Strain Name: |
C57BL/6J-Tg(Eno2-YFP/Cox8a)ZRwb/J |
|---|---|
Stock Number: |
007860 |
Availability: | Under Development for Distribution Colony |
| To register your interest in this strain go to the Strain Interest Form. |
General Terms and Conditions |
| Genes & Alleles | Eno2; YFP; Tg(Eno2-YFP/Cox8a)ZRwb; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Strain Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Rob Burgess, The Jackson Laboratory Strain Description
These transgenic mice express Yellow Fluorescent Protein (YFP) under the control of the neuron specific rat enolase 2, gamma, Eno2, promoter. YFP is specifically localized to the mitochondria by a mouse cytochrome c oxidase, subunit VIIIa, gene targeting signal fused to the N-terminus. Fluorescence is detected in many neuronal populations. The donating investigator reports that fluorescence is detected in a subset of cerebellar purkinje cells, a subset of retinal neurons including ganglion cells, interneurons, and photoreceptors, a subset of dorsal root ganglion sensory neurons, axons of the dorsal funiculus/cortical spinal tract, and CA regions of the hippocampus. This transgene insertion location is on the X Chromosome, based on inheritance. It is not known if homozygotes are viable. This mutant mouse strain may be useful in studies of mitochondrial transport.Strain Development
A transgenic construct containing the entire open reading frame of the Yellow Fluorescent Protein gene fused in-frame with the mitochondrial targeting sequence of the mouse cytochrome c oxidase, subunit VIIIa, gene under the direction of regulatory elements derived from the rat enolase 2, gamma gene, Eno2, was injected into fertilized C57BL/6J donor mouse eggs. Founder line 401, MitoZ, corresponding to the published line Z, was subsequently established.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Eno2-YFP/Cox8a)ZRwb | ||
|---|---|---|---|
| Allele Name | transgenic insertion Z, Robert Burgess | ||
| Common Name(s) | 401; MitoZ; | ||
| Strain of Origin | C57BL/6J | ||
| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish | ||
| Yellow Fluorescent Protein (YFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When YFP is utilized in a transgenic construct, tissue expressing sufficient amounts of YFP will fluoresce yellowish-green when exposed to a 513 nm light source. | |||
| Promoter | Eno2, enolase 2, gamma, neuronal, rat | ||
| Molecular Note | A transgenic construct containing the entire open reading frame of the Yellow Fluorescent Protein gene fused in-frame with the mitochondrial targeting sequence of the mouse cytochrome c oxidase, subunit VIIIa, gene under the direction of regulatory elements derived from the rat enolase 2, gamma gene, Eno2, was injected into fertilized C57BL/6J donor mouse eggs. Founder line Z was established. YFP is specifically localized to the mitochondria by a mouse cytochrome c oxidase, subunit VIIIa, gene targeting signal fused to the N-terminus. Fluorescence is detected in many neuronal populations. [MGI Ref ID J:122259] | ||
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice can be bred as hemizygotes. It is not known if homozygotes are viable. This transgene insertion location is on the X Chromosome, based on inheritance. |
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Fluorescent Protein Strains
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005483 129-Tg(CAG-EYFP)7AC5Nagy/J 006148 B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J 007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J 007911 B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J 007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J 007612 B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J 007615 B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J 005630 B6.Cg-Tg(Thy1-EYFP)15Jrs/J 003709 B6.Cg-Tg(Thy1-YFP)16Jrs/J 005627 B6.Cg-Tg(Thy1-YFP/Syp)10Jrs/J 003782 B6.Cg-Tg(Thy1-YFPH)2Jrs/J 007606 B6.Cg-Tg(Thy1-cre/ESR1,-EYFP)AGfng/J 007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J 005620 B6;D2-Tg(S100B-EYFP)1Wjt/J 007610 B6;SJL-Tg(Thy1-cre/ESR1,-EYFP)VGfng/J 007880 B6SJL-Tg(Thy1-Stx1a/EYFP)1Sud/J 006618 C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J 006362 C57BL/6J-Tg(CMV-Cox8a/EYFP)17J/J 005130 STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Research Applications
This mouse can be used to support research in many areas including:
YFP relatedResearch Tools
Cell Biology Research
Fluorescent Proteins
Neurobiology Research
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Misgeld T; Kerschensteiner M; Bareyre FM; Burgess RW; Lichtman JW. 2007. Imaging axonal transport of mitochondria in vivo. Nat Methods 4(7):559-561. [PubMed: 17558414] [MGI Ref ID J:122259]
Price and Supply Information
| Strain Name: | C57BL/6J-Tg(Eno2-YFP/Cox8a)ZRwb/J |
| Stock Number: | 007860 |
This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date:
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development
Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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