Description

Strain Information

Type Coisogenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System +/+ sibling x Heterozygote         (Female x Male) Species laboratory mouse Generation ?+F2 (09-OCT-08)   Donating Investigator Allen Bale,   Yale University

Description
Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous null mice have an embryonic lethal phenotype, failing to develop past embryonic days 9.5 to 10. No gene product (protein) is detected by Western blot analysis. Truncated gene product (mRNA) is detected by Northern blot analysis. Homozygous embryos at 9 to 9.5 embryonic day exhibit growth retardation, incomplete embryonic turning, abnormal somite development, abnormal heart looping (due to abnormal left-right axial patterning) and open neural tubes. This mutant mouse strain may be useful in studies of the Hedgehog signaling pathway and embryonic development.

Development
A targeting vector containing a loxP site flanked (floxed) neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt the intron 6/exon 7 junction and part of exon 7. The construct was electroporated into 129S1/Sv-p⁺ Tyr⁺ Kitl⁺ derived W9.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric male animals were crossed to 129S1 female mice. The strain was maintained as heterozygotes on the 129S1 background prior to arriving at The Jackson Laboratory.

Control Information

	Control
	Wild-type from the colony
	002448 129S1/SvImJ	(approximate)
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Sufu^tm1Aeb/Sufu⁺

        either: 129S1/Sv-Sufu^tm1Aeb or (involves: 129S1/Sv * C57BL/6)

• normal phenotype
• no abnormal phenotype detected (MGI Ref ID J:101731)
  • exhibit no obvious abnormalities, internal malformations or tumor predisposition

Sufu^tm1Aeb/Sufu^tm1Aeb

        either: 129S1/Sv-Sufu^tm1Aeb or (involves: 129S1/Sv * C57BL/6)

• lethality-prenatal/perinatal
• embryonic lethality during organogenesis (MGI Ref ID J:101731)
  • do not survive past E10.5
• embryogenesis phenotype
• abnormal developmental patterning (MGI Ref ID J:101731)
• abnormal dorsal-ventral axis patterning (MGI Ref ID J:101731)
• abnormal left-right axis patterning (MGI Ref ID J:101731)
  • abnormal heart looping indicates abnormal development of left-right asymmetry
• abnormal primitive streak morphology (MGI Ref ID J:101731)
  • abnormal node architecture at E7.75, ranging from mild changes in the shape of the node to irregular distribution of nodal cells
• incomplete embryo turning (MGI Ref ID J:101731)
  • by E9-9.5, fail to undergo embryo turning
• abnormal somite development (MGI Ref ID J:101731)
  • observed by E9-9.5
• embryonic growth retardation (MGI Ref ID J:101731)
  • embryonic growth retardation is observed by E9-9.5 but not at E8.5
• nervous system phenotype
• open neural tube (MGI Ref ID J:101731)
• cardiovascular system phenotype
• abnormal aorta morphology (MGI Ref ID J:101731)
  • descending aorta is dilated
• abnormal cardiac muscle morphology (MGI Ref ID J:101731)
  • myocardium hyperplasia
• abnormal direction of looping morphogenesis (MGI Ref ID J:101731)
  • 4 of 13 have L-looped (leftward) hearts at E9.5-10.5 and one has indeterminate cardiac looping
• aneurysm (MGI Ref ID J:101731)
  • 4 of 14 have aneurysms in the diencephalic hemispheres
• hemorrhage (MGI Ref ID J:101731)
  • 4 of 14 have hemorrhage in the diencephalic hemispheres
• muscle phenotype
• abnormal cardiac muscle morphology (MGI Ref ID J:101731)
  • myocardium hyperplasia
• growth/size phenotype
• embryonic growth retardation (MGI Ref ID J:101731)
  • embryonic growth retardation is observed by E9-9.5 but not at E8.5

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Developmental Biology Research
Embryonic Lethality (Homozygous)
Growth Defects
Internal/Organ Defects (heart)
Neural Tube Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Sufutm1Aeb
Allele Name		targeted mutation 1, Allen E Bale
Allele Type		Targeted (knock-out)
Mutation Made By		Allen Bale,   Yale University
Strain of Origin		129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
ES Cell Line Name		W9.5/W95
ES Cell Line Strain		129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
Gene Symbol and Name		Sufu, suppressor of fused homolog (Drosophila)
Chromosome		19
Gene Common Name(s)		2810026F04Rik; PRO1280; RIKEN cDNA 2810026F04 gene; SUFUH; SUFUXL; Su(Fu);
Molecular Note		Vector contains exon 4 through the 3' end of intron 6 then PGK-driven NEO in orientation reverse to the gene followed by the 3' end of exon 7 through the end of exon 8. Correct targeting replaces the intron 6/exon 7 splice site and a portion of exon 7 with the neo casette. Northern and Western blot analysis confirmed the absence of transcription and translation. [MGI Ref ID J:101731]

Genotyping

Genotyping Information

Genotyping Protocols

Sufu^tm1Aeb, SEP PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Cooper AF; Yu KP; Brueckner M; Brailey LL; Johnson L; McGrath JM; Bale AE. 2005. Cardiac and CNS defects in a mouse with targeted disruption of suppressor of fused. Development 132(19):4407-17. [PubMed: 16155214]  [MGI Ref ID J:101731]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice are bred as heterozygotes. Homozygotes do not survive past embryonic day 10.5.
Mating System	+/+ sibling x Heterozygote         (Female x Male)
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	Wild-type from the colony
	002448 129S1/SvImJ	(approximate)
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.