(ICR)-Tg(CAG-ECFP)CK6Nagy/J 008513 B6.129P2-Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde/J 007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J 007911 B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J 007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J 003710 B6.Cg-Tg(Thy1-CFP)23Jrs/J 014131 B6.Cg-Tg(Thy1-CFP)IJrs/GfngJ 007940 B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J 007967 B6.Cg-Tg(Thy1-CFP/COX8A)S2Lich/J 013161 B6.Cg-Tg(Thy1-Clomeleon)1Gjau/J 007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J 008004 B6;SJL-Tg(Thy1-ECFP/VAMP2)1Sud/J 018671 D2.Cg-Tg(Thy1-CFP)23Jrs/SjJ 018067 FVB-Tg(Prism)1849Htz/J 018068 FVB-Tg(Prism)1989Htz/J 007879 STOCK Stx1atm2Sud/J 003773 STOCK Tg(CAG-ECFP)CK6Nagy/J 006784 STOCK Tg(Ins1-Cerulean)24Hara/J 016922 STOCK Tg(Myh7-CFP)1Jrs/J 013162 STOCK Tg(Thy1-Clomeleon)12Gjau/J 013163 STOCK Tg(Thy1-Clomeleon)13Gjau/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Neurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Thy1-Syt1/ECFP)1Sud | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Thomas C Sudhof | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | Syt1 transgenic; TMC; Tg(Thy1-Syt1-ECFP)1Sud; | ||
| Strain of Origin | (C57BL/6 x SJL)F1 | ||
| Site of Expression | ECFP expression is observed throughout the brain and is localized to synapses, marking synaptic vesicles. | ||
| Expressed Gene | Syt1, synaptotagmin I, rat | ||
| Expressed Gene | CFP, Cyan Fluorescent Protein, jellyfish | ||
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory | ||
| Molecular Note | A transgenic vector was designed to create a rat synaptotagmin 1/enhanced cyan fluorescent protein (ECFP) fusion protein under the control of a mouse Thy1 promoter lacking exon3 and the flanking introns (to make gene expression neuron-specific). Expression is detected only in the brain with fluorescence observed throughout the brain, marking synaptic vesicles. [MGI Ref ID J:105052] | ||
| Gene Symbol and Name | Tg(Thy1-Syt1/ECFP)1Sud, transgene insertion 1, Thomas C Sudhof | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Syt1 transgenic; TMC; Tg(Thy1-Syt1-ECFP)1Sud; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Thy1-Syt1/ECFP)1Sud, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Han W; Rhee JS; Maximov A; Lin W; Hammer RE; Rosenmund C; Sudhof TC. 2005. C-terminal ECFP fusion impairs synaptotagmin 1 function: crowding out synaptotagmin 1. J Biol Chem 280(6):5089-100. [PubMed: 15561725] [MGI Ref ID J:105052]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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JAX® Mice, Products & Services Conditions of Use
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